Colonies of the fungus Aspergillus niger are highly differentiated to adapt to local carbon source variation
Abstract
Saprobic fungi, such as Aspergillus niger, grow as colonies consisting of a network ofbranching and fusing hyphae that are often considered to be relatively uniform entities inwhich nutrients can freely move through the hyphae. In nature, different parts of a colony areoften exposed to different nutrients. We have investigated, using a multi-omics approach,adaptation of A. niger colonies to spatially separated and compositionally different plantbiomass substrates. This demonstrated a high level of intra-colony differentiation, whichclosely matched the locally available substrate. The part of the colony exposed to pectin-richsugar beet pulp and to xylan-rich wheat bran showed high pectinolytic and high xylanolytictranscript and protein levels, respectively.This study therefore exemplifies the high ability of fungal colonies to differentiate and adaptto local conditions, ensuring efficient use of the available nutrients, rather than maintaining auniform physiology throughout the colony.
- Authors:
-
- Univ. of Utrecht (Netherlands)
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Wageningen Univ. (The Netherlands)
- Univ. of Helsinki (Finland)
- Publication Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1604781
- Report Number(s):
- PNNL-SA-150305
Journal ID: ISSN 1462-2912
- Grant/Contract Number:
- AC05-76RL01830
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Environmental Microbiology
- Additional Journal Information:
- Journal Volume: 22; Journal Issue: 3; Journal ID: ISSN 1462-2912
- Publisher:
- Wiley
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Daly, Paul, Peng, Mao, Mitchell, Hugh D., Kim, Young-Mo, Ansong, Charles K., Brewer, Heather M., De Gijsel, Peter, Lipton, Mary S., Markillie, Lye Meng, Nicora, Carrie D., Orr, Galya, Wiebenga, Ad, Hilden, Kristiina S., Kabel, Mirjam A., Baker, Scott E., Makela, Miia, and De Vries, Ronald P. Colonies of the fungus Aspergillus niger are highly differentiated to adapt to local carbon source variation. United States: N. p., 2019.
Web. doi:10.1111/1462-2920.14907.
Daly, Paul, Peng, Mao, Mitchell, Hugh D., Kim, Young-Mo, Ansong, Charles K., Brewer, Heather M., De Gijsel, Peter, Lipton, Mary S., Markillie, Lye Meng, Nicora, Carrie D., Orr, Galya, Wiebenga, Ad, Hilden, Kristiina S., Kabel, Mirjam A., Baker, Scott E., Makela, Miia, & De Vries, Ronald P. Colonies of the fungus Aspergillus niger are highly differentiated to adapt to local carbon source variation. United States. https://doi.org/10.1111/1462-2920.14907
Daly, Paul, Peng, Mao, Mitchell, Hugh D., Kim, Young-Mo, Ansong, Charles K., Brewer, Heather M., De Gijsel, Peter, Lipton, Mary S., Markillie, Lye Meng, Nicora, Carrie D., Orr, Galya, Wiebenga, Ad, Hilden, Kristiina S., Kabel, Mirjam A., Baker, Scott E., Makela, Miia, and De Vries, Ronald P. Tue .
"Colonies of the fungus Aspergillus niger are highly differentiated to adapt to local carbon source variation". United States. https://doi.org/10.1111/1462-2920.14907. https://www.osti.gov/servlets/purl/1604781.
@article{osti_1604781,
title = {Colonies of the fungus Aspergillus niger are highly differentiated to adapt to local carbon source variation},
author = {Daly, Paul and Peng, Mao and Mitchell, Hugh D. and Kim, Young-Mo and Ansong, Charles K. and Brewer, Heather M. and De Gijsel, Peter and Lipton, Mary S. and Markillie, Lye Meng and Nicora, Carrie D. and Orr, Galya and Wiebenga, Ad and Hilden, Kristiina S. and Kabel, Mirjam A. and Baker, Scott E. and Makela, Miia and De Vries, Ronald P.},
abstractNote = {Saprobic fungi, such as Aspergillus niger, grow as colonies consisting of a network ofbranching and fusing hyphae that are often considered to be relatively uniform entities inwhich nutrients can freely move through the hyphae. In nature, different parts of a colony areoften exposed to different nutrients. We have investigated, using a multi-omics approach,adaptation of A. niger colonies to spatially separated and compositionally different plantbiomass substrates. This demonstrated a high level of intra-colony differentiation, whichclosely matched the locally available substrate. The part of the colony exposed to pectin-richsugar beet pulp and to xylan-rich wheat bran showed high pectinolytic and high xylanolytictranscript and protein levels, respectively.This study therefore exemplifies the high ability of fungal colonies to differentiate and adaptto local conditions, ensuring efficient use of the available nutrients, rather than maintaining auniform physiology throughout the colony.},
doi = {10.1111/1462-2920.14907},
journal = {Environmental Microbiology},
number = 3,
volume = 22,
place = {United States},
year = {Tue Aug 06 00:00:00 EDT 2019},
month = {Tue Aug 06 00:00:00 EDT 2019}
}
Web of Science
Works referenced in this record:
MetaboAnalyst 3.0—making metabolomics more meaningful
journal, April 2015
- Xia, Jianguo; Sinelnikov, Igor V.; Han, Beomsoo
- Nucleic Acids Research, Vol. 43, Issue W1
Woronin body-based sealing of septal pores
journal, December 2017
- Steinberg, Gero; Harmer, Nicholas J.; Schuster, Martin
- Fungal Genetics and Biology, Vol. 109
Carbohydrate utilization and metabolism is highly differentiated in Agaricus bisporus
journal, January 2013
- Patyshakuliyeva, Aleksandrina; Jurak, Edita; Kohler, Annegret
- BMC Genomics, Vol. 14, Issue 1
A broader role for AmyR in Aspergillus niger: regulation of the utilisation of d-glucose or d-galactose containing oligo- and polysaccharides
journal, August 2011
- vanKuyk, Patricia A.; Benen, Jaques A. E.; Wösten, Han A. B.
- Applied Microbiology and Biotechnology, Vol. 93, Issue 1
Posttranscriptional Control of Gene Expression in Filamentous Fungi
journal, March 1998
- Sachs, Matthew S.
- Fungal Genetics and Biology, Vol. 23, Issue 2
Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans
journal, March 2018
- Khosravi, Claire; Battaglia, Evy; Kun, Roland S.
- BMC Genomics, Vol. 19, Issue 1
Fungal enzyme sets for plant polysaccharide degradation
journal, July 2011
- van den Brink, Joost; de Vries, Ronald P.
- Applied Microbiology and Biotechnology, Vol. 91, Issue 6, p. 1477-1492
Combinatorial control of gene expression in Aspergillus niger grown on sugar beet pectin
journal, September 2017
- Kowalczyk, Joanna E.; Lubbers, Ronnie J. M.; Peng, Mao
- Scientific Reports, Vol. 7, Issue 1
Dichomitus squalens partially tailors its molecular responses to the composition of solid wood : Responses of
journal, October 2018
- Daly, Paul; López, Sara Casado; Peng, Mao
- Environmental Microbiology, Vol. 20, Issue 11
Penicillium subrubescens is a promising alternative for Aspergillus niger in enzymatic plant biomass saccharification
journal, December 2016
- Mäkelä, Miia R.; Mansouri, Sadegh; Wiebenga, Ad
- New Biotechnology, Vol. 33, Issue 6
A new self-assembled peroxisomal vesicle required for efficient resealing of the plasma membrane
journal, March 2000
- Jedd, Gregory; Chua, Nam-Hai
- Nature Cell Biology, Vol. 2, Issue 4
Chemically Etched Open Tubular and Monolithic Emitters for Nanoelectrospray Ionization Mass Spectrometry
journal, November 2006
- Kelly, Ryan T.; Page, Jason S.; Luo, Quanzhou
- Analytical Chemistry, Vol. 78, Issue 22, p. 7796-7801
Genetic analysis and the construction of master strains for assignment of genes to six linkage groups in Aspergillus niger
journal, November 1988
- Bos, C. J.; Debets, A. J. M.; Swart, K.
- Current Genetics, Vol. 14, Issue 5
Datura genome reveals duplications of psychoactive alkaloid biosynthetic genes and high mutation rate following tissue culture
journal, March 2021
- Rajewski, Alex; Carter-House, Derreck; Stajich, Jason
- BMC Genomics, Vol. 22, Issue 1
Biological solutions to transport network design
journal, July 2007
- Bebber, Daniel P.; Hynes, Juliet; Darrah, Peter R.
- Proceedings of the Royal Society B: Biological Sciences, Vol. 274, Issue 1623
Redistribution of soil water by a saprotrophic fungus enhances carbon mineralization
journal, November 2015
- Guhr, Alexander; Borken, Werner; Spohn, Marie
- Proceedings of the National Academy of Sciences, Vol. 112, Issue 47
DAnTE: a statistical tool for quantitative analysis of -omics data
journal, May 2008
- Polpitiya, A. D.; Qian, W. -J.; Jaitly, N.
- Bioinformatics, Vol. 24, Issue 13
The gold-standard genome of Aspergillus niger NRRL 3 enables a detailed view of the diversity of sugar catabolism in fungi
journal, September 2018
- Aguilar-Pontes, M. V.; Brandl, J.; McDonnell, E.
- Studies in Mycology, Vol. 91
NCBI GEO: archive for functional genomics data sets—update
journal, November 2012
- Barrett, Tanya; Wilhite, Stephen E.; Ledoux, Pierre
- Nucleic Acids Research, Vol. 41, Issue D1
Analysis of fungal networks
journal, April 2012
- Heaton, Luke; Obara, Boguslaw; Grau, Vincente
- Fungal Biology Reviews, Vol. 26, Issue 1
Bidirectional Propagation of Signals and Nutrients in Fungal Networks via Specialized Hyphae
text, January 2019
- Schmieder, Stefanie S.; Stanley, Claire E.; Rzepiela, Andrzej
- ETH Zurich
Advances in proteomics data analysis and display using an accurate mass and time tag approach
journal, January 2006
- Zimmer, Jennifer S. D.; Monroe, Matthew E.; Qian, Wei-Jun
- Mass Spectrometry Reviews, Vol. 25, Issue 3
Spatial differentiation of gene expression in Aspergillus niger colony grown for sugar beet pulp utilization
journal, August 2015
- Benoit, Isabelle; Zhou, Miaomiao; Vivas Duarte, Alexandra
- Scientific Reports, Vol. 5, Issue 1
Expression-based clustering of CAZyme-encoding genes of Aspergillus niger
journal, November 2017
- Gruben, Birgit S.; Mäkelä, Miia R.; Kowalczyk, Joanna E.
- BMC Genomics, Vol. 18, Issue 1
Hyphal homing, fusion and mycelial interconnectedness
journal, March 2004
- Glass, N.
- Trends in Microbiology, Vol. 12, Issue 3
The carbohydrate-active enzymes database (CAZy) in 2013
journal, November 2013
- Lombard, Vincent; Golaconda Ramulu, Hemalatha; Drula, Elodie
- Nucleic Acids Research, Vol. 42, Issue D1
HTSeq--a Python framework to work with high-throughput sequencing data
journal, September 2014
- Anders, S.; Pyl, P. T.; Huber, W.
- Bioinformatics, Vol. 31, Issue 2
Cytoplasmic Continuity Revisited: Closure of Septa of the Filamentous Fungus Schizophyllum commune in Response to Environmental Conditions
journal, June 2009
- van Peer, Arend F.; Müller, Wally H.; Boekhout, Teun
- PLoS ONE, Vol. 4, Issue 6
A new self-assembled peroxisomal vesicle required for efficient resealing of the plasma membrane
journal, March 2000
- Jedd, Gregory; Chua, Nam-Hai
- Nature Cell Biology, Vol. 2, Issue 4
Penicillium subrubescens is a promising alternative for Aspergillus niger in enzymatic plant biomass saccharification
journal, December 2016
- Mäkelä, Miia R.; Mansouri, Sadegh; Wiebenga, Ad
- New Biotechnology, Vol. 33, Issue 6
Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88
journal, January 2007
- Pel, Herman J.; de Winde, Johannes H.; Archer, David B.
- Nature Biotechnology, Vol. 25, Issue 2, p. 221-231
Wheat Bran Proteins: A Review of Their Uses and Potential
journal, February 2015
- Balandrán-Quintana, René Renato; Mercado-Ruiz, Jorge Nemesio; Mendoza-Wilson, Ana María
- Food Reviews International, Vol. 31, Issue 3
2016 update of the PRIDE database and its related tools
journal, September 2016
- Vizcaíno, Juan Antonio; Csordas, Attila; del-Toro, Noemi
- Nucleic Acids Research, Vol. 44, Issue 22
Localization of growth and secretion of proteins in Aspergillus niger
journal, August 1991
- Wosten, H. A. B.; Moukha, S. M.; Sietsma, J. H.
- Journal of General Microbiology, Vol. 137, Issue 8
Expression of Aspergillus niger CAZymes is determined by compositional changes in wheat straw generated by hydrothermal or ionic liquid pretreatments
journal, February 2017
- Daly, Paul; van Munster, Jolanda M.; Blythe, Martin J.
- Biotechnology for Biofuels, Vol. 10, Issue 1
Redistribution of soil water by a saprotrophic fungus enhances carbon mineralization
journal, November 2015
- Guhr, Alexander; Borken, Werner; Spohn, Marie
- Proceedings of the National Academy of Sciences, Vol. 112, Issue 47
Blocking hexose entry into glycolysis activates alternative metabolic conversion of these sugars and upregulates pentose metabolism in Aspergillus nidulans
journal, March 2018
- Khosravi, Claire; Battaglia, Evy; Kun, Roland S.
- BMC Genomics, Vol. 19, Issue 1
Spatial Differentiation in the Vegetative Mycelium of Aspergillus niger
journal, October 2007
- Levin, Ana M.; de Vries, Ronald P.; Conesa, Ana
- Eukaryotic Cell, Vol. 6, Issue 12
A broader role for AmyR in Aspergillus niger: regulation of the utilisation of d-glucose or d-galactose containing oligo- and polysaccharides
journal, August 2011
- vanKuyk, Patricia A.; Benen, Jaques A. E.; Wösten, Han A. B.
- Applied Microbiology and Biotechnology, Vol. 93, Issue 1
Switching from a Unicellular to Multicellular Organization in an Aspergillus niger Hypha
journal, March 2015
- Bleichrodt, Robert-Jan; Hulsman, Marc; Wösten, Han A. B.
- mBio, Vol. 6, Issue 2
Combinatorial control of gene expression in Aspergillus niger grown on sugar beet pectin
journal, September 2017
- Kowalczyk, Joanna E.; Lubbers, Ronnie J. M.; Peng, Mao
- Scientific Reports, Vol. 7, Issue 1
Chemically Etched Open Tubular and Monolithic Emitters for Nanoelectrospray Ionization Mass Spectrometry
journal, November 2006
- Kelly, Ryan T.; Page, Jason S.; Luo, Quanzhou
- Analytical Chemistry, Vol. 78, Issue 22, p. 7796-7801
Regulators of plant biomass degradation in ascomycetous fungi
journal, June 2017
- Benocci, Tiziano; Aguilar-Pontes, Maria Victoria; Zhou, Miaomiao
- Biotechnology for Biofuels, Vol. 10, Issue 1
MetaboAnalyst 3.0—making metabolomics more meaningful
journal, April 2015
- Xia, Jianguo; Sinelnikov, Igor V.; Han, Beomsoo
- Nucleic Acids Research, Vol. 43, Issue W1
Dichomitus squalens partially tailors its molecular responses to the composition of solid wood : Responses of
journal, October 2018
- Daly, Paul; López, Sara Casado; Peng, Mao
- Environmental Microbiology, Vol. 20, Issue 11
Fungal Biology: Bidirectional Communication across Fungal Networks
journal, February 2019
- Roper, Marcus; Dressaire, Emilie
- Current Biology, Vol. 29, Issue 4
DAnTE: a statistical tool for quantitative analysis of -omics data
journal, May 2008
- Polpitiya, A. D.; Qian, W. -J.; Jaitly, N.
- Bioinformatics, Vol. 24, Issue 13
How does a hypha grow? The biophysics of pressurized growth in fungi
journal, June 2011
- Lew, Roger R.
- Nature Reviews Microbiology, Vol. 9, Issue 7
Bidirectional Propagation of Signals and Nutrients in Fungal Networks via Specialized Hyphae
journal, January 2019
- Schmieder, Stefanie S.; Stanley, Claire E.; Rzepiela, Andrzej
- Current Biology, Vol. 29, Issue 2
NCBI GEO: archive for functional genomics data sets—update
journal, November 2012
- Barrett, Tanya; Wilhite, Stephen E.; Ledoux, Pierre
- Nucleic Acids Research, Vol. 41, Issue D1
Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2
journal, December 2014
- Love, Michael I.; Huber, Wolfgang; Anders, Simon
- Genome Biology, Vol. 15, Issue 12
Carbohydrate utilization and metabolism is highly differentiated in Agaricus bisporus
journal, January 2013
- Patyshakuliyeva, Aleksandrina; Jurak, Edita; Kohler, Annegret
- BMC Genomics, Vol. 14, Issue 1
Designer synthetic media for studying microbial-catalyzed biofuel production
text, January 2015
- Chundawat, Shishir P. S.; Chambliss, Charles Kevin; Lau, Ming W.
- Rutgers University
The Structure and Function of the Vegetative Mycelium of Ectomycorrhizal Plants. i. Translocation of 14c-Labelled Carbon Between Plants Interconnected by a Common Mycelium
journal, May 1986
- Finlay, R. D.; Read, D. J.
- New Phytologist, Vol. 103, Issue 1
Physiological Significance of Network Organization in Fungi
journal, September 2012
- Simonin, Anna; Palma-Guerrero, Javier; Fricker, Mark
- Eukaryotic Cell, Vol. 11, Issue 11
Biological solutions to transport network design
journal, July 2007
- Bebber, Daniel P.; Hynes, Juliet; Darrah, Peter R.
- Proceedings of the Royal Society B: Biological Sciences, Vol. 274, Issue 1623
FactoMineR : An R Package for Multivariate Analysis
journal, January 2008
- Lê, Sébastien; Josse, Julie; Husson, François
- Journal of Statistical Software, Vol. 25, Issue 1
Mycelial density profiles of fungi on heterogeneous media and their interpretation in terms of nutrient reallocation patterns
journal, February 1995
- Olsson, Stefan
- Mycological Research, Vol. 99, Issue 2
Posttranscriptional Control of Gene Expression in Filamentous Fungi
journal, March 1998
- Sachs, Matthew S.
- Fungal Genetics and Biology, Vol. 23, Issue 2
Quantification of Lignin and Its Structural Features in Plant Biomass Using 13 C Lignin as Internal Standard for Pyrolysis-GC-SIM-MS
journal, October 2017
- van Erven, Gijs; de Visser, Ries; Merkx, Donny W. H.
- Analytical Chemistry, Vol. 89, Issue 20
Fungal enzyme sets for plant polysaccharide degradation
journal, July 2011
- van den Brink, Joost; de Vries, Ronald P.
- Applied Microbiology and Biotechnology, Vol. 91, Issue 6, p. 1477-1492
Localization of growth and secretion of proteins in Aspergillus niger
journal, August 1991
- Wosten, H. A. B.; Moukha, S. M.; Sietsma, J. H.
- Journal of General Microbiology, Vol. 137, Issue 8
Aiming for the complete utilization of sugar-beet pulp: Examination of the effects of mild acid and hydrothermal pretreatment followed by enzymatic digestion
journal, January 2011
- Kühnel, Stefan; Schols, Henk A.; Gruppen, Harry
- Biotechnology for Biofuels, Vol. 4, Issue 1
A Statistical Method for Assessing Peptide Identification Confidence in Accurate Mass and Time Tag Proteomics
journal, August 2011
- Stanley, Jeffrey R.; Adkins, Joshua N.; Slysz, Gordon W.
- Analytical Chemistry, Vol. 83, Issue 16
Expression-based clustering of CAZyme-encoding genes of Aspergillus niger
journal, November 2017
- Gruben, Birgit S.; Mäkelä, Miia R.; Kowalczyk, Joanna E.
- BMC Genomics, Vol. 18, Issue 1
Persistence of adoptively transferred T cells with a kinetically engineered IL-2 receptor agonist
journal, January 2020
- Parisi, Giulia; Saco, Justin D.; Salazar, Felix B.
- Nature Communications, Vol. 11, Issue 1
Spatial differentiation of gene expression in Aspergillus niger colony grown for sugar beet pulp utilization
journal, August 2015
- Benoit, Isabelle; Zhou, Miaomiao; Vivas Duarte, Alexandra
- Scientific Reports, Vol. 5, Issue 1
Simplified method for the measurement of total non-starch polysaccharides by gas-liquid chromatography of constituent sugars as alditol acetates
journal, January 1984
- Englyst, Hans N.; Cummings, John H.
- The Analyst, Vol. 109, Issue 7
Direct transfer of carbon between plants connected by vesicular–arbuscular mycorrhizal mycelium
journal, January 1984
- Francis, R.; Read, D. J.
- Nature, Vol. 307, Issue 5946
Assessment of the pectin degrading enzyme network of Aspergillus niger by functional genomics
journal, March 2009
- Martens-Uzunova, Elena S.; Schaap, Peter J.
- Fungal Genetics and Biology, Vol. 46, Issue 1
Advances in proteomics data analysis and display using an accurate mass and time tag approach
journal, January 2006
- Zimmer, Jennifer S. D.; Monroe, Matthew E.; Qian, Wei-Jun
- Mass Spectrometry Reviews, Vol. 25, Issue 3
Isolation of Aspergillus niger creA mutants and effects of the mutations on expression of arabinases and L-arabinose catabolic enzymes
journal, September 1997
- Ruijter, G. J. G.; Vanhanen, S. A.; Gielkens, M. M. C.
- Microbiology, Vol. 143, Issue 9
2016 update of the PRIDE database and its related tools
journal, November 2015
- Vizcaíno, Juan Antonio; Csordas, Attila; del-Toro, Noemi
- Nucleic Acids Research, Vol. 44, Issue D1
HTSeq--a Python framework to work with high-throughput sequencing data
journal, September 2014
- Anders, S.; Pyl, P. T.; Huber, W.
- Bioinformatics, Vol. 31, Issue 2
Regulation of Plant Biomass Utilization in Aspergillus
book, January 2014
- Kowalczyk, Joanna E.; Benoit, Isabelle; de Vries, Ronald P.
- Advances in Applied Microbiology
2016 update of the PRIDE database and its related tools
journal, November 2015
- Vizcaíno, Juan Antonio; Csordas, Attila; del-Toro, Noemi
- Nucleic Acids Research, Vol. 44, Issue D1
Expression profiling of pectinolytic genes from Aspergillus niger
journal, September 2002
- de Vries, Ronald P.; Jansen, Jenny; Aguilar, Guillermo
- FEBS Letters, Vol. 530, Issue 1-3
Regulators of plant biomass degradation in ascomycetous fungi
journal, June 2017
- Benocci, Tiziano; Aguilar-Pontes, Maria Victoria; Zhou, Miaomiao
- Biotechnology for Biofuels, Vol. 10, Issue 1
Assessment of the pectin degrading enzyme network of Aspergillus niger by functional genomics
journal, March 2009
- Martens-Uzunova, Elena S.; Schaap, Peter J.
- Fungal Genetics and Biology, Vol. 46, Issue 1
Aspergillus Enzymes Involved in Degradation of Plant Cell Wall Polysaccharides
journal, December 2001
- de Vries, R. P.; Visser, J.
- Microbiology and Molecular Biology Reviews, Vol. 65, Issue 4
Closely related fungi employ diverse enzymatic strategies to degrade plant biomass
journal, August 2015
- Benoit, Isabelle; Culleton, Helena; Zhou, Miaomiao
- Biotechnology for Biofuels, Vol. 8, Issue 1
Coarse-scale population structure of pathogenic Armillaria species in a mixed-conifer forest in the Blue Mountains of northeast Oregon
journal, April 2003
- Ferguson, B. A.; Dreisbach, T. A.; Parks, C. G.
- Canadian Journal of Forest Research, Vol. 33, Issue 4
Investigation of the role of typhoid toxin in acute typhoid fever in a human challenge model
text, January 2019
- Gibani, Malick M.; Jones, Elizabeth; Barton, Amber
- Apollo - University of Cambridge Repository
The gold-standard genome of Aspergillus niger NRRL 3 enables a detailed view of the diversity of sugar catabolism in fungi
journal, September 2018
- Aguilar-Pontes, M. V.; Brandl, J.; McDonnell, E.
- Studies in Mycology, Vol. 91
Cytoplasmic Continuity Revisited: Closure of Septa of the Filamentous Fungus Schizophyllum commune in Response to Environmental Conditions
journal, June 2009
- van Peer, Arend F.; Müller, Wally H.; Boekhout, Teun
- PLoS ONE, Vol. 4, Issue 6
Spectral Probabilities and Generating Functions of Tandem Mass Spectra: A Strike against Decoy Databases
journal, August 2008
- Kim, Sangtae; Gupta, Nitin; Pevzner, Pavel A.
- Journal of Proteome Research, Vol. 7, Issue 8
Hyphal homing, fusion and mycelial interconnectedness
journal, March 2004
- Glass, N.
- Trends in Microbiology, Vol. 12, Issue 3
A New Black Aspergillus Species, A. vadensis, Is a Promising Host for Homologous and Heterologous Protein Production
journal, July 2004
- de Vries, R. P.; Burgers, K.; van de Vondervoort, P. J. I.
- Applied and Environmental Microbiology, Vol. 70, Issue 7
MS-GF+ makes progress towards a universal database search tool for proteomics
journal, October 2014
- Kim, Sangtae; Pevzner, Pavel A.
- Nature Communications, Vol. 5, Issue 1
Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2
journal, December 2014
- Love, Michael I.; Huber, Wolfgang; Anders, Simon
- Genome Biology, Vol. 15, Issue 12
The Mycelium as a Network
journal, June 2017
- Fricker, Mark D.; Heaton, Luke L. M.; Jones, Nick S.
- Microbiology Spectrum, Vol. 5, Issue 3