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Title: Colonies of the fungus Aspergillus niger are highly differentiated to adapt to local carbon source variation

Abstract

Saprobic fungi, such as Aspergillus niger, grow as colonies consisting of a network ofbranching and fusing hyphae that are often considered to be relatively uniform entities inwhich nutrients can freely move through the hyphae. In nature, different parts of a colony areoften exposed to different nutrients. We have investigated, using a multi-omics approach,adaptation of A. niger colonies to spatially separated and compositionally different plantbiomass substrates. This demonstrated a high level of intra-colony differentiation, whichclosely matched the locally available substrate. The part of the colony exposed to pectin-richsugar beet pulp and to xylan-rich wheat bran showed high pectinolytic and high xylanolytictranscript and protein levels, respectively.This study therefore exemplifies the high ability of fungal colonies to differentiate and adaptto local conditions, ensuring efficient use of the available nutrients, rather than maintaining auniform physiology throughout the colony.

Authors:
 [1];  [1];  [2]; ORCiD logo [2];  [2]; ORCiD logo [2];  [3]; ORCiD logo [2];  [2]; ORCiD logo [2]; ORCiD logo [2];  [1];  [4];  [3]; ORCiD logo [2];  [5];  [5]
  1. Universiteit Utrecht
  2. BATTELLE (PACIFIC NW LAB)
  3. Wageningen Universiteit
  4. University of Helsinki, Finland
  5. University of Helsinki
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1604781
Report Number(s):
[PNNL-SA-150305]
Grant/Contract Number:  
[AC05-76RL01830]
Resource Type:
Accepted Manuscript
Journal Name:
Environmental Microbiology
Additional Journal Information:
[ Journal Volume: 22; Journal Issue: 3]
Country of Publication:
United States
Language:
English

Citation Formats

Daly, Paul, Peng, Mao, Mitchell, Hugh D., Kim, Young-Mo, Ansong, Charles K., Brewer, Heather M., De Gijsel, Peter, Lipton, Mary S., Markillie, Lye Meng, Nicora, Carrie D., Orr, Galya, Wiebenga, Ad, Hilden, Kristiina S., Kabel, Mirjam A., Baker, Scott E., Makela, Miia, and De Vries, Ronald P. Colonies of the fungus Aspergillus niger are highly differentiated to adapt to local carbon source variation. United States: N. p., 2020. Web. doi:10.1111/1462-2920.14907.
Daly, Paul, Peng, Mao, Mitchell, Hugh D., Kim, Young-Mo, Ansong, Charles K., Brewer, Heather M., De Gijsel, Peter, Lipton, Mary S., Markillie, Lye Meng, Nicora, Carrie D., Orr, Galya, Wiebenga, Ad, Hilden, Kristiina S., Kabel, Mirjam A., Baker, Scott E., Makela, Miia, & De Vries, Ronald P. Colonies of the fungus Aspergillus niger are highly differentiated to adapt to local carbon source variation. United States. doi:10.1111/1462-2920.14907.
Daly, Paul, Peng, Mao, Mitchell, Hugh D., Kim, Young-Mo, Ansong, Charles K., Brewer, Heather M., De Gijsel, Peter, Lipton, Mary S., Markillie, Lye Meng, Nicora, Carrie D., Orr, Galya, Wiebenga, Ad, Hilden, Kristiina S., Kabel, Mirjam A., Baker, Scott E., Makela, Miia, and De Vries, Ronald P. Mon . "Colonies of the fungus Aspergillus niger are highly differentiated to adapt to local carbon source variation". United States. doi:10.1111/1462-2920.14907.
@article{osti_1604781,
title = {Colonies of the fungus Aspergillus niger are highly differentiated to adapt to local carbon source variation},
author = {Daly, Paul and Peng, Mao and Mitchell, Hugh D. and Kim, Young-Mo and Ansong, Charles K. and Brewer, Heather M. and De Gijsel, Peter and Lipton, Mary S. and Markillie, Lye Meng and Nicora, Carrie D. and Orr, Galya and Wiebenga, Ad and Hilden, Kristiina S. and Kabel, Mirjam A. and Baker, Scott E. and Makela, Miia and De Vries, Ronald P.},
abstractNote = {Saprobic fungi, such as Aspergillus niger, grow as colonies consisting of a network ofbranching and fusing hyphae that are often considered to be relatively uniform entities inwhich nutrients can freely move through the hyphae. In nature, different parts of a colony areoften exposed to different nutrients. We have investigated, using a multi-omics approach,adaptation of A. niger colonies to spatially separated and compositionally different plantbiomass substrates. This demonstrated a high level of intra-colony differentiation, whichclosely matched the locally available substrate. The part of the colony exposed to pectin-richsugar beet pulp and to xylan-rich wheat bran showed high pectinolytic and high xylanolytictranscript and protein levels, respectively.This study therefore exemplifies the high ability of fungal colonies to differentiate and adaptto local conditions, ensuring efficient use of the available nutrients, rather than maintaining auniform physiology throughout the colony.},
doi = {10.1111/1462-2920.14907},
journal = {Environmental Microbiology},
number = [3],
volume = [22],
place = {United States},
year = {2020},
month = {3}
}

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