Lanthanide-Binding Tags for 3D X-ray Imaging of Proteins in Cells at Nanoscale Resolution

Journal Article · 09 January 2020 · Journal of the American Chemical Society

DOI: https://doi.org/10.1021/jacs.9b11571 · OSTI ID:1604321

Victor, Tiffany W. ^[1]; O’Toole, Katherine H. ^[2]; Easthon, Lindsey M. ^[2]; Ge, Mingyuan ^[3]; Smith, Randy J. ^[3]; Huang, Xiaojing ^[3]; Yan, Hanfei ^[3]; Chu, Yong S. ^[3]; Chen, Si ^[4]; Gursoy, Doga ^[5]; Ralle, Martina ^[6]; Imperiali, Barbara  ^[7]; Allen, Karen N.  ^[2]; Miller, Lisa M.  ^[1]

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1. Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source II (NSLS-II); Stony Brook Univ., NY (United States). Dept. of Chemistry
2. Boston Univ., MA (United States)
3. Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source II (NSLS-II)
4. Argonne National Lab. (ANL), Lemont, IL (United States). Advanced Photon Source (APS)
5. Argonne National Lab. (ANL), Lemont, IL (United States). Advanced Photon Source (APS); Northwestern Univ., Evanston, IL (United States)
6. Department of Molecular and Medical Genetics, Oregon Health Science University, Portland, Oregon 97239, United States
7. Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)

We report herein the application of lanthanide-binding tags (LBTs) for two- and three-dimensional X-ray imaging of individual proteins in cells with a sub-15 nm beam. The method combines encoded LBTs, which are tags of minimal size (ca. 15–20 amino acids) affording high-affinity lanthanide ion binding, and X-ray fluorescence microscopy (XFM). This approach enables visualization of LBT-tagged proteins while simultaneously measuring the elemental distribution in cells at a spatial resolution necessary for visualizing cell membranes and eukaryotic subcellular organelles.

• Research Organization: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS); Brookhaven National Laboratory (BNL), Upton, NY (United States). National Synchrotron Light Source II (NSLS-II)
• Sponsoring Organization: National Institutes of Health (NIH); National Science Foundation (NSF); USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
• Grant/Contract Number: AC02-06CH11357; SC0012704
• OSTI ID: 1604321
• Report Number(s): BNL--213711-2020-JAAM
• Journal Information: Journal of the American Chemical Society, Journal Name: Journal of the American Chemical Society Journal Issue: 5 Vol. 142; ISSN 0002-7863
• Publisher: American Chemical Society (ACS)Copyright Statement
• Country of Publication: United States
• Language: English