skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Engineering of Bioenergy Crops: Dominant Genetic Approaches to Improve Polysaccharide Properties and Composition in Biomass

Publication Date:
Sponsoring Org.:
OSTI Identifier:
Resource Type:
Published Article
Journal Name:
Frontiers in Plant Science
Additional Journal Information:
[Journal Name: Frontiers in Plant Science Journal Volume: 11]; Journal ID: ISSN 1664-462X
Frontiers Media SA
Country of Publication:

Citation Formats

Brandon, Andrew G., and Scheller, Henrik V. Engineering of Bioenergy Crops: Dominant Genetic Approaches to Improve Polysaccharide Properties and Composition in Biomass. Switzerland: N. p., 2020. Web. doi:10.3389/fpls.2020.00282.
Brandon, Andrew G., & Scheller, Henrik V. Engineering of Bioenergy Crops: Dominant Genetic Approaches to Improve Polysaccharide Properties and Composition in Biomass. Switzerland. doi:10.3389/fpls.2020.00282.
Brandon, Andrew G., and Scheller, Henrik V. Wed . "Engineering of Bioenergy Crops: Dominant Genetic Approaches to Improve Polysaccharide Properties and Composition in Biomass". Switzerland. doi:10.3389/fpls.2020.00282.
title = {Engineering of Bioenergy Crops: Dominant Genetic Approaches to Improve Polysaccharide Properties and Composition in Biomass},
author = {Brandon, Andrew G. and Scheller, Henrik V.},
abstractNote = {},
doi = {10.3389/fpls.2020.00282},
journal = {Frontiers in Plant Science},
number = ,
volume = [11],
place = {Switzerland},
year = {2020},
month = {3}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.3389/fpls.2020.00282

Save / Share:

Works referenced in this record:

Expression of fungal acetyl xylan esterase in Arabidopsis thaliana improves saccharification of stem lignocellulose
journal, May 2015

  • Pawar, Prashant Mohan-Anupama; Derba-Maceluch, Marta; Chong, Sun-Li
  • Plant Biotechnology Journal, Vol. 14, Issue 1
  • DOI: 10.1111/pbi.12393

Cell wall modification in tobacco by differential targeting of recombinant endoglucanase from Trichoderma reesei
journal, January 2015

Sugar release and growth of biofuel crops are improved by downregulation of pectin biosynthesis
journal, February 2018

  • Biswal, Ajaya K.; Atmodjo, Melani A.; Li, Mi
  • Nature Biotechnology, Vol. 36, Issue 3
  • DOI: 10.1038/nbt.4067

Increase in Cellulose Accumulation and Improvement of Saccharification by Overexpression of Arabinofuranosidase in Rice
journal, November 2013

Powerful regulatory systems and post-transcriptional gene silencing resist increases in cellulose content in cell walls of barley
journal, January 2015

Enhancement of growth and cellulose accumulation by overexpression of xyloglucanase in poplar
journal, March 2004

Perennial Forages as Second Generation Bioenergy Crops
journal, May 2008

  • Sanderson, Matt; Adler, Paul
  • International Journal of Molecular Sciences, Vol. 9, Issue 5
  • DOI: 10.3390/ijms9050768

Growth of the plant cell wall
journal, November 2005

  • Cosgrove, Daniel J.
  • Nature Reviews Molecular Cell Biology, Vol. 6, Issue 11, p. 850-861
  • DOI: 10.1038/nrm1746

Covalent interactions between lignin and hemicelluloses in plant secondary cell walls
journal, April 2019

An unusual xylan in Arabidopsis primary cell walls is synthesised by GUX3, IRX9L, IRX10L and IRX14
journal, June 2015

  • Mortimer, Jenny C.; Faria-Blanc, Nuno; Yu, Xiaolan
  • The Plant Journal, Vol. 83, Issue 3
  • DOI: 10.1111/tpj.12898

Cellulose crystallinity - a key predictor of the enzymatic hydrolysis rate: Cellulose crystallinity
journal, February 2010

Perturbation of Wood Cellulose Synthesis Causes Pleiotropic Effects in Transgenic Aspen
journal, March 2011

  • Joshi, Chandrashekhar P.; Thammannagowda, Shivegowda; Fujino, Takeshi
  • Molecular Plant, Vol. 4, Issue 2
  • DOI: 10.1093/mp/ssq081

The Carbohydrate-Active EnZymes database (CAZy): an expert resource for Glycogenomics
journal, January 2009

  • Cantarel, B. L.; Coutinho, P. M.; Rancurel, C.
  • Nucleic Acids Research, Vol. 37, Issue Database
  • DOI: 10.1093/nar/gkn663

Comparison of five xylan synthesis mutants reveals new insight into the mechanisms of xylan synthesis
journal, December 2007

Recombinant hyperthermophilic enzyme expression in plants: a novel approach for lignocellulose digestion
journal, May 2014

Three AtCesA6-like members enhance biomass production by distinctively promoting cell growth in Arabidopsis
journal, October 2017

  • Hu, Huizhen; Zhang, Ran; Feng, Shengqiu
  • Plant Biotechnology Journal, Vol. 16, Issue 5
  • DOI: 10.1111/pbi.12842

Engineering temporal accumulation of a low recalcitrance polysaccharide leads to increased C6 sugar content in plant cell walls
journal, January 2015

  • Vega-Sánchez, Miguel E.; Loqué, Dominique; Lao, Jeemeng
  • Plant Biotechnology Journal, Vol. 13, Issue 7
  • DOI: 10.1111/pbi.12326

The targeting of starch binding domains from starch synthase III to the cell wall alters cell wall composition and properties
journal, October 2016

  • Grisolia, Mauricio J.; Peralta, Diego A.; Valdez, Hugo A.
  • Plant Molecular Biology, Vol. 93, Issue 1-2
  • DOI: 10.1007/s11103-016-0551-y

Loosening Xyloglucan Accelerates the Enzymatic Degradation of Cellulose in Wood
journal, September 2009

  • Kaida, Rumi; Kaku, Tomomi; Baba, Kei'ichi
  • Molecular Plant, Vol. 2, Issue 5
  • DOI: 10.1093/mp/ssp060

Supply and demand: sink regulation of sugar accumulation in sugarcane
journal, January 2009

  • McCormick, A. J.; Watt, D. A.; Cramer, M. D.
  • Journal of Experimental Botany, Vol. 60, Issue 2
  • DOI: 10.1093/jxb/ern310

Land Availability for Biofuel Production
journal, January 2011

  • Cai, Ximing; Zhang, Xiao; Wang, Dingbao
  • Environmental Science & Technology, Vol. 45, Issue 1
  • DOI: 10.1021/es103338e

Expression of a fungal ferulic acid esterase increases cell wall digestibility of tall fescue (Festuca arundinacea)
journal, April 2008

Feruloylation in Grasses: Current and Future Perspectives
journal, September 2009

PMR 5, an acetylation protein at the intersection of pectin biosynthesis and defense against fungal pathogens
journal, October 2019

  • Chiniquy, Dawn; Underwood, William; Corwin, Jason
  • The Plant Journal, Vol. 100, Issue 5
  • DOI: 10.1111/tpj.14497

A β-glucuronosyltransferase from Arabidopsis thaliana involved in biosynthesis of type II arabinogalactan has a role in cell elongation during seedling growth
journal, November 2013

  • Knoch, Eva; Dilokpimol, Adiphol; Tryfona, Theodora
  • The Plant Journal, Vol. 76, Issue 6
  • DOI: 10.1111/tpj.12353

Decreased Polysaccharide Feruloylation Compromises Plant Cell Wall Integrity and Increases Susceptibility to Necrotrophic Fungal Pathogens
journal, May 2016

  • Reem, Nathan T.; Pogorelko, Gennady; Lionetti, Vincenzo
  • Frontiers in Plant Science, Vol. 7
  • DOI: 10.3389/fpls.2016.00630

Overexpression of Poplar Xylem Sucrose Synthase in Tobacco Leads to a Thickened Cell Wall and Increased Height
journal, March 2015

Engineering Non-cellulosic Polysaccharides of Wood for the Biorefinery
journal, October 2018

  • Donev, Evgeniy; Gandla, Madhavi Latha; Jönsson, Leif J.
  • Frontiers in Plant Science, Vol. 9
  • DOI: 10.3389/fpls.2018.01537

Cellulose Metabolism in Plants
journal, January 2005

Genetic modification of plant cell walls to enhance biomass yield and biofuel production in bioenergy crops
journal, September 2016

Plant Glycosyltransferases Beyond CAZy: A Perspective on DUF Families
journal, January 2012

Characterizing the Link between Glycosylation State and Enzymatic Activity of the Endo-β1,4-glucanase KORRIGAN1 from Arabidopsis thaliana
journal, June 2013

  • Liebminger, Eva; Grass, Josephine; Altmann, Friedrich
  • Journal of Biological Chemistry, Vol. 288, Issue 31
  • DOI: 10.1074/jbc.M113.475558

The PARVUS Gene is Expressed in Cells Undergoing Secondary Wall Thickening and is Essential for Glucuronoxylan Biosynthesis
journal, December 2007

  • Lee, Chanhui; Zhong, Ruiqin; Richardson, Elizabeth A.
  • Plant and Cell Physiology, Vol. 48, Issue 12
  • DOI: 10.1093/pcp/pcm155

Characterization of IRX10 and IRX10-like reveals an essential role in glucuronoxylan biosynthesis in Arabidopsis
journal, February 2009

Down-Regulation of KORRIGAN-Like Endo-β-1,4-Glucanase Genes Impacts Carbon Partitioning, Mycorrhizal Colonization and Biomass Production in Populus
journal, October 2016

  • Kalluri, Udaya C.; Payyavula, Raja S.; Labbé, Jessy L.
  • Frontiers in Plant Science, Vol. 7
  • DOI: 10.3389/fpls.2016.01455

Carbon partitioning in sugarcane (Saccharum species)
journal, January 2013

Transgenic switchgrass ( Panicum virgatum L.) biomass is increased by overexpression of switchgrass sucrose synthase ( PvSUS1 )
journal, November 2014

  • Poovaiah, Charleson R.; Mazarei, Mitra; Decker, Stephen R.
  • Biotechnology Journal, Vol. 10, Issue 4
  • DOI: 10.1002/biot.201400499

Xylan O-Acetylation Impacts Xylem Development and Enzymatic Recalcitrance as Indicated by the Arabidopsis Mutant tbl29
journal, July 2013

  • Xiong, Guangyan; Cheng, Kun; Pauly, Markus
  • Molecular Plant, Vol. 6, Issue 4
  • DOI: 10.1093/mp/sst014

Arabidopsis thaliana IRX10 and two related proteins from psyllium and Physcomitrella patens are xylan xylosyltransferases
journal, September 2014

  • Jensen, Jacob Krüger; Johnson, Nathan Robert; Wilkerson, Curtis Gene
  • The Plant Journal, Vol. 80, Issue 2
  • DOI: 10.1111/tpj.12641

Two Arabidopsis proteins synthesize acetylated xylan in vitro
journal, September 2014

  • Urbanowicz, Breeanna R.; Peña, Maria J.; Moniz, Heather A.
  • The Plant Journal, Vol. 80, Issue 2
  • DOI: 10.1111/tpj.12643

Strategies for the production of cell wall-deconstructing enzymes in lignocellulosic biomass and their utilization for biofuel production
journal, December 2015

  • Park, Sang-Hyuck; Ong, Rebecca Garlock; Sticklen, Mariam
  • Plant Biotechnology Journal, Vol. 14, Issue 6
  • DOI: 10.1111/pbi.12505

Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification
journal, September 2018

Arabidopsis Family GT43 Members are Xylan Xylosyltransferases Required for the Elongation of the Xylan Backbone
journal, November 2011

  • Lee, C.; Zhong, R.; Ye, Z. -H.
  • Plant and Cell Physiology, Vol. 53, Issue 1
  • DOI: 10.1093/pcp/pcr158

Downregulation of a UDP-Arabinomutase Gene in Switchgrass (Panicum virgatum L.) Results in Increased Cell Wall Lignin While Reducing Arabinose-Glycans
journal, October 2016

  • Willis, Jonathan D.; Smith, James A.; Mazarei, Mitra
  • Frontiers in Plant Science, Vol. 7
  • DOI: 10.3389/fpls.2016.01580

Regulation of (1,3;1,4)-β-d-glucan synthesis in developing endosperm of barley lys mutants
journal, January 2012

The Cell Biology of Cellulose Synthesis
journal, April 2014

Feedstocks for Lignocellulosic Biofuels
journal, August 2010

  • Somerville, Cris; Youngs, Heather; Taylor, Caroline
  • Science, Vol. 329, Issue 5993, p. 790-792
  • DOI: 10.1126/science.1189268

journal, June 2010

Xylan biosynthesis
journal, April 2014

Targeted Modification of Homogalacturonan by Transgenic Expression of a Fungal Polygalacturonase Alters Plant Growth
journal, July 2004

  • Capodicasa, Cristina; Vairo, Donatella; Zabotina, Olga
  • Plant Physiology, Vol. 135, Issue 3
  • DOI: 10.1104/pp.104.042788

Lignocellulose conversion to biofuels: current challenges, global perspectives
journal, June 2009

Sucrose synthase affects carbon partitioning to increase cellulose production and altered cell wall ultrastructure
journal, July 2009

  • Coleman, H. D.; Yan, J.; Mansfield, S. D.
  • Proceedings of the National Academy of Sciences, Vol. 106, Issue 31
  • DOI: 10.1073/pnas.0900188106

Exploiting the potential of plants with crassulacean acid metabolism for bioenergy production on marginal lands
journal, April 2009

  • Borland, A. M.; Griffiths, H.; Hartwell, J.
  • Journal of Experimental Botany, Vol. 60, Issue 10
  • DOI: 10.1093/jxb/erp118

Expression of a fungal ferulic acid esterase in suspension cultures of tall fescue (Festuca arundinacea) decreases cell wall feruloylation and increases rates of cell wall digestion
journal, February 2017

  • Morris, Phillip; Dalton, Sue; Langdon, Tim
  • Plant Cell, Tissue and Organ Culture (PCTOC), Vol. 129, Issue 2
  • DOI: 10.1007/s11240-017-1168-9

The plant glycosyltransferase clone collection for functional genomics
journal, July 2014

  • Lao, Jeemeng; Oikawa, Ai; Bromley, Jennifer R.
  • The Plant Journal, Vol. 79, Issue 3
  • DOI: 10.1111/tpj.12577

Growth modulation of transgenic potato plants by heterologous expression of bacterial carbohydrate-binding module
journal, May 2006

An Overview of Sucrose Synthases in Plants
journal, February 2019

The cell biology of secondary cell wall biosynthesis
journal, February 2018

  • Meents, Miranda J.; Watanabe, Yoichiro; Samuels, A. Lacey
  • Annals of Botany, Vol. 121, Issue 6
  • DOI: 10.1093/aob/mcy005

Hemicelluloses for fuel ethanol: A review
journal, July 2010

Arabidopsis irregular xylem8 and irregular xylem9 : Implications for the Complexity of Glucuronoxylan Biosynthesis
journal, February 2007

Gene stacking of multiple traits for high yield of fermentable sugars in plant biomass
journal, January 2018

  • Aznar, Aude; Chalvin, Camille; Shih, Patrick M.
  • Biotechnology for Biofuels, Vol. 11, Issue 1
  • DOI: 10.1186/s13068-017-1007-6

Engineering of plants with improved properties as biofuels feedstocks by vessel-specific complementation of xylan biosynthesis mutants
journal, January 2012

  • Petersen, Pia Damm; Lau, Jane; Ebert, Berit
  • Biotechnology for Biofuels, Vol. 5, Issue 1, Article No. 84
  • DOI: 10.1186/1754-6834-5-84

Improving the utilization of lignocellulosic biomass by polysaccharide modification
journal, February 2016

Engineering of plant cell walls for enhanced biofuel production
journal, June 2015

  • Loqué, Dominique; Scheller, Henrik V.; Pauly, Markus
  • Current Opinion in Plant Biology, Vol. 25
  • DOI: 10.1016/j.pbi.2015.05.018

Growth modulation effects of CBM2a under the control of AtEXP4 and CaMV35S promoters in Arabidopsis thaliana, Nicotiana tabacum and Eucalyptus camaldulensis
journal, March 2017

  • Keadtidumrongkul, Pornthep; Suttangkakul, Anongpat; Pinmanee, Phitsanu
  • Transgenic Research, Vol. 26, Issue 4
  • DOI: 10.1007/s11248-017-0015-4

Sustainable use of eucalypt biomass grown on short rotation coppice for bioenergy
journal, July 2016

  • Eufrade Junior, Humbertode Jesus; Melo, Raoni Xavier de; Sartori, Maria Márcia Pereira
  • Biomass and Bioenergy, Vol. 90
  • DOI: 10.1016/j.biombioe.2016.03.037

Expression of a fungal ferulic acid esterase in alfalfa modifies cell wall digestibility
journal, January 2014

Meeting US biofuel goals with less land: the potential of Miscanthus
journal, September 2008

Loosening of plant cell walls by expansins
journal, September 2000

Genetic engineering of grass cell wall polysaccharides for biorefining
journal, June 2017

  • Bhatia, Rakesh; Gallagher, Joe A.; Gomez, Leonardo D.
  • Plant Biotechnology Journal, Vol. 15, Issue 9
  • DOI: 10.1111/pbi.12764

Inactivation of OsIRX10 Leads to Decreased Xylan Content in Rice Culm Cell Walls and Improved Biomass Saccharification
journal, March 2013

  • Chen, Xuewei; Vega-Sánchez, Miguel E.; Verhertbruggen, Yves
  • Molecular Plant, Vol. 6, Issue 2
  • DOI: 10.1093/mp/sss135

Plant cell wall reconstruction toward improved lignocellulosic production and processability
journal, February 2010

Engineering the cell wall by reducing de-methyl-esterified homogalacturonan improves saccharification of plant tissues for bioconversion
journal, December 2009

  • Lionetti, V.; Francocci, F.; Ferrari, S.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 2
  • DOI: 10.1073/pnas.0907549107

Post-synthetic modification of plant cell walls by expression of microbial hydrolases in the apoplast
journal, September 2011

Identification of Novel Genes in Arabidopsis Involved in Secondary Cell Wall Formation Using Expression Profiling and Reverse Genetics
journal, June 2005

  • Brown, David M.; Zeef, Leo A. H.; Ellis, Joanne
  • The Plant Cell, Vol. 17, Issue 8
  • DOI: 10.1105/tpc.105.031542

A functional cellulose synthase from ascidian epidermis
journal, January 2004

  • Matthysse, A. G.; Deschet, K.; Williams, M.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 4
  • DOI: 10.1073/pnas.0303623101

In planta expression of A. cellulolyticus Cel5A endocellulase reduces cell wall recalcitrance in tobacco and maize
journal, January 2011

  • Brunecky, Roman; Selig, Michael J.; Vinzant, Todd B.
  • Biotechnology for Biofuels, Vol. 4, Issue 1
  • DOI: 10.1186/1754-6834-4-1

Overexpression of a BAHD Acyltransferase, OsAt10 , Alters Rice Cell Wall Hydroxycinnamic Acid Content and Saccharification
journal, February 2013

  • Bartley, Laura E.; Peck, Matthew L.; Kim, Sung-Ryul
  • Plant Physiology, Vol. 161, Issue 4
  • DOI: 10.1104/pp.112.208694

Promiscuous, non-catalytic, tandem carbohydrate-binding modules modulate the cell-wall structure and development of transgenic tobacco (Nicotiana tabacum) plants
journal, July 2007

  • Obembe, Olawole O.; Jacobsen, Evert; Timmers, Jaap
  • Journal of Plant Research, Vol. 120, Issue 5
  • DOI: 10.1007/s10265-007-0099-7

Evolving Views of Pectin Biosynthesis
journal, April 2013

Overexpression of a Domain of Unknown Function 231-containing protein increases O-xylan acetylation and cellulose biosynthesis in Populus
journal, December 2017

  • Yang, Yongil; Yoo, Chang Geun; Winkeler, Kimberly A.
  • Biotechnology for Biofuels, Vol. 10, Issue 1
  • DOI: 10.1186/s13068-017-0998-3

Downregulation of pectin biosynthesis gene GAUT4 leads to reduced ferulate and lignin-carbohydrate cross-linking in switchgrass
journal, January 2019

Controlled expression of pectic enzymes in Arabidopsis thaliana enhances biomass conversion without adverse effects on growth
journal, April 2015

A dominant negative approach to reduce xylan in plants
journal, June 2019

  • Brandon, Andrew G.; Birdseye, Devon S.; Scheller, Henrik V.
  • Plant Biotechnology Journal, Vol. 18, Issue 1
  • DOI: 10.1111/pbi.13198

Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase
journal, June 2017

In muro deacetylation of xylan affects lignin properties and improves saccharification of aspen wood
journal, April 2017

  • Pawar, Prashant Mohan-Anupama; Derba-Maceluch, Marta; Chong, Sun-Li
  • Biotechnology for Biofuels, Vol. 10, Issue 1
  • DOI: 10.1186/s13068-017-0782-4

Sucrose Synthase is an Integral Component of the Cellulose Synthesis Machinery
journal, January 2010

  • Fujii, S.; Hayashi, T.; Mizuno, K.
  • Plant and Cell Physiology, Vol. 51, Issue 2
  • DOI: 10.1093/pcp/pcp190

Brittle Culm1, a COBRA-Like Protein, Functions in Cellulose Assembly through Binding Cellulose Microfibrils
journal, August 2013

Aspen pectate lyase PtxtPL1-27 mobilizes matrix polysaccharides from woody tissues and improves saccharification yield
journal, January 2014

  • Biswal, Ajaya K.; Soeno, Kazuo; Gandla, Madhavi
  • Biotechnology for Biofuels, Vol. 7, Issue 1
  • DOI: 10.1186/1754-6834-7-11

A gene stacking approach leads to engineered plants with highly increased galactan levels in Arabidopsis
journal, December 2014

Genetic and molecular basis of grass cell-wall degradability. I. Lignin–cell wall matrix interactions
journal, May 2004