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Title: Detection of Abrin-Like and Prepropulchellin-Like Toxin Genes and Transcripts Using Whole Genome Sequencing and Full-Length Transcript Sequencing of Abrus precatorius

Abstract

The sequenced genome and the leaf transcriptome of a near relative of Abrus pulchellus and Abrus precatorius was analyzed to characterize the genetic basis of toxin gene expression. From the high-quality genome assembly, a total of 26 potential coding regions were identified that contain genes with abrin-like, pulchellin-like, and agglutinin-like homology, with full-length transcripts detected in leaf tissue for 9 of the 26 coding regions. All of the toxin-like genes were identified within only five isolated regions of the genome, with each region containing 1 to 16 gene variants within each genomic region (<1 Mbp). The Abrus precatorius cultivar sequenced here contains genes which encode for proteins that are homologous to certain abrin and prepropulchellin genes previously identified, and we observed substantial diversity of genes and predicted gene products in Abrus precatorius and previously characterized toxins. This suggests diverse toxin repertoires within Abrus, potentially the results of rapid toxin evolution.

Authors:
ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1];  [1];  [2]; ORCiD logo [1]; ORCiD logo [1]
  1. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
  2. Univ. of Arizona, Tucson, AZ (United States)
Publication Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
US Department of Homeland Security (DHS)
OSTI Identifier:
1603997
Report Number(s):
LA-UR-19-27275
Journal ID: ISSN 2072-6651; TOXIB7
Grant/Contract Number:  
89233218CNA000001; HSHQPM-16-X-00214
Resource Type:
Accepted Manuscript
Journal Name:
Toxins
Additional Journal Information:
Journal Volume: 11; Journal Issue: 12; Journal ID: ISSN 2072-6651
Publisher:
MDPI
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; abrin; pulchellin; full genome sequencing; genome assembly; isoform; Iso-Seq; Abrus precatorius; toxin evolution

Citation Formats

Hovde, Blake T., Daligault, Hajnalka Erzsebet, Hanschen, Erik Richard, Kunde, Yuliya A., Johnson, Matthew B., Starkenburg, Shawn Robert, and Johnson, Shannon Lyn. Detection of Abrin-Like and Prepropulchellin-Like Toxin Genes and Transcripts Using Whole Genome Sequencing and Full-Length Transcript Sequencing of Abrus precatorius. United States: N. p., 2019. Web. doi:10.3390/toxins11120691.
Hovde, Blake T., Daligault, Hajnalka Erzsebet, Hanschen, Erik Richard, Kunde, Yuliya A., Johnson, Matthew B., Starkenburg, Shawn Robert, & Johnson, Shannon Lyn. Detection of Abrin-Like and Prepropulchellin-Like Toxin Genes and Transcripts Using Whole Genome Sequencing and Full-Length Transcript Sequencing of Abrus precatorius. United States. doi:https://doi.org/10.3390/toxins11120691
Hovde, Blake T., Daligault, Hajnalka Erzsebet, Hanschen, Erik Richard, Kunde, Yuliya A., Johnson, Matthew B., Starkenburg, Shawn Robert, and Johnson, Shannon Lyn. Mon . "Detection of Abrin-Like and Prepropulchellin-Like Toxin Genes and Transcripts Using Whole Genome Sequencing and Full-Length Transcript Sequencing of Abrus precatorius". United States. doi:https://doi.org/10.3390/toxins11120691. https://www.osti.gov/servlets/purl/1603997.
@article{osti_1603997,
title = {Detection of Abrin-Like and Prepropulchellin-Like Toxin Genes and Transcripts Using Whole Genome Sequencing and Full-Length Transcript Sequencing of Abrus precatorius},
author = {Hovde, Blake T. and Daligault, Hajnalka Erzsebet and Hanschen, Erik Richard and Kunde, Yuliya A. and Johnson, Matthew B. and Starkenburg, Shawn Robert and Johnson, Shannon Lyn},
abstractNote = {The sequenced genome and the leaf transcriptome of a near relative of Abrus pulchellus and Abrus precatorius was analyzed to characterize the genetic basis of toxin gene expression. From the high-quality genome assembly, a total of 26 potential coding regions were identified that contain genes with abrin-like, pulchellin-like, and agglutinin-like homology, with full-length transcripts detected in leaf tissue for 9 of the 26 coding regions. All of the toxin-like genes were identified within only five isolated regions of the genome, with each region containing 1 to 16 gene variants within each genomic region (<1 Mbp). The Abrus precatorius cultivar sequenced here contains genes which encode for proteins that are homologous to certain abrin and prepropulchellin genes previously identified, and we observed substantial diversity of genes and predicted gene products in Abrus precatorius and previously characterized toxins. This suggests diverse toxin repertoires within Abrus, potentially the results of rapid toxin evolution.},
doi = {10.3390/toxins11120691},
journal = {Toxins},
number = 12,
volume = 11,
place = {United States},
year = {2019},
month = {11}
}

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