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Title: Microbial Diversity in Bushmeat Samples Recovered from the Serengeti Ecosystem in Tanzania

Abstract

Bushmeat, the meat and organs derived from wildlife species, is a common source of animal protein in the diets of those living in sub-Saharan Africa and is frequently associated with zoonotic spillover of dangerous pathogens. Given the frequent consumption of bushmeat in this region and the lack of knowledge about the microbial communities associated with this meat, the microbiome of 56 fresh and processed bushmeat samples ascertained from three districts in the Western Serengeti ecosystem in Tanzania was characterized using 16S rRNA metagenomic sequencing. Here, the results show that the most abundant phyla present in bushmeat samples include Firmicutes (67.8%), Proteobacteria (18.4%), Cyanobacteria (8.9%), and Bacteroidetes (3.1%). Regardless of wildlife species, sample condition, season, or region, the microbiome is diverse across all samples, with no significant difference in alpha or beta diversity. The findings also suggest the presence of DNA signatures of potentially dangerous zoonotic pathogens, including those from the genus Bacillus, Brucella, Coxiella, and others, in bushmeat. Together, this investigation provides a better understanding of the microbiome associated with this major food source in samples collected from the Western Serengeti in Tanzania and highlights a need for future investigations on the potential health risks associated with the harvesting, trade,more » and consumption of bushmeat in Sub-Saharan Africa.« less

Authors:
ORCiD logo [1];  [1]; ORCiD logo [2];  [3]; ORCiD logo [1];  [4];  [2];  [5];  [1];  [6]; ORCiD logo [7]; ORCiD logo [7];  [2];  [2];  [8];  [3];  [1];  [9];  [1];  [2] more »;  [5] « less
  1. Pennsylvania State Univ., University Park, PA (United States)
  2. Nelson Mandela African Inst. of Science and Technology, Arusha (Tanzania)
  3. Biosciences eastern and central Africa-International Livestock Research Inst. (BecA-ILRI) Hub, Nairobi (Kenya)
  4. Nelson Mandela African Inst. of Science and Technology, Arusha (Tanzania); Tanzania Wildlife Research Inst., Arusha (Tanzania)
  5. Pennsylvania State Univ., University Park, PA (United States); Nelson Mandela African Inst. of Science and Technology, Arusha (Tanzania)
  6. Lincoln Park Zoo, Chicago, IL (United States)
  7. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
  8. European Molecular Biology Laboratory (EMBL), Heidelberg (Germany); Biosciences eastern and central Africa-International Livestock Research Inst. (BecA-ILRI) Hub, Nairobi (Kenya)
  9. Ministry of Health Community Development Gender Elderly and Children, Dar es Salaam (Tanzania)
Publication Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
U.S. Department of Defense, Defense Threat Reduction Agency, Biological Threat Reduction Program; USDA National Inst. of Food and Agriculture and Multistate
OSTI Identifier:
1603985
Report Number(s):
LA-UR-19-20024
Journal ID: ISSN 2045-2322
Grant/Contract Number:  
89233218CNA000001; HDTRA1-16- 1-0005; PEN04637; 1014673
Resource Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
Biological Science

Citation Formats

Katani, Robab, Schilling, Megan A., Lyimo, Beatus, Tonui, Triza, Cattadori, Isabella M., Eblate, Ernest, Martin, Andimile, Estes, Anna B., Buza, Teresia, Rentsch, Dennis, Davenport, Karen Walston, Hovde, Blake T., Lyimo, Samson, Munuo, Lydia, Stomeo, Francesca, Tiambo, Christian, Radzio-Basu, Jessica, Mosha, Fausta, Hudson, Peter J., Buza, Joram J., and Kapur, Vivek. Microbial Diversity in Bushmeat Samples Recovered from the Serengeti Ecosystem in Tanzania. United States: N. p., 2019. Web. doi:10.1038/s41598-019-53969-7.
Katani, Robab, Schilling, Megan A., Lyimo, Beatus, Tonui, Triza, Cattadori, Isabella M., Eblate, Ernest, Martin, Andimile, Estes, Anna B., Buza, Teresia, Rentsch, Dennis, Davenport, Karen Walston, Hovde, Blake T., Lyimo, Samson, Munuo, Lydia, Stomeo, Francesca, Tiambo, Christian, Radzio-Basu, Jessica, Mosha, Fausta, Hudson, Peter J., Buza, Joram J., & Kapur, Vivek. Microbial Diversity in Bushmeat Samples Recovered from the Serengeti Ecosystem in Tanzania. United States. doi:10.1038/s41598-019-53969-7.
Katani, Robab, Schilling, Megan A., Lyimo, Beatus, Tonui, Triza, Cattadori, Isabella M., Eblate, Ernest, Martin, Andimile, Estes, Anna B., Buza, Teresia, Rentsch, Dennis, Davenport, Karen Walston, Hovde, Blake T., Lyimo, Samson, Munuo, Lydia, Stomeo, Francesca, Tiambo, Christian, Radzio-Basu, Jessica, Mosha, Fausta, Hudson, Peter J., Buza, Joram J., and Kapur, Vivek. Mon . "Microbial Diversity in Bushmeat Samples Recovered from the Serengeti Ecosystem in Tanzania". United States. doi:10.1038/s41598-019-53969-7. https://www.osti.gov/servlets/purl/1603985.
@article{osti_1603985,
title = {Microbial Diversity in Bushmeat Samples Recovered from the Serengeti Ecosystem in Tanzania},
author = {Katani, Robab and Schilling, Megan A. and Lyimo, Beatus and Tonui, Triza and Cattadori, Isabella M. and Eblate, Ernest and Martin, Andimile and Estes, Anna B. and Buza, Teresia and Rentsch, Dennis and Davenport, Karen Walston and Hovde, Blake T. and Lyimo, Samson and Munuo, Lydia and Stomeo, Francesca and Tiambo, Christian and Radzio-Basu, Jessica and Mosha, Fausta and Hudson, Peter J. and Buza, Joram J. and Kapur, Vivek},
abstractNote = {Bushmeat, the meat and organs derived from wildlife species, is a common source of animal protein in the diets of those living in sub-Saharan Africa and is frequently associated with zoonotic spillover of dangerous pathogens. Given the frequent consumption of bushmeat in this region and the lack of knowledge about the microbial communities associated with this meat, the microbiome of 56 fresh and processed bushmeat samples ascertained from three districts in the Western Serengeti ecosystem in Tanzania was characterized using 16S rRNA metagenomic sequencing. Here, the results show that the most abundant phyla present in bushmeat samples include Firmicutes (67.8%), Proteobacteria (18.4%), Cyanobacteria (8.9%), and Bacteroidetes (3.1%). Regardless of wildlife species, sample condition, season, or region, the microbiome is diverse across all samples, with no significant difference in alpha or beta diversity. The findings also suggest the presence of DNA signatures of potentially dangerous zoonotic pathogens, including those from the genus Bacillus, Brucella, Coxiella, and others, in bushmeat. Together, this investigation provides a better understanding of the microbiome associated with this major food source in samples collected from the Western Serengeti in Tanzania and highlights a need for future investigations on the potential health risks associated with the harvesting, trade, and consumption of bushmeat in Sub-Saharan Africa.},
doi = {10.1038/s41598-019-53969-7},
journal = {Scientific Reports},
number = 1,
volume = 9,
place = {United States},
year = {2019},
month = {12}
}

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Works referenced in this record:

Wild meat: the bigger picture
journal, July 2003


Role of Gut Microbiota in Rheumatoid Arthritis
journal, June 2017

  • Maeda, Yuichi; Takeda, Kiyoshi
  • Journal of Clinical Medicine, Vol. 6, Issue 6
  • DOI: 10.3390/jcm6060060

Clostridiaceae and Enterobacteriaceae as active fermenters in earthworm gut content
journal, July 2010

  • Wüst, Pia K.; Horn, Marcus A.; Drake, Harold L.
  • The ISME Journal, Vol. 5, Issue 1
  • DOI: 10.1038/ismej.2010.99

Pyrosequencing-based analysis of fecal microbial communities in three purebred pig lines
journal, July 2014

  • Pajarillo, Edward Alain B.; Chae, Jong Pyo; Balolong, Marilen P.
  • Journal of Microbiology, Vol. 52, Issue 8
  • DOI: 10.1007/s12275-014-4270-2

Hunting and trading bushmeat in the Kilombero Valley, Tanzania: motivations, cost-benefit ratios and meat prices
journal, July 2014


Pvclust: an R package for assessing the uncertainty in hierarchical clustering
journal, April 2006


Movements of the migratory wildebeest population in the Serengeti area between 1960 and 1973
journal, March 1975


Bushmeat Hunting, Deforestation, and Prediction of Zoonotic Disease
journal, December 2005

  • Wolfe, Nathan D.; Daszak, Peter; Kilpatrick, A. Marm
  • Emerging Infectious Diseases, Vol. 11, Issue 12
  • DOI: 10.3201/eid1112.040789

A diversity profile of the human skin microbiota
journal, July 2008


The Bushmeat Trade: Increased Opportunities for Transmission of Zoonotic Disease
journal, October 2009

  • Karesh, William B.; Noble, Eric
  • Mount Sinai Journal of Medicine: A Journal of Translational and Personalized Medicine, Vol. 76, Issue 5
  • DOI: 10.1002/msj.20139

The socio-economic drivers of bushmeat consumption during the West African Ebola crisis
journal, March 2017

  • Ordaz-Németh, Isabel; Arandjelovic, Mimi; Boesch, Lukas
  • PLOS Neglected Tropical Diseases, Vol. 11, Issue 3
  • DOI: 10.1371/journal.pntd.0005450

QIIME allows analysis of high-throughput community sequencing data
journal, April 2010

  • Caporaso, J. Gregory; Kuczynski, Justin; Stombaugh, Jesse
  • Nature Methods, Vol. 7, Issue 5
  • DOI: 10.1038/nmeth.f.303

Waste Not, Want Not: Why Rarefying Microbiome Data Is Inadmissible
journal, April 2014


Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample
journal, June 2010

  • Caporaso, J. G.; Lauber, C. L.; Walters, W. A.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue Supplement_1
  • DOI: 10.1073/pnas.1000080107

The bushmeat trade in African savannas: Impacts, drivers, and possible solutions
journal, April 2013


The scale of illegal meat importation from Africa to Europe via Paris: Illegal intercontinental meat trade
journal, June 2010


Characterising Wildlife Trade Market Supply-Demand Dynamics
journal, September 2016


Bushmeat Hunting, Wildlife Declines, and Fish Supply in West Africa
journal, November 2004


Ecology of a Grazing Ecosystem: The Serengeti
journal, February 1985

  • McNaughton, S. J.
  • Ecological Monographs, Vol. 55, Issue 3
  • DOI: 10.2307/1942578

Population Consequences of Predation-Sensitive Foraging: The Serengeti Wildebeest
journal, April 1995

  • Sinclair, A. R. E.; Arcese, P.
  • Ecology, Vol. 76, Issue 3
  • DOI: 10.2307/1939353

Bushmeat and International Development
journal, June 2002


Clostridium and Bacillus Binary Enterotoxins: Bad for the Bowels, and Eukaryotic Being
journal, September 2014


Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms
journal, March 2012

  • Caporaso, J. Gregory; Lauber, Christian L.; Walters, William A.
  • The ISME Journal, Vol. 6, Issue 8
  • DOI: 10.1038/ismej.2012.8

Introduction to the human gut microbiota
journal, May 2017

  • Thursby, Elizabeth; Juge, Nathalie
  • Biochemical Journal, Vol. 474, Issue 11
  • DOI: 10.1042/BCJ20160510

Economic and geographic drivers of wildlife consumption in rural Africa
journal, August 2011

  • Brashares, J. S.; Golden, C. D.; Weinbaum, K. Z.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 34
  • DOI: 10.1073/pnas.1011526108

Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis---One Species on the Basis of Genetic Evidence
journal, June 2000


Wildlife Trade and Global Disease Emergence
journal, July 2005

  • Karesh, William B.; Cook, Robert A.; Bennett, Elizabeth L.
  • Emerging Infectious Diseases, Vol. 11, Issue 7
  • DOI: 10.3201/eid1107.050194

A Comparative Review on Microbiota Manipulation: Lessons From Fish, Plants, Livestock, and Human Research
journal, September 2018

  • Brugman, Sylvia; Ikeda-Ohtsubo, Wakako; Braber, Saskia
  • Frontiers in Nutrition, Vol. 5
  • DOI: 10.3389/fnut.2018.00080

Enabling the democratization of the genomics revolution with a fully integrated web-based bioinformatics platform
journal, November 2016

  • Li, Po-E; Lo, Chien-Chi; Anderson, Joseph J.
  • Nucleic Acids Research, Vol. 45, Issue 1
  • DOI: 10.1093/nar/gkw1027