Title: Multi-omic analyses of exogenous nutrient bag decomposition by the black morel Morchella importuna reveal sustained carbon acquisition and transferring

Abstract

The black morel (Morchella importuna Kuo, O'Donnell and Volk) was once an uncultivable wild mushroom, until the development of exogenous nutrient bag (ENB), making its agricultural production quite feasible and stable. To date, how the nutritional acquisition of the morel mycelium is fulfilled to trigger its fruiting remains unknown. To investigate the mechanisms involved in ENB decomposition, the genome of a cultivable morel strain (M. importuna SCYDJ1-A1) was sequenced and the genes coding for the decay apparatus were identified. Expression of the encoded carbohydrate-active enzymes (CAZymes) was then analyzed by metatranscriptomics and metaproteomics in combination with biochemical assays. The results show that a diverse set of hydrolytic and redox CAZymes secreted by the morel mycelium is the main force driving the substrate decomposition. Plant polysaccharides such as starch and cellulose present in ENB substrate (wheat grains plus rice husks) were rapidly degraded, whereas triglycerides were accumulated initially and consumed later. ENB decomposition led to a rapid increase in the organic carbon content in the surface soil of the mushroom bed, which was thereafter consumed during morel fruiting. In contrast to the high carbon consumption, no significant acquisition of nitrogen was observed. Finally, our findings contribute to an increasingly detailed portraitmore » of molecular features triggering morel fruiting.« less

Authors:

Tan, Hao  ^[1];

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Kohler, Annegret ^[2]; Miao, Renyun ^[1]; Liu, Tianhai ^[1]; Zhang, Qiang ^[1]; Zhang, Bo ^[1]; Jiang, Lin ^[1]; Wang, Yong ^[1]; Xie, Liyuan ^[1]; Tang, Jie ^[1]; Li, Xiaolin ^[1]; Liu, Lixu ^[1]; Grigoriev, Igor V. ^[3]; Daum, Chris ^[3]; LaButti, Kurt ^[3]; Lipzen, Anna ^[3]; Kuo, Alan ^[4]; Morin, Emmanuelle ^[2]; Drula, Elodie ^[5]; Henrissat, Bernard ^[5] more »; Wang, Bo ^[1]; Huang, Zhongqian ^[1]; Gan, Bingcheng ^[1]; Peng, Weihong ^[1]; Martin, Francis M. ^[2] « less

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+ Show Author Affiliations

1. Sichuan Academy of Agricultural Sciences, Chengdu (China); Ministry of Agriculture, Chengdu (China)
2. Univ. de Lorraine, Champenoux (France)
3. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Univ. of California, Berkeley, CA (United States)
4. US Department of Energy Joint Genome Institute Walnut Creek CA USA; Department of Plant and Microbial BiologyUniversity of California Berkeley Berkeley CA USA
5. Aix‐Marseille Univ. (France); Inst. National de la Recherche Agronomique, Marseille (France); King Abdulaziz Univ. Jeddah (Saudi Arabia)

Publication Date:

2019-07-25

Research Org.:

Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Sponsoring Org.:

USDOE Office of Science (SC)

OSTI Identifier:

1603530

Grant/Contract Number:  

AC02-05CH11231

Resource Type:

Accepted Manuscript

Journal Name:

Environmental Microbiology

Additional Journal Information:

Journal Volume: 21; Journal Issue: 10; Journal ID: ISSN 1462-2912

Publisher:

Wiley

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES