Occurrence of Thermophilic Microorganisms in Different Full Scale Biogas Plants

Kushkevych, Ivan; Cejnar, Jiri; Vitezova, Monika; Vitez, Tomas; Dordevic, Dani; Bomble, Yannick J

doi:10.3390/ijms21010283

Title: Occurrence of Thermophilic Microorganisms in Different Full Scale Biogas Plants

Journal Article · 31 December 2019 · International Journal of Molecular Sciences (Online)

DOI: https://doi.org/10.3390/ijms21010283 · OSTI ID:1603254

^[1]; Cejnar, Jiri ^[1]; Vitezova, Monika ^[1]; Vitez, Tomas ^[2];

^[3]; Bomble, Yannick J ^[4]

Masaryk Univ. (Czech Republic)
Mendel Univ. (Czech Republic). Dept. of Agricultural, Food and Environmental Engineering, Faculty of AgriSciences
Univ. of Veterinary and Pharmaceutical Sciences (Czech Republic)
National Renewable Energy Laboratory (NREL), Golden, CO (United States)

In recent years, various substrates have been tested to increase the sustainable production of biomethane. The effect of these substrates on methanogenesis has been investigated mainly in small volume fermenters and were, for the most part, focused on studying the diversity of mesophilic microorganisms. However, studies of thermophilic communities in large scale operating mesophilic biogas plants do not yet exist. Methods: Microbiological, biochemical, biophysical methods, and statistical analysis were used to track thermophilic communities in mesophilic anaerobic digesters. Results: The diversity of the main thermophile genera in eight biogas plants located in the Czech Republic using different input substrates was investigated. In total, 19 thermophilic genera were detected after 16S rRNA gene sequencing. The highest percentage (40.8%) of thermophiles was found in the Modrice biogas plant where the input substrate was primary sludge and biological sludge (50/50, w/w %). The smallest percentage (1.87%) of thermophiles was found in the Cejč biogas plant with the input substrate being maize silage and liquid pig manure (80/20, w/w %). Conclusions: The composition of the anaerobic consortia in anaerobic digesters is an important factor for the biogas plant operator. The present study can help characterizing the impact of input feeds on the composition of microbial communities in these plants.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1603254

Report Number(s):: NREL-JA-2700-75825; IJMCFK

Journal Information:: International Journal of Molecular Sciences (Online), Vol. 21, Issue 1; ISSN 1422-0067

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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Title: Occurrence of Thermophilic Microorganisms in Different Full Scale Biogas Plants

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