Occurrence of Thermophilic Microorganisms in Different Full Scale Biogas Plants
Abstract
In recent years, various substrates have been tested to increase the sustainable production of biomethane. The effect of these substrates on methanogenesis has been investigated mainly in small volume fermenters and were, for the most part, focused on studying the diversity of mesophilic microorganisms. However, studies of thermophilic communities in large scale operating mesophilic biogas plants do not yet exist. Methods: Microbiological, biochemical, biophysical methods, and statistical analysis were used to track thermophilic communities in mesophilic anaerobic digesters. Results: The diversity of the main thermophile genera in eight biogas plants located in the Czech Republic using different input substrates was investigated. In total, 19 thermophilic genera were detected after 16S rRNA gene sequencing. The highest percentage (40.8%) of thermophiles was found in the Modrice biogas plant where the input substrate was primary sludge and biological sludge (50/50, w/w %). The smallest percentage (1.87%) of thermophiles was found in the Cejč biogas plant with the input substrate being maize silage and liquid pig manure (80/20, w/w %). Conclusions: The composition of the anaerobic consortia in anaerobic digesters is an important factor for the biogas plant operator. The present study can help characterizing the impact of input feeds on the composition ofmore »
- Authors:
-
[1];
[3];
- Masaryk Univ. (Czech Republic)
- Mendel Univ. (Czech Republic). Dept. of Agricultural, Food and Environmental Engineering, Faculty of AgriSciences
- Univ. of Veterinary and Pharmaceutical Sciences (Czech Republic)
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Publication Date:
- Research Org.:
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
- OSTI Identifier:
- 1603254
- Report Number(s):
- NREL-JA-2700-75825
Journal ID: ISSN 1422-0067; IJMCFK
- Grant/Contract Number:
- AC36-08GO28308
- Resource Type:
- Accepted Manuscript
- Journal Name:
- International Journal of Molecular Sciences (Online)
- Additional Journal Information:
- Journal Name: International Journal of Molecular Sciences (Online); Journal Volume: 21; Journal Issue: 1; Journal ID: ISSN 1422-0067
- Publisher:
- MDPI
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; renewable energy; biogas; Illumina sequencing; thermophilic microorganisms; anaerobic digesters
Citation Formats
Kushkevych, Ivan, Cejnar, Jiri, Vitezova, Monika, Vitez, Tomas, Dordevic, Dani, and Bomble, Yannick J. Occurrence of Thermophilic Microorganisms in Different Full Scale Biogas Plants. United States: N. p., 2019.
Web. doi:10.3390/ijms21010283.
Kushkevych, Ivan, Cejnar, Jiri, Vitezova, Monika, Vitez, Tomas, Dordevic, Dani, & Bomble, Yannick J. Occurrence of Thermophilic Microorganisms in Different Full Scale Biogas Plants. United States. doi:https://doi.org/10.3390/ijms21010283
Kushkevych, Ivan, Cejnar, Jiri, Vitezova, Monika, Vitez, Tomas, Dordevic, Dani, and Bomble, Yannick J. Tue .
"Occurrence of Thermophilic Microorganisms in Different Full Scale Biogas Plants". United States. doi:https://doi.org/10.3390/ijms21010283. https://www.osti.gov/servlets/purl/1603254.
@article{osti_1603254,
title = {Occurrence of Thermophilic Microorganisms in Different Full Scale Biogas Plants},
author = {Kushkevych, Ivan and Cejnar, Jiri and Vitezova, Monika and Vitez, Tomas and Dordevic, Dani and Bomble, Yannick J},
abstractNote = {In recent years, various substrates have been tested to increase the sustainable production of biomethane. The effect of these substrates on methanogenesis has been investigated mainly in small volume fermenters and were, for the most part, focused on studying the diversity of mesophilic microorganisms. However, studies of thermophilic communities in large scale operating mesophilic biogas plants do not yet exist. Methods: Microbiological, biochemical, biophysical methods, and statistical analysis were used to track thermophilic communities in mesophilic anaerobic digesters. Results: The diversity of the main thermophile genera in eight biogas plants located in the Czech Republic using different input substrates was investigated. In total, 19 thermophilic genera were detected after 16S rRNA gene sequencing. The highest percentage (40.8%) of thermophiles was found in the Modrice biogas plant where the input substrate was primary sludge and biological sludge (50/50, w/w %). The smallest percentage (1.87%) of thermophiles was found in the Cejč biogas plant with the input substrate being maize silage and liquid pig manure (80/20, w/w %). Conclusions: The composition of the anaerobic consortia in anaerobic digesters is an important factor for the biogas plant operator. The present study can help characterizing the impact of input feeds on the composition of microbial communities in these plants.},
doi = {10.3390/ijms21010283},
journal = {International Journal of Molecular Sciences (Online)},
number = 1,
volume = 21,
place = {United States},
year = {2019},
month = {12}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Development of a methanogenic process to degrade exhaustively the organic fraction of municipal “grey waste” under thermophilic and hyperthermophilic conditions
journal, February 2000
- Scherer, P. A.; Vollmer, G. -R.; Fakhouri, T.
- Water Science and Technology, Vol. 41, Issue 3
Non-aceticlastic methanogenesis from acetate: acetate oxidation by a thermophilic syntrophic coculture
journal, July 1984
- Zinder, Stephen H.; Koch, Markus
- Archives of Microbiology, Vol. 138, Issue 3
Oceanotoga teriensis gen. nov., sp. nov., a thermophilic bacterium isolated from offshore oil-producing wells
journal, April 2010
- Jayasinghearachchi, H. S.; Lal, B.
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 61, Issue 3
Toxicity of hydrogen sulfide toward sulfate-reducing bacteria Desulfovibrio piger Vib-7
journal, February 2019
- Kushkevych, Ivan; Dordević, Dani; Vítězová, Monika
- Archives of Microbiology, Vol. 201, Issue 3
Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data
journal, April 2012
- Kearse, M.; Moir, R.; Wilson, A.
- Bioinformatics, Vol. 28, Issue 12
Cross-correlation analysis of the Desulfovibrio growth parameters of intestinal species isolated from people with colitis
journal, September 2018
- Kushkevych, Ivan; Dordević, Dani; Vítězová, Monika
- Biologia, Vol. 73, Issue 11
Clustal W and Clustal X version 2.0
journal, September 2007
- Larkin, M. A.; Blackshields, G.; Brown, N. P.
- Bioinformatics, Vol. 23, Issue 21, p. 2947-2948
A new combination of substrates: biogas production and diversity of the methanogenic microorganisms
journal, April 2018
- Kushkevych, Ivan; Vítězová, Monika; Vítěz, Tomáš
- Open Life Sciences, Vol. 13, Issue 1
Analysis of physiological parameters of Desulfovibrio strains from individuals with colitis
journal, January 2019
- Kushkevych, Ivan; Dordević, Dani; Kollár, Peter
- Open Life Sciences, Vol. 13, Issue 1
The roles of acetotrophic and hydrogenotrophic methanogens during anaerobic conversion of biomass to methane: a review
journal, January 2008
- Demirel, Burak; Scherer, Paul
- Reviews in Environmental Science and Bio/Technology, Vol. 7, Issue 2
Effect of temperature increase from 55 to 65°C on performance and microbial population dynamics of an anaerobic reactor treating cattle manure
journal, July 2001
- Ahring, Birgitte K.; Ibrahim, Ashraf A.; Mladenovska, Zuzana
- Water Research, Vol. 35, Issue 10
Contribution of hydrogen to methane production and control of hydrogen concentrations in methanogenic soils and sediments
journal, March 1999
- Conrad, R.
- FEMS Microbiology Ecology, Vol. 28, Issue 3
The diversity of sulfate-reducing bacteria in the seven bioreactors
journal, April 2018
- Kushkevych, Ivan; Kováč, Jozef; Vítězová, Monika
- Archives of Microbiology, Vol. 200, Issue 6
Activity of Na+/K+-activated Mg2+-dependent ATP-hydrolase in the cell-free extracts of the sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9
journal, January 2015
- Kushkevych, Ivan; Fafula, Roman; Parák, Tomáš
- Acta Veterinaria Brno, Vol. 84, Issue 1
Thermophilic methane production and oxidation in compost
journal, April 2005
- Jäckel, Udo; Thummes, Kathrin; Kämpfer, Peter
- FEMS Microbiology Ecology, Vol. 52, Issue 2
Methanogenic population dynamics during start-up of anaerobic digesters treating municipal solid waste and biosolids
journal, February 1998
- Griffin, Matt E.; McMahon, Katherine D.; Mackie, Roderick I.
- Biotechnology and Bioengineering, Vol. 57, Issue 3
Activity and kinetic properties of phosphotransacetylase from intestinal sulfate-reducing bacteria
journal, January 2015
- Kushkevych, Ivan V.
- Acta Biochimica Polonica, Vol. 62, Issue 1
QIIME allows analysis of high-throughput community sequencing data
journal, April 2010
- Caporaso, J. Gregory; Kuczynski, Justin; Stombaugh, Jesse
- Nature Methods, Vol. 7, Issue 5
Basic local alignment search tool
journal, October 1990
- Altschul, Stephen F.; Gish, Warren; Miller, Webb
- Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410
Biogas production: current state and perspectives
journal, September 2009
- Weiland, Peter
- Applied Microbiology and Biotechnology, Vol. 85, Issue 4
Mesophilic syntrophic acetate oxidation during methane formation by a triculture at high ammonium concentration
journal, July 1994
- Schnürer, Anna; Houwen, Frans P.; Svensson, Bo H.
- Archives of Microbiology, Vol. 162, Issue 1-2
Acetogenic microorganisms in operating biogas plants depending on substrate combinations
journal, May 2019
- Kushkevych, Ivan; Kobzová, Eliška; Vítězová, Monika
- Biologia, Vol. 74, Issue 9
Phylogenetic diversity and activity of anaerobic microorganisms of high-temperature horizons of the Dagang oil field (P. R. China)
journal, January 2006
- Nazina, T. N.; Shestakova, N. M.; Grigor’yan, A. A.
- Microbiology, Vol. 75, Issue 1
Antimicrobial effect of salicylamide derivatives against intestinal sulfate-reducing bacteria
journal, April 2016
- Kushkevych, Ivan; Kollar, Peter; Ferreira, Ana Luisa
- Journal of Applied Biomedicine, Vol. 14, Issue 2
History of discovery of the first hyperthermophiles
journal, August 2006
- Stetter, Karl O.
- Extremophiles, Vol. 10, Issue 5
On the biochemistry of the methane fermentation
journal, January 1936
- Barker, H. Albert
- Archiv f�r Mikrobiologie, Vol. 7, Issue 1-5
Effect of selected 8-hydroxyquinoline-2-carboxanilides on viability and sulfate metabolism of Desulfovibrio piger
journal, August 2018
- Kushkevych, Ivan; Vítězová, Monika; Kos, Jiří
- Journal of Applied Biomedicine, Vol. 16, Issue 3
Design of 16S rRNA gene primers for 454 pyrosequencing of the human foregut microbiome
journal, January 2010
- Nossa, Carlos W.
- World Journal of Gastroenterology, Vol. 16, Issue 33
Physiology, Ecology, Phylogeny, and Genomics of Microorganisms Capable of Syntrophic Metabolism
journal, March 2008
- McInerney, Michael J.; Struchtemeyer, Christopher G.; Sieber, Jessica
- Annals of the New York Academy of Sciences, Vol. 1125, Issue 1
Study of methanogenesis during bioutilization of plant residuals
journal, February 2005
- Ilyin, V. K.; Korniushenkova, I. N.; Starkova, L. V.
- Acta Astronautica, Vol. 56, Issue 4
Bacteriological composition and structure of granular sludge adapted to different substrates.
journal, January 1991
- Grotenhuis, J. T.; Smit, M.; Plugge, C. M.
- Applied and Environmental Microbiology, Vol. 57, Issue 7
Thermogymnomonas acidicola gen. nov., sp. nov., a novel thermoacidophilic, cell wall-less archaeon in the order Thermoplasmatales, isolated from a solfataric soil in Hakone, Japan
journal, November 2007
- Itoh, T.; Yoshikawa, N.; Takashina, T.
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 57, Issue 11
Kinetic properties of growth of intestinal sulphate-reducing bacteria isolated from healthy mice and mice with ulcerative colitis
journal, January 2017
- Kushkevych, Ivan; Vítězová, Monika; Fedrová, Pavla
- Acta Veterinaria Brno, Vol. 86, Issue 4
Analysis of pH dose-dependent growth of sulfate-reducing bacteria
journal, January 2019
- Kushkevych, Ivan; Dordević, Dani; Vítězová, Monika
- Open Medicine, Vol. 14, Issue 1
Production of biogas: relationship between methanogenic and sulfate-reducing microorganisms
journal, April 2017
- Kushkevych, Ivan; Vítězová, Monika; Vítěz, Tomáš
- Open Life Sciences, Vol. 12, Issue 1
Kinetic properties of pyruvate ferredoxin oxidoreductase of intestinal sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9
journal, January 2015
- Kushkevych, Ivan V.
- Polish Journal of Microbiology, Vol. 64, Issue 2