Occurrence of Thermophilic Microorganisms in Different Full Scale Biogas Plants
Abstract
In recent years, various substrates have been tested to increase the sustainable production of biomethane. The effect of these substrates on methanogenesis has been investigated mainly in small volume fermenters and were, for the most part, focused on studying the diversity of mesophilic microorganisms. However, studies of thermophilic communities in large scale operating mesophilic biogas plants do not yet exist. Methods: Microbiological, biochemical, biophysical methods, and statistical analysis were used to track thermophilic communities in mesophilic anaerobic digesters. Results: The diversity of the main thermophile genera in eight biogas plants located in the Czech Republic using different input substrates was investigated. In total, 19 thermophilic genera were detected after 16S rRNA gene sequencing. The highest percentage (40.8%) of thermophiles was found in the Modrice biogas plant where the input substrate was primary sludge and biological sludge (50/50, w/w %). The smallest percentage (1.87%) of thermophiles was found in the Cejč biogas plant with the input substrate being maize silage and liquid pig manure (80/20, w/w %). Conclusions: The composition of the anaerobic consortia in anaerobic digesters is an important factor for the biogas plant operator. The present study can help characterizing the impact of input feeds on the composition ofmore »
- Authors:
-
[1];
[3];
- Masaryk Univ. (Czech Republic)
- Mendel Univ. (Czech Republic). Dept. of Agricultural, Food and Environmental Engineering, Faculty of AgriSciences
- Univ. of Veterinary and Pharmaceutical Sciences (Czech Republic)
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Publication Date:
- Research Org.:
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1603254
- Report Number(s):
- NREL-JA-2700-75825
Journal ID: ISSN 1422-0067; IJMCFK
- Grant/Contract Number:
- AC36-08GO28308
- Resource Type:
- Accepted Manuscript
- Journal Name:
- International Journal of Molecular Sciences (Online)
- Additional Journal Information:
- Journal Name: International Journal of Molecular Sciences (Online); Journal Volume: 21; Journal Issue: 1; Journal ID: ISSN 1422-0067
- Publisher:
- MDPI
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; renewable energy; biogas; Illumina sequencing; thermophilic microorganisms; anaerobic digesters
Citation Formats
Kushkevych, Ivan, Cejnar, Jiri, Vitezova, Monika, Vitez, Tomas, Dordevic, Dani, and Bomble, Yannick J. Occurrence of Thermophilic Microorganisms in Different Full Scale Biogas Plants. United States: N. p., 2019.
Web. doi:10.3390/ijms21010283.
Kushkevych, Ivan, Cejnar, Jiri, Vitezova, Monika, Vitez, Tomas, Dordevic, Dani, & Bomble, Yannick J. Occurrence of Thermophilic Microorganisms in Different Full Scale Biogas Plants. United States. https://doi.org/10.3390/ijms21010283
Kushkevych, Ivan, Cejnar, Jiri, Vitezova, Monika, Vitez, Tomas, Dordevic, Dani, and Bomble, Yannick J. Tue .
"Occurrence of Thermophilic Microorganisms in Different Full Scale Biogas Plants". United States. https://doi.org/10.3390/ijms21010283. https://www.osti.gov/servlets/purl/1603254.
@article{osti_1603254,
title = {Occurrence of Thermophilic Microorganisms in Different Full Scale Biogas Plants},
author = {Kushkevych, Ivan and Cejnar, Jiri and Vitezova, Monika and Vitez, Tomas and Dordevic, Dani and Bomble, Yannick J},
abstractNote = {In recent years, various substrates have been tested to increase the sustainable production of biomethane. The effect of these substrates on methanogenesis has been investigated mainly in small volume fermenters and were, for the most part, focused on studying the diversity of mesophilic microorganisms. However, studies of thermophilic communities in large scale operating mesophilic biogas plants do not yet exist. Methods: Microbiological, biochemical, biophysical methods, and statistical analysis were used to track thermophilic communities in mesophilic anaerobic digesters. Results: The diversity of the main thermophile genera in eight biogas plants located in the Czech Republic using different input substrates was investigated. In total, 19 thermophilic genera were detected after 16S rRNA gene sequencing. The highest percentage (40.8%) of thermophiles was found in the Modrice biogas plant where the input substrate was primary sludge and biological sludge (50/50, w/w %). The smallest percentage (1.87%) of thermophiles was found in the Cejč biogas plant with the input substrate being maize silage and liquid pig manure (80/20, w/w %). Conclusions: The composition of the anaerobic consortia in anaerobic digesters is an important factor for the biogas plant operator. The present study can help characterizing the impact of input feeds on the composition of microbial communities in these plants.},
doi = {10.3390/ijms21010283},
journal = {International Journal of Molecular Sciences (Online)},
number = 1,
volume = 21,
place = {United States},
year = {2019},
month = {12}
}
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