Effect of iron and silica nanoparticles’ size on in vitro human skin binding and penetration
Abstract
This in vitro skin study determined absorption, diffusion, and binding rates of four [14C]-labeled nanoparticles (NPs): 12 nm Fe3O4, 32 nm Fe3O4@SiO2, 33 nm SiO2, and 78 nm SiO2in each layer of human cadaver skin. In vitro microdialysis device and flow-through skin diffusion system were used to measure the binding affinity to the stratum corneum (SC) and permeability into/through skin layer of the four NPs with different physical–chemical properties, respectively, in short (30 min) and/or long (24 hours) exposures. Results show that NP size is an important factor affecting NP percutaneous absorption. The 12 nm Fe3O4 NPs reached the SC and viable epidermis; 32 nm Fe3O4@SiO2core/shell NPs only reached SC. However, 33 nm and 78 nm silica NPs did not permeate SC. Similar patterns were observed for NP binding affinity to SC and dermatopharmacokinetic analysis using the tape stripping method. The binding affinity determination may be a useful method to efficiently screen skin penetration of NPs.
- Authors:
-
- Univ. of California San Francisco, CA (United States)
- Aramco Services Company, Cambridge, MA (United States)
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Publication Date:
- Research Org.:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE National Nuclear Security Administration (NNSA)
- OSTI Identifier:
- 1603236
- Report Number(s):
- LLNL-JRNL-757207
Journal ID: ISSN 2397-8473; 944926
- Grant/Contract Number:
- AC52-07NA27344; HDTRA1-14-0005
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Toxicology Research and Application
- Additional Journal Information:
- Journal Volume: 3; Journal Issue: na; Journal ID: ISSN 2397-8473
- Publisher:
- Sage
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Iron oxide and silica oxide nanoparticles; in vitro human skin absorption; stratum corneum binding; dermatopharmacokinetic; tape stripping; nanoparticle size-related penetration
Citation Formats
Jung, Eui, Hui, Xiaoying, Zhu, Hanjiang, Zhang, Alissa, Wang, Wei, Buchholz, Bruce, and Maibach, Howard. Effect of iron and silica nanoparticles’ size on in vitro human skin binding and penetration. United States: N. p., 2019.
Web. doi:10.1177/2397847319893054.
Jung, Eui, Hui, Xiaoying, Zhu, Hanjiang, Zhang, Alissa, Wang, Wei, Buchholz, Bruce, & Maibach, Howard. Effect of iron and silica nanoparticles’ size on in vitro human skin binding and penetration. United States. https://doi.org/10.1177/2397847319893054
Jung, Eui, Hui, Xiaoying, Zhu, Hanjiang, Zhang, Alissa, Wang, Wei, Buchholz, Bruce, and Maibach, Howard. Fri .
"Effect of iron and silica nanoparticles’ size on in vitro human skin binding and penetration". United States. https://doi.org/10.1177/2397847319893054. https://www.osti.gov/servlets/purl/1603236.
@article{osti_1603236,
title = {Effect of iron and silica nanoparticles’ size on in vitro human skin binding and penetration},
author = {Jung, Eui and Hui, Xiaoying and Zhu, Hanjiang and Zhang, Alissa and Wang, Wei and Buchholz, Bruce and Maibach, Howard},
abstractNote = {This in vitro skin study determined absorption, diffusion, and binding rates of four [14C]-labeled nanoparticles (NPs): 12 nm Fe3O4, 32 nm Fe3O4@SiO2, 33 nm SiO2, and 78 nm SiO2in each layer of human cadaver skin. In vitro microdialysis device and flow-through skin diffusion system were used to measure the binding affinity to the stratum corneum (SC) and permeability into/through skin layer of the four NPs with different physical–chemical properties, respectively, in short (30 min) and/or long (24 hours) exposures. Results show that NP size is an important factor affecting NP percutaneous absorption. The 12 nm Fe3O4 NPs reached the SC and viable epidermis; 32 nm Fe3O4@SiO2core/shell NPs only reached SC. However, 33 nm and 78 nm silica NPs did not permeate SC. Similar patterns were observed for NP binding affinity to SC and dermatopharmacokinetic analysis using the tape stripping method. The binding affinity determination may be a useful method to efficiently screen skin penetration of NPs.},
doi = {10.1177/2397847319893054},
journal = {Toxicology Research and Application},
number = na,
volume = 3,
place = {United States},
year = {Fri Dec 27 00:00:00 EST 2019},
month = {Fri Dec 27 00:00:00 EST 2019}
}
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