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Title: Effect of iron and silica nanoparticles’ size on in vitro human skin binding and penetration

Abstract

This in vitro skin study determined absorption, diffusion, and binding rates of four [14C]-labeled nanoparticles (NPs): 12 nm Fe3O4, 32 nm Fe3O4@SiO2, 33 nm SiO2, and 78 nm SiO2in each layer of human cadaver skin. In vitro microdialysis device and flow-through skin diffusion system were used to measure the binding affinity to the stratum corneum (SC) and permeability into/through skin layer of the four NPs with different physical–chemical properties, respectively, in short (30 min) and/or long (24 hours) exposures. Results show that NP size is an important factor affecting NP percutaneous absorption. The 12 nm Fe3O4 NPs reached the SC and viable epidermis; 32 nm Fe3O4@SiO2core/shell NPs only reached SC. However, 33 nm and 78 nm silica NPs did not permeate SC. Similar patterns were observed for NP binding affinity to SC and dermatopharmacokinetic analysis using the tape stripping method. The binding affinity determination may be a useful method to efficiently screen skin penetration of NPs.

Authors:
 [1]; ORCiD logo [1];  [1];  [1];  [2];  [3];  [1]
  1. Univ. of California San Francisco, CA (United States)
  2. Aramco Services Company, Cambridge, MA (United States)
  3. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE National Nuclear Security Administration (NNSA)
OSTI Identifier:
1603236
Report Number(s):
LLNL-JRNL-757207
Journal ID: ISSN 2397-8473; 944926
Grant/Contract Number:  
AC52-07NA27344; HDTRA1-14-0005
Resource Type:
Accepted Manuscript
Journal Name:
Toxicology Research and Application
Additional Journal Information:
Journal Volume: 3; Journal Issue: na; Journal ID: ISSN 2397-8473
Publisher:
Sage
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Iron oxide and silica oxide nanoparticles; in vitro human skin absorption; stratum corneum binding; dermatopharmacokinetic; tape stripping; nanoparticle size-related penetration

Citation Formats

Jung, Eui, Hui, Xiaoying, Zhu, Hanjiang, Zhang, Alissa, Wang, Wei, Buchholz, Bruce, and Maibach, Howard. Effect of iron and silica nanoparticles’ size on in vitro human skin binding and penetration. United States: N. p., 2019. Web. doi:10.1177/2397847319893054.
Jung, Eui, Hui, Xiaoying, Zhu, Hanjiang, Zhang, Alissa, Wang, Wei, Buchholz, Bruce, & Maibach, Howard. Effect of iron and silica nanoparticles’ size on in vitro human skin binding and penetration. United States. doi:https://doi.org/10.1177/2397847319893054
Jung, Eui, Hui, Xiaoying, Zhu, Hanjiang, Zhang, Alissa, Wang, Wei, Buchholz, Bruce, and Maibach, Howard. Fri . "Effect of iron and silica nanoparticles’ size on in vitro human skin binding and penetration". United States. doi:https://doi.org/10.1177/2397847319893054. https://www.osti.gov/servlets/purl/1603236.
@article{osti_1603236,
title = {Effect of iron and silica nanoparticles’ size on in vitro human skin binding and penetration},
author = {Jung, Eui and Hui, Xiaoying and Zhu, Hanjiang and Zhang, Alissa and Wang, Wei and Buchholz, Bruce and Maibach, Howard},
abstractNote = {This in vitro skin study determined absorption, diffusion, and binding rates of four [14C]-labeled nanoparticles (NPs): 12 nm Fe3O4, 32 nm Fe3O4@SiO2, 33 nm SiO2, and 78 nm SiO2in each layer of human cadaver skin. In vitro microdialysis device and flow-through skin diffusion system were used to measure the binding affinity to the stratum corneum (SC) and permeability into/through skin layer of the four NPs with different physical–chemical properties, respectively, in short (30 min) and/or long (24 hours) exposures. Results show that NP size is an important factor affecting NP percutaneous absorption. The 12 nm Fe3O4 NPs reached the SC and viable epidermis; 32 nm Fe3O4@SiO2core/shell NPs only reached SC. However, 33 nm and 78 nm silica NPs did not permeate SC. Similar patterns were observed for NP binding affinity to SC and dermatopharmacokinetic analysis using the tape stripping method. The binding affinity determination may be a useful method to efficiently screen skin penetration of NPs.},
doi = {10.1177/2397847319893054},
journal = {Toxicology Research and Application},
number = na,
volume = 3,
place = {United States},
year = {2019},
month = {12}
}

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