Effect of iron and silica nanoparticles’ size on in vitro human skin binding and penetration

Jung, Eui; Hui, Xiaoying; Zhu, Hanjiang; Zhang, Alissa; Wang, Wei; Buchholz, Bruce; Maibach, Howard

doi:10.1177/2397847319893054

Title: Effect of iron and silica nanoparticles’ size on in vitro human skin binding and penetration

Journal Article · 26 December 2019 · Toxicology Research and Application

DOI: https://doi.org/10.1177/2397847319893054 · OSTI ID:1603236

Jung, Eui ^[1];

^[1]; Zhu, Hanjiang ^[1]; Zhang, Alissa ^[1]; Wang, Wei ^[2]; Buchholz, Bruce ^[3]; Maibach, Howard ^[1]

Univ. of California San Francisco, CA (United States)
Aramco Services Company, Cambridge, MA (United States)
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

This in vitro skin study determined absorption, diffusion, and binding rates of four [14C]-labeled nanoparticles (NPs): 12 nm Fe₃O₄, 32 nm Fe₃O₄@SiO₂, 33 nm SiO₂, and 78 nm SiO₂in each layer of human cadaver skin. In vitro microdialysis device and flow-through skin diffusion system were used to measure the binding affinity to the stratum corneum (SC) and permeability into/through skin layer of the four NPs with different physical–chemical properties, respectively, in short (30 min) and/or long (24 hours) exposures. Results show that NP size is an important factor affecting NP percutaneous absorption. The 12 nm Fe₃O₄ NPs reached the SC and viable epidermis; 32 nm Fe₃O₄@SiO₂core/shell NPs only reached SC. However, 33 nm and 78 nm silica NPs did not permeate SC. Similar patterns were observed for NP binding affinity to SC and dermatopharmacokinetic analysis using the tape stripping method. The binding affinity determination may be a useful method to efficiently screen skin penetration of NPs.

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Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE National Nuclear Security Administration (NNSA)

Grant/Contract Number:: AC52-07NA27344

OSTI ID:: 1603236

Report Number(s):: LLNL-JRNL-757207; 944926

Journal Information:: Toxicology Research and Application, Journal Name: Toxicology Research and Application Journal Issue: na Vol. 3; ISSN 2397-8473

Publisher:: SageCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Iron oxide and silica oxide nanoparticles
dermatopharmacokinetic
in vitro human skin absorption
nanoparticle size-related penetration
stratum corneum binding
tape stripping

Title: Effect of iron and silica nanoparticles’ size on in vitro human skin binding and penetration

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References (34)

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