The small secreted effector protein MiSSP7.6 of Laccaria bicolor is required for the establishment of ectomycorrhizal symbiosis

Kang, Heng; Chen, Xin; Kemppainen, Minna; Pardo, Alejandro G.; Veneault‐Fourrey, Claire; Kohler, Annegret; Martin, Francis M.

doi:10.1111/1462-2920.14959

Title: The small secreted effector protein MiSSP7.6 of Laccaria bicolor is required for the establishment of ectomycorrhizal symbiosis

Journal Article · 01 March 2020 · Environmental Microbiology

DOI: https://doi.org/10.1111/1462-2920.14959 · OSTI ID:1602327

^[1]; Chen, Xin ^[2]; Kemppainen, Minna ^[3]; Pardo, Alejandro G. ^[3];

^[4]; Kohler, Annegret ^[4]; Martin, Francis M. ^[4]

College of Plant Science and Technology, Huazhong Agricultural University Wuhan 430070 China, University of Lorraine, Institut National de la Recherche Agronomique, UMR Interactions Arbres/Microorganismes, Laboratoire d'Excellence ARBRE, Centre INRA‐Grand Est Champenoux France
College of Plant Science and Technology, Huazhong Agricultural University Wuhan 430070 China
Laboratorio de Micología Molecular, Instituto de Microbiología Básica y Aplicada, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) Bernal Provincia de Buenos Aires Argentina
University of Lorraine, Institut National de la Recherche Agronomique, UMR Interactions Arbres/Microorganismes, Laboratoire d'Excellence ARBRE, Centre INRA‐Grand Est Champenoux France

Summary To establish and maintain a symbiotic relationship, the ectomycorrhizal fungus Laccaria bicolor releases mycorrhiza‐induced small secreted proteins (MiSSPs) into host roots. Here, we have functionally characterized the M YCORRHIZA‐ i NDUCED S MALL S ECRETED P ROTEIN OF 7 . 6 kDa ( MiSSP7 . 6 ) from L . bicolor by assessing its induced expression in ectomycorrhizae, silencing its expression by RNAi, and tracking in planta subcellular localization of its protein product. We also carried out yeast two‐hybrid assays and bimolecular fluorescence complementation analysis to identify possible protein targets of the MiSSP7.6 effector in Populus roots. We showed that MiSSP7 . 6 expression is upregulated in ectomycorrhizal rootlets and associated extramatrical mycelium during the late stage of symbiosis development. RNAi mutants with a decreased MiSSP7 . 6 expression have a lower mycorrhization rate , suggesting a key role in the establishment of the symbiosis with plants. MiSSP7.6 is secreted, and it localizes both to the nuclei and cytoplasm in plant cells. MiSSP7.6 protein was shown to interact with two Populus Trihelix transcription factors. Furthermore, when coexpressed with one of the Trihelix transcription factors, MiSSP7.6 is localized to plant nuclei only. Our data suggest that MiSSP7.6 is a novel secreted symbiotic effector and is a potential determinant for ectomycorrhiza formation.

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Sponsoring Organization:: USDOE

OSTI ID:: 1602327

Journal Information:: Environmental Microbiology, Journal Name: Environmental Microbiology Journal Issue: 4 Vol. 22; ISSN 1462-2912

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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