The small secreted effector protein MiSSP7.6 of Laccaria bicolor is required for the establishment of ectomycorrhizal symbiosis
Abstract
Summary To establish and maintain a symbiotic relationship, the ectomycorrhizal fungus Laccaria bicolor releases mycorrhiza‐induced small secreted proteins (MiSSPs) into host roots. Here, we have functionally characterized the M YCORRHIZA‐ i NDUCED S MALL S ECRETED P ROTEIN OF 7 . 6 kDa ( MiSSP7 . 6 ) from L . bicolor by assessing its induced expression in ectomycorrhizae, silencing its expression by RNAi, and tracking in planta subcellular localization of its protein product. We also carried out yeast two‐hybrid assays and bimolecular fluorescence complementation analysis to identify possible protein targets of the MiSSP7.6 effector in Populus roots. We showed that MiSSP7 . 6 expression is upregulated in ectomycorrhizal rootlets and associated extramatrical mycelium during the late stage of symbiosis development. RNAi mutants with a decreased MiSSP7 . 6 expression have a lower mycorrhization rate , suggesting a key role in the establishment of the symbiosis with plants. MiSSP7.6 is secreted, and it localizes both to the nuclei and cytoplasm in plant cells. MiSSP7.6 protein was shown to interact with two Populus Trihelix transcription factors. Furthermore, when coexpressed with one of the Trihelix transcription factors, MiSSP7.6 is localized to plant nuclei only. Our data suggest that MiSSP7.6 is a novel secretedmore »
- Authors:
-
[1];
[4];
- College of Plant Science and Technology, Huazhong Agricultural University Wuhan 430070 China, University of Lorraine, Institut National de la Recherche Agronomique, UMR Interactions Arbres/Microorganismes, Laboratoire d'Excellence ARBRE, Centre INRA‐Grand Est Champenoux France
- College of Plant Science and Technology, Huazhong Agricultural University Wuhan 430070 China
- Laboratorio de Micología Molecular, Instituto de Microbiología Básica y Aplicada, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) Bernal Provincia de Buenos Aires Argentina
- University of Lorraine, Institut National de la Recherche Agronomique, UMR Interactions Arbres/Microorganismes, Laboratoire d'Excellence ARBRE, Centre INRA‐Grand Est Champenoux France
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1602327
- Resource Type:
- Publisher's Accepted Manuscript
- Journal Name:
- Environmental Microbiology
- Additional Journal Information:
- Journal Name: Environmental Microbiology Journal Volume: 22 Journal Issue: 4; Journal ID: ISSN 1462-2912
- Publisher:
- Wiley-Blackwell
- Country of Publication:
- United Kingdom
- Language:
- English
Citation Formats
Kang, Heng, Chen, Xin, Kemppainen, Minna, Pardo, Alejandro G., Veneault‐Fourrey, Claire, Kohler, Annegret, and Martin, Francis M. The small secreted effector protein MiSSP7.6 of Laccaria bicolor is required for the establishment of ectomycorrhizal symbiosis. United Kingdom: N. p., 2020.
Web. doi:10.1111/1462-2920.14959.
Kang, Heng, Chen, Xin, Kemppainen, Minna, Pardo, Alejandro G., Veneault‐Fourrey, Claire, Kohler, Annegret, & Martin, Francis M. The small secreted effector protein MiSSP7.6 of Laccaria bicolor is required for the establishment of ectomycorrhizal symbiosis. United Kingdom. https://doi.org/10.1111/1462-2920.14959
Kang, Heng, Chen, Xin, Kemppainen, Minna, Pardo, Alejandro G., Veneault‐Fourrey, Claire, Kohler, Annegret, and Martin, Francis M. Mon .
"The small secreted effector protein MiSSP7.6 of Laccaria bicolor is required for the establishment of ectomycorrhizal symbiosis". United Kingdom. https://doi.org/10.1111/1462-2920.14959.
@article{osti_1602327,
title = {The small secreted effector protein MiSSP7.6 of Laccaria bicolor is required for the establishment of ectomycorrhizal symbiosis},
author = {Kang, Heng and Chen, Xin and Kemppainen, Minna and Pardo, Alejandro G. and Veneault‐Fourrey, Claire and Kohler, Annegret and Martin, Francis M.},
abstractNote = {Summary To establish and maintain a symbiotic relationship, the ectomycorrhizal fungus Laccaria bicolor releases mycorrhiza‐induced small secreted proteins (MiSSPs) into host roots. Here, we have functionally characterized the M YCORRHIZA‐ i NDUCED S MALL S ECRETED P ROTEIN OF 7 . 6 kDa ( MiSSP7 . 6 ) from L . bicolor by assessing its induced expression in ectomycorrhizae, silencing its expression by RNAi, and tracking in planta subcellular localization of its protein product. We also carried out yeast two‐hybrid assays and bimolecular fluorescence complementation analysis to identify possible protein targets of the MiSSP7.6 effector in Populus roots. We showed that MiSSP7 . 6 expression is upregulated in ectomycorrhizal rootlets and associated extramatrical mycelium during the late stage of symbiosis development. RNAi mutants with a decreased MiSSP7 . 6 expression have a lower mycorrhization rate , suggesting a key role in the establishment of the symbiosis with plants. MiSSP7.6 is secreted, and it localizes both to the nuclei and cytoplasm in plant cells. MiSSP7.6 protein was shown to interact with two Populus Trihelix transcription factors. Furthermore, when coexpressed with one of the Trihelix transcription factors, MiSSP7.6 is localized to plant nuclei only. Our data suggest that MiSSP7.6 is a novel secreted symbiotic effector and is a potential determinant for ectomycorrhiza formation.},
doi = {10.1111/1462-2920.14959},
journal = {Environmental Microbiology},
number = 4,
volume = 22,
place = {United Kingdom},
year = {Mon Mar 02 00:00:00 EST 2020},
month = {Mon Mar 02 00:00:00 EST 2020}
}
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https://doi.org/10.1111/1462-2920.14959
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