Quantification of Carbon Nanotube Doses in Adherent Cell Culture Assays Using UV-VIS-NIR Spectroscopy

Septiadi, Dedy; Rodriguez-Lorenzo, Laura; Balog, Sandor; Spuch-Calvar, Miguel; Spiaggia, Giovanni; Taladriz-Blanco, Patricia; Barosova, Hana; Chortarea, Savvina; Clift, Martin J.  D.; Teeguarden, Justin; Sharma, Monita; Petri-Fink, Alke; Rothen-Rutishauser, Barbara

doi:10.3390/nano9121765

Title: Quantification of Carbon Nanotube Doses in Adherent Cell Culture Assays Using UV-VIS-NIR Spectroscopy

Journal Article · 10 December 2019 · Nanomaterials

DOI: https://doi.org/10.3390/nano9121765 · OSTI ID:1601354

Septiadi, Dedy ^[1]; Rodriguez-Lorenzo, Laura ^[2];

^[1];

^[1]; Spiaggia, Giovanni ^[1]; Taladriz-Blanco, Patricia ^[1]; Barosova, Hana ^[1]; Chortarea, Savvina ^[1]; Clift, Martin J. D. ^[3];

^[4]; Sharma, Monita ^[5]; Petri-Fink, Alke ^[6];

^[1]

Univ. of Fribourg (Switzerland). Adolphe Merkle Inst.
Univ. of Fribourg (Switzerland). Adolphe Merkle Inst.; International Iberian Nanotechnology Lab. (Portugal)
Swansea Univ. Medical School (Wales)
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
PETA International Science Consortium Ltd., London (England)
Univ. of Fribourg (Switzerland). Dept. of Chemistry

The overt hazard of carbon nanotubes (CNTs) is often assessed using in vitro methods, but determining a dose–response relationship is still a challenge due to the analytical difficulty of quantifying the dose delivered to cells. An approach to accurately quantify CNT doses for submerged in vitro adherent cell culture systems using UV-VIS-near-infrared (NIR) spectroscopy is provided here. Two types of multi-walled CNTs (MWCNTs), Mitsui-7 and Nanocyl, which are dispersed in protein rich cell culture media, are studied as tested materials. Post 48 h of CNT incubation, the cellular fractions are subjected to microwave-assisted acid digestion/oxidation treatment, which eliminates biological matrix interference and improves CNT colloidal stability. The retrieved oxidized CNTs are analyzed and quantified using UV-VIS-NIR spectroscopy. In vitro imaging and quantification data in the presence of human lung epithelial cells (A549) confirm that up to 85% of Mitsui-7 and 48% for Nanocyl sediment interact (either through internalization or adherence) with cells during the 48 h of incubation. This finding is further confirmed using a sedimentation approach to estimate the delivered dose by measuring the depletion profile of the CNTs.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 1601354

Report Number(s):: PNNL-SA-150718

Journal Information:: Nanomaterials, Journal Name: Nanomaterials Journal Issue: 12 Vol. 9; ISSN 2079-4991; ISSN NANOKO

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

References (73)

Revealing the Role of Epithelial Mechanics and Macrophage Clearance during Pulmonary Epithelial Injury Recovery in the Presence of Carbon Nanotubes Septiadi, Dedy; Abdussalam, Wildan; Rodriguez-Lorenzo, Laura Advanced Materials, Vol. 30, Issue 52 https://doi.org/10.1002/adma.201806181	journal	October 2018
A Simple Spectroscopic Method for Differentiating Cellular Uptakes of Gold Nanospheres and Nanorods from Their Mixtures Cho, Eun Chul; Liu, Yang; Xia, Younan Angewandte Chemie, Vol. 122, Issue 11 https://doi.org/10.1002/ange.200906584	journal	February 2010
Quantification of Uptake and Localization of Bovine Serum Albumin-Stabilized Single-Wall Carbon Nanotubes in Different Human Cell Types Holt, Brian D.; Dahl, Kris Noel; Islam, Mohammad F. Small, Vol. 7, Issue 16 https://doi.org/10.1002/smll.201100437	journal	May 2011
UV–VIS–NIR spectroscopy study of sensitivity of single-wall carbon nanotubes to chemical processing and Van-der-Waals SWNT/SWNT interaction. Verification of the SWNT content measurements by absorption spectroscopy Ryabenko, A. G.; Dorofeeva, T. V.; Zvereva, G. I. Carbon, Vol. 42, Issue 8-9 https://doi.org/10.1016/j.carbon.2004.02.005	journal	January 2004
Chemical oxidation of multiwalled carbon nanotubes Datsyuk, V.; Kalyva, M.; Papagelis, K. Carbon, Vol. 46, Issue 6 https://doi.org/10.1016/j.carbon.2008.02.012	journal	May 2008
MWCNTs of different physicochemical properties cause similar inflammatory responses, but differences in transcriptional and histological markers of fibrosis in mouse lungs Poulsen, Sarah S.; Saber, Anne T.; Williams, Andrew Toxicology and Applied Pharmacology, Vol. 284, Issue 1 https://doi.org/10.1016/j.taap.2014.12.011	journal	April 2015
Microwave-assisted digestion procedures for biological samples with diluted nitric acid: Identification of reaction products Gonzalez, Mário H.; Souza, Gilberto B.; Oliveira, Regina V. Talanta, Vol. 79, Issue 2 https://doi.org/10.1016/j.talanta.2009.04.001	journal	July 2009
Toxicological assessment of multi-walled carbon nanotubes on A549 human lung epithelial cells Visalli, Giuseppa; Bertuccio, Maria Paola; Iannazzo, Daniela Toxicology in Vitro, Vol. 29, Issue 2 https://doi.org/10.1016/j.tiv.2014.12.004	journal	March 2015
Mouse pulmonary dose- and time course-responses induced by exposure to multi-walled carbon nanotubes Porter, Dale W.; Hubbs, Ann F.; Mercer, Robert R. Toxicology, Vol. 269, Issue 2-3 https://doi.org/10.1016/j.tox.2009.10.017	journal	March 2010
Oxide Nanoparticle Uptake in Human Lung Fibroblasts: Effects of Particle Size, Agglomeration, and Diffusion at Low Concentrations Limbach, Ludwig K.; Li, Yuchun; Grass, Robert N. Environmental Science & Technology, Vol. 39, Issue 23 https://doi.org/10.1021/es051043o	journal	December 2005
Dispersing Individual Single-Wall Carbon Nanotubes in Aqueous Surfactant Solutions below the cmc Angelikopoulos, Panagiotis; Gromov, Andrei; Leen, Ailsa The Journal of Physical Chemistry C, Vol. 114, Issue 1 https://doi.org/10.1021/jp905925r	journal	November 2009
Quantitative Techniques for Assessing and Controlling the Dispersion and Biological Effects of Multiwalled Carbon Nanotubes in Mammalian Tissue Culture Cells Wang, Xiang; Xia, Tian; Ntim, Susana Addo ACS Nano, Vol. 4, Issue 12 https://doi.org/10.1021/nn102112b	journal	November 2010
A Multi-Stakeholder Perspective on the Use of Alternative Test Strategies for Nanomaterial Safety Assessment Nel, Andre E.; Nasser, Elina; Godwin, Hilary ACS Nano, Vol. 7, Issue 8 https://doi.org/10.1021/nn4037927	journal	July 2013
Estimating the effective density of engineered nanomaterials for in vitro dosimetry DeLoid, Glen; Cohen, Joel M.; Darrah, Tom Nature Communications, Vol. 5, Issue 1 https://doi.org/10.1038/ncomms4514	journal	March 2014
A new angle on dynamic depolarized light scattering: number-averaged size distribution of nanoparticles in focus Geers, Christoph; Rodriguez-Lorenzo, Laura; Andreas Urban, Dominic Nanoscale, Vol. 8, Issue 34 https://doi.org/10.1039/c6nr03386e	journal	January 2016
Extinction coefficient of water-based multi-walled carbon nanotube nanofluids for application in direct-absorption solar collectors Lee, Seung-Hyun; Kim, Hyun Jin; Kim, Kyu Han Micro & Nano Letters, Vol. 9, Issue 10 https://doi.org/10.1049/mnl.2014.0262	journal	October 2014
Binding of blood proteins to carbon nanotubes reduces cytotoxicity Ge, C.; Du, J.; Zhao, L. Proceedings of the National Academy of Sciences, Vol. 108, Issue 41 https://doi.org/10.1073/pnas.1105270108	journal	October 2011
Effect of BSA on carbon nanotube dispersion for in vivo and in vitro studies Elgrabli, Dan; Abella-Gallart, Steve; Aguerre-Chariol, Olivier Nanotoxicology, Vol. 1, Issue 4 https://doi.org/10.1080/17435390701775136	journal	January 2007
Interactions and effects of BSA-functionalized single-walled carbon nanotubes on different cell lines Muzi, Laura; Tardani, Franco; La Mesa, Camillo Nanotechnology, Vol. 27, Issue 15 https://doi.org/10.1088/0957-4484/27/15/155704	journal	February 2016
Systematic Analysis of Multiwalled Carbon Nanotube-Induced Cellular Signaling and Gene Expression in Human Small Airway Epithelial Cells Snyder-Talkington, Brandi N.; Pacurari, Maricica; Dong, Chunlin Toxicological Sciences, Vol. 133, Issue 1 https://doi.org/10.1093/toxsci/kft019	journal	February 2013
Ultrafast Carrier Dynamics in Single-Wall Carbon Nanotubes Lauret, J-S.; Voisin, C.; Cassabois, G. Physical Review Letters, Vol. 90, Issue 5 https://doi.org/10.1103/physrevlett.90.057404	journal	February 2003
Use of Coefficient of Variation in Assessing Variability of Quantitative Assays Reed, G. F.; Lynn, F.; Meade, B. D. Clinical and Vaccine Immunology, Vol. 9, Issue 6 https://doi.org/10.1128/cdli.9.6.1235-1239.2002	journal	November 2002
Endocytosis of Multiwalled Carbon Nanotubes in Bronchial Epithelial and Mesothelial Cells Maruyama, Kayo; Haniu, Hisao; Saito, Naoto BioMed Research International, Vol. 2015 https://doi.org/10.1155/2015/793186	journal	January 2015
Distribution and persistence of pleural penetrations by multi-walled carbon nanotubes Mercer, Robert R.; Hubbs, Ann F.; Scabilloni, James F. Particle and Fibre Toxicology, Vol. 7, Issue 1 https://doi.org/10.1186/1743-8977-7-28	journal	January 2010
Advanced computational modeling for in vitro nanomaterial dosimetry DeLoid, Glen M.; Cohen, Joel M.; Pyrgiotakis, Georgios Particle and Fibre Toxicology, Vol. 12, Issue 1 https://doi.org/10.1186/s12989-015-0109-1	journal	October 2015
Quantification of the cellular dose and characterization of nanoparticle transport during in vitro testing Rischitor, Grigore; Parracino, Mariantonietta; La Spina, Rita Particle and Fibre Toxicology, Vol. 13, Issue 1 https://doi.org/10.1186/s12989-016-0157-1	journal	December 2015
On the pivotal role of dose for particle toxicology and risk assessment: exposure is a poor surrogate for delivered dose Schmid, Otmar; Cassee, Flemming R. Particle and Fibre Toxicology, Vol. 14, Issue 1 https://doi.org/10.1186/s12989-017-0233-1	journal	December 2017
ISD3: a particokinetic model for predicting the combined effects of particle sedimentation, diffusion and dissolution on cellular dosimetry for in vitro systems Thomas, Dennis G.; Smith, Jordan N.; Thrall, Brian D. Particle and Fibre Toxicology, Vol. 15, Issue 1 https://doi.org/10.1186/s12989-018-0243-7	journal	January 2018
In vitro nanoparticle dosimetry for adherent growing cell monolayers covering bottom and lateral walls Böhmert, Linda; König, Laura; Sieg, Holger Particle and Fibre Toxicology, Vol. 15, Issue 1 https://doi.org/10.1186/s12989-018-0278-9	journal	October 2018
Dispersion Behaviour of Silica Nanoparticles in Biological Media and Its Influence on Cellular Uptake Halamoda-Kenzaoui, Blanka; Ceridono, Mara; Colpo, Pascal PLOS ONE, Vol. 10, Issue 10 https://doi.org/10.1371/journal.pone.0141593	journal	October 2015
Quantitative analysis of the deposited nanoparticle dose on cell cultures by optical absorption spectroscopy Spyrogianni, Anastasia; Herrmann, Inge K.; Lucas, Miriam S. Nanomedicine, Vol. 11, Issue 19 https://doi.org/10.2217/nnm-2016-0243	journal	October 2016
Revealing the Role of Epithelial Mechanics and Macrophage Clearance during Pulmonary Epithelial Injury Recovery in the Presence of Carbon Nanotubes Septiadi, Dedy; Abdussalam, Wildan; Rodriguez-Lorenzo, Laura Advanced Materials, Vol. 30, Issue 52 https://doi.org/10.1002/adma.201806181	journal	October 2018
A Simple Spectroscopic Method for Differentiating Cellular Uptakes of Gold Nanospheres and Nanorods from Their Mixtures Cho, Eun Chul; Liu, Yang; Xia, Younan Angewandte Chemie, Vol. 122, Issue 11 https://doi.org/10.1002/ange.200906584	journal	February 2010
A Simple Spectroscopic Method for Differentiating Cellular Uptakes of Gold Nanospheres and Nanorods from Their Mixtures Cho, Eun Chul; Liu, Yang; Xia, Younan Angewandte Chemie International Edition, Vol. 49, Issue 11 https://doi.org/10.1002/anie.200906584	journal	February 2010
Quantification of Uptake and Localization of Bovine Serum Albumin-Stabilized Single-Wall Carbon Nanotubes in Different Human Cell Types Holt, Brian D.; Dahl, Kris Noel; Islam, Mohammad F. Small, Vol. 7, Issue 16 https://doi.org/10.1002/smll.201100437	journal	May 2011
Carbon Nanotubes: Advanced Topics in the Synthesis, Structure, Properties and Applications Jorio, Ado; Dresselhaus, Gene; Dresselhaus, Mildred S. Topics in Applied Physics https://doi.org/10.1007/978-3-540-72865-8	book	January 2008
UV–VIS–NIR spectroscopy study of sensitivity of single-wall carbon nanotubes to chemical processing and Van-der-Waals SWNT/SWNT interaction. Verification of the SWNT content measurements by absorption spectroscopy Ryabenko, A. G.; Dorofeeva, T. V.; Zvereva, G. I. Carbon, Vol. 42, Issue 8-9 https://doi.org/10.1016/j.carbon.2004.02.005	journal	January 2004
Chemical oxidation of multiwalled carbon nanotubes Datsyuk, V.; Kalyva, M.; Papagelis, K. Carbon, Vol. 46, Issue 6 https://doi.org/10.1016/j.carbon.2008.02.012	journal	May 2008
In vitro approaches to assess the hazard of nanomaterials Drasler, Barbara; Sayre, Phil; Steinhäuser, Klaus Günter NanoImpact, Vol. 8 https://doi.org/10.1016/j.impact.2017.08.002	journal	October 2017
MWCNTs of different physicochemical properties cause similar inflammatory responses, but differences in transcriptional and histological markers of fibrosis in mouse lungs Poulsen, Sarah S.; Saber, Anne T.; Williams, Andrew Toxicology and Applied Pharmacology, Vol. 284, Issue 1 https://doi.org/10.1016/j.taap.2014.12.011	journal	April 2015
Microwave-assisted digestion procedures for biological samples with diluted nitric acid: Identification of reaction products Gonzalez, Mário H.; Souza, Gilberto B.; Oliveira, Regina V. Talanta, Vol. 79, Issue 2 https://doi.org/10.1016/j.talanta.2009.04.001	journal	July 2009
Toxicological assessment of multi-walled carbon nanotubes on A549 human lung epithelial cells Visalli, Giuseppa; Bertuccio, Maria Paola; Iannazzo, Daniela Toxicology in Vitro, Vol. 29, Issue 2 https://doi.org/10.1016/j.tiv.2014.12.004	journal	March 2015
Mouse pulmonary dose- and time course-responses induced by exposure to multi-walled carbon nanotubes Porter, Dale W.; Hubbs, Ann F.; Mercer, Robert R. Toxicology, Vol. 269, Issue 2-3 https://doi.org/10.1016/j.tox.2009.10.017	journal	March 2010
Optical Properties and Radiation-Enhanced Evaporation of Nanofluid Fuels Containing Carbon-Based Nanostructures Gan, Yanan; Qiao, Li Energy & Fuels, Vol. 26, Issue 7 https://doi.org/10.1021/ef300493m	journal	June 2012
Oxide Nanoparticle Uptake in Human Lung Fibroblasts: Effects of Particle Size, Agglomeration, and Diffusion at Low Concentrations Limbach, Ludwig K.; Li, Yuchun; Grass, Robert N. Environmental Science & Technology, Vol. 39, Issue 23 https://doi.org/10.1021/es051043o	journal	December 2005
Dispersing Individual Single-Wall Carbon Nanotubes in Aqueous Surfactant Solutions below the cmc Angelikopoulos, Panagiotis; Gromov, Andrei; Leen, Ailsa The Journal of Physical Chemistry C, Vol. 114, Issue 1 https://doi.org/10.1021/jp905925r	journal	November 2009
Quantitative Techniques for Assessing and Controlling the Dispersion and Biological Effects of Multiwalled Carbon Nanotubes in Mammalian Tissue Culture Cells Wang, Xiang; Xia, Tian; Ntim, Susana Addo ACS Nano, Vol. 4, Issue 12 https://doi.org/10.1021/nn102112b	journal	November 2010
A Multi-Stakeholder Perspective on the Use of Alternative Test Strategies for Nanomaterial Safety Assessment Nel, Andre E.; Nasser, Elina; Godwin, Hilary ACS Nano, Vol. 7, Issue 8 https://doi.org/10.1021/nn4037927	journal	July 2013
Estimating the effective density of engineered nanomaterials for in vitro dosimetry DeLoid, Glen; Cohen, Joel M.; Darrah, Tom Nature Communications, Vol. 5, Issue 1 https://doi.org/10.1038/ncomms4514	journal	March 2014
A new angle on dynamic depolarized light scattering: number-averaged size distribution of nanoparticles in focus Geers, Christoph; Rodriguez-Lorenzo, Laura; Andreas Urban, Dominic Nanoscale, Vol. 8, Issue 34 https://doi.org/10.1039/C6NR03386E	journal	January 2016
Extinction coefficient of water-based multi-walled carbon nanotube nanofluids for application in direct-absorption solar collectors Lee, Seung-Hyun; Kim, Hyun Jin; Kim, Kyu Han Micro & Nano Letters, Vol. 9, Issue 10 https://doi.org/10.1049/mnl.2014.0262	journal	October 2014
Binding of blood proteins to carbon nanotubes reduces cytotoxicity Ge, C.; Du, J.; Zhao, L. Proceedings of the National Academy of Sciences, Vol. 108, Issue 41 https://doi.org/10.1073/pnas.1105270108	journal	October 2011
Effect of BSA on carbon nanotube dispersion for in vivo and in vitro studies Elgrabli, Dan; Abella-Gallart, Steve; Aguerre-Chariol, Olivier Nanotoxicology, Vol. 1, Issue 4 https://doi.org/10.1080/17435390701775136	journal	January 2007
Interactions and effects of BSA-functionalized single-walled carbon nanotubes on different cell lines Muzi, Laura; Tardani, Franco; La Mesa, Camillo Nanotechnology, Vol. 27, Issue 15 https://doi.org/10.1088/0957-4484/27/15/155704	journal	February 2016
Profibrotic Activity of Multiwalled Carbon Nanotubes Upon Prolonged Exposures in Different Human Lung Cell Types Chortarea, Savvina; Zerimariam, Fikad; Barosova, Hana Applied In Vitro Toxicology, Vol. 5, Issue 1 https://doi.org/10.1089/aivt.2017.0033	journal	March 2019
Systematic Analysis of Multiwalled Carbon Nanotube-Induced Cellular Signaling and Gene Expression in Human Small Airway Epithelial Cells Snyder-Talkington, Brandi N.; Pacurari, Maricica; Dong, Chunlin Toxicological Sciences, Vol. 133, Issue 1 https://doi.org/10.1093/toxsci/kft019	journal	February 2013
Ultrafast Carrier Dynamics in Single-Wall Carbon Nanotubes Lauret, J-S.; Voisin, C.; Cassabois, G. Physical Review Letters, Vol. 90, Issue 5 https://doi.org/10.1103/PhysRevLett.90.057404	journal	February 2003
Use of Coefficient of Variation in Assessing Variability of Quantitative Assays Reed, G. F.; Lynn, F.; Meade, B. D. Clinical and Vaccine Immunology, Vol. 9, Issue 6 https://doi.org/10.1128/CDLI.9.6.1235-1239.2002	journal	November 2002
Use of Coefficient of Variation in Assessing Variability of Quantitative Assays Reed, George F.; Lynn, Freyja; Meade, Bruce D. Clinical Diagnostic Laboratory Immunology, Vol. 10, Issue 6 https://doi.org/10.1128/cdli.10.6.1162.2003	journal	November 2003
Endocytosis of Multiwalled Carbon Nanotubes in Bronchial Epithelial and Mesothelial Cells Maruyama, Kayo; Haniu, Hisao; Saito, Naoto BioMed Research International, Vol. 2015 https://doi.org/10.1155/2015/793186	journal	January 2015
Distribution and persistence of pleural penetrations by multi-walled carbon nanotubes Mercer, Robert R.; Hubbs, Ann F.; Scabilloni, James F. Particle and Fibre Toxicology, Vol. 7, Issue 1 https://doi.org/10.1186/1743-8977-7-28	journal	January 2010
Pulmonary fibrotic response to aspiration of multi-walled carbon nanotubes Mercer, Robert R.; Hubbs, Ann F.; Scabilloni, James F. Particle and Fibre Toxicology, Vol. 8, Issue 1 https://doi.org/10.1186/1743-8977-8-21	journal	January 2011
Advanced computational modeling for in vitro nanomaterial dosimetry DeLoid, Glen M.; Cohen, Joel M.; Pyrgiotakis, Georgios Particle and Fibre Toxicology, Vol. 12, Issue 1 https://doi.org/10.1186/s12989-015-0109-1	journal	October 2015
Mechanisms of lung fibrosis induced by carbon nanotubes: towards an Adverse Outcome Pathway (AOP) Vietti, Giulia; Lison, Dominique; van den Brule, Sybille Particle and Fibre Toxicology, Vol. 13, Issue 1 https://doi.org/10.1186/s12989-016-0123-y	journal	December 2015
Quantification of the cellular dose and characterization of nanoparticle transport during in vitro testing Rischitor, Grigore; Parracino, Mariantonietta; La Spina, Rita Particle and Fibre Toxicology, Vol. 13, Issue 1 https://doi.org/10.1186/s12989-016-0157-1	journal	December 2015
On the pivotal role of dose for particle toxicology and risk assessment: exposure is a poor surrogate for delivered dose Schmid, Otmar; Cassee, Flemming R. Particle and Fibre Toxicology, Vol. 14, Issue 1 https://doi.org/10.1186/s12989-017-0233-1	journal	December 2017
ISD3: a particokinetic model for predicting the combined effects of particle sedimentation, diffusion and dissolution on cellular dosimetry for in vitro systems Thomas, Dennis G.; Smith, Jordan N.; Thrall, Brian D. Particle and Fibre Toxicology, Vol. 15, Issue 1 https://doi.org/10.1186/s12989-018-0243-7	journal	January 2018
In vitro nanoparticle dosimetry for adherent growing cell monolayers covering bottom and lateral walls Böhmert, Linda; König, Laura; Sieg, Holger Particle and Fibre Toxicology, Vol. 15, Issue 1 https://doi.org/10.1186/s12989-018-0278-9	journal	October 2018
Dispersion Behaviour of Silica Nanoparticles in Biological Media and Its Influence on Cellular Uptake Halamoda-Kenzaoui, Blanka; Ceridono, Mara; Colpo, Pascal PLOS ONE, Vol. 10, Issue 10 https://doi.org/10.1371/journal.pone.0141593	journal	October 2015
Quantitative analysis of the deposited nanoparticle dose on cell cultures by optical absorption spectroscopy Spyrogianni, Anastasia; Herrmann, Inge K.; Lucas, Miriam S. Nanomedicine, Vol. 11, Issue 19 https://doi.org/10.2217/nnm-2016-0243	journal	October 2016
A review on protein functionalized carbon nanotubes Nagaraju, Kathyayini; Reddy, Roopa; Reddy, Narendra Journal of Applied Biomaterials & Functional Materials, Vol. 13, Issue 4 https://doi.org/10.5301/jabfm.5000231	journal	January 2015
ISD3: a particokinetic model for predicting the combined effects of particle sedimentation, diffusion and dissolution on cellular dosimetry for in vitro systems Thomas, Dennis; Smith, Jordan; Thrall, Brian Figshare https://doi.org/10.6084/m9.figshare.c.3986475.v1	collection	January 2018
In vitro nanoparticle dosimetry for adherent growing cell monolayers covering bottom and lateral walls Böhmert, Linda; König, Laura; Sieg, Holger Figshare https://doi.org/10.6084/m9.figshare.c.4285058	collection	January 2018

Similar Records

ROS and NF-{kappa}B are involved in upregulation of IL-8 in A549 cells exposed to multi-walled carbon nanotubes

Journal Article · 2009 · Biochemical and Biophysical Research Communications · OSTI ID:21255876

Yihui, Wu; Zhenqing, Hou

TU-H-CAMPUS-TeP3-01: Gold Nanoparticle-Enhanced Radiation Therapy in In Vitro A549 Lung Carcinoma: Studies in Both Traditional Monolayer and Three Dimensional Cell Culture Models

Journal Article · 2016 · Medical Physics · OSTI ID:22654077

Oumano, M; Ngwa, W; Celli, J; +4 more

Nitrative DNA damage induced by multi-walled carbon nanotube via endocytosis in human lung epithelial cells

Journal Article · 2012 · Toxicology and Applied Pharmacology · OSTI ID:22215297

Guo, Feiye; Ma, Ning; Horibe, Yoshiteru; +3 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
UV-VIS-NIR spectroscopy
adherent cell cultures
carbon nanotubes
depletion
quantification
sedimentation

Title: Quantification of Carbon Nanotube Doses in Adherent Cell Culture Assays Using UV-VIS-NIR Spectroscopy

Citation Formats

References (73)

Similar Records

Related Subjects