The essential effector SCRE1 in Ustilaginoidea virens suppresses rice immunity via a small peptide region

Zhang, Nan; Yang, Jiyun; Fang, Anfei; Wang, Jiyang; Li, Dayong; Li, Yuejiao; Wang, Shanzhi; Cui, Fuhao; Yu, Junjie; Liu, Yongfeng; Peng, You‐Liang; Sun, Wenxian

doi:10.1111/mpp.12894

Title: The essential effector SCRE1 in Ustilaginoidea virens suppresses rice immunity via a small peptide region

Journal Article · 21 February 2020 · Molecular Plant Pathology

DOI: https://doi.org/10.1111/mpp.12894 · OSTI ID:1601306

Zhang, Nan ^[1]; Yang, Jiyun ^[1]; Fang, Anfei ^[1]; Wang, Jiyang ^[1]; Li, Dayong ^[2]; Li, Yuejiao ^[1]; Wang, Shanzhi ^[1]; Cui, Fuhao ^[1]; Yu, Junjie ^[3]; Liu, Yongfeng ^[3];

^[4];

^[5]

Department of Plant Pathology and the Ministry of Agriculture Key Laboratory of Pest Monitoring and Green Management China Agricultural University Beijing 100193 China
College of Plant Protection Jilin Agricultural University Changchun 130118 China
Institute of Plant Protection Jiangsu Academy of Agricultural Sciences Nanjing, Jiangsu 210014 China
Department of Plant Pathology and the Ministry of Agriculture Key Laboratory of Pest Monitoring and Green Management China Agricultural University Beijing 100193 China, State Key Laboratory of Agricultural Biotechnology China Agricultural University Beijing 100193 China
Department of Plant Pathology and the Ministry of Agriculture Key Laboratory of Pest Monitoring and Green Management China Agricultural University Beijing 100193 China, College of Plant Protection Jilin Agricultural University Changchun 130118 China

Summary The biotrophic fungal pathogen Ustilaginoidea virens causes rice false smut, a newly emerging plant disease that has become epidemic worldwide in recent years. The U. virens genome encodes many putative effector proteins that, based on the study of other pathosystems, could play an essential role in fungal virulence. However, few studies have been reported on virulence functions of individual U. virens effectors. Here, we report our identification and characterization of the secreted cysteine‐rich protein SCRE1, which is an essential virulence effector in U. virens . When SCRE1 was heterologously expressed in Magnaporthe oryzae , the protein was secreted and translocated into plant cells during infection. SCRE1 suppresses the immunity‐associated hypersensitive response in the nonhost plant Nicotiana benthamiana . Induced expression of SCRE1 in rice also inhibits pattern‐triggered immunity and enhances disease susceptibility to rice bacterial and fungal pathogens. The immunosuppressive activity is localized to a small peptide region that contains an important ‘cysteine‐proline‐alanine‐arginine‐serine’ motif. Furthermore, the scre1 knockout mutant generated using the CRISPR/Cas9 system is attenuated in U. virens virulence to rice, which is greatly complemented by the full‐length SCRE1 gene. Collectively, this study indicates that the effector SCRE1 is able to inhibit host immunity and is required for full virulence of U. virens .

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Sponsoring Organization:: USDOE

OSTI ID:: 1601306

Journal Information:: Molecular Plant Pathology, Journal Name: Molecular Plant Pathology Journal Issue: 4 Vol. 21; ISSN 1464-6722

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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