Ultrastructural and SINS analysis of the cell wall integrity response of Aspergillus nidulans to the absence of galactofuranose
Abstract
With lethal opportunistic fungal infections on the rise, it is imperative to explore new methods to examine virulence mechanisms. The fungal cell wall is crucial for both the virulence and viability of Aspergillus nidulans. One wall component, Galf, has been shown to contribute to important fungal processes, integrity of the cell wall and pathogenesis. Here in this paper, we explore gene deletion strains lacking the penultimate enzyme in Galf biosynthesis (ugmAΔ) and the protein that transports Galf for incorporation into the cell wall (ugtAΔ). In applying gene deletion technology to the problem of cell wall integrity, we have employed multiple micro- and nano-scale imaging tools, including confocal fluorescence microscopy, electron microscopy, X-Ray fluorescence and atomic force microscopy. Atomic force microscopy allows quantification of ultrastructural cell wall architecture while near-field infrared spectroscopy provides spatially resolved chemical signatures, both at the nanoscale. Here, for the first time, we demonstrate correlative data collection with these two emerging modalities for the multiplexed in situ study of the nanoscale architecture and chemical composition of fungal cell walls.
- Authors:
-
[1];
[1];
[1];
[2];
[5];
[1]
- Univ. of Manitoba, Winnipeg (Canada)
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Advanced Light Source (ALS)
- Neaspec GmbH, Martinsried (Germany)
- Univ. of Saskatchewan, Saskatoon, SK (Canada)
- Univ. of Regina, Regina (Canada)
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC); Natural Sciences and Engineering Research Council of Canada (NSERC); University of Manitoba
- OSTI Identifier:
- 1601196
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Analyst
- Additional Journal Information:
- Journal Volume: 144; Journal Issue: 3; Journal ID: ISSN 0003-2654
- Publisher:
- Royal Society of Chemistry
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Bakir, Görkem, Girouard, Benoit E., Johns, Robert W., Findlay, Catherine R. -J., Bechtel, Hans A., Eisele, Max, Kaminskyj, Susan G. W., Dahms, Tanya E. S., and Gough, Kathleen M. Ultrastructural and SINS analysis of the cell wall integrity response of Aspergillus nidulans to the absence of galactofuranose. United States: N. p., 2018.
Web. doi:10.1039/c8an01591k.
Bakir, Görkem, Girouard, Benoit E., Johns, Robert W., Findlay, Catherine R. -J., Bechtel, Hans A., Eisele, Max, Kaminskyj, Susan G. W., Dahms, Tanya E. S., & Gough, Kathleen M. Ultrastructural and SINS analysis of the cell wall integrity response of Aspergillus nidulans to the absence of galactofuranose. United States. https://doi.org/10.1039/c8an01591k
Bakir, Görkem, Girouard, Benoit E., Johns, Robert W., Findlay, Catherine R. -J., Bechtel, Hans A., Eisele, Max, Kaminskyj, Susan G. W., Dahms, Tanya E. S., and Gough, Kathleen M. Thu .
"Ultrastructural and SINS analysis of the cell wall integrity response of Aspergillus nidulans to the absence of galactofuranose". United States. https://doi.org/10.1039/c8an01591k. https://www.osti.gov/servlets/purl/1601196.
@article{osti_1601196,
title = {Ultrastructural and SINS analysis of the cell wall integrity response of Aspergillus nidulans to the absence of galactofuranose},
author = {Bakir, Görkem and Girouard, Benoit E. and Johns, Robert W. and Findlay, Catherine R. -J. and Bechtel, Hans A. and Eisele, Max and Kaminskyj, Susan G. W. and Dahms, Tanya E. S. and Gough, Kathleen M.},
abstractNote = {With lethal opportunistic fungal infections on the rise, it is imperative to explore new methods to examine virulence mechanisms. The fungal cell wall is crucial for both the virulence and viability of Aspergillus nidulans. One wall component, Galf, has been shown to contribute to important fungal processes, integrity of the cell wall and pathogenesis. Here in this paper, we explore gene deletion strains lacking the penultimate enzyme in Galf biosynthesis (ugmAΔ) and the protein that transports Galf for incorporation into the cell wall (ugtAΔ). In applying gene deletion technology to the problem of cell wall integrity, we have employed multiple micro- and nano-scale imaging tools, including confocal fluorescence microscopy, electron microscopy, X-Ray fluorescence and atomic force microscopy. Atomic force microscopy allows quantification of ultrastructural cell wall architecture while near-field infrared spectroscopy provides spatially resolved chemical signatures, both at the nanoscale. Here, for the first time, we demonstrate correlative data collection with these two emerging modalities for the multiplexed in situ study of the nanoscale architecture and chemical composition of fungal cell walls.},
doi = {10.1039/c8an01591k},
journal = {Analyst},
number = 3,
volume = 144,
place = {United States},
year = {Thu Nov 01 00:00:00 EDT 2018},
month = {Thu Nov 01 00:00:00 EDT 2018}
}
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