Ancient origins of allosteric activation in a Ser-Thr kinase
Abstract
A myriad of cellular events are regulated by allostery; therefore, evolution of this process is of fundamental interest. Here, we use ancestral sequence reconstruction to resurrect ancestors of two colocalizing proteins, Aurora A kinase and its allosteric activator TPX2 (targeting protein for Xklp2), to experimentally characterize the evolutionary path of allosteric activation. Autophosphorylation of the activation loop is the most ancient activation mechanism; it is fully developed in the oldest kinase ancestor and has remained stable over 1 billion years of evolution. As the microtubule-associated protein TPX2 appeared, efficient kinase binding to TPX2 evolved, likely owing to increased fitness by virtue of colocalization. Subsequently, TPX2-mediated allosteric kinase regulation gradually evolved. Surprisingly, evolution of this regulation is encoded in the kinase and did not arise by a dominating mechanism of coevolution.
- Authors:
-
[1];
[1];
[1];
[1];
[1];
[1]
- Department of Biochemistry, Brandeis University, Waltham, MA 02454, USA., Howard Hughes Medical Institute, Brandeis University, Waltham, MA 02454, USA.
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1600873
- Grant/Contract Number:
- FG02-05ER15699
- Resource Type:
- Published Article
- Journal Name:
- Science
- Additional Journal Information:
- Journal Name: Science Journal Volume: 367 Journal Issue: 6480; Journal ID: ISSN 0036-8075
- Publisher:
- American Association for the Advancement of Science (AAAS)
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Hadzipasic, Adelajda, Wilson, Christopher, Nguyen, Vy, Kern, Nadja, Kim, Chansik, Pitsawong, Warintra, Villali, Janice, Zheng, Yuejiao, and Kern, Dorothee. Ancient origins of allosteric activation in a Ser-Thr kinase. United States: N. p., 2020.
Web. doi:10.1126/science.aay9959.
Hadzipasic, Adelajda, Wilson, Christopher, Nguyen, Vy, Kern, Nadja, Kim, Chansik, Pitsawong, Warintra, Villali, Janice, Zheng, Yuejiao, & Kern, Dorothee. Ancient origins of allosteric activation in a Ser-Thr kinase. United States. https://doi.org/10.1126/science.aay9959
Hadzipasic, Adelajda, Wilson, Christopher, Nguyen, Vy, Kern, Nadja, Kim, Chansik, Pitsawong, Warintra, Villali, Janice, Zheng, Yuejiao, and Kern, Dorothee. Thu .
"Ancient origins of allosteric activation in a Ser-Thr kinase". United States. https://doi.org/10.1126/science.aay9959.
@article{osti_1600873,
title = {Ancient origins of allosteric activation in a Ser-Thr kinase},
author = {Hadzipasic, Adelajda and Wilson, Christopher and Nguyen, Vy and Kern, Nadja and Kim, Chansik and Pitsawong, Warintra and Villali, Janice and Zheng, Yuejiao and Kern, Dorothee},
abstractNote = {A myriad of cellular events are regulated by allostery; therefore, evolution of this process is of fundamental interest. Here, we use ancestral sequence reconstruction to resurrect ancestors of two colocalizing proteins, Aurora A kinase and its allosteric activator TPX2 (targeting protein for Xklp2), to experimentally characterize the evolutionary path of allosteric activation. Autophosphorylation of the activation loop is the most ancient activation mechanism; it is fully developed in the oldest kinase ancestor and has remained stable over 1 billion years of evolution. As the microtubule-associated protein TPX2 appeared, efficient kinase binding to TPX2 evolved, likely owing to increased fitness by virtue of colocalization. Subsequently, TPX2-mediated allosteric kinase regulation gradually evolved. Surprisingly, evolution of this regulation is encoded in the kinase and did not arise by a dominating mechanism of coevolution.},
doi = {10.1126/science.aay9959},
journal = {Science},
number = 6480,
volume = 367,
place = {United States},
year = {Thu Feb 20 00:00:00 EST 2020},
month = {Thu Feb 20 00:00:00 EST 2020}
}
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https://doi.org/10.1126/science.aay9959
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