The C-Terminal Domains SnRK2 Box and ABA Box Have a Role in Sugarcane SnRK2s Auto-Activation and Activity

Righetto, Germanna Lima; Sriranganadane, Dev; Halabelian, Levon; Chiodi, Carla G.; Elkins, Jonathan M.; Massirer, Katlin B.; Gileadi, Opher; Menossi, Marcelo; Couñago, Rafael M.

doi:10.3389/fpls.2019.01105

Title: The C-Terminal Domains SnRK2 Box and ABA Box Have a Role in Sugarcane SnRK2s Auto-Activation and Activity

Journal Article · 17 September 2019 · Frontiers in Plant Science

DOI: https://doi.org/10.3389/fpls.2019.01105 · OSTI ID:1600826

Righetto, Germanna Lima ^[1]; Sriranganadane, Dev ^[2]; Halabelian, Levon ^[3]; Chiodi, Carla G. ^[2]; Elkins, Jonathan M. ^[4]; Massirer, Katlin B. ^[2]; Gileadi, Opher ^[5]; Menossi, Marcelo ^[1]; Couñago, Rafael M. ^[2]

State Univ. of Campinas (Brazil)
Univ. Estadual de Campinas (UNICAMP) (Brazil)
MaRS Center, Toronto, ON (Canada)
Univ. Estadual de Campinas (UNICAMP) (Brazil); Univ. of Oxford (United Kingdom)
Univ. of Oxford (United Kingdom)

Resistance to drought stress is fundamental to plant survival and development. Abscisic acid (ABA) is one of the major hormones involved in different types of abiotic and biotic stress responses. ABA intracellular signaling has been extensively explored in Arabidopsis thaliana and occurs via a phosphorylation cascade mediated by three related protein kinases, denominated SnRK2s (SNF1-related protein kinases). However, the role of ABA signaling and the biochemistry of SnRK2 in crop plants remains underexplored. Considering the importance of the ABA hormone in abiotic stress tolerance, here we investigated the regulatory mechanism of sugarcane SnRK2s—known as stress/ABAactivated protein kinases (SAPKs). The crystal structure of ScSAPK10 revealed the characteristic SnRK2 family architecture, in which the regulatory SnRK2 box interacts with the kinase domain αC helix. To study sugarcane SnRK2 regulation, we produced a series of mutants for the protein regulatory domains SnRK2 box and ABA box. Mutations in ScSAPK8 SnRK2 box aimed at perturbing its interaction with the protein kinase domain reduced protein kinase activity in vitro. On the other hand, mutations to ScSAPK ABA box did not impact protein kinase activity but did alter the protein autophosphorylation pattern. Taken together, our results demonstrate that both SnRK2 and ABA boxes might play a role in sugarcane SnRK2 function.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1600826

Journal Information:: Frontiers in Plant Science, Vol. 10, Issue 09, 2019; ISSN 1664-462X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 3 works

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References (87)

High-throughput production of human proteins for crystallization: The SGC experience Savitsky, Pavel; Bray, James; Cooper, Christopher D. O. Journal of Structural Biology, Vol. 172, Issue 1 https://doi.org/10.1016/j.jsb.2010.06.008	journal	October 2010
A gate–latch–lock mechanism for hormone signalling by abscisic acid receptors Melcher, Karsten; Ng, Ley-Moy; Zhou, X. Edward Nature, Vol. 462, Issue 7273 https://doi.org/10.1038/nature08613	journal	November 2009
Overview of the CCP 4 suite and current developments Winn, Martyn D.; Ballard, Charles C.; Cowtan, Kevin D. Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4 https://doi.org/10.1107/S0907444910045749	journal	March 2011
ZmOST1 mediates abscisic acid regulation of guard cell ion channels and drought stress responses Wu, Qiqi; Wang, Mei; Shen, Jianlin Journal of Integrative Plant Biology, Vol. 61, Issue 4 https://doi.org/10.1111/jipb.12714	journal	November 2018
The EMBL-EBI search and sequence analysis tools APIs in 2019 Madeira, Fábio; Park, Young mi; Lee, Joon Nucleic Acids Research, Vol. 47, Issue W1 https://doi.org/10.1093/nar/gkz268	journal	April 2019
Abscisic Acid Inhibits Type 2C Protein Phosphatases via the PYR/PYL Family of START Proteins Park, S. -Y.; Fung, P.; Nishimura, N. Science https://doi.org/10.1126/science.1173041	journal	April 2009
Arabidopsis OST1 Protein Kinase Mediates the Regulation of Stomatal Aperture by Abscisic Acid and Acts Upstream of Reactive Oxygen Species Production Mustilli, Anna-Chiara; Merlot, Sylvain; Vavasseur, Alain The Plant Cell, Vol. 14, Issue 12 https://doi.org/10.1105/tpc.007906	journal	November 2002
Identification and Bioinformatics Analysis of SnRK2 and CIPK Family Genes in Sorghum Li, Li-bin; Zhang, Yi-rong; Liu, Kai-chang Agricultural Sciences in China, Vol. 9, Issue 1 https://doi.org/10.1016/S1671-2927(09)60063-8	journal	January 2010
The PRIDE database and related tools and resources in 2019: improving support for quantification data Perez-Riverol, Yasset; Csordas, Attila; Bai, Jingwen Nucleic Acids Research, Vol. 47, Issue D1 https://doi.org/10.1093/nar/gky1106	journal	November 2018
ABA-Activated SnRK2 Protein Kinase is Required for Dehydration Stress Signaling in Arabidopsis Yoshida, Riichiro; Hobo, Tokunori; Ichimura, Kazuya Plant and Cell Physiology, Vol. 43, Issue 12 https://doi.org/10.1093/pcp/pcf188	journal	December 2002
Structural basis of abscisic acid signalling Miyazono, Ken-ichi; Miyakawa, Takuya; Sawano, Yoriko Nature, Vol. 462, Issue 7273 https://doi.org/10.1038/nature08583	journal	October 2009
Structure of Ligand-Bound Intermediates of Crop ABA Receptors Highlights PP2C as Necessary ABA Co-receptor Moreno-Alvero, María; Yunta, Cristina; Gonzalez-Guzman, Miguel Molecular Plant, Vol. 10, Issue 9 https://doi.org/10.1016/j.molp.2017.07.004	journal	September 2017
Protein Phosphatases 2C Regulate the Activation of the Snf1-Related Kinase OST1 by Abscisic Acid in Arabidopsis Vlad, Florina; Rubio, Silvia; Rodrigues, Americo The Plant Cell, Vol. 21, Issue 10 https://doi.org/10.1105/tpc.109.069179	journal	October 2009
Type 2C protein phosphatases directly regulate abscisic acid-activated protein kinases in Arabidopsis Umezawa, T.; Sugiyama, N.; Mizoguchi, M. Proceedings of the National Academy of Sciences, Vol. 106, Issue 41 https://doi.org/10.1073/pnas.0907095106	journal	September 2009
PHENIX : building new software for automated crystallographic structure determination Adams, Paul D.; Grosse-Kunstleve, Ralf W.; Hung, Li-Wei Acta Crystallographica Section D Biological Crystallography, Vol. 58, Issue 11 https://doi.org/10.1107/S0907444902016657	journal	October 2002
Structural basis for basal activity and autoactivation of abscisic acid (ABA) signaling SnRK2 kinases Ng, L. -M.; Soon, F. -F.; Zhou, X. E. Proceedings of the National Academy of Sciences, Vol. 108, Issue 52 https://doi.org/10.1073/pnas.1118651109	journal	December 2011
XDS Kabsch, Wolfgang Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2 https://doi.org/10.1107/S0907444909047337	journal	January 2010
Differential Activation of the Rice Sucrose Nonfermenting1–Related Protein Kinase2 Family by Hyperosmotic Stress and Abscisic Acid Kobayashi, Yuhko; Yamamoto, Shuhei; Minami, Hideyuki The Plant Cell, Vol. 16, Issue 5 https://doi.org/10.1105/tpc.019943	journal	April 2004
In vitro reconstitution of an abscisic acid signalling pathway Fujii, Hiroaki; Chinnusamy, Viswanathan; Rodrigues, Americo Nature, Vol. 462, Issue 7273 https://doi.org/10.1038/nature08599	journal	November 2009
SMART, a simple modular architecture research tool: Identification of signaling domains Schultz, J.; Milpetz, F.; Bork, P. Proceedings of the National Academy of Sciences, Vol. 95, Issue 11 https://doi.org/10.1073/pnas.95.11.5857	journal	May 1998
Identification of Two Protein Kinases Required for Abscisic Acid Regulation of Seed Germination, Root Growth, and Gene Expression in Arabidopsis Fujii, Hiroaki; Verslues, Paul E.; Zhu, Jian-Kang The Plant Cell, Vol. 19, Issue 2 https://doi.org/10.1105/tpc.106.048538	journal	February 2007
Molecular Mimicry Regulates ABA Signaling by SnRK2 Kinases and PP2C Phosphatases Soon, Fen-Fen; Ng, Ley-Moy; Zhou, X. Edward Science, Vol. 335, Issue 6064 https://doi.org/10.1126/science.1215106	journal	November 2011
The Structure of Arabidopsis thaliana OST1 Provides Insights into the Kinase Regulation Mechanism in Response to Osmotic Stress Yunta, Cristina; Martínez-Ripoll, Martín; Zhu, Jian-Kang Journal of Molecular Biology, Vol. 414, Issue 1 https://doi.org/10.1016/j.jmb.2011.09.041	journal	November 2011
The Pfam protein families database: towards a more sustainable future Finn, Robert D.; Coggill, Penelope; Eberhardt, Ruth Y. Nucleic Acids Research, Vol. 44, Issue D1 https://doi.org/10.1093/nar/gkv1344	journal	December 2015
Structural insights into the mechanism of abscisic acid signaling by PYL proteins Yin, Ping; Fan, He; Hao, Qi Nature Structural & Molecular Biology, Vol. 16, Issue 12 https://doi.org/10.1038/nsmb.1730	journal	November 2009
MolProbity : all-atom structure validation for macromolecular crystallography Chen, Vincent B.; Arendall, W. Bryan; Headd, Jeffrey J. Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 1 https://doi.org/10.1107/S0907444909042073	journal	December 2009
Arabidopsis mutant deficient in 3 abscisic acid-activated protein kinases reveals critical roles in growth, reproduction, and stress Fujii, H.; Zhu, J. -K. Proceedings of the National Academy of Sciences, Vol. 106, Issue 20 https://doi.org/10.1073/pnas.0903144106	journal	May 2009
The rapid generation of mutation data matrices from protein sequences Jones, David T.; Taylor, William R.; Thornton, Janet M. Bioinformatics, Vol. 8, Issue 3 https://doi.org/10.1093/bioinformatics/8.3.275	journal	January 1992
Different phosphorylation mechanisms are involved in the activation of sucrose non-fermenting 1 related protein kinases 2 by osmotic stresses and abscisic acid Boudsocq, Marie; Droillard, Marie-Jo; Barbier-Brygoo, Hélène Plant Molecular Biology, Vol. 63, Issue 4 https://doi.org/10.1007/s11103-006-9103-1	journal	November 2006
Features and development of Coot Emsley, P.; Lohkamp, B.; Scott, W. G. Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4 https://doi.org/10.1107/S0907444910007493	journal	March 2010
Overexpression of a Common Wheat Gene TaSnRK2.8 Enhances Tolerance to Drought, Salt and Low Temperature in Arabidopsis Zhang, Hongying; Mao, Xinguo; Wang, Chengshe PLoS ONE, Vol. 5, Issue 12 https://doi.org/10.1371/journal.pone.0016041	journal	December 2010
REFMAC 5 for the refinement of macromolecular crystal structures Murshudov, Garib N.; Skubák, Pavol; Lebedev, Andrey A. Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4 https://doi.org/10.1107/S0907444911001314	journal	March 2011
Mechanism of CDK activation revealed by the structure of a cyclinA-CDK2 complex Jeffrey, Philip D.; Russo, Alicia A.; Polyak, Kornelia Nature, Vol. 376, Issue 6538 https://doi.org/10.1038/376313a0	journal	July 1995
Three Arabidopsis SnRK2 Protein Kinases, SRK2D/SnRK2.2, SRK2E/SnRK2.6/OST1 and SRK2I/SnRK2.3, Involved in ABA Signaling are Essential for the Control of Seed Development and Dormancy Nakashima, Kazuo; Fujita, Yasunari; Kanamori, Norihito Plant and Cell Physiology, Vol. 50, Issue 7 https://doi.org/10.1093/pcp/pcp083	journal	June 2009
Regulators of PP2C Phosphatase Activity Function as Abscisic Acid Sensors Ma, Y.; Szostkiewicz, I.; Korte, A. Science https://doi.org/10.1126/science.1172408	journal	April 2009
MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets Kumar, Sudhir; Stecher, Glen; Tamura, Koichiro Molecular Biology and Evolution, Vol. 33, Issue 7 https://doi.org/10.1093/molbev/msw054	journal	March 2016
Identification of Features Regulating OST1 Kinase Activity and OST1 Function in Guard Cells Belin, Christophe; de Franco, Pierre-Olivier; Bourbousse, Clara Plant Physiology, Vol. 141, Issue 4 https://doi.org/10.1104/pp.106.079327	journal	June 2006
Regulation of Protein Kinases Nolen, Brad; Taylor, Susan; Ghosh, Gourisankar Molecular Cell, Vol. 15, Issue 5 https://doi.org/10.1016/j.molcel.2004.08.024	journal	September 2004
Three SnRK2 Protein Kinases are the Main Positive Regulators of Abscisic Acid Signaling in Response to Water Stress in Arabidopsis Fujita, Yasunari; Nakashima, Kazuo; Yoshida, Takuya Plant and Cell Physiology, Vol. 50, Issue 12 https://doi.org/10.1093/pcp/pcp147	journal	September 2009
GSK3-like kinases positively modulate abscisic acid signaling through phosphorylating subgroup III SnRK2s in Arabidopsis Cai, Zhenying; Liu, Jingjing; Wang, Haijiao Proceedings of the National Academy of Sciences, Vol. 111, Issue 26 https://doi.org/10.1073/pnas.1316717111	journal	June 2014
Molecular Basis of the Core Regulatory Network in ABA Responses: Sensing, Signaling and Transport Umezawa, T.; Nakashima, K.; Miyakawa, T. Plant and Cell Physiology, Vol. 51, Issue 11 https://doi.org/10.1093/pcp/pcq156	journal	October 2010
Genome-wide identification and characterization of SnRK2 gene family in cotton (Gossypium hirsutum L.) Liu, Zhao; Ge, Xiaoyang; Yang, Zuoren BMC Genetics, Vol. 18, Issue 1 https://doi.org/10.1186/s12863-017-0517-3	journal	June 2017
SnRK2 Protein Kinases—Key Regulators of Plant Response to Abiotic Stresses Kulik, Anna; Wawer, Izabela; Krzywińska, Ewa OMICS: A Journal of Integrative Biology, Vol. 15, Issue 12 https://doi.org/10.1089/omi.2011.0091	journal	December 2011
The Maize OST1 Kinase Homolog Phosphorylates and Regulates the Maize SNAC1-Type Transcription Factor Vilela, Belmiro; Moreno-Cortés, Alicia; Rabissi, Agnese PLoS ONE, Vol. 8, Issue 2 https://doi.org/10.1371/journal.pone.0058105	journal	February 2013
The nuts and bolts of AGC protein kinases Pearce, Laura R.; Komander, David; Alessi, Dario R. Nature Reviews Molecular Cell Biology, Vol. 11, Issue 1 https://doi.org/10.1038/nrm2822	journal	January 2010
Clustal Omega, Accurate Alignment of Very Large Numbers of Sequences Sievers, Fabian; Higgins, Desmond G. Multiple Sequence Alignment Methods https://doi.org/10.1007/978-1-62703-646-7_6	book	January 2013
Cloning and characterization of the SnRK2 gene family from Zea mays Huai, Junling; Wang, Meng; He, Junguang Plant Cell Reports, Vol. 27, Issue 12 https://doi.org/10.1007/s00299-008-0608-8	journal	September 2008
Analysis and Functional Annotation of an Expressed Sequence Tag Collection for Tropical Crop Sugarcane Vettore, A. L. Genome Research, Vol. 13, Issue 12 https://doi.org/10.1101/gr.1532103	journal	December 2003
Cloning and characterization of the SnRK2 gene family from Zea mays Huai, Junling; Wang, Meng; He, Junguang Plant Cell Reports, Vol. 27, Issue 12 https://doi.org/10.1007/s00299-008-0608-8	journal	September 2008
The Structure of Arabidopsis thaliana OST1 Provides Insights into the Kinase Regulation Mechanism in Response to Osmotic Stress Yunta, Cristina; Martínez-Ripoll, Martín; Zhu, Jian-Kang Journal of Molecular Biology, Vol. 414, Issue 1 https://doi.org/10.1016/j.jmb.2011.09.041	journal	November 2011
Regulation of Protein Kinases Nolen, Brad; Taylor, Susan; Ghosh, Gourisankar Molecular Cell, Vol. 15, Issue 5 https://doi.org/10.1016/j.molcel.2004.08.024	journal	September 2004
Structure of Ligand-Bound Intermediates of Crop ABA Receptors Highlights PP2C as Necessary ABA Co-receptor Moreno-Alvero, María; Yunta, Cristina; Gonzalez-Guzman, Miguel Molecular Plant, Vol. 10, Issue 9 https://doi.org/10.1016/j.molp.2017.07.004	journal	September 2017
Mechanism of CDK activation revealed by the structure of a cyclinA-CDK2 complex Jeffrey, Philip D.; Russo, Alicia A.; Polyak, Kornelia Nature, Vol. 376, Issue 6538 https://doi.org/10.1038/376313a0	journal	July 1995
Structural basis of abscisic acid signalling Miyazono, Ken-ichi; Miyakawa, Takuya; Sawano, Yoriko Nature, Vol. 462, Issue 7273 https://doi.org/10.1038/nature08583	journal	October 2009
In vitro reconstitution of an abscisic acid signalling pathway Fujii, Hiroaki; Chinnusamy, Viswanathan; Rodrigues, Americo Nature, Vol. 462, Issue 7273 https://doi.org/10.1038/nature08599	journal	November 2009
A gate–latch–lock mechanism for hormone signalling by abscisic acid receptors Melcher, Karsten; Ng, Ley-Moy; Zhou, X. Edward Nature, Vol. 462, Issue 7273 https://doi.org/10.1038/nature08613	journal	November 2009
The nuts and bolts of AGC protein kinases Pearce, Laura R.; Komander, David; Alessi, Dario R. Nature Reviews Molecular Cell Biology, Vol. 11, Issue 1 https://doi.org/10.1038/nrm2822	journal	January 2010
Structural insights into the mechanism of abscisic acid signaling by PYL proteins Yin, Ping; Fan, He; Hao, Qi Nature Structural & Molecular Biology, Vol. 16, Issue 12 https://doi.org/10.1038/nsmb.1730	journal	November 2009
MX2-mediated innate immunity against HIV-1 is regulated by serine phosphorylation Betancor, Gilberto; Jimenez-Guardeño, Jose M.; Lynham, Steven Nature Microbiology, Vol. 6, Issue 8 https://doi.org/10.1038/s41564-021-00937-5	journal	July 2021
Host ANP32A mediates the assembly of the influenza virus replicase Carrique, Loïc; Fan, Haitian; Walker, Alexander P. Nature, Vol. 587, Issue 7835 https://doi.org/10.1038/s41586-020-2927-z	journal	November 2020
Molecular Characterization and Co-expression Analysis of the SnRK2 Gene Family in Sugarcane (Saccharum officinarum L.) Li, Changning; Nong, Qian; Xie, Jinlan Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/s41598-017-16152-4	journal	December 2017
Characterization and phylogenetic analysis of the complete mitochondrial genome of the pathogenic fungus Ilyonectria destructans Androsiuk, Piotr; Okorski, Adam; Paukszto, Łukasz Scientific Reports, Vol. 12, Issue 1 https://doi.org/10.1038/s41598-022-05428-z	journal	February 2022
Arabidopsis mutant deficient in 3 abscisic acid-activated protein kinases reveals critical roles in growth, reproduction, and stress Fujii, H.; Zhu, J. -K. Proceedings of the National Academy of Sciences, Vol. 106, Issue 20 https://doi.org/10.1073/pnas.0903144106	journal	May 2009
Type 2C protein phosphatases directly regulate abscisic acid-activated protein kinases in Arabidopsis Umezawa, T.; Sugiyama, N.; Mizoguchi, M. Proceedings of the National Academy of Sciences, Vol. 106, Issue 41 https://doi.org/10.1073/pnas.0907095106	journal	September 2009
Structural basis for basal activity and autoactivation of abscisic acid (ABA) signaling SnRK2 kinases Ng, L. -M.; Soon, F. -F.; Zhou, X. E. Proceedings of the National Academy of Sciences, Vol. 108, Issue 52 https://doi.org/10.1073/pnas.1118651109	journal	December 2011
GSK3-like kinases positively modulate abscisic acid signaling through phosphorylating subgroup III SnRK2s in Arabidopsis Cai, Zhenying; Liu, Jingjing; Wang, Haijiao Proceedings of the National Academy of Sciences, Vol. 111, Issue 26 https://doi.org/10.1073/pnas.1316717111	journal	June 2014
SMART, a simple modular architecture research tool: Identification of signaling domains Schultz, J.; Milpetz, F.; Bork, P. Proceedings of the National Academy of Sciences, Vol. 95, Issue 11 https://doi.org/10.1073/pnas.95.11.5857	journal	May 1998
Identification of Nine Sucrose Nonfermenting 1-related Protein Kinases 2 Activated by Hyperosmotic and Saline Stresses in Arabidopsis thaliana Boudsocq, M.; Barbier-Brygoo, H.; Lauriere, C. Journal of Biological Chemistry, Vol. 279, Issue 40 https://doi.org/10.1074/jbc.m405259200	journal	July 2004
The Regulatory Domain of SRK2E/OST1/SnRK2.6 Interacts with ABI1 and Integrates Abscisic Acid (ABA) and Osmotic Stress Signals Controlling Stomatal Closure in Arabidopsis Yoshida, Riichiro; Umezawa, Taishi; Mizoguchi, Tsuyoshi Journal of Biological Chemistry, Vol. 281, Issue 8 https://doi.org/10.1074/jbc.m509820200	journal	December 2005
The rapid generation of mutation data matrices from protein sequences Jones, David T.; Taylor, William R.; Thornton, Janet M. Bioinformatics, Vol. 8, Issue 3 https://doi.org/10.1093/bioinformatics/8.3.275	journal	January 1992
The Pfam protein families database: towards a more sustainable future Finn, Robert D.; Coggill, Penelope; Eberhardt, Ruth Y. Nucleic Acids Research, Vol. 44, Issue D1 https://doi.org/10.1093/nar/gkv1344	journal	December 2015
The EMBL-EBI search and sequence analysis tools APIs in 2019 Madeira, Fábio; Park, Young mi; Lee, Joon Nucleic Acids Research, Vol. 47, Issue W1 https://doi.org/10.1093/nar/gkz268	journal	April 2019
Three Arabidopsis SnRK2 Protein Kinases, SRK2D/SnRK2.2, SRK2E/SnRK2.6/OST1 and SRK2I/SnRK2.3, Involved in ABA Signaling are Essential for the Control of Seed Development and Dormancy Nakashima, Kazuo; Fujita, Yasunari; Kanamori, Norihito Plant and Cell Physiology, Vol. 50, Issue 7 https://doi.org/10.1093/pcp/pcp083	journal	June 2009
Three SnRK2 Protein Kinases are the Main Positive Regulators of Abscisic Acid Signaling in Response to Water Stress in Arabidopsis Fujita, Yasunari; Nakashima, Kazuo; Yoshida, Takuya Plant and Cell Physiology, Vol. 50, Issue 12 https://doi.org/10.1093/pcp/pcp147	journal	September 2009
Molecular Basis of the Core Regulatory Network in ABA Responses: Sensing, Signaling and Transport Umezawa, T.; Nakashima, K.; Miyakawa, T. Plant and Cell Physiology, Vol. 51, Issue 11 https://doi.org/10.1093/pcp/pcq156	journal	October 2010
Analysis and Functional Annotation of an Expressed Sequence Tag Collection for Tropical Crop Sugarcane Vettore, A. L. Genome Research, Vol. 13, Issue 12 https://doi.org/10.1101/gr.1532103	journal	December 2003
Arabidopsis OST1 Protein Kinase Mediates the Regulation of Stomatal Aperture by Abscisic Acid and Acts Upstream of Reactive Oxygen Species Production Mustilli, Anna-Chiara; Merlot, Sylvain; Vavasseur, Alain The Plant Cell, Vol. 14, Issue 12 https://doi.org/10.1105/tpc.007906	journal	November 2002
Differential Activation of the Rice Sucrose Nonfermenting1–Related Protein Kinase2 Family by Hyperosmotic Stress and Abscisic Acid Kobayashi, Yuhko; Yamamoto, Shuhei; Minami, Hideyuki The Plant Cell, Vol. 16, Issue 5 https://doi.org/10.1105/tpc.019943	journal	April 2004
Identification of Two Protein Kinases Required for Abscisic Acid Regulation of Seed Germination, Root Growth, and Gene Expression in Arabidopsis Fujii, Hiroaki; Verslues, Paul E.; Zhu, Jian-Kang The Plant Cell, Vol. 19, Issue 2 https://doi.org/10.1105/tpc.106.048538	journal	February 2007
Protein Phosphatases 2C Regulate the Activation of the Snf1-Related Kinase OST1 by Abscisic Acid in Arabidopsis Vlad, Florina; Rubio, Silvia; Rodrigues, Americo The Plant Cell, Vol. 21, Issue 10 https://doi.org/10.1105/tpc.109.069179	journal	October 2009
ZmOST1 mediates abscisic acid regulation of guard cell ion channels and drought stress responses Wu, Qiqi; Wang, Mei; Shen, Jianlin Journal of Integrative Plant Biology, Vol. 61, Issue 4 https://doi.org/10.1111/jipb.12714	journal	November 2018
Regulators of PP2C Phosphatase Activity Function as Abscisic Acid Sensors Ma, Y.; Szostkiewicz, I.; Korte, A. Science https://doi.org/10.1126/science.1172408	journal	April 2009
Abscisic Acid Inhibits Type 2C Protein Phosphatases via the PYR/PYL Family of START Proteins Park, S. -Y.; Fung, P.; Nishimura, N. Science https://doi.org/10.1126/science.1173041	journal	April 2009
Molecular Mimicry Regulates ABA Signaling by SnRK2 Kinases and PP2C Phosphatases Soon, Fen-Fen; Ng, Ley-Moy; Zhou, X. Edward Science, Vol. 335, Issue 6064 https://doi.org/10.1126/science.1215106	journal	November 2011
Genome-wide identification and characterization of SnRK2 gene family in cotton (Gossypium hirsutum L.) Liu, Zhao; Ge, Xiaoyang; Yang, Zuoren BMC Genetics, Vol. 18, Issue 1 https://doi.org/10.1186/s12863-017-0517-3	journal	June 2017
Overexpression of a Common Wheat Gene TaSnRK2.8 Enhances Tolerance to Drought, Salt and Low Temperature in Arabidopsis Zhang, Hongying; Mao, Xinguo; Wang, Chengshe PLoS ONE, Vol. 5, Issue 12 https://doi.org/10.1371/journal.pone.0016041	journal	December 2010
The Maize OST1 Kinase Homolog Phosphorylates and Regulates the Maize SNAC1-Type Transcription Factor Vilela, Belmiro; Moreno-Cortés, Alicia; Rabissi, Agnese PLoS ONE, Vol. 8, Issue 2 https://doi.org/10.1371/journal.pone.0058105	journal	February 2013

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59 BASIC BIOLOGICAL SCIENCES
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Title: The C-Terminal Domains SnRK2 Box and ABA Box Have a Role in Sugarcane SnRK2s Auto-Activation and Activity

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References (87)

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