A missense variant remote from the active site impairs stability of human phosphoglucomutase 1
Abstract
Abstract Missense variants of human phosphoglucomutase 1 (PGM1) cause the inherited metabolic disease known as PGM1 deficiency. This condition is categorised as both a glycogen storage disease and a congenital disorder of glycosylation. Approximately 20 missense variants of PGM1 are linked to PGM1 deficiency, and biochemical studies have suggested that they fall into two general categories: those affecting the active site and catalytic efficiency, and those that appear to impair protein folding and/or stability. In this study, we characterise a novel variant of Arg422, a residue distal from the active site of PGM1 and the site of a previously identified disease‐related variant (Arg422Trp). In prior studies, the R422W variant was found to produce insoluble protein in a recombinant expression system, precluding further in vitro characterisation. Here we investigate an alternative variant of this residue, Arg422Gln, which is amenable to experimental characterisation presumably due to its more conservative physicochemical substitution. Biochemical, crystallographic, and computational studies of R422Q establish that this variant causes only minor changes in catalytic efficiency and 3D structure, but is nonetheless dramatically reduced in stability. Unexpectedly, binding of a substrate analog is found to further destabilise the protein, in contrast to its stabilising effect on wild‐type PGM1 andmore »
- Authors:
-
- Department of Biochemistry University of Missouri Columbia Missouri USA
- College of Pharmacy Dalhousie University Halifax Nova Scotia Canada
- College of Pharmacy Dalhousie University Halifax Nova Scotia Canada, Department of Chemistry Dalhousie University Halifax Nova Scotia Canada
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1600567
- Grant/Contract Number:
- DE‐AC02‐05CH11231
- Resource Type:
- Publisher's Accepted Manuscript
- Journal Name:
- Journal of Inherited Metabolic Disease
- Additional Journal Information:
- Journal Name: Journal of Inherited Metabolic Disease Journal Volume: 43 Journal Issue: 4; Journal ID: ISSN 0141-8955
- Publisher:
- Wiley Blackwell (John Wiley & Sons)
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
Citation Formats
Stiers, Kyle M., Hansen, Reed P., Daghlas, Bana A., Mason, Kelly N., Zhu, Jian‐She, Jakeman, David L., and Beamer, Lesa J. A missense variant remote from the active site impairs stability of human phosphoglucomutase 1. Country unknown/Code not available: N. p., 2020.
Web. doi:10.1002/jimd.12222.
Stiers, Kyle M., Hansen, Reed P., Daghlas, Bana A., Mason, Kelly N., Zhu, Jian‐She, Jakeman, David L., & Beamer, Lesa J. A missense variant remote from the active site impairs stability of human phosphoglucomutase 1. Country unknown/Code not available. https://doi.org/10.1002/jimd.12222
Stiers, Kyle M., Hansen, Reed P., Daghlas, Bana A., Mason, Kelly N., Zhu, Jian‐She, Jakeman, David L., and Beamer, Lesa J. Tue .
"A missense variant remote from the active site impairs stability of human phosphoglucomutase 1". Country unknown/Code not available. https://doi.org/10.1002/jimd.12222.
@article{osti_1600567,
title = {A missense variant remote from the active site impairs stability of human phosphoglucomutase 1},
author = {Stiers, Kyle M. and Hansen, Reed P. and Daghlas, Bana A. and Mason, Kelly N. and Zhu, Jian‐She and Jakeman, David L. and Beamer, Lesa J.},
abstractNote = {Abstract Missense variants of human phosphoglucomutase 1 (PGM1) cause the inherited metabolic disease known as PGM1 deficiency. This condition is categorised as both a glycogen storage disease and a congenital disorder of glycosylation. Approximately 20 missense variants of PGM1 are linked to PGM1 deficiency, and biochemical studies have suggested that they fall into two general categories: those affecting the active site and catalytic efficiency, and those that appear to impair protein folding and/or stability. In this study, we characterise a novel variant of Arg422, a residue distal from the active site of PGM1 and the site of a previously identified disease‐related variant (Arg422Trp). In prior studies, the R422W variant was found to produce insoluble protein in a recombinant expression system, precluding further in vitro characterisation. Here we investigate an alternative variant of this residue, Arg422Gln, which is amenable to experimental characterisation presumably due to its more conservative physicochemical substitution. Biochemical, crystallographic, and computational studies of R422Q establish that this variant causes only minor changes in catalytic efficiency and 3D structure, but is nonetheless dramatically reduced in stability. Unexpectedly, binding of a substrate analog is found to further destabilise the protein, in contrast to its stabilising effect on wild‐type PGM1 and several other missense variants. This work establishes Arg422 as a lynchpin residue for the stability of PGM1 and supports the impairment of protein stability as a pathomechanism for variants that cause PGM1 deficiency. Synopsis Biochemical and structural studies of a missense variant far from the active site of human PGM1 identify a residue with a key role in enzyme stability.},
doi = {10.1002/jimd.12222},
journal = {Journal of Inherited Metabolic Disease},
number = 4,
volume = 43,
place = {Country unknown/Code not available},
year = {Tue Feb 18 00:00:00 EST 2020},
month = {Tue Feb 18 00:00:00 EST 2020}
}
https://doi.org/10.1002/jimd.12222
Web of Science
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