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Title: Cytosolic Acetyl-CoA Generated by ATP-Citrate Lyase Is Essential for Acetylation of Cell Wall Polysaccharides

Abstract

Abstract Plant cell wall polysaccharides, including xylan, glucomannan, xyloglucan and pectin, are often acetylated. Although a number of acetyltransferases responsible for the acetylation of some of these polysaccharides have been biochemically characterized, little is known about the source of acetyl donors and how acetyl donors are translocated into the Golgi, where these polysaccharides are synthesized. In this report, we investigated roles of ATP-citrate lyase (ACL) that generates cytosolic acetyl-CoA in cell wall polysaccharide acetylation and effects of simultaneous mutations of four Reduced Wall Acetylation (RWA) genes on acetyl-CoA transport into the Golgi in Arabidopsis thaliana. Expression analyses of genes involved in the generation of acetyl-CoA in different subcellular compartments showed that the expression of several ACL genes responsible for cytosolic acetyl-CoA synthesis was elevated in interfascicular fiber cells and induced by secondary wall-associated transcriptional activators. Simultaneous downregulation of the expression of ACL genes was demonstrated to result in a substantial decrease in the degree of xylan acetylation and a severe alteration in secondary wall structure in xylem vessels. In addition, the degree of acetylation of other cell wall polysaccharides, including glucomannan, xyloglucan and pectin, was also reduced. Moreover, Golgi-enriched membrane vesicles isolated from the rwa1/2/3/4 quadruple mutant were found tomore » exhibit a drastic reduction in acetyl-CoA transport activity compared with the wild type. These findings indicate that cytosolic acetyl-CoA generated by ACL is essential for cell wall polysaccharide acetylation and RWAs are required for its transport from the cytosol into the Golgi.« less

Authors:
 [1];  [2];  [3];  [2];  [4];  [4]; ORCiD logo [1]
  1. Department of Plant Biology, University of Georgia, Athens, GA 30602, USA
  2. Department of Chemistry, University of Georgia, Athens, GA 30602, USA
  3. Georgia Electron Microscopy, University of Georgia, Athens, GA 30602, USA
  4. Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602, USA
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1599930
Grant/Contract Number:  
SC0015662
Resource Type:
Published Article
Journal Name:
Plant and Cell Physiology
Additional Journal Information:
Journal Name: Plant and Cell Physiology Journal Volume: 61 Journal Issue: 1; Journal ID: ISSN 0032-0781
Publisher:
Oxford University Press
Country of Publication:
Japan
Language:
English

Citation Formats

Zhong, Ruiqin, Cui, Dongtao, Richardson, Elizabeth A., Phillips, Dennis R., Azadi, Parastoo, Lu, Grace, and Ye, Zheng-Hua. Cytosolic Acetyl-CoA Generated by ATP-Citrate Lyase Is Essential for Acetylation of Cell Wall Polysaccharides. Japan: N. p., 2019. Web. https://doi.org/10.1093/pcp/pcz178.
Zhong, Ruiqin, Cui, Dongtao, Richardson, Elizabeth A., Phillips, Dennis R., Azadi, Parastoo, Lu, Grace, & Ye, Zheng-Hua. Cytosolic Acetyl-CoA Generated by ATP-Citrate Lyase Is Essential for Acetylation of Cell Wall Polysaccharides. Japan. https://doi.org/10.1093/pcp/pcz178
Zhong, Ruiqin, Cui, Dongtao, Richardson, Elizabeth A., Phillips, Dennis R., Azadi, Parastoo, Lu, Grace, and Ye, Zheng-Hua. Tue . "Cytosolic Acetyl-CoA Generated by ATP-Citrate Lyase Is Essential for Acetylation of Cell Wall Polysaccharides". Japan. https://doi.org/10.1093/pcp/pcz178.
@article{osti_1599930,
title = {Cytosolic Acetyl-CoA Generated by ATP-Citrate Lyase Is Essential for Acetylation of Cell Wall Polysaccharides},
author = {Zhong, Ruiqin and Cui, Dongtao and Richardson, Elizabeth A. and Phillips, Dennis R. and Azadi, Parastoo and Lu, Grace and Ye, Zheng-Hua},
abstractNote = {Abstract Plant cell wall polysaccharides, including xylan, glucomannan, xyloglucan and pectin, are often acetylated. Although a number of acetyltransferases responsible for the acetylation of some of these polysaccharides have been biochemically characterized, little is known about the source of acetyl donors and how acetyl donors are translocated into the Golgi, where these polysaccharides are synthesized. In this report, we investigated roles of ATP-citrate lyase (ACL) that generates cytosolic acetyl-CoA in cell wall polysaccharide acetylation and effects of simultaneous mutations of four Reduced Wall Acetylation (RWA) genes on acetyl-CoA transport into the Golgi in Arabidopsis thaliana. Expression analyses of genes involved in the generation of acetyl-CoA in different subcellular compartments showed that the expression of several ACL genes responsible for cytosolic acetyl-CoA synthesis was elevated in interfascicular fiber cells and induced by secondary wall-associated transcriptional activators. Simultaneous downregulation of the expression of ACL genes was demonstrated to result in a substantial decrease in the degree of xylan acetylation and a severe alteration in secondary wall structure in xylem vessels. In addition, the degree of acetylation of other cell wall polysaccharides, including glucomannan, xyloglucan and pectin, was also reduced. Moreover, Golgi-enriched membrane vesicles isolated from the rwa1/2/3/4 quadruple mutant were found to exhibit a drastic reduction in acetyl-CoA transport activity compared with the wild type. These findings indicate that cytosolic acetyl-CoA generated by ACL is essential for cell wall polysaccharide acetylation and RWAs are required for its transport from the cytosol into the Golgi.},
doi = {10.1093/pcp/pcz178},
journal = {Plant and Cell Physiology},
number = 1,
volume = 61,
place = {Japan},
year = {2019},
month = {9}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1093/pcp/pcz178

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Cited by: 2 works
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