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Title: Biotransformation of 2,4‐dinitrotoluene in a phototrophic co‐culture of engineered Synechococcus elongatus and Pseudomonas putida

 [1];  [2];  [3]; ORCiD logo [3]; ORCiD logo [4]
  1. DOE‐MSU Plant Research Laboratories Michigan State University East Lansing MI USA, Genetics Program Michigan State University East Lansing MI USA
  2. Heinrich‐Heine Universität Düsseldorf Germany
  3. The Novo Nordisk Foundation Center for Biosustainability Technical University of Denmark Kgs Lyngby Denmark
  4. DOE‐MSU Plant Research Laboratories Michigan State University East Lansing MI USA, Genetics Program Michigan State University East Lansing MI USA, Department of Biochemistry and Molecular Biology Michigan State University East Lansing MI USA
Publication Date:
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OSTI Identifier:
Alternate Identifier(s):
OSTI ID: 1599924
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Resource Type:
Published Article
Journal Name:
Microbial Biotechnology (Online)
Additional Journal Information:
[Journal Name: Microbial Biotechnology (Online)]; Journal ID: ISSN 1751-7915
Country of Publication:
United Kingdom

Citation Formats

Fedeson, Derek T., Saake, Pia, Calero, Patricia, Nikel, Pablo Iván, and Ducat, Daniel C. Biotransformation of 2,4‐dinitrotoluene in a phototrophic co‐culture of engineered Synechococcus elongatus and Pseudomonas putida. United Kingdom: N. p., 2020. Web. doi:10.1111/1751-7915.13544.
Fedeson, Derek T., Saake, Pia, Calero, Patricia, Nikel, Pablo Iván, & Ducat, Daniel C. Biotransformation of 2,4‐dinitrotoluene in a phototrophic co‐culture of engineered Synechococcus elongatus and Pseudomonas putida. United Kingdom. doi:10.1111/1751-7915.13544.
Fedeson, Derek T., Saake, Pia, Calero, Patricia, Nikel, Pablo Iván, and Ducat, Daniel C. Sun . "Biotransformation of 2,4‐dinitrotoluene in a phototrophic co‐culture of engineered Synechococcus elongatus and Pseudomonas putida". United Kingdom. doi:10.1111/1751-7915.13544.
title = {Biotransformation of 2,4‐dinitrotoluene in a phototrophic co‐culture of engineered Synechococcus elongatus and Pseudomonas putida},
author = {Fedeson, Derek T. and Saake, Pia and Calero, Patricia and Nikel, Pablo Iván and Ducat, Daniel C.},
abstractNote = {},
doi = {10.1111/1751-7915.13544},
journal = {Microbial Biotechnology (Online)},
number = ,
volume = ,
place = {United Kingdom},
year = {2020},
month = {2}

Journal Article:
Free Publicly Available Full Text
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DOI: 10.1111/1751-7915.13544

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Works referenced in this record:

The Metabolic Redox Regime of Pseudomonas putida Tunes Its Evolvability toward Novel Xenobiotic Substrates
journal, August 2018

  • Akkaya, Özlem; Pérez-Pantoja, Danilo R.; Calles, Belén
  • mBio, Vol. 9, Issue 4
  • DOI: 10.1128/mBio.01512-18

Biodegradation of Nitroaromatic Compounds
journal, October 1995

Getting Bacteria in Shape: Synthetic Morphology Approaches for the Design of Efficient Microbial Cell Factories
journal, July 2018

Chemical characterization and toxicological testing of windrow composts from explosives-contaminated sediments
journal, January 1995

  • Griest, W. H.; Tyndall, R. L.; Stewart, A. J.
  • Environmental Toxicology and Chemistry, Vol. 14, Issue 1
  • DOI: 10.1002/etc.5620140107

Immobilization of Nicotiana tabacum plant cell suspensions within calcium alginate gel beads for the production of enhanced amounts of scopolin
journal, February 2000

The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes
journal, November 2012

  • Silva-Rocha, Rafael; Martínez-García, Esteban; Calles, Belén
  • Nucleic Acids Research, Vol. 41, Issue D1
  • DOI: 10.1093/nar/gks1119

Engineering Pseudomonas fluorescens for Biodegradation of 2,4-Dinitrotoluene
journal, December 2005

Evolutionary Approaches for Engineering Industrially Relevant Phenotypes in Bacterial Cell Factories
journal, June 2019

  • Fernández‐Cabezón, Lorena; Cros, Antonin; Nikel, Pablo I.
  • Biotechnology Journal, Vol. 14, Issue 9
  • DOI: 10.1002/biot.201800439

Nitroaromatic Compounds, from Synthesis to Biodegradation
journal, May 2010

  • Ju, K. -S.; Parales, R. E.
  • Microbiology and Molecular Biology Reviews, Vol. 74, Issue 2
  • DOI: 10.1128/MMBR.00006-10

Engineering sucrose metabolism in Pseudomonas putida highlights the importance of porins
journal, May 2018

  • Löwe, Hannes; Sinner, Peter; Kremling, Andreas
  • Microbial Biotechnology, Vol. 13, Issue 1
  • DOI: 10.1111/1751-7915.13283

Biotransformations of Explosives
journal, July 2001

  • French, Chris E.; Rosser, Susan J.; Bruce, Neil C.
  • Biotechnology and Genetic Engineering Reviews, Vol. 18, Issue 1
  • DOI: 10.1080/02648725.2001.10648013

Inhibition of Cyanobacterial Growth on a Municipal Wastewater Sidestream Is Impacted by Temperature
journal, February 2018

Metabolic engineering to expand the substrate spectrum of Pseudomonas putida toward sucrose
journal, March 2017

  • Löwe, Hannes; Schmauder, Lukas; Hobmeier, Karina
  • MicrobiologyOpen, Vol. 6, Issue 4
  • DOI: 10.1002/mbo3.473

A Post-translational Metabolic Switch Enables Complete Decoupling of Bacterial Growth from Biopolymer Production in Engineered Escherichia coli
journal, October 2018

  • Durante-Rodríguez, Gonzalo; de Lorenzo, Víctor; Nikel, Pablo I.
  • ACS Synthetic Biology, Vol. 7, Issue 11
  • DOI: 10.1021/acssynbio.8b00345

A synthetic, light-driven consortium of cyanobacteria and heterotrophic bacteria enables stable polyhydroxybutyrate production
journal, November 2017

Engineering microbial consortia by division of labor
journal, February 2019

Emerging strategies for engineering microbial communities
journal, November 2019

The potential of Synechococcus elongatus UTEX 2973 for sugar feedstock production
journal, April 2016

  • Song, Kuo; Tan, Xiaoming; Liang, Yajing
  • Applied Microbiology and Biotechnology, Vol. 100, Issue 18
  • DOI: 10.1007/s00253-016-7510-z

Synthetic microbial ecology and the dynamic interplay between microbial genotypes
journal, August 2016

  • Dolinšek, Jan; Goldschmidt, Felix; Johnson, David R.
  • FEMS Microbiology Reviews, Vol. 40, Issue 6
  • DOI: 10.1093/femsre/fuw024

Co-culture systems and technologies: taking synthetic biology to the next level
journal, July 2014

  • Goers, Lisa; Freemont, Paul; Polizzi, Karen M.
  • Journal of The Royal Society Interface, Vol. 11, Issue 96
  • DOI: 10.1098/rsif.2014.0065

Cloning and characterization of Pseudomonas sp. strain DNT genes for 2,4-dinitrotoluene degradation.
journal, January 1993

Synthetic photosynthetic consortia define interactions leading to robustness and photoproduction
journal, January 2017

  • Hays, Stephanie G.; Yan, Leo L. W.; Silver, Pamela A.
  • Journal of Biological Engineering, Vol. 11, Issue 1
  • DOI: 10.1186/s13036-017-0048-5

Toxicity of 2,4-dinitrotoluene to terrestrial plants in natural soils
journal, July 2010

Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non-traditional microorganisms
journal, June 2018

Genetic engineering of multispecies microbial cell factories as an alternative for bioenergy production
journal, September 2013

  • Ortiz-Marquez, Juan Cesar Federico; Do Nascimento, Mauro; Zehr, Jonathan Philip
  • Trends in Biotechnology, Vol. 31, Issue 9
  • DOI: 10.1016/j.tibtech.2013.05.006

Evolving metabolism of 2,4-dinitrotoluene triggers SOS-independent diversification of host cells: Stress-induced mutagenesis by DNT
journal, December 2018

  • Akkaya, Özlem; Nikel, Pablo I.; Pérez-Pantoja, Danilo
  • Environmental Microbiology, Vol. 21, Issue 1
  • DOI: 10.1111/1462-2920.14459

Stability and Diversity of Ecosystems
journal, July 2007

Extended H2 photoproduction by N2-fixing cyanobacteria immobilized in thin alginate films
journal, January 2012

Rerouting Carbon Flux To Enhance Photosynthetic Productivity
journal, February 2012

  • Ducat, Daniel C.; Avelar-Rivas, J. Abraham; Way, Jeffrey C.
  • Applied and Environmental Microbiology, Vol. 78, Issue 8
  • DOI: 10.1128/AEM.07901-11

2,4-Dinitrotoluene dioxygenase from Burkholderia sp. strain DNT: similarity to naphthalene dioxygenase.
journal, January 1996

Unraveling interactions in microbial communities - from co-cultures to microbiomes
journal, May 2015

Remediation of dinitrotoluene contaminated soils from former ammunition plants: soil washing efficiency and effective process monitoring in bioslurry reactors
journal, October 2001

  • Zhang, Chunlong; Daprato, Rebecca C.; Nishino, Shirley F.
  • Journal of Hazardous Materials, Vol. 87, Issue 1-3
  • DOI: 10.1016/S0304-3894(01)00240-0

Model microbial communities for ecosystems biology
journal, December 2017

Identification of biotransformation products from 2,4-dinitrotoluene.
journal, January 1978

The diversity–stability debate
journal, May 2000

From dirt to industrial applications: Pseudomonas putida as a Synthetic Biology chassis for hosting harsh biochemical reactions
journal, October 2016

A Designed A. vinelandiiS. elongatus Coculture for Chemical Photoproduction from Air, Water, Phosphate, and Trace Metals
journal, June 2016

Biodegradation of 4-methyl-5-nitrocatechol by Pseudomonas sp. strain DNT.
journal, January 1994

Nitrogen Starvation-Induced Chlorosis in Synechococcus PCC 7942. Low-Level Photosynthesis As a Mechanism of Long-Term Survival
journal, May 2001

  • Sauer, Jörg; Schreiber, Ulrich; Schmid, Roland
  • Plant Physiology, Vol. 126, Issue 1
  • DOI: 10.1104/pp.126.1.233

The best models of metabolism: The best models of metabolism
journal, May 2017

  • Voit, Eberhard O.
  • Wiley Interdisciplinary Reviews: Systems Biology and Medicine, Vol. 9, Issue 6
  • DOI: 10.1002/wsbm.1391

Growth of Chlamydomonas reinhardtii in acetate-free medium when co-cultured with alginate-encapsulated, acetate-producing strains of Synechococcus sp. PCC 7002
journal, October 2014

  • Therien, Jesse B.; Zadvornyy, Oleg A.; Posewitz, Matthew C.
  • Biotechnology for Biofuels, Vol. 7, Issue 1
  • DOI: 10.1186/s13068-014-0154-2

Irreversible binding of biologically reduced 2,4,6-trinitrotoluene to soil
journal, November 1999

  • Achtnich, Christof; Sieglen, Ute; Knackmuss, Hans-Joachim
  • Environmental Toxicology and Chemistry, Vol. 18, Issue 11
  • DOI: 10.1002/etc.5620181106

Bacterial pathways for degradation of nitroaromatics
journal, January 2006

  • Symons, Zoe C.; Bruce, Neil C.
  • Natural Product Reports, Vol. 23, Issue 6
  • DOI: 10.1039/b502796a

The private life of environmental bacteria: pollutant biodegradation at the single cell level: Phenotypic diversification of environmental bacteria
journal, January 2014

  • Nikel, Pablo Iván; Silva-Rocha, Rafael; Benedetti, Ilaria
  • Environmental Microbiology, Vol. 16, Issue 3
  • DOI: 10.1111/1462-2920.12360

Mutagenicity of Nitroaromatic Degradation Compounds
journal, January 2003

  • Padda, Ranjit S.; Wang, Chuanyue; Hughes, Joseph B.
  • Environmental Toxicology and Chemistry, Vol. 22, Issue 10
  • DOI: 10.1897/02-220

Aerobic Transformation of 2,4-Dinitrotoluene by Escherichia coli and Its Implications for the Detection of Trace Explosives
journal, December 2017

  • Shemer, Benjamin; Yagur-Kroll, Sharon; Hazan, Carina
  • Applied and Environmental Microbiology, Vol. 84, Issue 4
  • DOI: 10.1128/AEM.01729-17

Cloning and sequencing of the genes encoding 2-nitrotoluene dioxygenase from Pseudomonas sp. JS42
journal, November 1996

A holistic view of polyhydroxyalkanoate metabolism in Pseudomonas putida : Polyhydroxyalkanoate metabolism in
journal, February 2015

  • Prieto, Auxiliadora; Escapa, Isabel F.; Martínez, Virginia
  • Environmental Microbiology, Vol. 18, Issue 2
  • DOI: 10.1111/1462-2920.12760

Rules of Engagement: Interspecies Interactions that Regulate Microbial Communities
journal, October 2008

Endogenous Stress Caused by Faulty Oxidation Reactions Fosters Evolution of 2,4-Dinitrotoluene-Degrading Bacteria
journal, August 2013

Aerobic Degradation of Dinitrotoluenes and Pathway for Bacterial Degradation of 2,6-Dinitrotoluene
journal, May 2000

New Applications of Synthetic Biology Tools for Cyanobacterial Metabolic Engineering
journal, February 2019

  • Santos-Merino, María; Singh, Amit K.; Ducat, Daniel C.
  • Frontiers in Bioengineering and Biotechnology, Vol. 7
  • DOI: 10.3389/fbioe.2019.00033

Growth and hydrocarbon production ofBotryococcus braunii immobilized in calcium alginate gel
journal, December 1985

  • Bailliez, Christine; Largeau, Claude; Casadevall, Eliette
  • Applied Microbiology and Biotechnology, Vol. 23, Issue 2
  • DOI: 10.1007/BF01982724

Regulation of superoxide stress in Pseudomonas putida KT2440 is different from the SoxR paradigm in Escherichia coli
journal, March 2006

  • Park, Woojun; Peña-Llopis, Samuel; Lee, Yunho
  • Biochemical and Biophysical Research Communications, Vol. 341, Issue 1
  • DOI: 10.1016/j.bbrc.2005.12.142

Properties of the trihydroxytoluene oxygenase from Burkholderia cepacia R34: an extradiol dioxygenase from the 2,4-dinitrotoluene pathway
journal, December 1999

  • Johnson, Glenn R.; Jain, Rakesh K.; Spain, Jim C.
  • Archives of Microbiology, Vol. 173, Issue 2
  • DOI: 10.1007/s002039900111

Origins of the 2,4-Dinitrotoluene Pathway
journal, August 2002

Engineering cyanobacteria as photosynthetic feedstock factories
journal, February 2014

Interactions in a mixed bacterial population growing on methane in continuous culture
journal, January 1974

  • Wilkinson, T. G.; Topiwala, H. H.; Hamer, G.
  • Biotechnology and Bioengineering, Vol. 16, Issue 1
  • DOI: 10.1002/bit.260160105

Using artificial systems to explore the ecology and evolution of symbioses
journal, March 2011

  • Momeni, Babak; Chen, Chi-Chun; Hillesland, Kristina L.
  • Cellular and Molecular Life Sciences, Vol. 68, Issue 8
  • DOI: 10.1007/s00018-011-0649-y

Freeing P seudomonas putida  KT2440 of its proviral load strengthens endurance to environmental stresses: The prophages of P. putida  KT2440
journal, June 2014

  • Martínez-García, Esteban; Jatsenko, Tatjana; Kivisaar, Maia
  • Environmental Microbiology, Vol. 17, Issue 1
  • DOI: 10.1111/1462-2920.12492

Improving metabolite production in microbial co-cultures using a spatially constrained hydrogel: Improving Metabolite Production in Microbial Co-cultures
journal, January 2017

  • Smith, Matthew J.; Francis, Matthew B.
  • Biotechnology and Bioengineering, Vol. 114, Issue 6
  • DOI: 10.1002/bit.26235

Photoautotrophic production of polyhydroxyalkanoates in a synthetic mixed culture of Synechococcus elongatus cscB and Pseudomonas putida cscAB
journal, July 2017

Pseudomonas putida as a functional chassis for industrial biocatalysis: From native biochemistry to trans-metabolism
journal, November 2018

Emergent simplicity in microbial community assembly
journal, August 2018

Big questions, small worlds: microbial model systems in ecology
journal, April 2004

  • Jessup, Christine M.; Kassen, Rees; Forde, Samantha E.
  • Trends in Ecology & Evolution, Vol. 19, Issue 4
  • DOI: 10.1016/j.tree.2004.01.008

Biodegradation of 2,4-dinitrotoluene by a Pseudomonas sp.
journal, January 1991

Accelerated genome engineering of Pseudomonas putida by I‐ Sce I―mediated recombination and CRISPR ‐Cas9 counterselection
journal, March 2019

  • Wirth, Nicolas T.; Kozaeva, Ekaterina; Nikel, Pablo I.
  • Microbial Biotechnology, Vol. 13, Issue 1
  • DOI: 10.1111/1751-7915.13396

Genetic and metabolic advances in the engineering of cyanobacteria
journal, October 2019

Transgenic phytoremediation blasts onto the scene
journal, May 1999

  • Hooker, Brian S.; Skeen, Rodney S.
  • Nature Biotechnology, Vol. 17, Issue 5
  • DOI: 10.1038/8596

Growth and phosphorus removal by Synechococcus elongatus co-immobilized in alginate beads with Azospirillum brasilense
journal, October 2015

  • Ruiz-Güereca, Duahmet Abdul; Sánchez-Saavedra, M. del Pilar
  • Journal of Applied Phycology, Vol. 28, Issue 3
  • DOI: 10.1007/s10811-015-0728-9

Phytotransformation of 2,4-Dinitrotoluene in Arabidopsis thaliana: Toxicity, Fate, and Gene Expression Studies in Vitro
journal, December 2006

  • Yoon, J. M.; Oliver, D. J.; Shanks, J. V.
  • Biotechnology Progress, Vol. 22, Issue 6
  • DOI: 10.1021/bp0601443

Synthetic Biology Tools to Engineer Microbial Communities for Biotechnology
journal, February 2019

Regulation of polyhydroxyalkanoate biosynthesis in Pseudomonas putida and Pseudomonas aeruginosa
journal, August 2004

Oxidative stress in cyanobacteria
journal, March 2009