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Title: Plasticity of Phymatotrichopsis omnivora infection strategies is dependent on host and nonhost plant responses

Abstract

Necrotrophic fungi constitute the largest group of plant fungal pathogens that cause heavy crop losses worldwide. Phymatotrichopsis omnivora is a broad host, soil–borne necrotrophic fungal pathogen that infects over 2,000 dicotyledonous plants. The molecular basis of such broad host range is unknown. We conducted cell biology and transcriptomic studies in Medicago truncatula (susceptible), Brachypodium distachyon (resistant/nonhost), and Arabidopsis thaliana (partially resistant) to understand P. omnivora virulence mechanisms. We performed defence gene analysis, gene enrichments, and correlational network studies during key infection stages. We identified that P. omnivora infects the susceptible plant as a traditional necrotroph. However, it infects the partially resistant plant as a hemi–biotroph triggering salicylic acid–mediated defence pathways in the plant. Further, the infection strategy in partially resistant plants is determined by the host responses during early infection stages. Mutant analyses in A. thaliana established the role of small peptides PEP1 and PEP2 in defence against P. omnivora . The resistant/nonhost B. distachyon triggered stress responses involving sugars and aromatic acids. Bdwat1 mutant analysis identified the role of cell walls in defence. This is the first report that describes the plasticity in infection strategies of P. omnivora providing insights into broad host range.

Authors:
 [1];  [2]; ORCiD logo [1];  [2]; ORCiD logo [1]
  1. Noble Research Institute LLC Ardmore Oklahoma
  2. Biosciences DivisionOak Ridge National Laboratory Oak Ridge Tennessee, Bredesen Center for Interdisciplinary StudiesUniversity of Tennessee Knoxville Knoxville Tennessee
Publication Date:
Research Org.:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1599909
Alternate Identifier(s):
OSTI ID: 1599911; OSTI ID: 1648904
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Published Article
Journal Name:
Plant, Cell and Environment
Additional Journal Information:
Journal Name: Plant, Cell and Environment Journal Volume: 43 Journal Issue: 4; Journal ID: ISSN 0140-7791
Publisher:
Wiley
Country of Publication:
United Kingdom
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Fungal plasticity; hemi-biotrophy; necrotrophy; nonhost resistance; Phymatotrichopsis root rot; plant–fungal interactions; RNA sequencing; systems biology

Citation Formats

Kankanala, Prasanna, Jones, Piet, Nandety, Raja Sekhar, Jacobson, Daniel A., and Mysore, Kirankumar S. Plasticity of Phymatotrichopsis omnivora infection strategies is dependent on host and nonhost plant responses. United Kingdom: N. p., 2020. Web. doi:10.1111/pce.13721.
Kankanala, Prasanna, Jones, Piet, Nandety, Raja Sekhar, Jacobson, Daniel A., & Mysore, Kirankumar S. Plasticity of Phymatotrichopsis omnivora infection strategies is dependent on host and nonhost plant responses. United Kingdom. https://doi.org/10.1111/pce.13721
Kankanala, Prasanna, Jones, Piet, Nandety, Raja Sekhar, Jacobson, Daniel A., and Mysore, Kirankumar S. Fri . "Plasticity of Phymatotrichopsis omnivora infection strategies is dependent on host and nonhost plant responses". United Kingdom. https://doi.org/10.1111/pce.13721.
@article{osti_1599909,
title = {Plasticity of Phymatotrichopsis omnivora infection strategies is dependent on host and nonhost plant responses},
author = {Kankanala, Prasanna and Jones, Piet and Nandety, Raja Sekhar and Jacobson, Daniel A. and Mysore, Kirankumar S.},
abstractNote = {Necrotrophic fungi constitute the largest group of plant fungal pathogens that cause heavy crop losses worldwide. Phymatotrichopsis omnivora is a broad host, soil–borne necrotrophic fungal pathogen that infects over 2,000 dicotyledonous plants. The molecular basis of such broad host range is unknown. We conducted cell biology and transcriptomic studies in Medicago truncatula (susceptible), Brachypodium distachyon (resistant/nonhost), and Arabidopsis thaliana (partially resistant) to understand P. omnivora virulence mechanisms. We performed defence gene analysis, gene enrichments, and correlational network studies during key infection stages. We identified that P. omnivora infects the susceptible plant as a traditional necrotroph. However, it infects the partially resistant plant as a hemi–biotroph triggering salicylic acid–mediated defence pathways in the plant. Further, the infection strategy in partially resistant plants is determined by the host responses during early infection stages. Mutant analyses in A. thaliana established the role of small peptides PEP1 and PEP2 in defence against P. omnivora . The resistant/nonhost B. distachyon triggered stress responses involving sugars and aromatic acids. Bdwat1 mutant analysis identified the role of cell walls in defence. This is the first report that describes the plasticity in infection strategies of P. omnivora providing insights into broad host range.},
doi = {10.1111/pce.13721},
journal = {Plant, Cell and Environment},
number = 4,
volume = 43,
place = {United Kingdom},
year = {Fri Feb 14 00:00:00 EST 2020},
month = {Fri Feb 14 00:00:00 EST 2020}
}

Journal Article:
Free Publicly Available Full Text
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https://doi.org/10.1111/pce.13721

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