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Title: Ligand-conjugated quantum dots for fast sub-diffraction protein tracking in acute brain slices

Abstract

Semiconductor quantum dots (QDs) have demonstrated utility in long-term single particle tracking of membrane proteins in live cells in culture. To extend the superior optical properties of QDs to more physiologically relevant cell platforms, such as acute brain slices, we examine the photophysics of compact ligand-conjugated CdSe/CdS QDs using both ensemble and single particle analysis in brain tissue media. We find that symmetric core passivation is critical for both photostability in oxygenated media and for prolonged single particle imaging in brain slices. We then demonstrate the utility of these QDs by imaging single dopamine transporters in acute brain slices, achieving 20 nm localization precision at 10 Hz frame rates. Finally, these findings detail design requirements needed for new QD probes in complex living environments, and open the door to physiologically relevant studies that capture the utility of QD probes in acute brain slices.

Authors:
ORCiD logo [1]; ORCiD logo [2];  [1]; ORCiD logo [1];  [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [3]; ORCiD logo [1]
  1. Vanderbilt Univ., Nashville, TN (United States)
  2. Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Molecular Foundry
  3. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22), Materials Sciences & Engineering Division (SC-22.2); National Institutes of Health (NIH); Vanderbilt University; USDOE
OSTI Identifier:
1599822
Alternate Identifier(s):
OSTI ID: 1576688
Grant/Contract Number:  
[AC02-05CH11231; NIH T32GM065086-14; R01NS096317; NIH 5R01MH108562; CA68485; DK20593; DK58404; DK59637; EY08126]
Resource Type:
Accepted Manuscript
Journal Name:
Biomaterials Science
Additional Journal Information:
[ Journal Volume: 8; Journal Issue: 3]; Journal ID: ISSN 2047-4830
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Thal, Lucas B., Mann, Victor R., Sprinzen, David, McBride, James R., Reid, Kemar R., Tomlinson, Ian D., McMahon, Douglas G., Cohen, Bruce E., and Rosenthal, Sandra J. Ligand-conjugated quantum dots for fast sub-diffraction protein tracking in acute brain slices. United States: N. p., 2019. Web. doi:10.1039/c9bm01629e.
Thal, Lucas B., Mann, Victor R., Sprinzen, David, McBride, James R., Reid, Kemar R., Tomlinson, Ian D., McMahon, Douglas G., Cohen, Bruce E., & Rosenthal, Sandra J. Ligand-conjugated quantum dots for fast sub-diffraction protein tracking in acute brain slices. United States. doi:10.1039/c9bm01629e.
Thal, Lucas B., Mann, Victor R., Sprinzen, David, McBride, James R., Reid, Kemar R., Tomlinson, Ian D., McMahon, Douglas G., Cohen, Bruce E., and Rosenthal, Sandra J. Mon . "Ligand-conjugated quantum dots for fast sub-diffraction protein tracking in acute brain slices". United States. doi:10.1039/c9bm01629e.
@article{osti_1599822,
title = {Ligand-conjugated quantum dots for fast sub-diffraction protein tracking in acute brain slices},
author = {Thal, Lucas B. and Mann, Victor R. and Sprinzen, David and McBride, James R. and Reid, Kemar R. and Tomlinson, Ian D. and McMahon, Douglas G. and Cohen, Bruce E. and Rosenthal, Sandra J.},
abstractNote = {Semiconductor quantum dots (QDs) have demonstrated utility in long-term single particle tracking of membrane proteins in live cells in culture. To extend the superior optical properties of QDs to more physiologically relevant cell platforms, such as acute brain slices, we examine the photophysics of compact ligand-conjugated CdSe/CdS QDs using both ensemble and single particle analysis in brain tissue media. We find that symmetric core passivation is critical for both photostability in oxygenated media and for prolonged single particle imaging in brain slices. We then demonstrate the utility of these QDs by imaging single dopamine transporters in acute brain slices, achieving 20 nm localization precision at 10 Hz frame rates. Finally, these findings detail design requirements needed for new QD probes in complex living environments, and open the door to physiologically relevant studies that capture the utility of QD probes in acute brain slices.},
doi = {10.1039/c9bm01629e},
journal = {Biomaterials Science},
number = [3],
volume = [8],
place = {United States},
year = {2019},
month = {12}
}

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