In Situ Loading and Delivery of Short Single- and Double-Stranded DNA by Supramolecular Organic Frameworks

Yang, Bo; Zhang, Xiao-Dan; Li, Jian; Tian, Jia; Wu, Yi-Peng; Yu, Fa-Xing; Wang, Ruibing; Wang, Hui; Zhang, Dan-Wei; Liu, Yi; Zhou, Liu; Li, Zhan-Ting

doi:10.31635/ccschem.019.20180011

Title: In Situ Loading and Delivery of Short Single- and Double-Stranded DNA by Supramolecular Organic Frameworks

Journal Article · 04 June 2019 · CCS Chemistry

DOI: https://doi.org/10.31635/ccschem.019.20180011 · OSTI ID:1599800

Yang, Bo ^[1]; Zhang, Xiao-Dan ^[1]; Li, Jian ^[1]; Tian, Jia ^[1]; Wu, Yi-Peng ^[1]; Yu, Fa-Xing ^[1]; Wang, Ruibing ^[2]; Wang, Hui ^[1]; Zhang, Dan-Wei ^[1]; Liu, Yi ^[3]; Zhou, Liu ^[1]; Li, Zhan-Ting ^[1]

Fudan Univ., Shanghai (China)
Univ. of Macau, Taipa, Macao (China)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Short DNA represents an important class of biomacromolecules that are widely applied in gene therapy, editing, and modulation. However, the development of simple and reliable methods for their intracellular delivery remains a challenge. Herein, we describe that seven water-soluble, homogeneous supramolecular organic frameworks (SOFs) with a well-defined pore size and high stability in water that can accomplish in situ inclusion of single-stranded (ss) and double-stranded (ds) DNA (21, 23, and 58 nt) and effective intracellular delivery (including two noncancerous and six cancerous cell lines). Fluorescence quenching experiments for single and double end-labeled ss- and ds-DNA support that the DNA sequences can be completely enveloped by the SOFs. Confocal laser scanning microscopy and flow cytometry reveal that five of the SOFs exhibit excellent delivery efficiencies that, in most of the studied cases, outperform the commercial standard Lipo2000, even at low SOF–nucleic acid ratios. In addition to high delivery efficiencies, the water-soluble, self-assembled SOF carriers have a variety of advantages, including convenient preparation, high stability, and in situ DNA inclusion, which are all critical for practical applications in nucleic acid delivery.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Natural Science Foundation of China (NSFC)

Grant/Contract Number:: AC02-05CH11231; 21432004; 21529201

OSTI ID:: 1599800

Journal Information:: CCS Chemistry, Vol. 1, Issue 2; ISSN 2096-5745

Publisher:: Chinese Chemical Society PublishingCopyright Statement

Country of Publication:: United States

Language:: English

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Title: In Situ Loading and Delivery of Short Single- and Double-Stranded DNA by Supramolecular Organic Frameworks

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