Aza-proline effectively mimics L-proline stereochemistry in triple helical collagen

Kasznel, Alexander J.; Harris, Trevor; Porter, Nicholas J.; Zhang, Yitao; Chenoweth, David M.

doi:10.1039/C9SC02211B

Title: Aza-proline effectively mimics L-proline stereochemistry in triple helical collagen

Journal Article · 21 June 2019 · Chemical Science

DOI: https://doi.org/10.1039/C9SC02211B · OSTI ID:1599449

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Univ. of Pennsylvania, Philadelphia, PA (United States)

The prevalence of L-amino acids in biomolecules has been shown to have teleological importance in biomolecular structure and self-assembly. Recently, biophysical studies have demonstrated that natural L-amino acids can be replaced with non-natural achiral aza-amino acids in folded protein structures such as triple helical collagen. However, the structural consequences of achiral aza-amino acid incorporation has not been elucidated in the context of any relevant folded biomolecule. Herein, we use X-ray crystallography to provide the first atomic resolution crystal structure of an achiral aza-amino acid residue embedded within a folded protein structure, definitively illustrating that achiral aza-proline has the capacity to effectively mimic the stereochemistry of natural amino acids within the context of triple helical collagen. We further corroborate this finding with density functional theory computational analysis showing that the natural L-amino acid stereochemistry for aza-proline is energetically favored when arranged in the aza-proline-hydroxyproline-glycine motif. In addition to providing fundamental insight into peptide and protein structure, the incorporation of achiral stereochemical mimics such as aza-amino acids could have far reaching impacts in areas ranging from synthetic materials to drug design.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: National Science Foundation (NSF); University of Pennsylvania; National Institutes of Health (NIH)

Grant/Contract Number:: DMR-1720530

OSTI ID:: 1599449

Journal Information:: Chemical Science, Vol. 10, Issue 29; ISSN 2041-6520

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 9 works

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