Exploiting nonionic surfactants to enhance fatty alcohol production in Rhodosporidium toruloides

Liu, Di; Geiselman, Gina M.; Coradetti, Samuel; Cheng, Ya‐Fang; Kirby, James; Prahl, Jan‐Philip; Jacobson, Oslo; Sundstrom, Eric R.; Tanjore, Deepti; Skerker, Jeffrey M.; Gladden, John

doi:10.1002/bit.27285

Title: Exploiting nonionic surfactants to enhance fatty alcohol production in Rhodosporidium toruloides

Journal Article · 25 January 2020 · Biotechnology and Bioengineering

DOI: https://doi.org/10.1002/bit.27285 · OSTI ID:1599231

Liu, Di ^[1]; Geiselman, Gina M. ^[1]; Coradetti, Samuel ^[1]; Cheng, Ya‐Fang ^[2]; Kirby, James ^[1]; Prahl, Jan‐Philip ^[3]; Jacobson, Oslo ^[3]; Sundstrom, Eric R. ^[3]; Tanjore, Deepti ^[3]; Skerker, Jeffrey M. ^[2];

^[4]

Department of Biomass Science and Conversion TechnologySandia National Laboratories Livermore California, Department of EnergyAgile BioFoundry Emeryville California
QB3‐BerkeleyUniversity of California Berkeley California
Department of EnergyAgile BioFoundry Emeryville California, Biological Systems and Engineering DivisionLawrence Berkeley National Laboratory Berkeley California, Advanced Biofuels and Bioproducts Process Development UnitLawrence Berkeley National Laboratory Emeryville California
Department of Biomass Science and Conversion TechnologySandia National Laboratories Livermore California, Department of EnergyAgile BioFoundry Emeryville California, Joint BioEnergy Institute Emeryville California

Fatty alcohols (FOHs) are important feedstocks in the chemical industry to produce detergents, cosmetics, and lubricants. Microbial production of FOHs has become an attractive alternative to production in plants and animals due to growing energy demands and environmental concerns. However, inhibition of cell growth caused by intracellular FOH accumulation is one major issue that limits FOH titers in microbial hosts. In addition, identification of FOH-specific exporters remains a challenge and previous studies towards this end are limited. To alleviate the toxicity issue, we exploited nonionic surfactants to promote the export of FOHs in Rhodosporidium toruloides, an oleaginous yeast that is considered an attractive next-generation host for the production of fatty acid-derived chemicals. Our results showed FOH export efficiency was dramatically improved and the growth inhibition was alleviated in the presence of small amounts of tergitol and other surfactants. As a result, FOH titers increase by 4.3-fold at bench scale to 352.6 mg/L. With further process optimization in a 2-L bioreactor, the titer was further increased to 1.6 g/L. The method we show here can potentially be applied to other microbial hosts and may facilitate the commercialization of microbial FOH production.

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Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Renewable Power Office. Wind Energy Technologies Office

Grant/Contract Number:: AC04-94AL85000; AC02-05CH11231; NA0003525; NA0003525; AC02-05CH11231

OSTI ID:: 1599231

Journal Information:: Biotechnology and Bioengineering, Journal Name: Biotechnology and Bioengineering; ISSN 0006-3592

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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