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Title: Exploiting nonionic surfactants to enhance fatty alcohol production in Rhodosporidium toruloides

Abstract

Fatty alcohols (FOHs) are important feedstocks in the chemical industry to produce detergents, cosmetics, and lubricants. Microbial production of FOHs has become an attractive alternative to production in plants and animals due to growing energy demands and environmental concerns. However, inhibition of cell growth caused by intracellular FOH accumulation is one major issue that limits FOH titers in microbial hosts. In addition, identification of FOH-specific exporters remains a challenge and previous studies towards this end are limited. To alleviate the toxicity issue, we exploited nonionic surfactants to promote the export of FOHs in Rhodosporidium toruloides, an oleaginous yeast that is considered an attractive next-generation host for the production of fatty acid-derived chemicals. Our results showed FOH export efficiency was dramatically improved and the growth inhibition was alleviated in the presence of small amounts of tergitol and other surfactants. As a result, FOH titers increase by 4.3-fold at bench scale to 352.6 mg/L. With further process optimization in a 2-L bioreactor, the titer was further increased to 1.6 g/L. The method we show here can potentially be applied to other microbial hosts and may facilitate the commercialization of microbial FOH production.

Authors:
 [1];  [1];  [1];  [2];  [1];  [3];  [3];  [3];  [3];  [2]; ORCiD logo [4]
  1. Department of Biomass Science and Conversion TechnologySandia National Laboratories Livermore California, Department of EnergyAgile BioFoundry Emeryville California
  2. QB3‐BerkeleyUniversity of California Berkeley California
  3. Department of EnergyAgile BioFoundry Emeryville California, Biological Systems and Engineering DivisionLawrence Berkeley National Laboratory Berkeley California, Advanced Biofuels and Bioproducts Process Development UnitLawrence Berkeley National Laboratory Emeryville California
  4. Department of Biomass Science and Conversion TechnologySandia National Laboratories Livermore California, Department of EnergyAgile BioFoundry Emeryville California, Joint BioEnergy Institute Emeryville California
Publication Date:
Research Org.:
Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Renewable Power Office. Wind Energy Technologies Office
OSTI Identifier:
1599231
Alternate Identifier(s):
OSTI ID: 1599233; OSTI ID: 1601256
Report Number(s):
SAND-2020-1694J
Journal ID: ISSN 0006-3592
Grant/Contract Number:  
AC04-94AL85000; AC02‐05CH11231; NA0003525
Resource Type:
Published Article
Journal Name:
Biotechnology and Bioengineering
Additional Journal Information:
Journal Name: Biotechnology and Bioengineering; Journal ID: ISSN 0006-3592
Publisher:
Wiley
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; fatty alcohol; nonionic surfactants; product export; R. toruloides

Citation Formats

Liu, Di, Geiselman, Gina M., Coradetti, Samuel, Cheng, Ya‐Fang, Kirby, James, Prahl, Jan‐Philip, Jacobson, Oslo, Sundstrom, Eric R., Tanjore, Deepti, Skerker, Jeffrey M., and Gladden, John. Exploiting nonionic surfactants to enhance fatty alcohol production in Rhodosporidium toruloides. United States: N. p., 2020. Web. doi:10.1002/bit.27285.
Liu, Di, Geiselman, Gina M., Coradetti, Samuel, Cheng, Ya‐Fang, Kirby, James, Prahl, Jan‐Philip, Jacobson, Oslo, Sundstrom, Eric R., Tanjore, Deepti, Skerker, Jeffrey M., & Gladden, John. Exploiting nonionic surfactants to enhance fatty alcohol production in Rhodosporidium toruloides. United States. doi:10.1002/bit.27285.
Liu, Di, Geiselman, Gina M., Coradetti, Samuel, Cheng, Ya‐Fang, Kirby, James, Prahl, Jan‐Philip, Jacobson, Oslo, Sundstrom, Eric R., Tanjore, Deepti, Skerker, Jeffrey M., and Gladden, John. Sat . "Exploiting nonionic surfactants to enhance fatty alcohol production in Rhodosporidium toruloides". United States. doi:10.1002/bit.27285.
@article{osti_1599231,
title = {Exploiting nonionic surfactants to enhance fatty alcohol production in Rhodosporidium toruloides},
author = {Liu, Di and Geiselman, Gina M. and Coradetti, Samuel and Cheng, Ya‐Fang and Kirby, James and Prahl, Jan‐Philip and Jacobson, Oslo and Sundstrom, Eric R. and Tanjore, Deepti and Skerker, Jeffrey M. and Gladden, John},
abstractNote = {Fatty alcohols (FOHs) are important feedstocks in the chemical industry to produce detergents, cosmetics, and lubricants. Microbial production of FOHs has become an attractive alternative to production in plants and animals due to growing energy demands and environmental concerns. However, inhibition of cell growth caused by intracellular FOH accumulation is one major issue that limits FOH titers in microbial hosts. In addition, identification of FOH-specific exporters remains a challenge and previous studies towards this end are limited. To alleviate the toxicity issue, we exploited nonionic surfactants to promote the export of FOHs in Rhodosporidium toruloides, an oleaginous yeast that is considered an attractive next-generation host for the production of fatty acid-derived chemicals. Our results showed FOH export efficiency was dramatically improved and the growth inhibition was alleviated in the presence of small amounts of tergitol and other surfactants. As a result, FOH titers increase by 4.3-fold at bench scale to 352.6 mg/L. With further process optimization in a 2-L bioreactor, the titer was further increased to 1.6 g/L. The method we show here can potentially be applied to other microbial hosts and may facilitate the commercialization of microbial FOH production.},
doi = {10.1002/bit.27285},
journal = {Biotechnology and Bioengineering},
number = ,
volume = ,
place = {United States},
year = {2020},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1002/bit.27285

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