Visualizing Microbial Community Dynamics via a Controllable Soil Environment
Abstract
Visualizing microbial community dynamics within soil environments remains challenging. Here, we developed a custom-built SoilBox system that facilitates probing various changes occurring in microbial community development and molecular processes when exposed to environmental perturbations (e.g., temperature changes, drought, nutrient availability). Using confocal microscopy along with mass spectrometry imaging, we demonstrate the power of this system by correlating microbial growth and spatial metabolic information from soil communities under different moisture regimes.
- Authors:
- Publication Date:
- Research Org.:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1598721
- Alternate Identifier(s):
- OSTI ID: 1600533
- Report Number(s):
- PNNL-SA-147223
Journal ID: ISSN 2379-5077; e00645-19
- Grant/Contract Number:
- AC05-76RL01830
- Resource Type:
- Published Article
- Journal Name:
- mSystems
- Additional Journal Information:
- Journal Name: mSystems Journal Volume: 5 Journal Issue: 1; Journal ID: ISSN 2379-5077
- Publisher:
- American Society for Microbiology
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Bhattacharjee, Arunima, Velickovic, Dusan, Wietsma, Thomas W., Bell, Sheryl L., Jansson, Janet K., Hofmockel, Kirsten S., Anderton, Christopher R., and Wilmes, ed., Paul. Visualizing Microbial Community Dynamics via a Controllable Soil Environment. United States: N. p., 2020.
Web. doi:10.1128/mSystems.00645-19.
Bhattacharjee, Arunima, Velickovic, Dusan, Wietsma, Thomas W., Bell, Sheryl L., Jansson, Janet K., Hofmockel, Kirsten S., Anderton, Christopher R., & Wilmes, ed., Paul. Visualizing Microbial Community Dynamics via a Controllable Soil Environment. United States. https://doi.org/10.1128/mSystems.00645-19
Bhattacharjee, Arunima, Velickovic, Dusan, Wietsma, Thomas W., Bell, Sheryl L., Jansson, Janet K., Hofmockel, Kirsten S., Anderton, Christopher R., and Wilmes, ed., Paul. Tue .
"Visualizing Microbial Community Dynamics via a Controllable Soil Environment". United States. https://doi.org/10.1128/mSystems.00645-19.
@article{osti_1598721,
title = {Visualizing Microbial Community Dynamics via a Controllable Soil Environment},
author = {Bhattacharjee, Arunima and Velickovic, Dusan and Wietsma, Thomas W. and Bell, Sheryl L. and Jansson, Janet K. and Hofmockel, Kirsten S. and Anderton, Christopher R. and Wilmes, ed., Paul},
abstractNote = {Visualizing microbial community dynamics within soil environments remains challenging. Here, we developed a custom-built SoilBox system that facilitates probing various changes occurring in microbial community development and molecular processes when exposed to environmental perturbations (e.g., temperature changes, drought, nutrient availability). Using confocal microscopy along with mass spectrometry imaging, we demonstrate the power of this system by correlating microbial growth and spatial metabolic information from soil communities under different moisture regimes.},
doi = {10.1128/mSystems.00645-19},
journal = {mSystems},
number = 1,
volume = 5,
place = {United States},
year = {2020},
month = {2}
}
Free Publicly Available Full Text
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https://doi.org/10.1128/mSystems.00645-19
https://doi.org/10.1128/mSystems.00645-19
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Cited by: 7 works
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