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Title: Visualizing Microbial Community Dynamics via a Controllable Soil Environment

Abstract

Visualizing microbial community dynamics within soil environments remains challenging. Here, we developed a custom-built SoilBox system that facilitates probing various changes occurring in microbial community development and molecular processes when exposed to environmental perturbations (e.g., temperature changes, drought, nutrient availability). Using confocal microscopy along with mass spectrometry imaging, we demonstrate the power of this system by correlating microbial growth and spatial metabolic information from soil communities under different moisture regimes.

Authors:
; ; ; ; ; ; ORCiD logo;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1598721
Alternate Identifier(s):
OSTI ID: 1600533
Report Number(s):
PNNL-SA-147223
Journal ID: ISSN 2379-5077; e00645-19
Grant/Contract Number:  
AC05-76RL01830
Resource Type:
Published Article
Journal Name:
mSystems
Additional Journal Information:
Journal Name: mSystems Journal Volume: 5 Journal Issue: 1; Journal ID: ISSN 2379-5077
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Bhattacharjee, Arunima, Velickovic, Dusan, Wietsma, Thomas W., Bell, Sheryl L., Jansson, Janet K., Hofmockel, Kirsten S., Anderton, Christopher R., and Wilmes, ed., Paul. Visualizing Microbial Community Dynamics via a Controllable Soil Environment. United States: N. p., 2020. Web. doi:10.1128/mSystems.00645-19.
Bhattacharjee, Arunima, Velickovic, Dusan, Wietsma, Thomas W., Bell, Sheryl L., Jansson, Janet K., Hofmockel, Kirsten S., Anderton, Christopher R., & Wilmes, ed., Paul. Visualizing Microbial Community Dynamics via a Controllable Soil Environment. United States. https://doi.org/10.1128/mSystems.00645-19
Bhattacharjee, Arunima, Velickovic, Dusan, Wietsma, Thomas W., Bell, Sheryl L., Jansson, Janet K., Hofmockel, Kirsten S., Anderton, Christopher R., and Wilmes, ed., Paul. Tue . "Visualizing Microbial Community Dynamics via a Controllable Soil Environment". United States. https://doi.org/10.1128/mSystems.00645-19.
@article{osti_1598721,
title = {Visualizing Microbial Community Dynamics via a Controllable Soil Environment},
author = {Bhattacharjee, Arunima and Velickovic, Dusan and Wietsma, Thomas W. and Bell, Sheryl L. and Jansson, Janet K. and Hofmockel, Kirsten S. and Anderton, Christopher R. and Wilmes, ed., Paul},
abstractNote = {Visualizing microbial community dynamics within soil environments remains challenging. Here, we developed a custom-built SoilBox system that facilitates probing various changes occurring in microbial community development and molecular processes when exposed to environmental perturbations (e.g., temperature changes, drought, nutrient availability). Using confocal microscopy along with mass spectrometry imaging, we demonstrate the power of this system by correlating microbial growth and spatial metabolic information from soil communities under different moisture regimes.},
doi = {10.1128/mSystems.00645-19},
journal = {mSystems},
number = 1,
volume = 5,
place = {United States},
year = {2020},
month = {2}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1128/mSystems.00645-19

Citation Metrics:
Cited by: 7 works
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