Enterococcus faecalis α1–2‐mannosidase (EfMan‐I): an efficient catalyst for glycoprotein N‐glycan modification

Li, Yanhong; Li, Riyao; Yu, Hai; Sheng, Xue; Wang, Jing; Fisher, Andrew J.; Chen, Xi

doi:10.1002/1873-3468.13618

Title: Enterococcus faecalis α1–2‐mannosidase (EfMan‐I): an efficient catalyst for glycoprotein N‐glycan modification

Journal Article · 07 October 2019 · FEBS Letters

DOI: https://doi.org/10.1002/1873-3468.13618 · OSTI ID:1598708

Li, Yanhong ^[1]; Li, Riyao ^[1]; Yu, Hai ^[1]; Sheng, Xue ^[1]; Wang, Jing ^[2]; Fisher, Andrew J. ^[3];

^[1]

Department of Chemistry University of California Davis CA USA
Department of Chemistry University of California Davis CA USA, Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences Qingdao China
Department of Chemistry University of California Davis CA USA, Department of Molecular and Cellular Biology University of California Davis CA USA

While multiple α 1–2‐mannosidases are necessary for glycoprotein N‐glycan maturation in vertebrates, a single bacterial α1–2‐mannosidase can be sufficient to cleave all α1–2‐linked mannose residues in host glycoprotein N‐glycans. We report here the characterization and crystal structure of a new α1–2‐mannosidase (EfMan‐I) from Enterococcus faecalis , a Gram‐positive opportunistic human pathogen. EfMan‐I catalyzes the cleavage of α1–2‐mannose from not only oligomannoses but also high‐mannose‐type N‐glycans on glycoproteins. Its 2.15 Å resolution crystal structure reveals a two‐domain enzyme fold similar to other CAZy GH92 mannosidases. An unexpected potassium ion was observed bridging two domains near the active site. These findings support EfMan‐I as an effective catalyst for in vitro N‐glycan modification of glycoproteins with high‐mannose‐type N‐glycans.

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Sponsoring Organization:: USDOE

OSTI ID:: 1598708

Journal Information:: FEBS Letters, Journal Name: FEBS Letters Journal Issue: 3 Vol. 594; ISSN 0014-5793

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: Netherlands

Language:: English

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