Enterococcus faecalis α1–2‐mannosidase (EfMan‐I): an efficient catalyst for glycoprotein N‐glycan modification
Abstract
While multiple α 1–2‐mannosidases are necessary for glycoprotein N‐glycan maturation in vertebrates, a single bacterial α1–2‐mannosidase can be sufficient to cleave all α1–2‐linked mannose residues in host glycoprotein N‐glycans. We report here the characterization and crystal structure of a new α1–2‐mannosidase (EfMan‐I) from Enterococcus faecalis , a Gram‐positive opportunistic human pathogen. EfMan‐I catalyzes the cleavage of α1–2‐mannose from not only oligomannoses but also high‐mannose‐type N‐glycans on glycoproteins. Its 2.15 Å resolution crystal structure reveals a two‐domain enzyme fold similar to other CAZy GH92 mannosidases. An unexpected potassium ion was observed bridging two domains near the active site. These findings support EfMan‐I as an effective catalyst for in vitro N‐glycan modification of glycoproteins with high‐mannose‐type N‐glycans.
- Authors:
-
[1]
- Department of Chemistry University of California Davis CA USA
- Department of Chemistry University of California Davis CA USA, Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences Qingdao China
- Department of Chemistry University of California Davis CA USA, Department of Molecular and Cellular Biology University of California Davis CA USA
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1598708
- Resource Type:
- Publisher's Accepted Manuscript
- Journal Name:
- FEBS Letters
- Additional Journal Information:
- Journal Name: FEBS Letters Journal Volume: 594 Journal Issue: 3; Journal ID: ISSN 0014-5793
- Publisher:
- Wiley Blackwell (John Wiley & Sons)
- Country of Publication:
- Netherlands
- Language:
- English
Citation Formats
Li, Yanhong, Li, Riyao, Yu, Hai, Sheng, Xue, Wang, Jing, Fisher, Andrew J., and Chen, Xi. Enterococcus faecalis α1–2‐mannosidase (EfMan‐I): an efficient catalyst for glycoprotein N‐glycan modification. Netherlands: N. p., 2019.
Web. doi:10.1002/1873-3468.13618.
Li, Yanhong, Li, Riyao, Yu, Hai, Sheng, Xue, Wang, Jing, Fisher, Andrew J., & Chen, Xi. Enterococcus faecalis α1–2‐mannosidase (EfMan‐I): an efficient catalyst for glycoprotein N‐glycan modification. Netherlands. https://doi.org/10.1002/1873-3468.13618
Li, Yanhong, Li, Riyao, Yu, Hai, Sheng, Xue, Wang, Jing, Fisher, Andrew J., and Chen, Xi. Tue .
"Enterococcus faecalis α1–2‐mannosidase (EfMan‐I): an efficient catalyst for glycoprotein N‐glycan modification". Netherlands. https://doi.org/10.1002/1873-3468.13618.
@article{osti_1598708,
title = {Enterococcus faecalis α1–2‐mannosidase (EfMan‐I): an efficient catalyst for glycoprotein N‐glycan modification},
author = {Li, Yanhong and Li, Riyao and Yu, Hai and Sheng, Xue and Wang, Jing and Fisher, Andrew J. and Chen, Xi},
abstractNote = {While multiple α 1–2‐mannosidases are necessary for glycoprotein N‐glycan maturation in vertebrates, a single bacterial α1–2‐mannosidase can be sufficient to cleave all α1–2‐linked mannose residues in host glycoprotein N‐glycans. We report here the characterization and crystal structure of a new α1–2‐mannosidase (EfMan‐I) from Enterococcus faecalis , a Gram‐positive opportunistic human pathogen. EfMan‐I catalyzes the cleavage of α1–2‐mannose from not only oligomannoses but also high‐mannose‐type N‐glycans on glycoproteins. Its 2.15 Å resolution crystal structure reveals a two‐domain enzyme fold similar to other CAZy GH92 mannosidases. An unexpected potassium ion was observed bridging two domains near the active site. These findings support EfMan‐I as an effective catalyst for in vitro N‐glycan modification of glycoproteins with high‐mannose‐type N‐glycans.},
doi = {10.1002/1873-3468.13618},
journal = {FEBS Letters},
number = 3,
volume = 594,
place = {Netherlands},
year = {Tue Oct 08 00:00:00 EDT 2019},
month = {Tue Oct 08 00:00:00 EDT 2019}
}
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