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Title: Bayesian algorithms for recovering structure from single-particle diffraction snapshots of unknown orientation: a comparison

Abstract

The advent of X-ray free-electron lasers promises the possibility to determine the structure of individual particles such as microcrystallites, viruses and biomolecules from single-shot diffraction snapshots obtained before the particle is destroyed by the intense femtosecond pulse. This program requires the ability to determine the orientation of the particle giving rise to each snapshot at signal levels as low as ~10-2 photons per pixel. Two apparently different approaches have recently demonstrated this capability. Here we show they represent different implementations of the same fundamental approach, and identify the primary factors limiting their performance.

Authors:
 [1];  [1]
  1. Univ. of Wisconsin, Milwaukee, WI (United States)
Publication Date:
Research Org.:
Univ. of Wisconsin, Milwaukee, WI (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1598203
Grant/Contract Number:  
SC0002164
Resource Type:
Accepted Manuscript
Journal Name:
Acta Crystallographica. Section A, Foundations of Crystallography
Additional Journal Information:
Journal Volume: 67; Journal Issue: 5; Journal ID: ISSN 0108-7673
Publisher:
International Union of Crystallography
Country of Publication:
United States
Language:
English
Subject:
72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS

Citation Formats

Moths, Brian, and Ourmazd, Abbas. Bayesian algorithms for recovering structure from single-particle diffraction snapshots of unknown orientation: a comparison. United States: N. p., 2011. Web. doi:10.1107/S0108767311019611.
Moths, Brian, & Ourmazd, Abbas. Bayesian algorithms for recovering structure from single-particle diffraction snapshots of unknown orientation: a comparison. United States. https://doi.org/10.1107/S0108767311019611
Moths, Brian, and Ourmazd, Abbas. Wed . "Bayesian algorithms for recovering structure from single-particle diffraction snapshots of unknown orientation: a comparison". United States. https://doi.org/10.1107/S0108767311019611. https://www.osti.gov/servlets/purl/1598203.
@article{osti_1598203,
title = {Bayesian algorithms for recovering structure from single-particle diffraction snapshots of unknown orientation: a comparison},
author = {Moths, Brian and Ourmazd, Abbas},
abstractNote = {The advent of X-ray free-electron lasers promises the possibility to determine the structure of individual particles such as microcrystallites, viruses and biomolecules from single-shot diffraction snapshots obtained before the particle is destroyed by the intense femtosecond pulse. This program requires the ability to determine the orientation of the particle giving rise to each snapshot at signal levels as low as ~10-2 photons per pixel. Two apparently different approaches have recently demonstrated this capability. Here we show they represent different implementations of the same fundamental approach, and identify the primary factors limiting their performance.},
doi = {10.1107/S0108767311019611},
journal = {Acta Crystallographica. Section A, Foundations of Crystallography},
number = 5,
volume = 67,
place = {United States},
year = {Wed Jul 06 00:00:00 EDT 2011},
month = {Wed Jul 06 00:00:00 EDT 2011}
}

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