Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803

doi:10.1186/s13068-020-1653-y

Title: Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803

Abstract

Cyanobacteria can be metabolically engineered to convert CO₂ to fuels and chemicals such as ethylene. A major challenge in such efforts is to optimize carbon fixation and partition towards target molecules. The efe gene encoding an ethylene-forming enzyme was introduced into a strain of the cyanobacterium Synechocystis PCC 6803 with increased phosphoenolpyruvate carboxylase (PEPc) levels. The resulting engineered strain (CD-P) showed significantly increased ethylene production (10.5 ± 3.1 ug mL^-1 OD^-1 day^-1) compared to the control strain (6.4 ± 1.4 ug mL^-1 OD^-1 day^-1). Interestingly, extra copies of the native pepc or the heterologous expression of PEPc from the cyanobacterium Synechococcus PCC 7002 (Synechococcus) in the CD-P, increased ethylene production (19.2 ± 1.3 and 18.3 ± 3.3 ug mL^-1 OD^-1 day^-1, respectively) when the cells were treated with the acetyl-CoA carboxylase inhibitor, cycloxydim. A heterologous expression of phosphoenolpyruvate synthase (PPSA) from Synechococcus in the CD-P also increased ethylene production (16.77 ± 4.48 ug mL^-1 OD^-1 day^-1) showing differences in the regulation of the native and the PPSA from Synechococcus in Synechocystis. This work demonstrates that genetic rewiring of cyanobacterial central carbon metabolism can enhance carbon supply to the TCA cycle and thereby further increase ethylene production.

Authors:

Durall, Claudia ^[1]; Lindberg, Pia ^[1]; Yu, Jianping ^[2]; Lindbald, Peter ^[1]

Uppsala Univ. (Sweden)
National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Publication Date:: 2020-01-28

Research Org.:: National Renewable Energy Lab. (NREL), Golden, CO (United States)

Sponsoring Org.:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office

OSTI Identifier:: 1598123

Report Number(s):: NREL/JA-2700-74702
Journal ID: ISSN 1754-6834

Grant/Contract Number:: AC36-08GO28308

Resource Type:: Accepted Manuscript

Journal Name:: Biotechnology for Biofuels

Additional Journal Information:: Journal Volume: 13; Journal Issue: 1; Journal ID: ISSN 1754-6834

Publisher:: BioMed Central

Country of Publication:: United States

Language:: English

Subject:: 09 BIOMASS FUELS; ethylene; phosphoenolpyruvate carboxylase; cyanobacterium; Synechocystis; ALPL

Citation Formats


                    Durall, Claudia, Lindberg, Pia, Yu, Jianping, and Lindbald, Peter. Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803.  United States: N. p., 2020. 
        Web.  doi:10.1186/s13068-020-1653-y.

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                    Durall, Claudia, Lindberg, Pia, Yu, Jianping, & Lindbald, Peter. Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803.  United States.  doi:10.1186/s13068-020-1653-y.

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                    Durall, Claudia, Lindberg, Pia, Yu, Jianping, and Lindbald, Peter. Tue .  
        "Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803".  United States.  doi:10.1186/s13068-020-1653-y.  https://www.osti.gov/servlets/purl/1598123.

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@article{osti_1598123,

  title        = {Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803},

  author       = {Durall, Claudia and Lindberg, Pia and Yu, Jianping and Lindbald, Peter},

  abstractNote = {Cyanobacteria can be metabolically engineered to convert CO2 to fuels and chemicals such as ethylene. A major challenge in such efforts is to optimize carbon fixation and partition towards target molecules. The efe gene encoding an ethylene-forming enzyme was introduced into a strain of the cyanobacterium Synechocystis PCC 6803 with increased phosphoenolpyruvate carboxylase (PEPc) levels. The resulting engineered strain (CD-P) showed significantly increased ethylene production (10.5 ± 3.1 ug mL-1 OD-1 day-1) compared to the control strain (6.4 ± 1.4 ug mL-1 OD-1 day-1). Interestingly, extra copies of the native pepc or the heterologous expression of PEPc from the cyanobacterium Synechococcus PCC 7002 (Synechococcus) in the CD-P, increased ethylene production (19.2 ± 1.3 and 18.3 ± 3.3 ug mL-1 OD-1 day-1, respectively) when the cells were treated with the acetyl-CoA carboxylase inhibitor, cycloxydim. A heterologous expression of phosphoenolpyruvate synthase (PPSA) from Synechococcus in the CD-P also increased ethylene production (16.77 ± 4.48 ug mL-1 OD-1 day-1) showing differences in the regulation of the native and the PPSA from Synechococcus in Synechocystis. This work demonstrates that genetic rewiring of cyanobacterial central carbon metabolism can enhance carbon supply to the TCA cycle and thereby further increase ethylene production.},

  doi          = {10.1186/s13068-020-1653-y},

  journal      = {Biotechnology for Biofuels},

  number       = 1,

  volume       = 13,

  place        = {United States},

  year         = {2020},

  month        = {1}

}

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DOI: 10.1186/s13068-020-1653-y

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Works referenced in this record:

Allosteric Inhibition of Phosphoenolpyruvate Carboxylases is Determined by a Single Amino Acid Residue in Cyanobacteria
journal, January 2017

Takeya, Masahiro; Hirai, Masami Yokota; Osanai, Takashi
Scientific Reports, Vol. 7, Issue 1
DOI: 10.1038/srep41080

Ethylene Synthesis and Regulated Expression of Recombinant Protein in Synechocystis sp. PCC 6803
journal, November 2012

Guerrero, Fernando; Carbonell, Verónica; Cossu, Matteo
PLoS ONE, Vol. 7, Issue 11
DOI: 10.1371/journal.pone.0050470

Phototrophic Prokaryotes: The Cyanobacteria
journal, October 1977

Stanier, R. Y.; Bazine, G. C.
Annual Review of Microbiology, Vol. 31, Issue 1
DOI: 10.1146/annurev.mi.31.100177.001301

Citrate synthase from Synechocystis is a distinct class of bacterial citrate synthase
journal, April 2019

Ito, Shoki; Koyama, Naoto; Osanai, Takashi
Scientific Reports, Vol. 9, Issue 1
DOI: 10.1038/s41598-019-42659-z

Metabolic Engineering of Synechocystis sp. PCC 6803 for Production of the Plant Diterpenoid Manoyl Oxide
journal, July 2015

Englund, Elias; Andersen-Ranberg, Johan; Miao, Rui
ACS Synthetic Biology, Vol. 4, Issue 12
DOI: 10.1021/acssynbio.5b00070

Metabolome Phenotyping of Inorganic Carbon Limitation in Cells of the Wild Type and Photorespiratory Mutants of the Cyanobacterium Synechocystis sp. Strain PCC 6803
journal, October 2008

Eisenhut, Marion; Huege, Jan; Schwarz, Doreen
Plant Physiology, Vol. 148, Issue 4
DOI: 10.1104/pp.108.129403

Photosynthetic conversion of carbon dioxide to ethylene by the recombinant cyanobacterium, Synechococcus sp. PCC 7942, which harbors a gene for the ethylene-forming enzyme of Pseudomonas syringae
journal, January 1997

Sakai, Miho; Ogawa, Takahira; Matsuoka, Masayoshi
Journal of Fermentation and Bioengineering, Vol. 84, Issue 5, p. 434-443
DOI: 10.1016/S0922-338X(97)82004-1

Mechanisms of carbon fixation and engineering for increased carbon fixation in cyanobacteria
journal, September 2015

Durall, Claudia; Lindblad, Peter
Algal Research, Vol. 11
DOI: 10.1016/j.algal.2015.07.002

Rubisco: still the most abundant protein of Earth?
journal, February 2013

Raven, John A.
New Phytologist, Vol. 198, Issue 1
DOI: 10.1111/nph.12197

Bioengineering of carbon fixation, biofuels, and biochemicals in cyanobacteria and plants
journal, November 2012

Rosgaard, Lisa; de Porcellinis, Alice Jara; Jacobsen, Jacob H.
Journal of Biotechnology, Vol. 162, Issue 1
DOI: 10.1016/j.jbiotec.2012.05.006

Origin and evolution of circadian clock genes in prokaryotes
journal, February 2003

Dvornyk, V.; Vinogradova, O.; Nevo, E.
Proceedings of the National Academy of Sciences, Vol. 100, Issue 5
DOI: 10.1073/pnas.0130099100

Enhanced growth at low light intensity in the cyanobacterium Synechocystis PCC 6803 by overexpressing phosphoenolpyruvate carboxylase
journal, June 2016

Durall, Claudia; Rukminasari, Nita; Lindblad, Peter
Algal Research, Vol. 16
DOI: 10.1016/j.algal.2016.03.027

CRISPR-Cas9 for the genome engineering of cyanobacteria and succinate production
journal, November 2016

Li, Hung; Shen, Claire R.; Huang, Chun-Hung
Metabolic Engineering, Vol. 38
DOI: 10.1016/j.ymben.2016.09.006

Life and the Evolution of Earth's Atmosphere
journal, May 2002

Kasting, J. F.
Science, Vol. 296, Issue 5570
DOI: 10.1126/science.1071184

Photosynthetic production of the nitrogen-rich compound guanidine
journal, January 2019

Wang, Bo; Dong, Tao; Myrlie, Aldon
Green Chemistry, Vol. 21, Issue 11
DOI: 10.1039/C9GC01003C

Metabolic Changes in Synechocystis PCC6803 upon Nitrogen-Starvation: Excess NADPH Sustains Polyhydroxybutyrate Accumulation
journal, February 2013

Hauf, Waldemar; Schlebusch, Maximilian; Hüge, Jan
Metabolites, Vol. 3, Issue 1
DOI: 10.3390/metabo3010101

Sustained photosynthetic conversion of CO₂ to ethylene in recombinant cyanobacterium Synechocystis 6803
journal, January 2012

Ungerer, Justin; Tao, Ling; Davis, Mark
Energy & Environmental Science, Vol. 5, Issue 10, p. 8998-9006
DOI: 10.1039/c2ee22555g

The plasticity of cyanobacterial metabolism supports direct CO2 conversion to ethylene
journal, April 2015

Xiong, Wei; Morgan, John A.; Ungerer, Justin
Nature Plants, Vol. 1, Issue 5
DOI: 10.1038/nplants.2015.53

Design, Engineering, and Construction of Photosynthetic Microbial Cell Factories for Renewable Solar Fuel Production
journal, March 2012

Lindblad, Peter; Lindberg, Pia; Oliveira, Paulo
AMBIO, Vol. 41, Issue S2
DOI: 10.1007/s13280-012-0274-5

Enhanced stable production of ethylene in photosynthetic cyanobacterium Synechococcus elongatus PCC 7942
journal, May 2019

Carbonell, Veronica; Vuorio, Eerika; Aro, Eva-Mari
World Journal of Microbiology and Biotechnology, Vol. 35, Issue 5
DOI: 10.1007/s11274-019-2652-7

Ethylene production with engineered Synechocystis sp PCC 6803 strains
journal, February 2017

Veetil, Vinod Puthan; Angermayr, S. Andreas; Hellingwerf, Klaas J.
Microbial Cell Factories, Vol. 16, Issue 1
DOI: 10.1186/s12934-017-0645-5

Construction and analysis of a recombinant cyanobacterium expressing a chromosomally inserted gene for an ethylene-forming enzyme at the psbAI locus
journal, January 2003

Takahama, Kazutaka; Matsuoka, Masayoshi; Nagahama, Kazuhiro
Journal of Bioscience and Bioengineering, Vol. 95, Issue 3, p. 302-305
DOI: 10.1016/S1389-1723(03)80034-8

Advances in understanding the cyanobacterial CO2-concentrating-mechanism (CCM): functional components, Ci transporters, diversity, genetic regulation and prospects for engineering into plants
journal, June 2007

Price, G. Dean; Badger, Murray R.; Woodger, Fiona J.
Journal of Experimental Botany, Vol. 59, Issue 7
DOI: 10.1093/jxb/erm112

Notes: Inhibition of the Acetyl-CoA Carboxylase of Barley Chloroplasts by Cycloxydim and Sethoxydim
journal, December 1987

Focke, Manfred; Lichtenthaler, Hartmut K.
Zeitschrift für Naturforschung C, Vol. 42, Issue 11-12
DOI: 10.1515/znc-1987-11-1240

Mapping photoautotrophic metabolism with isotopically nonstationary 13C flux analysis
journal, November 2011

Young, Jamey D.; Shastri, Avantika A.; Stephanopoulos, Gregory
Metabolic Engineering, Vol. 13, Issue 6
DOI: 10.1016/j.ymben.2011.08.002

Interrelation between Cyanophycin Synthesis, L-Arginine Catabolism and Photosynthesis in the Cyanobacterium Synechocystis Sp. Strain PCC 6803
journal, December 2000

Stephan, Dirk Paul; Ruppel, Hans Georg; Pistorius, Elfriede K.
Zeitschrift für Naturforschung C, Vol. 55, Issue 11-12
DOI: 10.1515/znc-2000-11-1214

Engineered cyanobacteria with enhanced growth show increased ethanol production and higher biofuel to biomass ratio
journal, March 2018

Liang, Feiyan; Englund, Elias; Lindberg, Pia
Metabolic Engineering, Vol. 46
DOI: 10.1016/j.ymben.2018.02.006

Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde
journal, November 2009

Atsumi, Shota; Higashide, Wendy; Liao, James C.
Nature Biotechnology, Vol. 27, Issue 12, p. 1177-1180
DOI: 10.1038/nbt.1586

Evaluation of promoters and ribosome binding sites for biotechnological applications in the unicellular cyanobacterium Synechocystis sp. PCC 6803
journal, November 2016

Englund, Elias; Liang, Feiyan; Lindberg, Pia
Scientific Reports, Vol. 6, Issue 1
DOI: 10.1038/srep36640

Metabolic engineering of cyanobacteria for the photosynthetic production of succinate
journal, November 2016

Lan, Ethan I.; Wei, Crystal T.
Metabolic Engineering, Vol. 38
DOI: 10.1016/j.ymben.2016.10.014

Development of suitable photobioreactors for CO₂ sequestration addressing global warming using green algae and cyanobacteria
journal, April 2011

Kumar, Kanhaiya; Dasgupta, Chitralekha Nag; Nayak, Bikram
Bioresource Technology, Vol. 102, Issue 8, p. 4945-4953
DOI: 10.1016/j.biortech.2011.01.054

Global metabolic rewiring for improved CO2 fixation and chemical production in cyanobacteria
journal, March 2017

Kanno, Masahiro; Carroll, Austin L.; Atsumi, Shota
Nature Communications, Vol. 8, Issue 1
DOI: 10.1038/ncomms14724

Enhancing photosynthetic production of ethylene in genetically engineered Synechocystis sp. PCC 6803
journal, January 2015

Zhu, Tao; Xie, Xiaoman; Li, Zhimin
Green Chemistry, Vol. 17, Issue 1
DOI: 10.1039/C4GC01730G

Regulation of glutamine synthetase activity in the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 by the nitrogen source: effect of ammonium.
journal, July 1991

Mérida, A.; Candau, P.; Florencio, F. J.
Journal of Bacteriology, Vol. 173, Issue 13
DOI: 10.1128/JB.173.13.4095-4100.1991

Introduction of a Synthetic CO ₂ -fixing Photorespiratory Bypass into a Cyanobacterium
journal, February 2014

Shih, Patrick M.; Zarzycki, Jan; Niyogi, Krishna K.
Journal of Biological Chemistry, Vol. 289, Issue 14
DOI: 10.1074/jbc.C113.543132

Design and analysis of synthetic carbon fixation pathways
journal, April 2010

Bar-Even, A.; Noor, E.; Lewis, N. E.
Proceedings of the National Academy of Sciences, Vol. 107, Issue 19, p. 8889-8894
DOI: 10.1073/pnas.0907176107

Ethylene Biosynthesis
journal, June 1993

Kende, H.
Annual Review of Plant Physiology and Plant Molecular Biology, Vol. 44, Issue 1
DOI: 10.1146/annurev.pp.44.060193.001435

Comparison of Ethylene Production by Pseudomonas syringae and Ralstonia solanacearum
journal, May 1999

Weingart, H.; Völksch, B.; Ullrich, M. S.
Phytopathology, Vol. 89, Issue 5
DOI: 10.1094/PHYTO.1999.89.5.360

Ethylene-forming enzyme and bioethylene production
journal, January 2014

Eckert, Carrie; Xu, Wu; Xiong, Wei
Biotechnology for Biofuels, Vol. 7, Issue 1
DOI: 10.1186/1754-6834-7-33

Membrane-Inlet Mass Spectrometry Enables a Quantitative Understanding of Inorganic Carbon Uptake Flux and Carbon Concentrating Mechanisms in Metabolically Engineered Cyanobacteria
journal, June 2019

Douchi, Damien; Liang, Feiyan; Cano, Melissa
Frontiers in Microbiology, Vol. 10
DOI: 10.3389/fmicb.2019.01356

Enhancement of photosynthetic isobutanol production in engineered cells of Synechocystis PCC 6803
journal, September 2018

Miao, Rui; Xie, Hao; Lindblad, Peter
Biotechnology for Biofuels, Vol. 11, Issue 1
DOI: 10.1186/s13068-018-1268-8

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Title: Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803

Abstract

Citation Formats

Allosteric Inhibition of Phosphoenolpyruvate Carboxylases is Determined by a Single Amino Acid Residue in Cyanobacteria journal, January 2017

Ethylene Synthesis and Regulated Expression of Recombinant Protein in Synechocystis sp. PCC 6803 journal, November 2012

Phototrophic Prokaryotes: The Cyanobacteria journal, October 1977

Citrate synthase from Synechocystis is a distinct class of bacterial citrate synthase journal, April 2019

Metabolic Engineering of Synechocystis sp. PCC 6803 for Production of the Plant Diterpenoid Manoyl Oxide journal, July 2015

Metabolome Phenotyping of Inorganic Carbon Limitation in Cells of the Wild Type and Photorespiratory Mutants of the Cyanobacterium Synechocystis sp. Strain PCC 6803 journal, October 2008

Photosynthetic conversion of carbon dioxide to ethylene by the recombinant cyanobacterium, Synechococcus sp. PCC 7942, which harbors a gene for the ethylene-forming enzyme of Pseudomonas syringae journal, January 1997

Mechanisms of carbon fixation and engineering for increased carbon fixation in cyanobacteria journal, September 2015

Rubisco: still the most abundant protein of Earth? journal, February 2013

Bioengineering of carbon fixation, biofuels, and biochemicals in cyanobacteria and plants journal, November 2012

Origin and evolution of circadian clock genes in prokaryotes journal, February 2003

Enhanced growth at low light intensity in the cyanobacterium Synechocystis PCC 6803 by overexpressing phosphoenolpyruvate carboxylase journal, June 2016

CRISPR-Cas9 for the genome engineering of cyanobacteria and succinate production journal, November 2016

Life and the Evolution of Earth's Atmosphere journal, May 2002

Photosynthetic production of the nitrogen-rich compound guanidine journal, January 2019

Metabolic Changes in Synechocystis PCC6803 upon Nitrogen-Starvation: Excess NADPH Sustains Polyhydroxybutyrate Accumulation journal, February 2013

Sustained photosynthetic conversion of CO2 to ethylene in recombinant cyanobacterium Synechocystis 6803 journal, January 2012

The plasticity of cyanobacterial metabolism supports direct CO2 conversion to ethylene journal, April 2015

Design, Engineering, and Construction of Photosynthetic Microbial Cell Factories for Renewable Solar Fuel Production journal, March 2012

Enhanced stable production of ethylene in photosynthetic cyanobacterium Synechococcus elongatus PCC 7942 journal, May 2019

Ethylene production with engineered Synechocystis sp PCC 6803 strains journal, February 2017

Construction and analysis of a recombinant cyanobacterium expressing a chromosomally inserted gene for an ethylene-forming enzyme at the psbAI locus journal, January 2003

Advances in understanding the cyanobacterial CO2-concentrating-mechanism (CCM): functional components, Ci transporters, diversity, genetic regulation and prospects for engineering into plants journal, June 2007

Notes: Inhibition of the Acetyl-CoA Carboxylase of Barley Chloroplasts by Cycloxydim and Sethoxydim journal, December 1987

Mapping photoautotrophic metabolism with isotopically nonstationary 13C flux analysis journal, November 2011

Interrelation between Cyanophycin Synthesis, L-Arginine Catabolism and Photosynthesis in the Cyanobacterium Synechocystis Sp. Strain PCC 6803 journal, December 2000

Engineered cyanobacteria with enhanced growth show increased ethanol production and higher biofuel to biomass ratio journal, March 2018

Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde journal, November 2009

Evaluation of promoters and ribosome binding sites for biotechnological applications in the unicellular cyanobacterium Synechocystis sp. PCC 6803 journal, November 2016

Metabolic engineering of cyanobacteria for the photosynthetic production of succinate journal, November 2016

Development of suitable photobioreactors for CO2 sequestration addressing global warming using green algae and cyanobacteria journal, April 2011

Global metabolic rewiring for improved CO2 fixation and chemical production in cyanobacteria journal, March 2017

Enhancing photosynthetic production of ethylene in genetically engineered Synechocystis sp. PCC 6803 journal, January 2015

Regulation of glutamine synthetase activity in the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 by the nitrogen source: effect of ammonium. journal, July 1991

Introduction of a Synthetic CO 2 -fixing Photorespiratory Bypass into a Cyanobacterium journal, February 2014

Design and analysis of synthetic carbon fixation pathways journal, April 2010

Ethylene Biosynthesis journal, June 1993

Comparison of Ethylene Production by Pseudomonas syringae and Ralstonia solanacearum journal, May 1999

Ethylene-forming enzyme and bioethylene production journal, January 2014

Membrane-Inlet Mass Spectrometry Enables a Quantitative Understanding of Inorganic Carbon Uptake Flux and Carbon Concentrating Mechanisms in Metabolically Engineered Cyanobacteria journal, June 2019

Enhancement of photosynthetic isobutanol production in engineered cells of Synechocystis PCC 6803 journal, September 2018

Allosteric Inhibition of Phosphoenolpyruvate Carboxylases is Determined by a Single Amino Acid Residue in Cyanobacteria
journal, January 2017

Ethylene Synthesis and Regulated Expression of Recombinant Protein in Synechocystis sp. PCC 6803
journal, November 2012

Phototrophic Prokaryotes: The Cyanobacteria
journal, October 1977

Citrate synthase from Synechocystis is a distinct class of bacterial citrate synthase
journal, April 2019

Metabolic Engineering of Synechocystis sp. PCC 6803 for Production of the Plant Diterpenoid Manoyl Oxide
journal, July 2015

Metabolome Phenotyping of Inorganic Carbon Limitation in Cells of the Wild Type and Photorespiratory Mutants of the Cyanobacterium Synechocystis sp. Strain PCC 6803
journal, October 2008

Photosynthetic conversion of carbon dioxide to ethylene by the recombinant cyanobacterium, Synechococcus sp. PCC 7942, which harbors a gene for the ethylene-forming enzyme of Pseudomonas syringae
journal, January 1997

Mechanisms of carbon fixation and engineering for increased carbon fixation in cyanobacteria
journal, September 2015

Rubisco: still the most abundant protein of Earth?
journal, February 2013

Bioengineering of carbon fixation, biofuels, and biochemicals in cyanobacteria and plants
journal, November 2012

Origin and evolution of circadian clock genes in prokaryotes
journal, February 2003

Enhanced growth at low light intensity in the cyanobacterium Synechocystis PCC 6803 by overexpressing phosphoenolpyruvate carboxylase
journal, June 2016

CRISPR-Cas9 for the genome engineering of cyanobacteria and succinate production
journal, November 2016

Life and the Evolution of Earth's Atmosphere
journal, May 2002

Photosynthetic production of the nitrogen-rich compound guanidine
journal, January 2019

Metabolic Changes in Synechocystis PCC6803 upon Nitrogen-Starvation: Excess NADPH Sustains Polyhydroxybutyrate Accumulation
journal, February 2013

Sustained photosynthetic conversion of CO₂ to ethylene in recombinant cyanobacterium Synechocystis 6803
journal, January 2012

The plasticity of cyanobacterial metabolism supports direct CO2 conversion to ethylene
journal, April 2015

Design, Engineering, and Construction of Photosynthetic Microbial Cell Factories for Renewable Solar Fuel Production
journal, March 2012

Enhanced stable production of ethylene in photosynthetic cyanobacterium Synechococcus elongatus PCC 7942
journal, May 2019

Ethylene production with engineered Synechocystis sp PCC 6803 strains
journal, February 2017

Construction and analysis of a recombinant cyanobacterium expressing a chromosomally inserted gene for an ethylene-forming enzyme at the psbAI locus
journal, January 2003

Advances in understanding the cyanobacterial CO2-concentrating-mechanism (CCM): functional components, Ci transporters, diversity, genetic regulation and prospects for engineering into plants
journal, June 2007

Notes: Inhibition of the Acetyl-CoA Carboxylase of Barley Chloroplasts by Cycloxydim and Sethoxydim
journal, December 1987

Mapping photoautotrophic metabolism with isotopically nonstationary 13C flux analysis
journal, November 2011

Interrelation between Cyanophycin Synthesis, L-Arginine Catabolism and Photosynthesis in the Cyanobacterium Synechocystis Sp. Strain PCC 6803
journal, December 2000

Engineered cyanobacteria with enhanced growth show increased ethanol production and higher biofuel to biomass ratio
journal, March 2018

Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde
journal, November 2009

Evaluation of promoters and ribosome binding sites for biotechnological applications in the unicellular cyanobacterium Synechocystis sp. PCC 6803
journal, November 2016

Metabolic engineering of cyanobacteria for the photosynthetic production of succinate
journal, November 2016

Development of suitable photobioreactors for CO₂ sequestration addressing global warming using green algae and cyanobacteria
journal, April 2011

Global metabolic rewiring for improved CO2 fixation and chemical production in cyanobacteria
journal, March 2017

Enhancing photosynthetic production of ethylene in genetically engineered Synechocystis sp. PCC 6803
journal, January 2015

Regulation of glutamine synthetase activity in the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 by the nitrogen source: effect of ammonium.
journal, July 1991

Introduction of a Synthetic CO ₂ -fixing Photorespiratory Bypass into a Cyanobacterium
journal, February 2014

Design and analysis of synthetic carbon fixation pathways
journal, April 2010

Ethylene Biosynthesis
journal, June 1993

Comparison of Ethylene Production by Pseudomonas syringae and Ralstonia solanacearum
journal, May 1999

Ethylene-forming enzyme and bioethylene production
journal, January 2014

Membrane-Inlet Mass Spectrometry Enables a Quantitative Understanding of Inorganic Carbon Uptake Flux and Carbon Concentrating Mechanisms in Metabolically Engineered Cyanobacteria
journal, June 2019

Enhancement of photosynthetic isobutanol production in engineered cells of Synechocystis PCC 6803
journal, September 2018