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Title: TrkA undergoes a tetramer-to-dimer conversion to open TrkH which enables changes in membrane potential

Abstract

TrkH is a bacterial ion channel implicated in K+ uptake and pH regulation. TrkH assembles with its regulatory protein, TrkA, which closes the channel when bound to ADP and opens it when bound to ATP. However, it is unknown how nucleotides control the gating of TrkH through TrkA. Here we report the structures of the TrkH-TrkA complex in the presence of ADP or ATP. TrkA forms a tetrameric ring when bound to ADP and constrains TrkH to a closed conformation. The TrkA ring splits into two TrkA dimers in the presence of ATP and releases the constraints on TrkH, resulting in an open channel conformation. Functional studies show that both the tetramer-to-dimer conversion of TrkA and the loss of constraints on TrkH are required for channel gating. In addition, deletion of TrkA in Escherichia coli depolarizes the cell, suggesting that the TrkH-TrkA complex couples changes in intracellular nucleotides to membrane potential.

Authors:
 [1];  [1]; ORCiD logo [1];  [2];  [2];  [1];  [1]; ORCiD logo [1]; ORCiD logo [1];  [2];  [3];  [1]
  1. Baylor College of Medicine, Houston, TX (United States)
  2. Texas A & M Univ., College Station, TX (United States)
  3. Stanford Univ., CA (United States)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
National Institutes of Health (NIH); Cancer Prevention and Research Institute of Texas (CPRIT); Robert Welch Foundation
OSTI Identifier:
1598012
Grant/Contract Number:  
HL086392; DK122784; P41GM103832; GM132436; R1223; Q-1279; Q-1967; A-1742; AC02-06CH11357
Resource Type:
Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 11; Journal Issue: 1; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
ENGLISH
Subject:
36 MATERIALS SCIENCE; Cryoelectron microscopy; Permeation and transport; X-ray crystallography

Citation Formats

Zhang, Hanzhi, Pan, Yaping, Hu, Liya, Hudson, M. Ashley, Hofstetter, Katrina S., Xu, Zhichun, Rong, Mingqiang, Wang, Zhao, Prasad, B. V. Venkataram, Lockless, Steve W., Chiu, Wah, and Zhou, Ming. TrkA undergoes a tetramer-to-dimer conversion to open TrkH which enables changes in membrane potential. United States: N. p., 2020. Web. doi:10.1038/s41467-019-14240-9.
Zhang, Hanzhi, Pan, Yaping, Hu, Liya, Hudson, M. Ashley, Hofstetter, Katrina S., Xu, Zhichun, Rong, Mingqiang, Wang, Zhao, Prasad, B. V. Venkataram, Lockless, Steve W., Chiu, Wah, & Zhou, Ming. TrkA undergoes a tetramer-to-dimer conversion to open TrkH which enables changes in membrane potential. United States. https://doi.org/10.1038/s41467-019-14240-9
Zhang, Hanzhi, Pan, Yaping, Hu, Liya, Hudson, M. Ashley, Hofstetter, Katrina S., Xu, Zhichun, Rong, Mingqiang, Wang, Zhao, Prasad, B. V. Venkataram, Lockless, Steve W., Chiu, Wah, and Zhou, Ming. Tue . "TrkA undergoes a tetramer-to-dimer conversion to open TrkH which enables changes in membrane potential". United States. https://doi.org/10.1038/s41467-019-14240-9. https://www.osti.gov/servlets/purl/1598012.
@article{osti_1598012,
title = {TrkA undergoes a tetramer-to-dimer conversion to open TrkH which enables changes in membrane potential},
author = {Zhang, Hanzhi and Pan, Yaping and Hu, Liya and Hudson, M. Ashley and Hofstetter, Katrina S. and Xu, Zhichun and Rong, Mingqiang and Wang, Zhao and Prasad, B. V. Venkataram and Lockless, Steve W. and Chiu, Wah and Zhou, Ming},
abstractNote = {TrkH is a bacterial ion channel implicated in K+ uptake and pH regulation. TrkH assembles with its regulatory protein, TrkA, which closes the channel when bound to ADP and opens it when bound to ATP. However, it is unknown how nucleotides control the gating of TrkH through TrkA. Here we report the structures of the TrkH-TrkA complex in the presence of ADP or ATP. TrkA forms a tetrameric ring when bound to ADP and constrains TrkH to a closed conformation. The TrkA ring splits into two TrkA dimers in the presence of ATP and releases the constraints on TrkH, resulting in an open channel conformation. Functional studies show that both the tetramer-to-dimer conversion of TrkA and the loss of constraints on TrkH are required for channel gating. In addition, deletion of TrkA in Escherichia coli depolarizes the cell, suggesting that the TrkH-TrkA complex couples changes in intracellular nucleotides to membrane potential.},
doi = {10.1038/s41467-019-14240-9},
journal = {Nature Communications},
number = 1,
volume = 11,
place = {United States},
year = {2020},
month = {1}
}

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Structure of the glucagon receptor in complex with a glucagon analogue
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Structural basis of ligand binding modes at the neuropeptide Y Y1 receptor
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Structure of Gremlin-1 and analysis of its interaction with BMP-2
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Specific keratinase derived designer peptides potently inhibit Aβ aggregation resulting in reduced neuronal toxicity and apoptosis
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Investigation of specific binding of designed oligodeoxynucleotide decoys to transcription factors in HT29 cell line undergoing epithelial–mesenchymal transition (EMT)
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Thienopyrimidine sulphonamide hybrids: design, synthesis, antiprotozoal activity and molecular docking studies
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D224V and S128Y mutation in FSHR ED influence thumb movement differentially: An intricate insight gained by short‐term molecular dynamics simulation
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Deciphering the binding behavior of flavonoids to the cyclin dependent kinase 6/cyclin D complex
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Human VP8* mAbs neutralize rotavirus selectively in human intestinal epithelial cells
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Lysozyme's lectin-like characteristics facilitates its immune defense function
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Microencapsulation of Anthocyanins from Grape Skins by Whey Protein Isolates and Different Polymers
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Defining ICR-Mo, an intrinsic colistin resistance determinant from Moraxella osloensis
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First structure of archaeal branched-chain amino acid aminotransferase from Thermoproteus uzoniensis specific for l-amino acids and R-amines
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Novel calcitriol analogue with an oxolane group: In vitro, in vivo, and in silico studies
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Insight into microtubule destabilization mechanism of 3,4,5-trimethoxyphenyl indanone derivatives using molecular dynamics simulation and conformational modes analysis
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X-ray structure of Fasciola hepatica Sigma class glutathione transferase 1 reveals a disulfide bond to support stability in gastro-intestinal environment
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UDP-Glucose 4-Epimerase and β-1,4-Galactosyltransferase from the Oyster Magallana gigas as Valuable Biocatalysts for the Production of Galactosylated Products
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Insights into cytochrome bc1 complex binding mode of antimalarial 2-hydroxy-1,4-naphthoquinones through molecular modelling
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High throughput in silico identification and characterization of Plasmodium falciparum PRL phosphatase inhibitors
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Comparative structural analysis of the caspase family with other clan CD cysteine peptidases
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The intrinsic structure of poly(A) RNA determines the specificity of Pan2 and Caf1 deadenylases
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