Preferential interactions of primary amine-terminated quantum dots with membrane domain boundaries and lipid rafts revealed with nanometer resolution
Abstract
The initial interactions of engineered nanoparticles (NPs) with living cells are governed by physicochemical properties of the NP and the molecular composition and structure of the cell membrane. Eukaryotic cell membranes contain lipid rafts – liquid-ordered nanodomains involved in membrane trafficking and molecular signaling. However, the impact of these membrane structures on cellular interactions of NPs remains unclear. Here we investigate the role of membrane domains in the interactions of primary amine-terminated quantum dots (Qdots) with liquid-ordered domains or lipid rafts in model membranes and intact cells, respectively. Using correlative atomic force and fluorescence microscopy, we found that the Qdots preferentially localized to boundaries between liquid-ordered and liquid-disordered phases in supported bilayers. The Qdots also induced holes at these phase boundaries. Using super resolution fluorescence microscopy (STORM), we found that the Qdots preferentially co-localized with lipid rafts in the membrane of intact trout gill epithelial cells – a model cell type for environmental exposures. Our observations uncovered preferential interactions of amine-terminated Qdots with liquid-ordered domains and their boundaries, possibly due to membrane curvature at phase boundaries creating energetically favorable sites for NP interactions. The preferential interaction of the Qdots with lipid rafts supports their potential internalization via lipid raft-mediated endocytosismore »
- Authors:
-
[1];
[1];
[1];
[2];
[2];
[1]
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
- Univ. of Wisconsin, Milwaukee, WI (United States). Dept. of Chemistry
- Publication Date:
- Research Org.:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1597658
- Report Number(s):
- PNNL-SA-146870
Journal ID: ISSN 2051-8153; ESNNA4
- Grant/Contract Number:
- AC05-76RL01830
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Environmental Science: Nano
- Additional Journal Information:
- Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 2051-8153
- Publisher:
- Royal Society of Chemistry
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 77 NANOSCIENCE AND NANOTECHNOLOGY
Citation Formats
Mensch, Arielle C., Melby, Eric S., Laudadio, Elizabeth D., Foreman-Ortiz, Isabel U., Zhang, Yongqian, Dohnalkova, Alice, Hu, Dehong, Pedersen, Joel A., Hamers, Robert J., and Orr, Galya. Preferential interactions of primary amine-terminated quantum dots with membrane domain boundaries and lipid rafts revealed with nanometer resolution. United States: N. p., 2019.
Web. doi:10.1039/C9EN00996E.
Mensch, Arielle C., Melby, Eric S., Laudadio, Elizabeth D., Foreman-Ortiz, Isabel U., Zhang, Yongqian, Dohnalkova, Alice, Hu, Dehong, Pedersen, Joel A., Hamers, Robert J., & Orr, Galya. Preferential interactions of primary amine-terminated quantum dots with membrane domain boundaries and lipid rafts revealed with nanometer resolution. United States. https://doi.org/10.1039/C9EN00996E
Mensch, Arielle C., Melby, Eric S., Laudadio, Elizabeth D., Foreman-Ortiz, Isabel U., Zhang, Yongqian, Dohnalkova, Alice, Hu, Dehong, Pedersen, Joel A., Hamers, Robert J., and Orr, Galya. Mon .
"Preferential interactions of primary amine-terminated quantum dots with membrane domain boundaries and lipid rafts revealed with nanometer resolution". United States. https://doi.org/10.1039/C9EN00996E. https://www.osti.gov/servlets/purl/1597658.
@article{osti_1597658,
title = {Preferential interactions of primary amine-terminated quantum dots with membrane domain boundaries and lipid rafts revealed with nanometer resolution},
author = {Mensch, Arielle C. and Melby, Eric S. and Laudadio, Elizabeth D. and Foreman-Ortiz, Isabel U. and Zhang, Yongqian and Dohnalkova, Alice and Hu, Dehong and Pedersen, Joel A. and Hamers, Robert J. and Orr, Galya},
abstractNote = {The initial interactions of engineered nanoparticles (NPs) with living cells are governed by physicochemical properties of the NP and the molecular composition and structure of the cell membrane. Eukaryotic cell membranes contain lipid rafts – liquid-ordered nanodomains involved in membrane trafficking and molecular signaling. However, the impact of these membrane structures on cellular interactions of NPs remains unclear. Here we investigate the role of membrane domains in the interactions of primary amine-terminated quantum dots (Qdots) with liquid-ordered domains or lipid rafts in model membranes and intact cells, respectively. Using correlative atomic force and fluorescence microscopy, we found that the Qdots preferentially localized to boundaries between liquid-ordered and liquid-disordered phases in supported bilayers. The Qdots also induced holes at these phase boundaries. Using super resolution fluorescence microscopy (STORM), we found that the Qdots preferentially co-localized with lipid rafts in the membrane of intact trout gill epithelial cells – a model cell type for environmental exposures. Our observations uncovered preferential interactions of amine-terminated Qdots with liquid-ordered domains and their boundaries, possibly due to membrane curvature at phase boundaries creating energetically favorable sites for NP interactions. The preferential interaction of the Qdots with lipid rafts supports their potential internalization via lipid raft-mediated endocytosis and interactions with raft-resident signaling molecules.},
doi = {10.1039/C9EN00996E},
journal = {Environmental Science: Nano},
number = 1,
volume = 7,
place = {United States},
year = {2019},
month = {12}
}
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