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Title: CAMTA-Mediated Regulation of Salicylic Acid Immunity Pathway Genes in Arabidopsis Exposed to Low Temperature and Pathogen Infection

Abstract

Arabidopsis thaliana calmodulin binding transcription activator (CAMTA) factors repress the expression of genes involved in salicylic acid (SA) biosynthesis and SA-mediated immunity in healthy plants grown at warm temperature (22°C). This repression is overcome in plants exposed to low temperature (4°C) for more than a week and in plants infected by biotrophic and hemibiotrophic pathogens. Here, we present evidence that CAMTA3-mediated repression of SA pathway genes in nonstressed plants involves the action of an N-terminal repression module (NRM) that acts independently of calmodulin (CaM) binding to the IQ and CaM binding (CaMB) domains, a finding that is contrary to current thinking that CAMTA3 repression activity requires binding of CaM to the CaMB domain. Induction of SA pathway genes in response to low temperature did not occur in plants expressing only the CAMTA3-NRM region of the protein. Mutational analysis provided evidence that the repression activity of the NRM was suppressed by action of the IQ and CaMB domains responding to signals generated in response to low temperature. Plants expressing the CAMTA3-NRM region were also impaired in defense against the bacterial hemibiotrophic pathogen Pseudomonas syringae pv tomato DC3000. Our results indicate that the regulation of CAMTA3 repression activity by low temperature andmore » pathogen infection involves related mechanisms, but with distinct differences.« less

Authors:
ORCiD logo [1]; ORCiD logo [1];  [1];  [1];  [2];  [1]; ORCiD logo [1]; ORCiD logo [3]; ORCiD logo [1]
  1. Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory
  2. Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory; Nanjing Agricultural Univ. (China)
  3. Department of Horticulture, Michigan State University, East Lansing, Michigan 48824
Publication Date:
Research Org.:
Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory
Sponsoring Org.:
USDOE Office of Science, SC Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1596269
Grant/Contract Number:  
[FG02-91ER20021]
Resource Type:
Accepted Manuscript
Journal Name:
Plant Cell
Additional Journal Information:
[ Journal Volume: 29; Journal Issue: 10]; Journal ID: ISSN 1040-4651
Publisher:
American Society of Plant Biologists
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Kim, Yong Sig, An, Chuanfu, Park, Sunchung, Gilmour, Sarah J., Wang, Ling, Renna, Luciana, Brandizzi, Federica, Grumet, Rebecca, and Thomashow, Michael F. CAMTA-Mediated Regulation of Salicylic Acid Immunity Pathway Genes in Arabidopsis Exposed to Low Temperature and Pathogen Infection. United States: N. p., 2017. Web. doi:10.1105/tpc.16.00865.
Kim, Yong Sig, An, Chuanfu, Park, Sunchung, Gilmour, Sarah J., Wang, Ling, Renna, Luciana, Brandizzi, Federica, Grumet, Rebecca, & Thomashow, Michael F. CAMTA-Mediated Regulation of Salicylic Acid Immunity Pathway Genes in Arabidopsis Exposed to Low Temperature and Pathogen Infection. United States. doi:10.1105/tpc.16.00865.
Kim, Yong Sig, An, Chuanfu, Park, Sunchung, Gilmour, Sarah J., Wang, Ling, Renna, Luciana, Brandizzi, Federica, Grumet, Rebecca, and Thomashow, Michael F. Sun . "CAMTA-Mediated Regulation of Salicylic Acid Immunity Pathway Genes in Arabidopsis Exposed to Low Temperature and Pathogen Infection". United States. doi:10.1105/tpc.16.00865. https://www.osti.gov/servlets/purl/1596269.
@article{osti_1596269,
title = {CAMTA-Mediated Regulation of Salicylic Acid Immunity Pathway Genes in Arabidopsis Exposed to Low Temperature and Pathogen Infection},
author = {Kim, Yong Sig and An, Chuanfu and Park, Sunchung and Gilmour, Sarah J. and Wang, Ling and Renna, Luciana and Brandizzi, Federica and Grumet, Rebecca and Thomashow, Michael F.},
abstractNote = {Arabidopsis thaliana calmodulin binding transcription activator (CAMTA) factors repress the expression of genes involved in salicylic acid (SA) biosynthesis and SA-mediated immunity in healthy plants grown at warm temperature (22°C). This repression is overcome in plants exposed to low temperature (4°C) for more than a week and in plants infected by biotrophic and hemibiotrophic pathogens. Here, we present evidence that CAMTA3-mediated repression of SA pathway genes in nonstressed plants involves the action of an N-terminal repression module (NRM) that acts independently of calmodulin (CaM) binding to the IQ and CaM binding (CaMB) domains, a finding that is contrary to current thinking that CAMTA3 repression activity requires binding of CaM to the CaMB domain. Induction of SA pathway genes in response to low temperature did not occur in plants expressing only the CAMTA3-NRM region of the protein. Mutational analysis provided evidence that the repression activity of the NRM was suppressed by action of the IQ and CaMB domains responding to signals generated in response to low temperature. Plants expressing the CAMTA3-NRM region were also impaired in defense against the bacterial hemibiotrophic pathogen Pseudomonas syringae pv tomato DC3000. Our results indicate that the regulation of CAMTA3 repression activity by low temperature and pathogen infection involves related mechanisms, but with distinct differences.},
doi = {10.1105/tpc.16.00865},
journal = {Plant Cell},
number = [10],
volume = [29],
place = {United States},
year = {2017},
month = {10}
}

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