Protein coating composition targets nanoparticles to leaf stomata and trichomes
Abstract
Plant nanobiotechnology has the potential to revolutionize agriculture. However, the lack of effective methods to deliver nanoparticles (NPs) to the precise locations in plants where they are needed impedes these technological innovations. Here, model gold nanoparticles (AuNP) were coated with citrate, bovine serum albumin (BSA) as a protein control, or LM6-M, an antibody with an affinity for functional groups unique to stomata on leaf surfaces to deliver the AuNPs to stomata. One-month-old Vicia faba leaves were exposed via drop deposition to aqueous suspensions of LM6-M-coated AuNPs and allowed to air dry. After rinsing, Au distribution on the leaf surface was investigated by enhanced dark-field microscopy and X-ray fluorescence mapping. While citrate-coated AuNPs randomly covered the plant leaves, LM6M-AuNPs strongly adhered to the stomata and remained on the leaf surface after rinsing, and BSA-AuNPs specifically targeted trichome hairs. To the authors’ knowledge, this is the first report of active targeting of live leaf structures using NPs coated with molecular recognition molecules. Finally, this proof-of-concept study provides a strategy for future targeted nanopesticide delivery research.
- Authors:
-
[1];
[1];
[1];
[1];
[2];
[3];
[1]
- Carnegie Mellon Univ., Pittsburgh, PA (United States)
- Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source II (NSLS-II)
- Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source II (NSLS-II); Univ. of Chicago, IL (United States)
- Publication Date:
- Research Org.:
- Brookhaven National Laboratory (BNL), Upton, NY (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1607976
- Alternate Identifier(s):
- OSTI ID: 1596172
- Report Number(s):
- BNL-213784-2020-JAAM
Journal ID: ISSN 2040-3364; NANOHL
- Grant/Contract Number:
- SC0012704; FG-02-92ER14244
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nanoscale
- Additional Journal Information:
- Journal Volume: 12; Journal Issue: 6; Journal ID: ISSN 2040-3364
- Publisher:
- Royal Society of Chemistry
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES
Citation Formats
Spielman-Sun, Eleanor, Avellan, Astrid, Bland, Garret D., Clement, Emma T., Tappero, Ryan V., Acerbo, Alvin S., and Lowry, Gregory V. Protein coating composition targets nanoparticles to leaf stomata and trichomes. United States: N. p., 2020.
Web. doi:10.1039/C9NR08100C.
Spielman-Sun, Eleanor, Avellan, Astrid, Bland, Garret D., Clement, Emma T., Tappero, Ryan V., Acerbo, Alvin S., & Lowry, Gregory V. Protein coating composition targets nanoparticles to leaf stomata and trichomes. United States. https://doi.org/10.1039/C9NR08100C
Spielman-Sun, Eleanor, Avellan, Astrid, Bland, Garret D., Clement, Emma T., Tappero, Ryan V., Acerbo, Alvin S., and Lowry, Gregory V. Fri .
"Protein coating composition targets nanoparticles to leaf stomata and trichomes". United States. https://doi.org/10.1039/C9NR08100C. https://www.osti.gov/servlets/purl/1607976.
@article{osti_1607976,
title = {Protein coating composition targets nanoparticles to leaf stomata and trichomes},
author = {Spielman-Sun, Eleanor and Avellan, Astrid and Bland, Garret D. and Clement, Emma T. and Tappero, Ryan V. and Acerbo, Alvin S. and Lowry, Gregory V.},
abstractNote = {Plant nanobiotechnology has the potential to revolutionize agriculture. However, the lack of effective methods to deliver nanoparticles (NPs) to the precise locations in plants where they are needed impedes these technological innovations. Here, model gold nanoparticles (AuNP) were coated with citrate, bovine serum albumin (BSA) as a protein control, or LM6-M, an antibody with an affinity for functional groups unique to stomata on leaf surfaces to deliver the AuNPs to stomata. One-month-old Vicia faba leaves were exposed via drop deposition to aqueous suspensions of LM6-M-coated AuNPs and allowed to air dry. After rinsing, Au distribution on the leaf surface was investigated by enhanced dark-field microscopy and X-ray fluorescence mapping. While citrate-coated AuNPs randomly covered the plant leaves, LM6M-AuNPs strongly adhered to the stomata and remained on the leaf surface after rinsing, and BSA-AuNPs specifically targeted trichome hairs. To the authors’ knowledge, this is the first report of active targeting of live leaf structures using NPs coated with molecular recognition molecules. Finally, this proof-of-concept study provides a strategy for future targeted nanopesticide delivery research.},
doi = {10.1039/C9NR08100C},
journal = {Nanoscale},
number = 6,
volume = 12,
place = {United States},
year = {Fri Jan 17 00:00:00 EST 2020},
month = {Fri Jan 17 00:00:00 EST 2020}
}
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