skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

This content will become publicly available on September 18, 2020

Title: Energy Conservation and Hydrogenase Function in Methanogenic Archaea, in Particular the Genus Methanosarcina

Abstract

The biological production of methane is vital to the global carbon cycle and accounts for ca. 74% of total methane emissions. The organisms that facilitate this process, methanogenic archaea, belong to a large and phylogenetically diverse group that thrives in a wide range of anaerobic environments. Two main subgroups exist within methanogenic archaea: those with and those without cytochromes. Although a variety of metabolisms exist within this group, the reduction of growth substrates to methane using electrons from molecular hydrogen is, in a phylogenetic sense, the most widespread methanogenic pathway. Methanogens without cytochromes typically generate methane by the reduction of CO 2 with electrons derived from H 2, formate, or secondary alcohols, generating a transmembrane ion gradient for ATP production via an Na +-translocating methyltransferase (Mtr). These organisms also conserve energy with a novel flavin-based electron bifurcation mechanism, wherein the endergonic reduction of ferredoxin is facilitated by the exergonic reduction of a disulfide terminal electron acceptor coupled to either H 2 or formate oxidation. Methanogens that utilize cytochromes have a broader substrate range, and can convert acetate and methylated compounds to methane, in addition to the ability to reduce CO 2. Cytochrome-containing methanogens are able to supplement the ion motivemore » force generated by Mtr with an H +-translocating electron transport system. In both groups, enzymes known as hydrogenases, which reversibly interconvert protons and electrons to molecular hydrogen, play a central role in the methanogenic process. In this review, we discuss recent insight into methanogen metabolism and energy conservation mechanisms with a particular focus on the genus Methanosarcina.« less

Authors:
 [1]; ORCiD logo [1]
  1. Univ. of Illinois at Urbana-Champaign, IL (United States)
Publication Date:
Research Org.:
William W. Metcalf (University of Illinois at Urbana-Champaign
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Chemical Sciences, Geosciences & Biosciences Division
OSTI Identifier:
1595766
Grant/Contract Number:  
FG02-02ER15296
Resource Type:
Accepted Manuscript
Journal Name:
Microbiology and Molecular Biology Reviews
Additional Journal Information:
Journal Volume: 83; Journal Issue: 4; Journal ID: ISSN 1092-2172
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; archaea; energy conservation; hydrogenase; methanogenesis

Citation Formats

Mand, Thomas D., and Metcalf, William W. Energy Conservation and Hydrogenase Function in Methanogenic Archaea, in Particular the Genus Methanosarcina. United States: N. p., 2019. Web. doi:10.1128/MMBR.00020-19.
Mand, Thomas D., & Metcalf, William W. Energy Conservation and Hydrogenase Function in Methanogenic Archaea, in Particular the Genus Methanosarcina. United States. doi:10.1128/MMBR.00020-19.
Mand, Thomas D., and Metcalf, William W. Wed . "Energy Conservation and Hydrogenase Function in Methanogenic Archaea, in Particular the Genus Methanosarcina". United States. doi:10.1128/MMBR.00020-19.
@article{osti_1595766,
title = {Energy Conservation and Hydrogenase Function in Methanogenic Archaea, in Particular the Genus Methanosarcina},
author = {Mand, Thomas D. and Metcalf, William W.},
abstractNote = {The biological production of methane is vital to the global carbon cycle and accounts for ca. 74% of total methane emissions. The organisms that facilitate this process, methanogenic archaea, belong to a large and phylogenetically diverse group that thrives in a wide range of anaerobic environments. Two main subgroups exist within methanogenic archaea: those with and those without cytochromes. Although a variety of metabolisms exist within this group, the reduction of growth substrates to methane using electrons from molecular hydrogen is, in a phylogenetic sense, the most widespread methanogenic pathway. Methanogens without cytochromes typically generate methane by the reduction of CO2 with electrons derived from H2, formate, or secondary alcohols, generating a transmembrane ion gradient for ATP production via an Na+-translocating methyltransferase (Mtr). These organisms also conserve energy with a novel flavin-based electron bifurcation mechanism, wherein the endergonic reduction of ferredoxin is facilitated by the exergonic reduction of a disulfide terminal electron acceptor coupled to either H2 or formate oxidation. Methanogens that utilize cytochromes have a broader substrate range, and can convert acetate and methylated compounds to methane, in addition to the ability to reduce CO2. Cytochrome-containing methanogens are able to supplement the ion motive force generated by Mtr with an H+-translocating electron transport system. In both groups, enzymes known as hydrogenases, which reversibly interconvert protons and electrons to molecular hydrogen, play a central role in the methanogenic process. In this review, we discuss recent insight into methanogen metabolism and energy conservation mechanisms with a particular focus on the genus Methanosarcina.},
doi = {10.1128/MMBR.00020-19},
journal = {Microbiology and Molecular Biology Reviews},
number = 4,
volume = 83,
place = {United States},
year = {2019},
month = {9}
}

Journal Article:
Free Publicly Available Full Text
This content will become publicly available on September 18, 2020
Publisher's Version of Record

Save / Share:

Works referenced in this record:

Heterodisulfide reductase from methanogenic archaea: a new catalytic role for an iron-sulfur cluster
journal, January 2005

  • Hedderich, Reiner; Hamann, Nils; Bennati, Marina
  • Biological Chemistry, Vol. 386, Issue 10
  • DOI: 10.1515/BC.2005.112

Genetic, Biochemical, and Molecular Characterization of Methanosarcina barkeri Mutants Lacking Three Distinct Classes of Hydrogenase
journal, July 2018

  • Mand, Thomas D.; Kulkarni, Gargi; Metcalf, William W.
  • Journal of Bacteriology, Vol. 200, Issue 20
  • DOI: 10.1128/JB.00342-18

Hydrogen is a preferred intermediate in the energy-conserving electron transport chain of Methanosarcina barkeri
journal, September 2009

  • Kulkarni, Gargi; Kridelbaugh, Donna M.; Guss, Adam M.
  • Proceedings of the National Academy of Sciences, Vol. 106, Issue 37
  • DOI: 10.1073/pnas.0905914106

Biochemistry, evolution and physiological function of the Rnf complex, a novel ion-motive electron transport complex in prokaryotes
journal, November 2010

  • Biegel, Eva; Schmidt, Silke; González, José M.
  • Cellular and Molecular Life Sciences, Vol. 68, Issue 4
  • DOI: 10.1007/s00018-010-0555-8

Clostridium ultunense sp. nov., a Mesophilic Bacterium Oxidizing Acetate in Syntrophic Association with a Hydrogenotrophic Methanogenic Bacterium
journal, October 1996

  • Schnurer, A.; Schink, B.; Svensson, B. H.
  • International Journal of Systematic Bacteriology, Vol. 46, Issue 4
  • DOI: 10.1099/00207713-46-4-1145

Energy Conservation via Hydrogen Cycling in the Methanogenic Archaeon Methanosarcina barkeri
journal, July 2018


Heterodisulfide Reductase from Methanol-Grown Cells of Methanosarcina Barkeri is not a Flavoenzyme
journal, February 1997


Differences in Hydrogenase Gene Expression between Methanosarcina acetivorans and Methanosarcina barkeri
journal, February 2009

  • Guss, A. M.; Kulkarni, G.; Metcalf, W. W.
  • Journal of Bacteriology, Vol. 191, Issue 8
  • DOI: 10.1128/JB.00563-08

Methanofollis ethanolicus sp. nov., an ethanol-utilizing methanogen isolated from a lotus field
journal, April 2009

  • Imachi, H.; Sakai, S.; Nagai, H.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 59, Issue 4
  • DOI: 10.1099/ijs.0.003731-0

The F 420 H 2 :heterodisulfide oxidoreductase system from Methanosarcina species : 2-Hydroxyphenazine mediates electron transfer from F
journal, May 1998


Purification and Properties of the Heme- and Iron−Sulfur-Containing Heterodisulfide Reductase from Methanosarcina thermophila †
journal, July 1998

  • Simianu, Mihaela; Murakami, Eisuke; Brewer, John M.
  • Biochemistry, Vol. 37, Issue 28
  • DOI: 10.1021/bi9726483

Coupling of ATP synthesis and methane formation from methanol and molecular hydrogen in Methanosarcina barkeri
journal, May 1984


Purification and characterization of membrane-bound hydrogenase from Methanosarcina barkeri MS
journal, January 1994

  • Kemner, John M.; Zeikus, J. Gregory
  • Archives of Microbiology, Vol. 161, Issue 1
  • DOI: 10.1007/BF00248892

Methanomassiliicoccus luminyensis gen. nov., sp. nov., a methanogenic archaeon isolated from human faeces
journal, August 2012

  • Dridi, B.; Fardeau, M. -L.; Ollivier, B.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 62, Issue Pt 8
  • DOI: 10.1099/ijs.0.033712-0

Growth of acetotrophic, methane-producing bacteria in a pH auxostat
journal, July 1984

  • Sowers, Kevin R.; Nelson, Michael J.; Ferry, James G.
  • Current Microbiology, Vol. 11, Issue 4
  • DOI: 10.1007/BF01567165

Thermacetogenium phaeum gen. nov., sp. nov., a strictly anaerobic, thermophilic, syntrophic acetate-oxidizing bacterium
journal, July 2000

  • Hattori, S.; Kamagata, Y.; Hanada, S.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 50, Issue 4
  • DOI: 10.1099/00207713-50-4-1601

[FeFe]- and [NiFe]-hydrogenase diversity, mechanism, and maturation
journal, June 2015

  • Peters, John W.; Schut, Gerrit J.; Boyd, Eric S.
  • Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Vol. 1853, Issue 6
  • DOI: 10.1016/j.bbamcr.2014.11.021

The Membrane-Bound Electron Transport System of Methanosarcina Species
journal, February 2004


Classification of secondary alcohol-utilizing methanogens including a new thermophilic isolate
journal, September 1988

  • Widdel, F.; Rouvi�re, P. E.; Wolfe, R. S.
  • Archives of Microbiology, Vol. 150, Issue 5
  • DOI: 10.1007/BF00422290

Genetic analysis of the archaeon Methanosarcina barkeri Fusaro reveals a central role for Ech hydrogenase and ferredoxin in methanogenesis and carbon fixation
journal, April 2002

  • Meuer, J.; Kuettner, H. C.; Zhang, J. K.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 8
  • DOI: 10.1073/pnas.072615499

Bioenergetics of Archaea: ATP Synthesis under Harsh Environmental Conditions
journal, January 2005

  • Müller, V.; Lemker, T.; Lingl, A.
  • Journal of Molecular Microbiology and Biotechnology, Vol. 10, Issue 2-4
  • DOI: 10.1159/000091563

Growth Characteristics of Methanomassiliicoccus luminyensis and Expression of Methyltransferase Encoding Genes
journal, January 2017

  • Kröninger, Lena; Gottschling, Jacqueline; Deppenmeier, Uwe
  • Archaea, Vol. 2017
  • DOI: 10.1155/2017/2756573

Function of H 2 -forming methylenetetrahydromethanopterin dehydrogenase from Methanobacterium thermoautotrophicum in coenzyme F 420 reduction with H 2
journal, February 1998

  • Afting, C.; Hochheimer, A.; Thauer, R. K.
  • Archives of Microbiology, Vol. 169, Issue 3
  • DOI: 10.1007/s002030050562

Coupling of carbon monoxide oxidation to CO2 and H2 with the phosphorylation of ADP in acetate-grown Methanosarcina barkeri
journal, September 1986


Involvement of Ech hydrogenase in energy conservation of Methanosarcina mazei: Function of Ech in energy conservation
journal, July 2010


Methanophenazine: Structure, Total Synthesis, and Function of a New Cofactor from Methanogenic Archaea
journal, July 2000


Classification and phylogeny of hydrogenases
journal, August 2001


Protein complexing in a methanogen suggests electron bifurcation and electron delivery from formate to heterodisulfide reductase
journal, June 2010

  • Costa, K. C.; Wong, P. M.; Wang, T.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 24
  • DOI: 10.1073/pnas.1003653107

Chasing the elusive Euryarchaeota class WSA2: genomes reveal a uniquely fastidious methyl-reducing methanogen
journal, March 2016

  • Nobu, Masaru Konishi; Narihiro, Takashi; Kuroda, Kyohei
  • The ISME Journal, Vol. 10, Issue 10
  • DOI: 10.1038/ismej.2016.33

Methanosphaera stadtmaniae gen. nov., sp. nov.: a species that forms methane by reducing methanol with hydrogen
journal, March 1985

  • Miller, Terry L.; Wolin, Meyer J.
  • Archives of Microbiology, Vol. 141, Issue 2
  • DOI: 10.1007/BF00423270

Hydrogenotrophic methanogenesis in archaeal phylum Verstraetearchaeota reveals the shared ancestry of all methanogens
journal, February 2019

  • Berghuis, Bojk A.; Yu, Feiqiao Brian; Schulz, Frederik
  • Proceedings of the National Academy of Sciences, Vol. 116, Issue 11
  • DOI: 10.1073/pnas.1815631116

Non-aceticlastic methanogenesis from acetate: acetate oxidation by a thermophilic syntrophic coculture
journal, July 1984

  • Zinder, Stephen H.; Koch, Markus
  • Archives of Microbiology, Vol. 138, Issue 3
  • DOI: 10.1007/BF00402133

Occurrence, Classification, and Biological Function of Hydrogenases:  An Overview
journal, October 2007

  • Vignais, Paulette M.; Billoud, Bernard
  • Chemical Reviews, Vol. 107, Issue 10, p. 4206-4272
  • DOI: 10.1021/cr050196r

8-Hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum: 1. Purification and characterization
journal, July 1987

  • Fox, Judith A.; Livingston, David J.; Orme-Johnson, William H.
  • Biochemistry, Vol. 26, Issue 14
  • DOI: 10.1021/bi00388a007

The F 420 H 2 Dehydrogenase from Methanosarcina mazei Is a Redox-driven Proton Pump Closely Related to NADH Dehydrogenases
journal, April 2000

  • Bäumer, Sebastian; Ide, Tina; Jacobi, Carsten
  • Journal of Biological Chemistry, Vol. 275, Issue 24
  • DOI: 10.1074/jbc.M000650200

Methanococcus jannaschii sp. nov., an extremely thermophilic methanogen from a submarine hydrothermal vent
journal, December 1983

  • Jones, W. J.; Leigh, J. A.; Mayer, F.
  • Archives of Microbiology, Vol. 136, Issue 4
  • DOI: 10.1007/BF00425213

Energy-Converting [NiFe] Hydrogenases: More than Just H<sub>2</sub> Activation
journal, January 2005

  • Hedderich, Reiner; Forzi, Lucia
  • Journal of Molecular Microbiology and Biotechnology, Vol. 10, Issue 2-4
  • DOI: 10.1159/000091557

Molecular characterization of methanogenic N 5 -methyl-tetrahydromethanopterin: Coenzyme M methyltransferase
journal, September 2016

  • Upadhyay, Vikrant; Ceh, Katharina; Tumulka, Franz
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1858, Issue 9
  • DOI: 10.1016/j.bbamem.2016.06.011

Purification of the F420-reducing hydrogenase from Methanosarcina barkeri (strain Fusaro)
journal, September 1989


The transmembrane electrochemical gradient of Na+ as driving force for methanol oxidation in Methanosarcina barkeri
journal, March 1988


Genome Sequence of "Candidatus Methanomethylophilus alvus" Mx1201, a Methanogenic Archaeon from the Human Gut Belonging to a Seventh Order of Methanogens
journal, December 2012

  • Borrel, G.; Harris, H. M. B.; Tottey, W.
  • Journal of Bacteriology, Vol. 194, Issue 24
  • DOI: 10.1128/JB.01867-12

The Reaction Mechanism of Methyl-Coenzyme M Reductase: HOW AN ENZYME ENFORCES STRICT BINDING ORDER
journal, February 2015

  • Wongnate, Thanyaporn; Ragsdale, Stephen W.
  • Journal of Biological Chemistry, Vol. 290, Issue 15
  • DOI: 10.1074/jbc.M115.636761

Methanogenesis and the Wood–Ljungdahl Pathway: An Ancient, Versatile, and Fragile Association
journal, May 2016

  • Borrel, Guillaume; Adam, Panagiotis S.; Gribaldo, Simonetta
  • Genome Biology and Evolution, Vol. 8, Issue 6
  • DOI: 10.1093/gbe/evw114

Cell proliferation at 122 C and isotopically heavy CH4 production by a hyperthermophilic methanogen under high-pressure cultivation
journal, July 2008

  • Takai, K.; Nakamura, K.; Toki, T.
  • Proceedings of the National Academy of Sciences, Vol. 105, Issue 31
  • DOI: 10.1073/pnas.0712334105

Reduced coenzyme F420: heterodisulfide oxidoreductase, a proton- translocating redox system in methanogenic bacteria.
journal, December 1990

  • Deppenmeier, U.; Blaut, M.; Mahlmann, A.
  • Proceedings of the National Academy of Sciences, Vol. 87, Issue 23
  • DOI: 10.1073/pnas.87.23.9449

Electron transport during aceticlastic methanogenesis by Methanosarcina acetivorans involves a sodium-translocating Rnf complex
journal, November 2012

  • Schlegel, Katharina; Welte, Cornelia; Deppenmeier, Uwe
  • FEBS Journal, Vol. 279, Issue 24
  • DOI: 10.1111/febs.12031

Coupling of ferredoxin and heterodisulfide reduction via electron bifurcation in hydrogenotrophic methanogenic archaea
journal, January 2011

  • Kaster, A. -K.; Moll, J.; Parey, K.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 7
  • DOI: 10.1073/pnas.1016761108

Proton translocation coupled to the oxidation of carbon monoxide to CO2 and H2 in Methanosarcina barkeri
journal, February 1989


Energy-Converting [NiFe] Hydrogenases from Archaea and Extremophiles: Ancestors of Complex I
journal, February 2004


Methane from acetate.
journal, September 1992


Immunocytochemical localization of the coenzyme F420-reducing hydrogenase in Methanosarcina barkeri Fusaro.
journal, January 1991


Physiology, Biochemistry, and Applications of F 420 - and F o -Dependent Redox Reactions
journal, April 2016

  • Greening, Chris; Ahmed, F. Hafna; Mohamed, A. Elaaf
  • Microbiology and Molecular Biology Reviews, Vol. 80, Issue 2
  • DOI: 10.1128/MMBR.00070-15

Syntrophic Acetate-Oxidizing Microbes in Methanogenic Environments
journal, January 2008


De novo modeling of the F420-reducing [NiFe]-hydrogenase from a methanogenic archaeon by cryo-electron microscopy
journal, March 2013


An Escherichia coli hydrogenase-3-type hydrogenase in methanogenic archaea
journal, March 1998


Analysis of the vhoGAC and vhtGAC Operons from Methanosarcina mazei Strain Go1, Both Encoding a Membrane-bound Hydrogenase and a Cytochrome b
journal, January 1995


Different structure and expression of the operons encoding the membrane-bound hydrogenases fromMethanosarcina mazei Gö1
journal, November 1995


Methane metabolism in the archaeal phylum Bathyarchaeota revealed by genome-centric metagenomics
journal, October 2015


Enzymology of the fermentation of acetate to methane by Methanosarcina thermophila
journal, January 1997


Genetic Analysis of the Methanol- and Methylamine-Specific Methyltransferase 2 Genes of Methanosarcina acetivorans C2A
journal, March 2008

  • Bose, A.; Pritchett, M. A.; Metcalf, W. W.
  • Journal of Bacteriology, Vol. 190, Issue 11
  • DOI: 10.1128/JB.00117-08

Redox Potentials of Methanophenazine and CoB-S-S-CoM, Factors Involved in Electron Transport in Methanogenic Archaea
journal, March 2003


Thermotoga lettingae sp. nov., a novel thermophilic, methanol-degrading bacterium isolated from a thermophilic anaerobic reactor.
journal, July 2002

  • Stams, Alfons J. M.; Balk, Melike; Weijma, Jan
  • International Journal of Systematic and Evolutionary Microbiology, Vol. 52, Issue 4
  • DOI: 10.1099/00207713-52-4-1361

An evolving view of methane metabolism in the Archaea
journal, January 2019


Purification and characterization of coenzyme F420-dependent 5,10-methylenetetrahydromethanopterin dehydrogenase from Methanobacterium thermoautotrophicum strain ΔH
journal, January 1991

  • te Brömmelstroet, B. W.; Hensgens, Charles M. H.; Keltjens, Jan T.
  • Biochimica et Biophysica Acta (BBA) - General Subjects, Vol. 1073, Issue 1
  • DOI: 10.1016/0304-4165(91)90185-j

Methane Feedbacks to the Global Climate System in a Warmer World
journal, March 2018

  • Dean, Joshua F.; Middelburg, Jack J.; Röckmann, Thomas
  • Reviews of Geophysics, Vol. 56, Issue 1
  • DOI: 10.1002/2017RG000559

N5-methyl-tetrahydromethanopterin:coenzyme M methyltransferase of Methanosarcina strain Gö1 is an Na(+)-translocating membrane protein.
journal, December 1992


Electron transport in acetate-grown Methanosarcina acetivorans
journal, January 2011


Evolution of Na + and H + bioenergetics in methanogenic archaea
journal, February 2013

  • Schlegel, Katharina; Müller, Volker
  • Biochemical Society Transactions, Vol. 41, Issue 1
  • DOI: 10.1042/BST20120294

Genome-Scale Metabolic Reconstruction and Hypothesis Testing in the Methanogenic Archaeon Methanosarcina acetivorans C2A
journal, December 2011

  • Benedict, M. N.; Gonnerman, M. C.; Metcalf, W. W.
  • Journal of Bacteriology, Vol. 194, Issue 4
  • DOI: 10.1128/JB.06040-11

Promiscuous archaeal ATP synthase concurrently coupled to Na+ and H+ translocation
journal, January 2012

  • Schlegel, K.; Leone, V.; Faraldo-Gomez, J. D.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 3
  • DOI: 10.1073/pnas.1115796109

Sodium dependence of methane formation in methanogenic bacteria
journal, July 1982


Essential anaplerotic role for the energy-converting hydrogenase Eha in hydrogenotrophic methanogenesis
journal, August 2012

  • Lie, T. J.; Costa, K. C.; Lupa, B.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 38
  • DOI: 10.1073/pnas.1208779109

Proton-motive-force-driven formation of CO from CO2 and H2 in methanogenic bacteria
journal, October 1987


Thermodynamics of the Formylmethanofuran Dehydrogenase Reaction in Methanobacterium Thermoautotrophicum
journal, December 1994


Regulation of the synthesis of H 2 -forming methylenetetrahydromethanopterin dehydrogenase (Hmd) and of HmdII and HmdIII in Methanothermobacter marburgensis
journal, September 2000

  • Afting, Christina; Kremmer, Elisabeth; Brucker, Claudia
  • Archives of Microbiology, Vol. 174, Issue 4
  • DOI: 10.1007/s002030000197

MrpA Functions in Energy Conversion during Acetate-Dependent Growth of Methanosarcina acetivorans
journal, July 2013

  • Jasso-Chavez, R.; Apolinario, E. E.; Sowers, K. R.
  • Journal of Bacteriology, Vol. 195, Issue 17
  • DOI: 10.1128/JB.00581-13

Methanogenesis by Methanosarcina acetivorans involves two structurally and functionally distinct classes of heterodisulfide reductase
journal, February 2010


Bioenergetics of the formyl-methanofuran dehydrogenase and heterodisulfide reductase reactions in Methanothermobacter thermautotrophicus: Bioenergetics of H2-dependent methanogenic reactions
journal, December 2002


Purification and catalytic properties of Ech hydrogenase from Methanosarcina barkeri
journal, October 1999


My Kind of Biology
journal, October 1991


Membrane targeting and translocation of bacterial hydrogenases
journal, March 2000

  • Wu, Long-Fei; Chanal, Angélique; Rodrigue, Agnès
  • Archives of Microbiology, Vol. 173, Issue 5-6
  • DOI: 10.1007/s002030000144

Metabolic, Phylogenetic, and Ecological Diversity of the Methanogenic Archaea
journal, March 2008

  • Liu, Yuchen; Whitman, William B.
  • Annals of the New York Academy of Sciences, Vol. 1125, Issue 1
  • DOI: 10.1196/annals.1419.019

Energy conservation via electron bifurcating ferredoxin reduction and proton/Na+ translocating ferredoxin oxidation
journal, February 2013

  • Buckel, Wolfgang; Thauer, Rudolf K.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1827, Issue 2
  • DOI: 10.1016/j.bbabio.2012.07.002

Differences down-under: alcohol-fueled methanogenesis by archaea present in Australian macropodids
journal, March 2016

  • Hoedt, Emily C.; Cuív, Páraic Ó; Evans, Paul N.
  • The ISME Journal, Vol. 10, Issue 10
  • DOI: 10.1038/ismej.2016.41

Purification and properties of 5,10-methenyltetrahydromethanopterin cyclohydrolase from Methanosarcina barkeri.
journal, January 1990


The Wolfe cycle comes full circle
journal, September 2012


Bioenergetics and anaerobic respiratory chains of aceticlastic methanogens
journal, July 2014

  • Welte, Cornelia; Deppenmeier, Uwe
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1837, Issue 7
  • DOI: 10.1016/j.bbabio.2013.12.002

The Crystal Structure of [Fe]-Hydrogenase Reveals the Geometry of the Active Site
journal, July 2008


Methanogens: biochemical background and biotechnological applications
journal, January 2018


Methanogenic archaea: ecologically relevant differences in energy conservation
journal, June 2008

  • Thauer, Rudolf K.; Kaster, Anne-Kristin; Seedorf, Henning
  • Nature Reviews Microbiology, Vol. 6, Issue 8
  • DOI: 10.1038/nrmicro1931

Methylotrophic methanogenesis discovered in the archaeal phylum Verstraetearchaeota
journal, October 2016


Antigenic determinants of the membrane-bound hydrogenase in Alcaligenes eutrophus are exposed toward the periplasm.
journal, January 1995


Methanopyrus kandleri, gen. and sp. nov. represents a novel group of hyperthermophilic methanogens, growing at 110�C
journal, September 1991

  • Kurr, Margit; Huber, Robert; K�nig, Helmut
  • Archives of Microbiology, Vol. 156, Issue 4
  • DOI: 10.1007/BF00262992

Electron Transport in the Pathway of Acetate Conversion to Methane in the Marine Archaeon Methanosarcina acetivorans
journal, December 2005


Characterization of the RnfB and RnfG Subunits of the Rnf Complex from the Archaeon Methanosarcina acetivorans
journal, May 2014


N 5 , N 10 -Methylenetetrahydromethanopterin dehydrogenase from Methanobacterium thermoautotrophicum has hydrogenase activity
journal, February 1990


Purification and characterization of an 8-hydroxy-5-deazaflavin-reducing hydrogenase from the archaebacterium Methanococcus voltae
journal, December 1987


Rising atmospheric methane: 2007-2014 growth and isotopic shift: RISING METHANE 2007-2014
journal, September 2016

  • Nisbet, E. G.; Dlugokencky, E. J.; Manning, M. R.
  • Global Biogeochemical Cycles, Vol. 30, Issue 9
  • DOI: 10.1002/2016GB005406

Methanocella paludicola gen. nov., sp. nov., a methane-producing archaeon, the first isolate of the lineage 'Rice Cluster I', and proposal of the new archaeal order Methanocellales ord. nov.
journal, April 2008

  • Sakai, S.; Imachi, H.; Hanada, S.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 58, Issue 4
  • DOI: 10.1099/ijs.0.65571-0

Methane production from coal by a single methanogen
journal, October 2016


Sodium dependence of growth and methane formation in Methanobacterium thermoautotrophicum
journal, January 1981

  • Perski, Hans-Joachim; Moll, Johanna; Thauer, Rudolf K.
  • Archives of Microbiology, Vol. 130, Issue 4
  • DOI: 10.1007/BF00425947

Hydrogenases from Methanogenic Archaea, Nickel, a Novel Cofactor, and H 2 Storage
journal, June 2010


Physiological role of the F 420 -non-reducing hydrogenase (Mvh) from Methanothermobacter marburgensis
journal, September 2003

  • Stojanowic, Alexander; Mander, Gerd J.; Duin, Evert C.
  • Archives of Microbiology, Vol. 180, Issue 3
  • DOI: 10.1007/s00203-003-0577-9