Disentangling loosening from softening: insights into primary cell wall structure
Abstract
How cell wall elasticity, plasticity, and time-dependent extension (creep) relate to one another, to plant cell wall structure and to cell growth remain unsettled topics. To examine these issues without the complexities of living tissues, in this work we treated cell-free strips of onion epidermal walls with various enzymes and other agents to assess which polysaccharides bear mechanical forces in-plane and out-of-plane of the cell wall. This information is critical for integrating concepts of wall structure, wall material properties, tissue mechanics, and mechanisms of cell growth. With atomic force microscopy we also monitored real-time changes in the wall surface during treatments. Driselase, a potent cocktail of wall-degrading enzymes, removed cellulose microfibrils in superficial lamellae sequentially, layer-by-layer, and softened the wall (reduced its mechanical stiffness), yet did not induce wall loosening (creep). In contrast Cel12A, a bifunctional xyloglucanase/cellulase, induced creep with only subtle changes in wall appearance. Both Driselase and Cel12A increased the tensile compliance, but differently for elastic and plastic components. Homogalacturonan solubilization by pectate lyase and calcium chelation greatly increased the indentation compliance without changing tensile compliances. Acidic buffer induced rapid cell wall creep via endogenous α-expansins, with negligible effects on wall compliances. We conclude that these various wallmore »
- Authors:
-
- Pennsylvania State Univ., University Park, PA (United States). Dept. of Biology and Center for Lignocellulose Structure and Formation
- Lehigh Univ., Bethlehem, PA (United States). Dept. of Physics
- Publication Date:
- Research Org.:
- Energy Frontier Research Centers (EFRC) (United States). Center for Lignocellulose Structure and Formation (CLSF); Pennsylvania State Univ., University Park, PA (United States); Lehigh Univ., Bethlehem, PA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1595717
- Alternate Identifier(s):
- OSTI ID: 1567708
- Grant/Contract Number:
- SC0001090
- Resource Type:
- Accepted Manuscript
- Journal Name:
- The Plant Journal
- Additional Journal Information:
- Journal Volume: 100; Journal Issue: 6; Journal ID: ISSN 0960-7412
- Publisher:
- Society for Experimental Biology
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; biomechanics of primary cell walls; atomic force microscopy (AFM); Cel12A endoglucanase; driselase; cellulose; expansin; nano-indentation; onion (Allium cepa) epidermal cell wall; homogalacturonan; pectate lyase
Citation Formats
Zhang, Tian, Tang, Haosu, Vavylonis, Dimitrios, and Cosgrove, Daniel J. Disentangling loosening from softening: insights into primary cell wall structure. United States: N. p., 2019.
Web. doi:10.1111/tpj.14519.
Zhang, Tian, Tang, Haosu, Vavylonis, Dimitrios, & Cosgrove, Daniel J. Disentangling loosening from softening: insights into primary cell wall structure. United States. https://doi.org/10.1111/tpj.14519
Zhang, Tian, Tang, Haosu, Vavylonis, Dimitrios, and Cosgrove, Daniel J. Fri .
"Disentangling loosening from softening: insights into primary cell wall structure". United States. https://doi.org/10.1111/tpj.14519. https://www.osti.gov/servlets/purl/1595717.
@article{osti_1595717,
title = {Disentangling loosening from softening: insights into primary cell wall structure},
author = {Zhang, Tian and Tang, Haosu and Vavylonis, Dimitrios and Cosgrove, Daniel J.},
abstractNote = {How cell wall elasticity, plasticity, and time-dependent extension (creep) relate to one another, to plant cell wall structure and to cell growth remain unsettled topics. To examine these issues without the complexities of living tissues, in this work we treated cell-free strips of onion epidermal walls with various enzymes and other agents to assess which polysaccharides bear mechanical forces in-plane and out-of-plane of the cell wall. This information is critical for integrating concepts of wall structure, wall material properties, tissue mechanics, and mechanisms of cell growth. With atomic force microscopy we also monitored real-time changes in the wall surface during treatments. Driselase, a potent cocktail of wall-degrading enzymes, removed cellulose microfibrils in superficial lamellae sequentially, layer-by-layer, and softened the wall (reduced its mechanical stiffness), yet did not induce wall loosening (creep). In contrast Cel12A, a bifunctional xyloglucanase/cellulase, induced creep with only subtle changes in wall appearance. Both Driselase and Cel12A increased the tensile compliance, but differently for elastic and plastic components. Homogalacturonan solubilization by pectate lyase and calcium chelation greatly increased the indentation compliance without changing tensile compliances. Acidic buffer induced rapid cell wall creep via endogenous α-expansins, with negligible effects on wall compliances. We conclude that these various wall properties are not tightly coupled and thus reflect distinctive aspects of wall structure. Cross-lamellate networks of cellulose microfibrils influenced creep and tensile stiffness whereas homogalacturonan influenced indentation mechanics. This information is crucial for constructing realistic molecular models that define how wall mechanics and growth depend on primary cell wall structure.},
doi = {10.1111/tpj.14519},
journal = {The Plant Journal},
number = 6,
volume = 100,
place = {United States},
year = {Fri Aug 30 00:00:00 EDT 2019},
month = {Fri Aug 30 00:00:00 EDT 2019}
}
Web of Science
Works referenced in this record:
Solid-state NMR investigations of cellulose structure and interactions with matrix polysaccharides in plant primary cell walls
journal, September 2015
- Wang, Tuo; Hong, Mei
- Journal of Experimental Botany, Vol. 67, Issue 2
Rapid and reversible root growth inhibition by TIR1 auxin signalling
journal, June 2018
- Fendrych, Matyáš; Akhmanova, Maria; Merrin, Jack
- Nature Plants, Vol. 4, Issue 7
Biomechanical Characterization of Onion Epidermal Cell Walls
journal, January 2017
- Durachko, Daniel; Park, Yong Bum; Zhang, Tian
- BIO-PROTOCOL, Vol. 7, Issue 24
Salinity Stiffens the Epidermal Cell Walls of Salt-Stressed Maize Leaves: Is the Epidermis Growth-Restricting?
journal, March 2015
- Zörb, Christian; Mühling, Karl H.; Kutschera, Ulrich
- PLOS ONE, Vol. 10, Issue 3
Quantifying Hydrostatic Pressure in Plant Cells by Using Indentation with an Atomic Force Microscope
journal, May 2015
- Beauzamy, Léna; Derr, Julien; Boudaoud, Arezki
- Biophysical Journal, Vol. 108, Issue 10
Regulation of plant cell wall stiffness by mechanical stress: a mesoscale physical model
journal, September 2018
- Oliveri, Hadrien; Traas, Jan; Godin, Christophe
- Journal of Mathematical Biology, Vol. 78, Issue 3
Physical extensibility of maize coleoptile cell walls: apparent plastic extensibility is due to elastic hysteresis
journal, July 1992
- Hohl, M.; Schopfer, P.
- Planta, Vol. 187, Issue 4
Finite Element Modeling of Shape Changes in Plant Cells
journal, December 2017
- Bidhendi, Amir J.; Geitmann, Anja
- Plant Physiology, Vol. 176, Issue 1
Spatial organization of cellulose microfibrils and matrix polysaccharides in primary plant cell walls as imaged by multichannel atomic force microscopy
journal, January 2016
- Zhang, Tian; Zheng, Yunzhen; Cosgrove, Daniel J.
- The Plant Journal, Vol. 85, Issue 2
Growth of the plant cell wall
journal, November 2005
- Cosgrove, Daniel J.
- Nature Reviews Molecular Cell Biology, Vol. 6, Issue 11, p. 850-861
The Control of Growth Symmetry Breaking in the Arabidopsis Hypocotyl
journal, June 2015
- Peaucelle, Alexis; Wightman, Raymond; Höfte, Herman
- Current Biology, Vol. 25, Issue 13
Molecular dynamics simulation study of xyloglucan adsorption on cellulose surfaces: effects of surface hydrophobicity and side-chain variation
journal, September 2013
- Zhao, Zhen; Crespi, Vincent H.; Kubicki, James D.
- Cellulose, Vol. 21, Issue 2
Pectin Chemistry and Cellulose Crystallinity Govern Pavement Cell Morphogenesis in a Multi-Step Mechanism
journal, July 2019
- Altartouri, Bara; Bidhendi, Amir J.; Tani, Tomomi
- Plant Physiology, Vol. 181, Issue 1
The Instron technique as a measure of immediate-past wall extensibility
journal, May 1984
- Cleland, Robert E.
- Planta, Vol. 160, Issue 6
A Revised Architecture of Primary Cell Walls Based on Biomechanical Changes Induced by Substrate-Specific Endoglucanases
journal, February 2012
- Park, Yong Bum; Cosgrove, Daniel J.
- Plant Physiology, Vol. 158, Issue 4
Xyloglucan Deficiency Disrupts Microtubule Stability and Cellulose Biosynthesis in Arabidopsis, Altering Cell Growth and Morphogenesis
journal, November 2015
- Xiao, Chaowen; Zhang, Tian; Zheng, Yunzhen
- Plant Physiology, Vol. 170, Issue 1
Autolysis and extension of isolated walls from growing cucumber hypocotyls
journal, November 1994
- Cosgrove, Daniel J.; Durachko, Daniel M.
- Journal of Experimental Botany, Vol. 45, Issue Special_Issue
Cellular growth in plants requires regulation of cell wall biochemistry
journal, February 2017
- Chebli, Youssef; Geitmann, Anja
- Current Opinion in Cell Biology, Vol. 44
Shifting foundations: the mechanical cell wall and development
journal, February 2016
- Braybrook, Siobhan A.; Jönsson, Henrik
- Current Opinion in Plant Biology, Vol. 29
Impact of acidic pH on plant cell wall polysaccharide structure and dynamics: insights into the mechanism of acid growth in plants from solid-state NMR
journal, October 2018
- Phyo, Pyae; Gu, Ying; Hong, Mei
- Cellulose, Vol. 26, Issue 1
Polysaccharide Conformation and Cell Wall Function
journal, June 1979
- Preston, R. D.
- Annual Review of Plant Physiology, Vol. 30, Issue 1
Atomic force microscopy based nanoindentation study of onion abaxial epidermis walls in aqueous environment
journal, January 2015
- Xi, Xiaoning; Kim, Seong H.; Tittmann, Bernhard
- Journal of Applied Physics, Vol. 117, Issue 2
Subcellular and supracellular mechanical stress prescribes cytoskeleton behavior in Arabidopsis cotyledon pavement cells
journal, April 2014
- Sampathkumar, Arun; Krupinski, Pawel; Wightman, Raymond
- eLife, Vol. 3
Size, shape, and arrangement of native cellulose fibrils in maize cell walls
journal, December 2013
- Ding, Shi-You; Zhao, Shuai; Zeng, Yining
- Cellulose, Vol. 21, Issue 2
Segmentation and tracking of cytoskeletal filaments using open active contours
journal, October 2010
- Smith, Matthew B.; Li, Hongsheng; Shen, Tian
- Cytoskeleton, Vol. 67, Issue 11
Visualization of the nanoscale pattern of recently-deposited cellulose microfibrils and matrix materials in never-dried primary walls of the onion epidermis
journal, July 2013
- Zhang, Tian; Mahgsoudy-Louyeh, Sahar; Tittmann, Bernhard
- Cellulose, Vol. 21, Issue 2
Characterization of long-term extension of isolated cell walls from growing cucumber hypocotyls
journal, January 1989
- Cosgrove, Daniel J.
- Planta, Vol. 177, Issue 1
The relation between load and penetration in the axisymmetric boussinesq problem for a punch of arbitrary profile
journal, May 1965
- Sneddon, Ian N.
- International Journal of Engineering Science, Vol. 3, Issue 1
The Structure and Plastic Properties of the Cell Wall of Nitella in Relation to Extension Growth
journal, January 1966
- Probine, Mc; Barber, Nf
- Australian Journal of Biological Sciences, Vol. 19, Issue 3
Pectin-Induced Changes in Cell Wall Mechanics Underlie Organ Initiation in Arabidopsis
journal, October 2011
- Peaucelle, Alexis; Braybrook, Siobhan A.; Le Guillou, Laurent
- Current Biology, Vol. 21, Issue 20
Restoration of Mature Etiolated Cucumber Hypocotyl Cell Wall Susceptibility to Expansin by Pretreatment with Fungal Pectinases and EGTA in Vitro
journal, June 2008
- Zhao, Qingxin; Yuan, Sheng; Wang, Xin
- Plant Physiology, Vol. 147, Issue 4
Control of thickness of collenchyma cell walls by pectins
journal, May 1992
- Jarvis, M. C.
- Planta, Vol. 187, Issue 2
In vivo analysis of local wall stiffness at the shoot apical meristem in Arabidopsis using atomic force microscopy: Measuring wall stiffness in meristems with AFM
journal, July 2011
- Milani, Pascale; Gholamirad, Maryam; Traas, Jan
- The Plant Journal, Vol. 67, Issue 6
How Does Plant Cell Wall Nanoscale Architecture Correlate with Enzymatic Digestibility?
journal, November 2012
- Ding, S. -Y.; Liu, Y. -S.; Zeng, Y.
- Science, Vol. 338, Issue 6110
A Fungal Endoglucanase with Plant Cell Wall Extension Activity
journal, September 2001
- Yuan, Sheng; Wu, Yajun; Cosgrove, Daniel J.
- Plant Physiology, Vol. 127, Issue 1
The mechanical properties of plant cell walls soft material at the subcellular scale: the implications of water and of the intercellular boundaries
journal, July 2015
- Zamil, M. Shafayet; Yi, Hojae; Puri, Virendra M.
- Journal of Materials Science, Vol. 50, Issue 20
Continually emerging mechanistic complexity of the multi-enzyme cellulosome complex
journal, June 2017
- Smith, Steven P.; Bayer, Edward A.; Czjzek, Mirjam
- Current Opinion in Structural Biology, Vol. 44
Long-term acid-induced wall extension in an in-vitro system
journal, March 1987
- Cleland, R. E.; Cosgrove, D.; Tepfer, M.
- Planta, Vol. 170, Issue 3
Physicochemical Characteristics of Onion ( Allium cepa L.) Tissues
journal, November 2000
- Ng, Annie; Parker, Mary L.; Parr, Adrian J.
- Journal of Agricultural and Food Chemistry, Vol. 48, Issue 11
Nanoscale movements of cellulose microfibrils in primary cell walls
journal, April 2017
- Zhang, Tian; Vavylonis, Dimitrios; Durachko, Daniel M.
- Nature Plants, Vol. 3, Issue 5
Measuring the mechanics of morphogenesis
journal, February 2013
- Routier-Kierzkowska, Anne-Lise; Smith, Richard S.
- Current Opinion in Plant Biology, Vol. 16, Issue 1
Colorimetric Method for Determination of Sugars and Related Substances
journal, March 1956
- DuBois, Michel.; Gilles, K. A.; Hamilton, J. K.
- Analytical Chemistry, Vol. 28, Issue 3, p. 350-356
Mode of Action of Exo- and Endo-Type Cellulases from Irpex lacteus in the Hydrolysis of Cellulose with Different Crystallinities
journal, August 1993
- Hoshino, Eiichi; Sasaki, Yasushi; Okazaki, Mituo
- The Journal of Biochemistry, Vol. 114, Issue 2
Restricted access of proteins to mannan polysaccharides in intact plant cell walls: Masking of mannan polysaccharides
journal, August 2010
- Marcus, Susan E.; Blake, Anthony W.; Benians, Thomas A. S.
- The Plant Journal, Vol. 64, Issue 2
Electron Tomography of Cryo-Immobilized Plant Tissue: A Novel Approach to Studying 3D Macromolecular Architecture of Mature Plant Cell Walls In Situ
journal, September 2014
- Sarkar, Purbasha; Bosneaga, Elena; Yap, Edgar G.
- PLoS ONE, Vol. 9, Issue 9
A synthetic biology approach for evaluating the functional contribution of designer cellulosome components to deconstruction of cellulosic substrates
journal, January 2013
- Vazana, Yael; Barak, Yoav; Unger, Tamar
- Biotechnology for Biofuels, Vol. 6, Issue 1
Nanoscale structure, mechanics and growth of epidermal cell walls
journal, December 2018
- Cosgrove, Daniel J.
- Current Opinion in Plant Biology, Vol. 46
The Growing Outer Epidermal Wall: Design and Physiological Role of a Composite Structure
journal, February 2008
- Kutschera, U.
- Annals of Botany, Vol. 101, Issue 5
Dual-axis electron tomography: a new approach for investigating the spatial organization of wood cellulose microfibrils
journal, July 2006
- Xu, Ping; Donaldson, Lloyd A.; Gergely, Zachary R.
- Wood Science and Technology, Vol. 41, Issue 2
Cellular Force Microscopy for in Vivo Measurements of Plant Tissue Mechanics
journal, February 2012
- Routier-Kierzkowska, Anne-Lise; Weber, Alain; Kochova, Petra
- Plant Physiology, Vol. 158, Issue 4
Sensitivity-enhanced solid-state NMR detection of expansin's target in plant cell walls
journal, September 2013
- Wang, T.; Park, Y. B.; Caporini, M. A.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 41
Mechanochemical Polarization of Contiguous Cell Walls Shapes Plant Pavement Cells
journal, November 2017
- Majda, Mateusz; Grones, Peter; Sintorn, Ida-Maria
- Developmental Cell, Vol. 43, Issue 3
Changes in Cell Wall Biomechanical Properties in the Xyloglucan-Deficient xxt1/xxt2 Mutant of Arabidopsis
journal, November 2011
- Park, Yong Bum; Cosgrove, Daniel J.
- Plant Physiology, Vol. 158, Issue 1
Why plants make puzzle cells, and how their shape emerges
journal, February 2018
- Sapala, Aleksandra; Runions, Adam; Routier-Kierzkowska, Anne-Lise
- eLife, Vol. 7
Architecture-Based Multiscale Computational Modeling of Plant Cell Wall Mechanics to Examine the Hydrogen-Bonding Hypothesis of the Cell Wall Network Structure Model
journal, August 2012
- Yi, Hojae; Puri, Virendra M.
- Plant Physiology, Vol. 160, Issue 3
Extensibility of isolated cell walls: Measurement and changes during cell elongation
journal, January 1967
- Cleland, Robert
- Planta, Vol. 74, Issue 3
Multiscale stress-strain characterization of onion outer epidermal tissue in wet and dry states
journal, January 2015
- Kim, Keekyoung; Yi, Hojae; Zamil, M. Shafayet
- American Journal of Botany, Vol. 102, Issue 1
Shrinking the hammer: micromechanical approaches to morphogenesis
journal, July 2013
- Milani, Pascale; Braybrook, Siobhan A.; Boudaoud, Arezki
- Journal of Experimental Botany, Vol. 64, Issue 15
Plant expansins: diversity and interactions with plant cell walls
journal, June 2015
- Cosgrove, Daniel J.
- Current Opinion in Plant Biology, Vol. 25
Mechanical characterization of outer epidermal middle lamella of onion under tensile loading
journal, May 2014
- Zamil, M. Shafayet; Yi, Hojae; Puri, Virendra M.
- American Journal of Botany, Vol. 101, Issue 5
Patterning mechanisms of cytoskeletal and cell wall systems during leaf trichome morphogenesis
journal, March 2015
- Yanagisawa, Makoto; Desyatova, Anastasia S.; Belteton, Samuel A.
- Nature Plants, Vol. 1, Issue 3
Synergism between cucumber α-expansin, fungal endoglucanase and pectin lyase
journal, September 2010
- Wei, Wei; Yang, Chun; Luo, Jun
- Journal of Plant Physiology, Vol. 167, Issue 14
Differential Regulation of Cellulose Orientation at the Inner and Outer Face of Epidermal Cells in the Arabidopsis Hypocotyl
journal, July 2011
- Crowell, Elizabeth Faris; Timpano, Hélène; Desprez, Thierry
- The Plant Cell, Vol. 23, Issue 7
Cellulosomes: bacterial nanomachines for dismantling plant polysaccharides
journal, December 2016
- Artzi, Lior; Bayer, Edward A.; Moraïs, Sarah
- Nature Reviews Microbiology, Vol. 15, Issue 2
3D Electron Tomography of Pretreated Biomass Informs Atomic Modeling of Cellulose Microfibrils
journal, August 2013
- Ciesielski, Peter N.; Matthews, James F.; Tucker, Melvin P.
- ACS Nano, Vol. 7, Issue 9
On the role of stress anisotropy in the growth of stems
journal, August 2013
- Baskin, Tobias I.; Jensen, Oliver E.
- Journal of Experimental Botany, Vol. 64, Issue 15
TIR1/AFB-Aux/IAA auxin perception mediates rapid cell wall acidification and growth of Arabidopsis hypocotyls
journal, September 2016
- Fendrych, Matyáš; Leung, Jeffrey; Friml, Jiří
- eLife, Vol. 5
An oat coleoptile wall protein that induces wall extension in vitro and that is antigenically related to a similar protein from cucumber hypocotyls
journal, August 1993
- Li, Zhen-Chang; Durachko, DanielM.; Cosgrove, DanielJ.
- Planta, Vol. 191, Issue 3
Loosening of plant cell walls by expansins
journal, September 2000
- Cosgrove, Daniel J.
- Nature, Vol. 407, Issue 6802
Cellulose-Pectin Spatial Contacts Are Inherent to Never-Dried Arabidopsis Primary Cell Walls: Evidence from Solid-State Nuclear Magnetic Resonance
journal, June 2015
- Wang, Tuo; Park, Yong Bum; Cosgrove, Daniel J.
- Plant Physiology, Vol. 168, Issue 3
Preparation of Onion Epidermal Cell Walls for Imaging by Atomic Force Microscopy (AFM)
journal, January 2017
- Zhang, Tian; Cosgrove, Daniel
- BIO-PROTOCOL, Vol. 7, Issue 24
Plant cell wall extensibility: connecting plant cell growth with cell wall structure, mechanics, and the action of wall-modifying enzymes
journal, November 2015
- Cosgrove, Daniel J.
- Journal of Experimental Botany, Vol. 67, Issue 2
WallGen, Software to Construct Layered Cellulose-Hemicellulose Networks and Predict Their Small Deformation Mechanics
journal, December 2009
- Kha, Hung; Tuble, Sigrid C.; Kalyanasundaram, Shankar
- Plant Physiology, Vol. 152, Issue 2
Disrupting Two Arabidopsis thaliana Xylosyltransferase Genes Results in Plants Deficient in Xyloglucan, a Major Primary Cell Wall Component
journal, June 2008
- Cavalier, David M.; Lerouxel, Olivier; Neumetzler, Lutz
- The Plant Cell, Vol. 20, Issue 6
Effects of mechanical stretching on average orientation of cellulose and pectin in onion epidermis cell wall: A polarized FT-IR study
journal, May 2017
- Kafle, Kabindra; Park, Yong Bum; Lee, Christopher M.
- Cellulose, Vol. 24, Issue 8
Assessment of In Vitro Binding of Isolated Pectic Domains to Cellulose by Adsorption Isotherms, Electron Microscopy, and X-ray Diffraction Methods
journal, January 2007
- Zykwinska, Agata; Gaillard, Cédric; Buléon, Alain
- Biomacromolecules, Vol. 8, Issue 1
On the micro-indentation of plant cells in a tissue context
journal, February 2017
- Mosca, Gabriella; Sapala, Aleksandra; Strauss, Soeren
- Physical Biology, Vol. 14, Issue 1
Diffuse Growth of Plant Cell Walls
journal, November 2017
- Cosgrove, Daniel J.
- Plant Physiology, Vol. 176, Issue 1
Biomass accessibility analysis using electron tomography
journal, December 2015
- Hinkle, Jacob D.; Ciesielski, Peter N.; Gruchalla, Kenny
- Biotechnology for Biofuels, Vol. 8, Issue 1
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
journal, May 1976
- Bradford, Marion M.
- Analytical Biochemistry, Vol. 72, Issue 1-2
Growing cell walls show a gradient of elastic strain across their layers
journal, June 2018
- Lipowczan, Marcin; Borowska-Wykręt, Dorota; Natonik-Białoń, Sandra
- Journal of Experimental Botany, Vol. 69, Issue 18
Effects of Pectin Molecular Weight Changes on the Structure, Dynamics, and Polysaccharide Interactions of Primary Cell Walls of Arabidopsis thaliana : Insights from Solid-State NMR
journal, August 2017
- Phyo, Pyae; Wang, Tuo; Xiao, Chaowen
- Biomacromolecules, Vol. 18, Issue 9
Cell wall extension results in the coordinate separation of parallel microfibrils: evidence from scanning electron microscopy and atomic force microscopy: Creep and cell wall ultrastructure
journal, June 2005
- Marga, Francoise; Grandbois, Michel; Cosgrove, Daniel J.
- The Plant Journal, Vol. 43, Issue 2
Transforms and Operators for Directional Bioimage Analysis: A Survey
book, May 2016
- Püspöki, Zsuzsanna; Storath, Martin; Sage, Daniel
- Focus on Bio-Image Informatics
Mechanical Properties of Plant Cell Walls Probed by Relaxation Spectra
journal, November 2010
- Hansen, Steen Laugesen; Ray, Peter Martin; Karlsson, Anders Ola
- Plant Physiology, Vol. 155, Issue 1
An Automated Confocal Micro-Extensometer Enables in Vivo Quantification of Mechanical Properties with Cellular Resolution
journal, November 2017
- Robinson, Sarah; Huflejt, Michal; Barbier de Reuille, Pierre
- The Plant Cell, Vol. 29, Issue 12
In vivo extraction of Arabidopsis cell turgor pressure using nanoindentation in conjunction with finite element modeling
journal, December 2012
- Forouzesh, Elham; Goel, Ashwani; Mackenzie, Sally A.
- The Plant Journal, Vol. 73, Issue 3
Plant Cell Expansion: Regulation of Cell Wall Mechanical Properties
journal, June 1984
- Taiz, L.
- Annual Review of Plant Physiology, Vol. 35, Issue 1
Examination of biological hotspot hypothesis of primary cell wall using a computational cell wall network model
journal, February 2015
- Nili, Abdolmadjid; Yi, Hojae; Crespi, Vincent H.
- Cellulose, Vol. 22, Issue 2
Anisotropic growth is achieved through the additive mechanical effect of material anisotropy and elastic asymmetry
journal, September 2018
- Bou Daher, Firas; Chen, Yuanjie; Bozorg, Behruz
- eLife, Vol. 7
Measuring the mechanical properties of plant cells by combining micro-indentation with osmotic treatments
journal, April 2015
- Weber, Alain; Braybrook, Siobhan; Huflejt, Michal
- Journal of Experimental Botany, Vol. 66, Issue 11
A pyramid approach to subpixel registration based on intensity
journal, January 1998
- Thevenaz, P.; Ruttimann, U. E.; Unser, M.
- IEEE Transactions on Image Processing, Vol. 7, Issue 1
Gradients in Wall Mechanics and Polysaccharides along Growing Inflorescence Stems
journal, October 2017
- Phyo, Pyae; Wang, Tuo; Kiemle, Sarah N.
- Plant Physiology, Vol. 175, Issue 4
Re-constructing our models of cellulose and primary cell wall assembly
journal, December 2014
- Cosgrove, Daniel J.
- Current Opinion in Plant Biology, Vol. 22
The Mechanical Properties and Molecular Dynamics of Plant Cell Wall Polysaccharides Studied by Fourier-Transform Infrared Spectroscopy
journal, September 2000
- Wilson, Reginald H.; Smith, Andrew C.; Kačuráková, Marta
- Plant Physiology, Vol. 124, Issue 1
Xyloglucan in the primary cell wall: assessment by FESEM , selective enzyme digestions and nanogold affinity tags
journal, December 2017
- Zheng, Yunzhen; Wang, Xuan; Chen, Yuning
- The Plant Journal, Vol. 93, Issue 2
Segmentation and Tracking of Cytoskeletal Filaments Using Open Active Contours
journal, February 2011
- Smith, Matthew B.; Li, Hongsheng; Shen, Tian
- Biophysical Journal, Vol. 100, Issue 3
Probiotic properties of a phytase producing Pediococcus acidilactici strain SMVDUDB2 isolated from traditional fermented cheese product, Kalarei
journal, February 2020
- Bhagat, Deepali; Raina, Neelu; Kumar, Amit
- Scientific Reports, Vol. 10, Issue 1
Mechanochemical Polarization of Contiguous Cell Walls Shapes Plant Pavement Cells.
text, January 2017
- Majda, Mateusz; Grones, Peter; Sintorn, Ida-Maria
- Apollo - University of Cambridge Repository
On the micro-indentation of plant cells in a tissue context
text, January 2017
- Strauss, Soeren; Mosca, Gabriella; Smith, Richard S.
- IOP Publishing
Measuring the mechanics of morphogenesis
text, January 2013
- Smith, Richard S.; Routier-Kierzkowska, Anne-Lise
- Elsevier Science
Measuring the mechanical properties of plant cells by combining micro-indentation with osmotic treatments
text, January 2015
- Weber, Alain; Braybrook, Siobhan; Huflejt, Michal
- Oxford University Press
The Control of Growth Symmetry Breaking in the Arabidopsis Hypocotyl
journal, June 2015
- Peaucelle, Alexis; Wightman, Raymond; Höfte, Herman
- Current Biology, Vol. 25, Issue 13
Subcellular and supracellular mechanical stress prescribes cytoskeleton behavior in Arabidopsis cotyledon pavement cells.
text, January 2014
- Sampathkumar, Arun; Krupinski, Pawel; Wightman, Raymond
- Apollo - University of Cambridge Repository
Why plants make puzzle cells, and how their shape emerges
text, January 2018
- Sapala, Aleksandra; Runions, Adam; Routier-Kierzkowska, Anne-Lise
- eLife Sciences Publications Ltd.
Cellular Force Microscopy for in Vivo Measurements of Plant Tissue Mechanics
text, January 2012
- -L, Routier-Kierzkowska, A.; Weber, A.; Kochova, P.
- American Society of Plant Biologists
An automated confocal micro-extensometer enables in vivo quantification of mechanical properties with cellular resolution.
text, January 2017
- Robinson, Sarah Jane; Huflejt, Michal; Barbier De Reuille, Pierre
- Cold Spring Harbor Laboratory
Anisotropic growth is achieved through the additive mechanical effect of material anisotropy and elastic asymmetry.
text, January 2018
- Bou Daher, Firas; Chen, Yuanjie; Bozorg, Behruz
- Apollo - University of Cambridge Repository
An automated confocal micro-extensometer enables in vivo quantification of mechanical properties with cellular resolution.
text, January 2017
- Robinson, Sarah; Huflejt, Michal; Barbier De Reuille, Pierre
- American Society of Plant Biologists
Works referencing / citing this record:
Combining tensile testing and microscopy to address a diverse range of questions
journal, February 2020
- Robinson, Sarah; Durand‐Smet, Pauline
- Journal of Microscopy, Vol. 278, Issue 3
Pectin methylesterase selectively softens the onion epidermal wall yet reduces acid-induced creep
journal, February 2020
- Wang, Xuan; Wilson, Liza; Cosgrove, Daniel J.
- Journal of Experimental Botany, Vol. 71, Issue 9