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Title: Phase-contrast 3D tomography of HeLa cells grown in PLLA polymer electrospun scaffolds using synchrotron X-rays

Abstract

Advanced imaging is useful for understanding the three-dimensional (3D) growth of cells. X-ray tomography serves as a powerful noninvasive, nondestructive technique that can fulfill these purposes by providing information about cell growth within 3D platforms. There are a limited number of studies taking advantage of synchrotron X-rays, which provides a large field of view and suitable resolution to image cells within specific biomaterials. In this study, X-ray synchrotron radiation microtomography at Diamond Light Source and advanced image processing were used to investigate cellular infiltration of HeLa cells within poly L-lactide (PLLA) scaffolds. This study demonstrates that synchrotron X-rays using phase contrast is a useful method to understand the 3D growth of cells in PLLA electrospun scaffolds. Two different fiber diameter (2 and 4 µm) scaffolds with different pore sizes, grown over 2, 5 and 8 daysin vitro, were examined for infiltration and cell connectivity. After performing visualization by segmentation of the cells from the fibers, the results clearly show deeper cell growth and higher cellular interconnectivity in the 4 µm fiber diameter scaffold. Finally, this indicates the potential for using such 3D technology to study cell–scaffold interactions for future medical use.

Authors:
 [1];  [2];  [3];  [2];  [4];  [5];  [6];  [7];  [6]; ORCiD logo [8];  [9]
  1. Univ. College London (United Kingdom); Research Complex at Harwell Rutherford Appleton Lab. (United Kingdom)
  2. 3DMagination Ltd (United Kingdom)
  3. Univ. of Manchester (United Kingdom)
  4. The Univ. of Buckingham (United Kingdom)
  5. Univ. College London (United Kingdom); Research Complex at Harwell Rutherford Appleton Lab. (United Kingdom); Southern Univ. of Science and Technology (China). Dept. of Electrical and Electronic Engineering
  6. Diamond Light Source (United Kingdom)
  7. Univ. College London, London (United Kingdom)
  8. Univ. College London (United Kingdom); Research Complex at Harwell Rutherford Appleton Lab. (United Kingdom); Brookhaven National Lab. (BNL), Upton, NY (United States). Condensed Matter Physics and Materials Science Dept.
  9. Univ. College London (United Kingdom); Research Complex at Harwell Rutherford Appleton Lab. (United Kingdom); Aga Khan Univ., Karachi (Pakistan)
Publication Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1595696
Report Number(s):
BNL-213586-2020-JAAM
Journal ID: ISSN 1600-5775; JSYRES; TRN: US2101086
Grant/Contract Number:  
SC0012704
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Synchrotron Radiation (Online)
Additional Journal Information:
Journal Name: Journal of Synchrotron Radiation (Online); Journal Volume: 27; Journal Issue: 1; Journal ID: ISSN 1600-5775
Publisher:
International Union of Crystallography
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; 3D culture; electrospun polymer scaffold; X-ray CT; synchrotron; image analysis; Avizo

Citation Formats

Bhartiya, A., Madi, K., Disney, C. M., Courtois, L., Jupe, A., Zhang, F., Bodey, A. J., Lee, P., Rau, C., Robinson, I. K., and Yusuf, M. Phase-contrast 3D tomography of HeLa cells grown in PLLA polymer electrospun scaffolds using synchrotron X-rays. United States: N. p., 2020. Web. doi:10.1107/S1600577519015583.
Bhartiya, A., Madi, K., Disney, C. M., Courtois, L., Jupe, A., Zhang, F., Bodey, A. J., Lee, P., Rau, C., Robinson, I. K., & Yusuf, M. Phase-contrast 3D tomography of HeLa cells grown in PLLA polymer electrospun scaffolds using synchrotron X-rays. United States. doi:https://doi.org/10.1107/S1600577519015583
Bhartiya, A., Madi, K., Disney, C. M., Courtois, L., Jupe, A., Zhang, F., Bodey, A. J., Lee, P., Rau, C., Robinson, I. K., and Yusuf, M. Wed . "Phase-contrast 3D tomography of HeLa cells grown in PLLA polymer electrospun scaffolds using synchrotron X-rays". United States. doi:https://doi.org/10.1107/S1600577519015583. https://www.osti.gov/servlets/purl/1595696.
@article{osti_1595696,
title = {Phase-contrast 3D tomography of HeLa cells grown in PLLA polymer electrospun scaffolds using synchrotron X-rays},
author = {Bhartiya, A. and Madi, K. and Disney, C. M. and Courtois, L. and Jupe, A. and Zhang, F. and Bodey, A. J. and Lee, P. and Rau, C. and Robinson, I. K. and Yusuf, M.},
abstractNote = {Advanced imaging is useful for understanding the three-dimensional (3D) growth of cells. X-ray tomography serves as a powerful noninvasive, nondestructive technique that can fulfill these purposes by providing information about cell growth within 3D platforms. There are a limited number of studies taking advantage of synchrotron X-rays, which provides a large field of view and suitable resolution to image cells within specific biomaterials. In this study, X-ray synchrotron radiation microtomography at Diamond Light Source and advanced image processing were used to investigate cellular infiltration of HeLa cells within poly L-lactide (PLLA) scaffolds. This study demonstrates that synchrotron X-rays using phase contrast is a useful method to understand the 3D growth of cells in PLLA electrospun scaffolds. Two different fiber diameter (2 and 4 µm) scaffolds with different pore sizes, grown over 2, 5 and 8 daysin vitro, were examined for infiltration and cell connectivity. After performing visualization by segmentation of the cells from the fibers, the results clearly show deeper cell growth and higher cellular interconnectivity in the 4 µm fiber diameter scaffold. Finally, this indicates the potential for using such 3D technology to study cell–scaffold interactions for future medical use.},
doi = {10.1107/S1600577519015583},
journal = {Journal of Synchrotron Radiation (Online)},
number = 1,
volume = 27,
place = {United States},
year = {2020},
month = {1}
}

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