Phase-contrast 3D tomography of HeLa cells grown in PLLA polymer electrospun scaffolds using synchrotron X-rays

Bhartiya, A.; Madi, K.; Disney, C. M.; Courtois, L.; Jupe, A.; Zhang, F.; Bodey, A. J.; Lee, P.; Rau, C.; Robinson, I. K.; Yusuf, M.

doi:10.1107/S1600577519015583

Title: Phase-contrast 3D tomography of HeLa cells grown in PLLA polymer electrospun scaffolds using synchrotron X-rays

Journal Article · 01 January 2020 · Journal of Synchrotron Radiation (Online)

DOI: https://doi.org/10.1107/S1600577519015583 · OSTI ID:1595696

Bhartiya, A. ^[1]; Madi, K. ^[2]; Disney, C. M. ^[3]; Courtois, L. ^[2]; Jupe, A. ^[4]; Zhang, F. ^[5]; Bodey, A. J. ^[6]; Lee, P. ^[7]; Rau, C. ^[6];

^[8]; Yusuf, M. ^[9]

Univ. College London (United Kingdom); Research Complex at Harwell Rutherford Appleton Lab. (United Kingdom)
3DMagination Ltd (United Kingdom)
Univ. of Manchester (United Kingdom)
The Univ. of Buckingham (United Kingdom)
Univ. College London (United Kingdom); Research Complex at Harwell Rutherford Appleton Lab. (United Kingdom); Southern Univ. of Science and Technology (China). Dept. of Electrical and Electronic Engineering
Diamond Light Source (United Kingdom)
Univ. College London, London (United Kingdom)
Univ. College London (United Kingdom); Research Complex at Harwell Rutherford Appleton Lab. (United Kingdom); Brookhaven National Lab. (BNL), Upton, NY (United States). Condensed Matter Physics and Materials Science Dept.
Univ. College London (United Kingdom); Research Complex at Harwell Rutherford Appleton Lab. (United Kingdom); Aga Khan Univ., Karachi (Pakistan)

Advanced imaging is useful for understanding the three-dimensional (3D) growth of cells. X-ray tomography serves as a powerful noninvasive, nondestructive technique that can fulfill these purposes by providing information about cell growth within 3D platforms. There are a limited number of studies taking advantage of synchrotron X-rays, which provides a large field of view and suitable resolution to image cells within specific biomaterials. In this study, X-ray synchrotron radiation microtomography at Diamond Light Source and advanced image processing were used to investigate cellular infiltration of HeLa cells within poly L-lactide (PLLA) scaffolds. This study demonstrates that synchrotron X-rays using phase contrast is a useful method to understand the 3D growth of cells in PLLA electrospun scaffolds. Two different fiber diameter (2 and 4 µm) scaffolds with different pore sizes, grown over 2, 5 and 8 daysin vitro, were examined for infiltration and cell connectivity. After performing visualization by segmentation of the cells from the fibers, the results clearly show deeper cell growth and higher cellular interconnectivity in the 4 µm fiber diameter scaffold. Finally, this indicates the potential for using such 3D technology to study cell–scaffold interactions for future medical use.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0012704

OSTI ID:: 1595696

Report Number(s):: BNL-213586-2020-JAAM; JSYRES; TRN: US2101086

Journal Information:: Journal of Synchrotron Radiation (Online), Vol. 27, Issue 1; ISSN 1600-5775

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 6 works

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