This content will become publicly available on January 1, 2021
Phase-contrast 3D tomography of HeLa cells grown in PLLA polymer electrospun scaffolds using synchrotron X-rays
Abstract
Advanced imaging is useful for understanding the three-dimensional (3D) growth of cells. X-ray tomography serves as a powerful noninvasive, nondestructive technique that can fulfill these purposes by providing information about cell growth within 3D platforms. There are a limited number of studies taking advantage of synchrotron X-rays, which provides a large field of view and suitable resolution to image cells within specific biomaterials. In this study, X-ray synchrotron radiation microtomography at Diamond Light Source and advanced image processing were used to investigate cellular infiltration of HeLa cells within poly L-lactide (PLLA) scaffolds. This study demonstrates that synchrotron X-rays using phase contrast is a useful method to understand the 3D growth of cells in PLLA electrospun scaffolds. Two different fiber diameter (2 and 4 µm) scaffolds with different pore sizes, grown over 2, 5 and 8 daysin vitro, were examined for infiltration and cell connectivity. After performing visualization by segmentation of the cells from the fibers, the results clearly show deeper cell growth and higher cellular interconnectivity in the 4 µm fiber diameter scaffold. Finally, this indicates the potential for using such 3D technology to study cell–scaffold interactions for future medical use.
- Authors:
-
[8];
- Univ. College London (United Kingdom); Research Complex at Harwell Rutherford Appleton Lab. (United Kingdom)
- 3DMagination Ltd (United Kingdom)
- Univ. of Manchester (United Kingdom)
- The Univ. of Buckingham (United Kingdom)
- Univ. College London (United Kingdom); Research Complex at Harwell Rutherford Appleton Lab. (United Kingdom); Southern Univ. of Science and Technology (China). Dept. of Electrical and Electronic Engineering
- Diamond Light Source (United Kingdom)
- Univ. College London, London (United Kingdom)
- Univ. College London (United Kingdom); Research Complex at Harwell Rutherford Appleton Lab. (United Kingdom); Brookhaven National Lab. (BNL), Upton, NY (United States). Condensed Matter Physics and Materials Science Dept.
- Univ. College London (United Kingdom); Research Complex at Harwell Rutherford Appleton Lab. (United Kingdom); Aga Khan Univ., Karachi (Pakistan)
- Publication Date:
- Research Org.:
- Brookhaven National Lab. (BNL), Upton, NY (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
- OSTI Identifier:
- 1595696
- Report Number(s):
- [BNL-213586-2020-JAAM]
[Journal ID: ISSN 1600-5775; JSYRES]
- Grant/Contract Number:
- [SC0012704]
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Synchrotron Radiation (Online)
- Additional Journal Information:
- [Journal Name: Journal of Synchrotron Radiation (Online); Journal Volume: 27; Journal Issue: 1]; Journal ID: ISSN 1600-5775
- Publisher:
- International Union of Crystallography
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; 3D culture; electrospun polymer scaffold; X-ray CT; synchrotron; image analysis; Avizo
Citation Formats
Bhartiya, A., Madi, K., Disney, C. M., Courtois, L., Jupe, A., Zhang, F., Bodey, A. J., Lee, P., Rau, C., Robinson, I. K., and Yusuf, M. Phase-contrast 3D tomography of HeLa cells grown in PLLA polymer electrospun scaffolds using synchrotron X-rays. United States: N. p., 2020.
Web. doi:10.1107/S1600577519015583.
Bhartiya, A., Madi, K., Disney, C. M., Courtois, L., Jupe, A., Zhang, F., Bodey, A. J., Lee, P., Rau, C., Robinson, I. K., & Yusuf, M. Phase-contrast 3D tomography of HeLa cells grown in PLLA polymer electrospun scaffolds using synchrotron X-rays. United States. doi:10.1107/S1600577519015583.
Bhartiya, A., Madi, K., Disney, C. M., Courtois, L., Jupe, A., Zhang, F., Bodey, A. J., Lee, P., Rau, C., Robinson, I. K., and Yusuf, M. Wed .
"Phase-contrast 3D tomography of HeLa cells grown in PLLA polymer electrospun scaffolds using synchrotron X-rays". United States. doi:10.1107/S1600577519015583.
@article{osti_1595696,
title = {Phase-contrast 3D tomography of HeLa cells grown in PLLA polymer electrospun scaffolds using synchrotron X-rays},
author = {Bhartiya, A. and Madi, K. and Disney, C. M. and Courtois, L. and Jupe, A. and Zhang, F. and Bodey, A. J. and Lee, P. and Rau, C. and Robinson, I. K. and Yusuf, M.},
abstractNote = {Advanced imaging is useful for understanding the three-dimensional (3D) growth of cells. X-ray tomography serves as a powerful noninvasive, nondestructive technique that can fulfill these purposes by providing information about cell growth within 3D platforms. There are a limited number of studies taking advantage of synchrotron X-rays, which provides a large field of view and suitable resolution to image cells within specific biomaterials. In this study, X-ray synchrotron radiation microtomography at Diamond Light Source and advanced image processing were used to investigate cellular infiltration of HeLa cells within poly L-lactide (PLLA) scaffolds. This study demonstrates that synchrotron X-rays using phase contrast is a useful method to understand the 3D growth of cells in PLLA electrospun scaffolds. Two different fiber diameter (2 and 4 µm) scaffolds with different pore sizes, grown over 2, 5 and 8 daysin vitro, were examined for infiltration and cell connectivity. After performing visualization by segmentation of the cells from the fibers, the results clearly show deeper cell growth and higher cellular interconnectivity in the 4 µm fiber diameter scaffold. Finally, this indicates the potential for using such 3D technology to study cell–scaffold interactions for future medical use.},
doi = {10.1107/S1600577519015583},
journal = {Journal of Synchrotron Radiation (Online)},
number = [1],
volume = [27],
place = {United States},
year = {2020},
month = {1}
}
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Works referenced in this record:
Data Analysis WorkbeNch ( DAWN )
journal, April 2015
- Basham, Mark; Filik, Jacob; Wharmby, Michael T.
- Journal of Synchrotron Radiation, Vol. 22, Issue 3
A Review on Properties of Natural and Synthetic Based Electrospun Fibrous Materials for Bone Tissue Engineering
journal, August 2018
- Bhattarai, Deval; Aguilar, Ludwig; Park, Chan
- Membranes, Vol. 8, Issue 3
Microscopic Imaging and Spectroscopy with Scattered Light
journal, July 2010
- Boustany, Nada N.; Boppart, Stephen A.; Backman, Vadim
- Annual Review of Biomedical Engineering, Vol. 12, Issue 1
3D X-Ray Nanotomography of Cells Grown on Electrospun Scaffolds
journal, September 2016
- Bradley, Robert S.; Robinson, Ian K.; Yusuf, Mohammed
- Macromolecular Bioscience, Vol. 17, Issue 2
Correlative multiscale tomography of biological materials
journal, July 2016
- Bradley, Robert S.; Withers, Philip J.
- MRS Bulletin, Vol. 41, Issue 07
Scaffolds and cells for tissue regeneration: different scaffold pore sizes—different cell effects
journal, June 2015
- Bružauskaitė, Ieva; Bironaitė, Daiva; Bagdonas, Edvardas
- Cytotechnology, Vol. 68, Issue 3
A multi-functional scaffold for tissue regeneration: The need to engineer a tissue analogue
journal, December 2007
- Causa, Filippo; Netti, Paolo A.; Ambrosio, Luigi
- Biomaterials, Vol. 28, Issue 34
Practical limits of resolution in confocal and non-linear microscopy
journal, January 2003
- Cox, Guy; Sheppard, Colin J. R.
- Microscopy Research and Technique, Vol. 63, Issue 1
X-ray microcomputed tomography for the measurement of cell adhesionand proliferation in polymer scaffolds
journal, June 2009
- Dorsey, Shauna M.; Lin-Gibson, Sheng; Simon, Carl G.
- Biomaterials, Vol. 30, Issue 16
Engineering tumors with 3D scaffolds
journal, September 2007
- Fischbach, Claudia; Chen, Ruth; Matsumoto, Takuya
- Nature Methods, Vol. 4, Issue 10
Cell mechanics and the cytoskeleton
journal, January 2010
- Fletcher, Daniel A.; Mullins, R. Dyche
- Nature, Vol. 463, Issue 7280
Laboratory cryo x-ray microscopy for 3D cell imaging
journal, October 2017
- Fogelqvist, Emelie; Kördel, Mikael; Carannante, Valentina
- Scientific Reports, Vol. 7, Issue 1
Optical Spectroscopy and Imaging for the Noninvasive Evaluation of Engineered Tissues
journal, December 2008
- Georgakoudi, Irene; Rice, William L.; Hronik-Tupaj, Marie
- Tissue Engineering Part B: Reviews, Vol. 14, Issue 4
Phase-contrast microtomography: are the tracers necessary for stem cell tracking in infarcted hearts?
journal, August 2018
- Giuliani, Alessandra; Mencarelli, Mara; Frati, Caterina
- Biomedical Physics & Engineering Express, Vol. 4, Issue 5
A comparison study between electrospun polycaprolactone and piezoelectric poly(3-hydroxybutyrate-co-3-hydroxyvalerate) scaffolds for bone tissue engineering
journal, December 2017
- Gorodzha, Svetlana N.; Muslimov, Albert R.; Syromotina, Dina S.
- Colloids and Surfaces B: Biointerfaces, Vol. 160
Progress and Potential for Regenerative Medicine
journal, February 2007
- Gurtner, Geoffrey C.; Callaghan, Matthew J.; Longaker, Michael T.
- Annual Review of Medicine, Vol. 58, Issue 1
Porous scaffold design for tissue engineering
journal, July 2005
- Hollister, Scott J.
- Nature Materials, Vol. 4, Issue 7
Beyond HeLa cells
journal, November 2011
- Hyman, Anthony H.; Simons, Kai
- Nature, Vol. 480, Issue 7375
Effect of low-temperature plasma treatment of electrospun polycaprolactone fibrous scaffolds on calcium carbonate mineralisation
journal, January 2018
- Ivanova, Anna A.; Syromotina, Dina S.; Shkarina, Svetlana N.
- RSC Advances, Vol. 8, Issue 68
X-ray computed tomography
journal, June 2006
- Kalender, Willi A.
- Physics in Medicine and Biology, Vol. 51, Issue 13
Electrospinning for tissue engineering scaffolds
journal, April 2007
- Lannutti, J.; Reneker, D.; Ma, T.
- Materials Science and Engineering: C, Vol. 27, Issue 3
Developmental Engineering: A New Paradigm for the Design and Manufacturing of Cell-Based Products. Part I: From Three-Dimensional Cell Growth to Biomimetics of In Vivo Development
journal, December 2009
- Lenas, Petros; Moos, Malcolm; Luyten, Frank P.
- Tissue Engineering Part B: Reviews, Vol. 15, Issue 4
Electrospun nanofibrous structure: A novel scaffold for tissue engineering
journal, April 2002
- Li, Wan-Ju; Laurencin, Cato T.; Caterson, Edward J.
- Journal of Biomedical Materials Research, Vol. 60, Issue 4
Surface modification of poly(l-lactic acid) to improve its cytocompatibility via assembly of polyelectrolytes and gelatin
journal, March 2006
- Lin, Yuan; Wang, Luling; Zhang, Peibiao
- Acta Biomaterialia, Vol. 2, Issue 2
X-ray microtomography in biology
journal, February 2012
- Mizutani, Ryuta; Suzuki, Yoshio
- Micron, Vol. 43, Issue 2-3
The third dimension bridges the gap between cell culture and live tissue
journal, October 2007
- Pampaloni, Francesco; Reynaud, Emmanuel G.; Stelzer, Ernst H. K.
- Nature Reviews Molecular Cell Biology, Vol. 8, Issue 10, p. 839-845
Poly(lactide- co -glycolide) porous scaffolds for tissue engineering and regenerative medicine
journal, March 2012
- Pan, Zhen; Ding, Jiandong
- Interface Focus, Vol. 2, Issue 3
Experimental stations at I13 beamline at Diamond Light Source
journal, March 2013
- Pešić, Z. D.; Fanis, A. De; Wagner, U.
- Journal of Physics: Conference Series, Vol. 425, Issue 18
Laboratory x-ray micro-computed tomography: a user guideline for biological samples
journal, April 2017
- du Plessis, Anton; Broeckhoven, Chris; Guelpa, Anina
- GigaScience, Vol. 6, Issue 6
Coherent imaging at the Diamond beamline I13
journal, October 2011
- Rau, Christoph; Wagner, Ulrich; Pešić, Zoran
- physica status solidi (a), Vol. 208, Issue 11
Application of Synthetic Polymeric Scaffolds in Breast Cancer 3D Tissue Cultures and Animal Tumor Models
journal, January 2017
- Rijal, Girdhari; Bathula, Chandra; Li, Weimin
- International Journal of Biomaterials, Vol. 2017
A versatile 3D tissue matrix scaffold system for tumor modeling and drug screening
journal, September 2017
- Rijal, Girdhari; Li, Weimin
- Science Advances, Vol. 3, Issue 9
3D Cryo-Imaging: A Very High-Resolution View of the Whole Mouse
journal, March 2009
- Roy, Debashish; Steyer, Grant J.; Gargesha, Madhusudhana
- The Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology, Vol. 292, Issue 3
Computational modelling of cell spreading and tissue regeneration in porous scaffolds
journal, April 2007
- Sengers, Bram G.; Taylor, Mark; Please, Colin P.
- Biomaterials, Vol. 28, Issue 10
3D imaging of cells in scaffolds: direct labelling for micro CT
journal, June 2018
- Shepherd, David V.; Shepherd, Jennifer H.; Best, Serena M.
- Journal of Materials Science: Materials in Medicine, Vol. 29, Issue 6
3D biodegradable scaffolds of polycaprolactone with silicate-containing hydroxyapatite microparticles for bone tissue engineering: high-resolution tomography and in vitro study
journal, June 2018
- Shkarina, Svetlana; Shkarin, Roman; Weinhardt, Venera
- Scientific Reports, Vol. 8, Issue 1
3D imaging of cell interactions with electrospun PLGA nanofiber membranes for bone regeneration
journal, November 2015
- Stachewicz, Urszula; Qiao, Tuya; Rawlinson, Simon C. F.
- Acta Biomaterialia, Vol. 27
Electrospinning of nanofibers
journal, January 2005
- Subbiah, Thandavamoorthy; Bhat, G. S.; Tock, R. W.
- Journal of Applied Polymer Science, Vol. 96, Issue 2
Method to Analyze Three-Dimensional Cell Distribution and Infiltration in Degradable Scaffolds
journal, December 2008
- Thevenot, Paul; Nair, Ashwin; Dey, Jagannath
- Tissue Engineering Part C: Methods, Vol. 14, Issue 4
High resolution X-ray tomography – three-dimensional characterisation of cell–scaffold constructs for cartilage tissue engineering
journal, March 2014
- Zehbe, R.; Schmitt, V. H.; Kirkpatrick, C. J.
- Materials Science and Technology, Vol. 31, Issue 2