Combinatorial design of chemical-dependent protein switches for controlling intracellular electron transfer

Wu, Bingyan; Atkinson, Joshua T.; Kahanda, Dimithree; Bennett, George N.; Silberg, Jonathan J.

doi:10.1002/aic.16796

Title: Combinatorial design of chemical-dependent protein switches for controlling intracellular electron transfer

Journal Article · 10 September 2019 · AIChE Journal

DOI: https://doi.org/10.1002/aic.16796 · OSTI ID:1594060

Wu, Bingyan ^[1];

^[2]; Kahanda, Dimithree ^[2]; Bennett, George N. ^[2];

^[2]

Rice Univ., Houston, TX (United States); Rice University
Rice Univ., Houston, TX (United States)

One concern with controlling electron flow in cells is the lack of biomolecules that directly couple the sensing of environmental conditions to electron transfer efficiency. To overcome this protein component limitation, we randomly inserted the ligand binding domain (LBD) from the human estrogen receptor (ER) into a thermostable 2Fe-2S ferredoxin (Fd) from Mastigocladus laminosus and used a bacterial selection to identify Fd-LBD fusion proteins that support electron transfer from a Fd-NADP reductase (FNR) to a Fd-dependent sulfite reductase (SIR). Mapping LBD insertion sites onto structure revealed that Fd tolerates domain insertion adjacent to or within the tetracysteine motif that coordinates the 2Fe-2S metallocluster. With both classes of the fusion proteins, cellular ET was enhanced by the ER antagonist 4-hydroxytamoxifen, albeit to different extents. In addition, one of Fds arising from ER-LBD insertion within the tetracysteine motif was found to acquire an oxygen-tolerant 2Fe-2S cluster, suggesting that ET is regulated through post-translational ligand binding. Furthermore, these findings show that domain insertion can be used to create protein electron carriers whose ET is regulated by environmental conditions. These metalloprotein switches are expected to be useful for achieving fast regulation of ET in engineered metabolic pathways and between electroactive bacteria and conductive materials.

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Research Organization:: Rice Univ., Houston, TX (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); Office of Naval Research (ONR)

Grant/Contract Number:: SC0014462

OSTI ID:: 1594060

Journal Information:: AIChE Journal, Journal Name: AIChE Journal Journal Issue: 3 Vol. 66; ISSN 0001-1541

Publisher:: American Institute of Chemical EngineersCopyright Statement

Country of Publication:: United States

Language:: English

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36 MATERIALS SCIENCE
59 BASIC BIOLOGICAL SCIENCES
allostery
domain insertion
electron transfer
ferredoxin
growth selection
metalloprotein
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Title: Combinatorial design of chemical-dependent protein switches for controlling intracellular electron transfer

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