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Title: Natively oxidized amino acid residues in the spinach PS I-LHC I supercomplex

Abstract

Reactive oxygen species (ROS) production is an unavoidable byproduct of electron transport under aerobic conditions. Photosystem II (PS II), the cytochrome b6/ƒ complex and Photosystem I (PS I) are all demonstrated sources of ROS. It has been proposed that PS I produces substantial levels of a variety of ROS including O$$_{2}^{·-}$$, 1O2, H2O2 and, possibly, ∙OH, however, the site(s) of ROS production within PS I has been the subject of significant debate. We hypothesize that amino acid residues close to the sites of ROS generation will be more susceptible to oxidative modification than distant residues. In this study, we have identified oxidized amino acid residues in spinach PS I which was isolated from field-grown spinach. The modified residues were identified by high-resolution tandem mass spectrometry. As expected, many of the modified residues lie on the surface of the complex. However, a well-defined group of oxidized residues, both buried and surface-exposed, lead from the chl a’ of P700 to the surface of PS I. These residues (PsaB: 609F, 611E, 617M, 619W, 620L, and PsaF: 139L, 142A,143D) may identify a preferred route for ROS, probably 1O2, to egress the complex from the vicinity of P700. Additionally, two buried residues located in close proximity to A1B (PsaB:712H and 714S) were modified, which may be consistent with A1B being a source of O$$_{2}^{·-}$$. Surprisingly, no oxidatively modified residues were identified in close proximity to the 4Fe-FS clusters FX, FA or FB. These cofactors had been identified as a principal targets for ROS damage in the photosystem. Finally, a large number of residues located in the hydrophobic cores of Lhca1-Lhca4 are oxidatively modified. These appear to be the result of 1O2 production by the distal antennae for the photosystem.

Authors:
 [1];  [2];  [1]; ORCiD logo [1]
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  1. Louisiana State Univ., Baton Rouge, LA (United States)
  2. Univ. of Cincinnati, OH (United States)
Publication Date:
Research Org.:
Louisiana State Univ., Baton Rouge, LA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Chemical Sciences, Geosciences & Biosciences Division
OSTI Identifier:
1593960
Grant/Contract Number:  
FG02-98ER20310
Resource Type:
Accepted Manuscript
Journal Name:
Photosynthesis Research
Additional Journal Information:
Journal Volume: 143; Journal Issue: 3; Journal ID: ISSN 0166-8595
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Photosystem I; tandem mass spectrometry; Reactive Oxygen Species (ROS); spinach

Citation Formats

Kale, Ravindra, Sallans, Larry, Frankel, Laurie K., and Bricker, Terry M. Natively oxidized amino acid residues in the spinach PS I-LHC I supercomplex. United States: N. p., 2020. Web. doi:10.1007/s11120-019-00698-7.
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Kale, Ravindra, Sallans, Larry, Frankel, Laurie K., & Bricker, Terry M. Natively oxidized amino acid residues in the spinach PS I-LHC I supercomplex. United States. doi:10.1007/s11120-019-00698-7.
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Kale, Ravindra, Sallans, Larry, Frankel, Laurie K., and Bricker, Terry M. Wed . "Natively oxidized amino acid residues in the spinach PS I-LHC I supercomplex". United States. doi:10.1007/s11120-019-00698-7. https://www.osti.gov/servlets/purl/1593960.
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@article{osti_1593960,
title = {Natively oxidized amino acid residues in the spinach PS I-LHC I supercomplex},
author = {Kale, Ravindra and Sallans, Larry and Frankel, Laurie K. and Bricker, Terry M.},
abstractNote = {Reactive oxygen species (ROS) production is an unavoidable byproduct of electron transport under aerobic conditions. Photosystem II (PS II), the cytochrome b6/ƒ complex and Photosystem I (PS I) are all demonstrated sources of ROS. It has been proposed that PS I produces substantial levels of a variety of ROS including O$_{2}^{·-}$, 1O2, H2O2 and, possibly, ∙OH, however, the site(s) of ROS production within PS I has been the subject of significant debate. We hypothesize that amino acid residues close to the sites of ROS generation will be more susceptible to oxidative modification than distant residues. In this study, we have identified oxidized amino acid residues in spinach PS I which was isolated from field-grown spinach. The modified residues were identified by high-resolution tandem mass spectrometry. As expected, many of the modified residues lie on the surface of the complex. However, a well-defined group of oxidized residues, both buried and surface-exposed, lead from the chl a’ of P700 to the surface of PS I. These residues (PsaB: 609F, 611E, 617M, 619W, 620L, and PsaF: 139L, 142A,143D) may identify a preferred route for ROS, probably 1O2, to egress the complex from the vicinity of P700. Additionally, two buried residues located in close proximity to A1B (PsaB:712H and 714S) were modified, which may be consistent with A1B being a source of O$_{2}^{·-}$. Surprisingly, no oxidatively modified residues were identified in close proximity to the 4Fe-FS clusters FX, FA or FB. These cofactors had been identified as a principal targets for ROS damage in the photosystem. Finally, a large number of residues located in the hydrophobic cores of Lhca1-Lhca4 are oxidatively modified. These appear to be the result of 1O2 production by the distal antennae for the photosystem.},
doi = {10.1007/s11120-019-00698-7},
journal = {Photosynthesis Research},
number = 3,
volume = 143,
place = {United States},
year = {2020},
month = {1}
}
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