WHI-2 Regulates Intercellular Communication via a MAP Kinase Signaling Complex

Gonçalves, A. Pedro; Chow, Karen M.; Cea-Sánchez, Sara; Glass, N. Louise

doi:10.3389/fmicb.2019.03162

Title: WHI-2 Regulates Intercellular Communication via a MAP Kinase Signaling Complex

Journal Article · 22 January 2020 · Frontiers in Microbiology

DOI: https://doi.org/10.3389/fmicb.2019.03162 · OSTI ID:1593162

Gonçalves, A. Pedro ^[1]; Chow, Karen M. ^[2]; Cea-Sánchez, Sara ^[3]; Glass, N. Louise ^[4]

Univ. of California, Berkeley, CA (United States); Academia Sinica ,Taipei (Taiwan)
Univ. of California, Berkeley, CA (United States)
Univ. de Sevilla (Spain)
Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

The formation of the fungal mycelial network is facilitated by somatic cell fusion of germinating asexual spores (or germlings). Neurospora crassa germlings in close proximity display chemotropic growth that is dependent upon an intracellular network of mitogen-activated protein kinase (MAPK) signaling cascades. Approximately 80 genes involved in intercellular communication and fusion have been identified, including three mutants with similar morphological phenotypes: Δwhi-2, Δcsp-6, and Δamph-1. Here we show that WHI-2 localizes to the cell periphery and regulates endocytosis, mitochondrial organization, sporulation, and cell fusion. WHI-2 was required to transduce signals through a conserved MAPK pathway (NRC-1/MEK-2/MAK-2) and target transcription factors (PP-1/ADV-1). The amph-1 locus encodes a Bin/Amphiphysin/Rvs domain-containing protein and mis-expression of whi-2 compensated for the cell fusion and endocytosis deficiencies of a Δamph-1 mutant. The csp-6 locus encodes a haloacid dehalogenase phosphatase whose activity was essential for cell fusion. Although fusion-deficient with themselves, cells that lacked whi-2, csp-6, or amph-1 showed a low frequency of chemotropic interactions with wild type cells. We hypothesize that WHI-2 could be important for signal perception during chemotropic interactions via a role in endocytosis.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC); National Science Foundation (NSF)

Grant/Contract Number:: AC02-05CH11231; MCB1412411

OSTI ID:: 1593162

Alternate ID(s):: OSTI ID: 1604661

Journal Information:: Frontiers in Microbiology, Vol. 10; ISSN 1664-302X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 3 works

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59 BASIC BIOLOGICAL SCIENCES
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