Engineering of Saccharomyces cerevisiae for efficient fermentation of cellulose
Abstract
ABSTRACT Conversion of lignocellulosic biomass to biofuels using microbial fermentation is an attractive option to substitute petroleum-based production economically and sustainably. The substantial efforts to design yeast strains for biomass hydrolysis have led to industrially applicable biological routes. Saccharomyces cerevisiae is a robust microbial platform widely used in biofuel production, based on its amenability to systems and synthetic biology tools. The critical challenges for the efficient microbial conversion of lignocellulosic biomass by engineered S. cerevisiae include heterologous expression of cellulolytic enzymes, co-fermentation of hexose and pentose sugars, and robustness against various stresses. Scientists developed many engineering strategies for cellulolytic S. cerevisiae strains, bringing the application of consolidated bioprocess at an industrial scale. Recent advances in the development and implementation of engineered yeast strains capable of assimilating lignocellulose will be reviewed.
- Authors:
-
[2]
- Renewable and Sustainable Energy Institute (RASEI), University of Colorado Boulder, 4001 Discovery Dr., CO 80303, USA
- Department of Food Science and Human Nutrition, 905 S. Goodwin Ave., IL 61801, USA, 1105 Carl R. Woese Institute for Genomic Biology, 1206 W. Gregory Dr. Urbana, IL 61801. USA, DOE Center for Advanced Bioenergy and Bioproducts Innovation, University of Illinois at Urbana-Champaign, 1206 W. Gregory Dr. Urbana, IL 61801, USA
- Publication Date:
- Research Org.:
- Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1592780
- Alternate Identifier(s):
- OSTI ID: 1595946
- Grant/Contract Number:
- SC0018420
- Resource Type:
- Published Article
- Journal Name:
- FEMS Yeast Research
- Additional Journal Information:
- Journal Name: FEMS Yeast Research Journal Volume: 20 Journal Issue: 1; Journal ID: ISSN 1567-1356
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Saccharomyces cerevisiae; lignocellulosic biomass; biofuel
Citation Formats
Oh, Eun Joong, and Jin, Yong-Su. Engineering of Saccharomyces cerevisiae for efficient fermentation of cellulose. United Kingdom: N. p., 2020.
Web. doi:10.1093/femsyr/foz089.
Oh, Eun Joong, & Jin, Yong-Su. Engineering of Saccharomyces cerevisiae for efficient fermentation of cellulose. United Kingdom. https://doi.org/10.1093/femsyr/foz089
Oh, Eun Joong, and Jin, Yong-Su. Thu .
"Engineering of Saccharomyces cerevisiae for efficient fermentation of cellulose". United Kingdom. https://doi.org/10.1093/femsyr/foz089.
@article{osti_1592780,
title = {Engineering of Saccharomyces cerevisiae for efficient fermentation of cellulose},
author = {Oh, Eun Joong and Jin, Yong-Su},
abstractNote = {ABSTRACT Conversion of lignocellulosic biomass to biofuels using microbial fermentation is an attractive option to substitute petroleum-based production economically and sustainably. The substantial efforts to design yeast strains for biomass hydrolysis have led to industrially applicable biological routes. Saccharomyces cerevisiae is a robust microbial platform widely used in biofuel production, based on its amenability to systems and synthetic biology tools. The critical challenges for the efficient microbial conversion of lignocellulosic biomass by engineered S. cerevisiae include heterologous expression of cellulolytic enzymes, co-fermentation of hexose and pentose sugars, and robustness against various stresses. Scientists developed many engineering strategies for cellulolytic S. cerevisiae strains, bringing the application of consolidated bioprocess at an industrial scale. Recent advances in the development and implementation of engineered yeast strains capable of assimilating lignocellulose will be reviewed.},
doi = {10.1093/femsyr/foz089},
journal = {FEMS Yeast Research},
number = 1,
volume = 20,
place = {United Kingdom},
year = {2020},
month = {1}
}
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Works referenced in this record:
Cellodextrin Transport in Yeast for Improved Biofuel Production
journal, September 2010
- Galazka, J. M.; Tian, C.; Beeson, W. T.
- Science, Vol. 330, Issue 6000
Proximity Effect among Cellulose-Degrading Enzymes Displayed on the Saccharomyces cerevisiae Cell Surface
journal, October 2014
- Bae, Jungu; Kuroda, Kouichi; Ueda, Mitsuyoshi
- Applied and Environmental Microbiology, Vol. 81, Issue 1
The Cellulosomes: Multienzyme Machines for Degradation of Plant Cell Wall Polysaccharides
journal, October 2004
- Bayer, Edward A.; Belaich, Jean-Pierre; Shoham, Yuval
- Annual Review of Microbiology, Vol. 58, Issue 1
Efficient yeast cell-surface display of exo- and endo-cellulase using the SED1 anchoring region and its original promoter
journal, January 2014
- Inokuma, Kentaro; Hasunuma, Tomohisa; Kondo, Akihiko
- Biotechnology for Biofuels, Vol. 7, Issue 1
Agriculture: Beyond food versus fuel
journal, June 2011
- Graham-Rowe, Duncan
- Nature, Vol. 474, Issue 7352
Improvement of acetic acid tolerance of Saccharomyces cerevisiae using a zinc-finger-based artificial transcription factor and identification of novel genes involved in acetic acid tolerance
journal, January 2015
- Ma, Cui; Wei, Xiaowen; Sun, Cuihuan
- Applied Microbiology and Biotechnology, Vol. 99, Issue 5
Increased tolerance and conversion of inhibitors in lignocellulosic hydrolysates by Saccharomyces cerevisiae
journal, January 2007
- Almeida, Joao; Modig, Tobias; Petersson, Anneli
- Journal of Chemical Technology & Biotechnology, Vol. 82, Issue 4, p. 340-349
Simultaneously improving xylose fermentation and tolerance to lignocellulosic inhibitors through evolutionary engineering of recombinant Saccharomyces cerevisiae harbouring xylose isomerase
journal, January 2014
- Smith, Justin; van Rensburg, Eugéne; Görgens, Johann F.
- BMC Biotechnology, Vol. 14, Issue 1
Evaluation of Ethanol Production Activity by Engineered Saccharomyces cerevisiae Fermenting Cellobiose through the Phosphorolytic Pathway in Simultaneous Saccharification and Fermentation of Cellulose
journal, September 2017
- Lee, Won-Heong; Jin, Yong-Su
- Journal of Microbiology and Biotechnology, Vol. 27, Issue 9
Endowing non-cellulolytic microorganisms with cellulolytic activity aiming for consolidated bioprocessing
journal, November 2013
- Yamada, Ryosuke; Hasunuma, Tomohisa; Kondo, Akihiko
- Biotechnology Advances, Vol. 31, Issue 6
Fungal β-glucosidase expression in Saccharomyces cerevisiae
journal, June 2012
- Njokweni, A. P.; Rose, S. H.; van Zyl, W. H.
- Journal of Industrial Microbiology & Biotechnology, Vol. 39, Issue 10
Promiscuous activities of heterologous enzymes lead to unintended metabolic rerouting in Saccharomyces cerevisiae engineered to assimilate various sugars from renewable biomass
journal, May 2018
- Yun, Eun Ju; Oh, Eun Joong; Liu, Jing-Jing
- Biotechnology for Biofuels, Vol. 11, Issue 1
Pretreatment of lignocellulose: Formation of inhibitory by-products and strategies for minimizing their effects
journal, January 2016
- Jönsson, Leif J.; Martín, Carlos
- Bioresource Technology, Vol. 199
Yeast cell-surface display?applications of molecular display
journal, March 2004
- Kondo, A.; Ueda, M.
- Applied Microbiology and Biotechnology, Vol. 64, Issue 1
Enzymatic conversion of lignocellulose into fermentable sugars: challenges and opportunities
journal, October 2007
- Jørgensen, Henning; Kristensen, Jan Bach; Felby, Claus
- Biofuels, Bioproducts and Biorefining, Vol. 1, Issue 2
Cell wall structure suitable for surface display of proteins in Saccharomyces cerevisiae : Cell wall structure suitable for surface display of proteins
journal, January 2014
- Matsuoka, Hiroyuki; Hashimoto, Kazuya; Saijo, Aki
- Yeast, Vol. 31, Issue 2
Enhanced cellobiose fermentation by engineered Saccharomyces cerevisiae expressing a mutant cellodextrin facilitator and cellobiose phosphorylase
journal, June 2018
- Kim, Heejin; Oh, Eun Joong; Lane, Stephan Thomas
- Journal of Biotechnology, Vol. 275
Transcriptional profiling reveals molecular basis and novel genetic targets for improved resistance to multiple fermentation inhibitors in Saccharomyces cerevisiae
journal, January 2016
- Chen, Yingying; Sheng, Jiayuan; Jiang, Tao
- Biotechnology for Biofuels, Vol. 9, Issue 1
Simultaneous Utilization of Cellobiose, Xylose, and Acetic Acid from Lignocellulosic Biomass for Biofuel Production by an Engineered Yeast Platform
journal, November 2014
- Wei, Na; Oh, Eun Joong; Million, Gyver
- ACS Synthetic Biology, Vol. 4, Issue 6
Simultaneous co-fermentation of mixed sugars: a promising strategy for producing cellulosic ethanol
journal, May 2012
- Kim, Soo Rin; Ha, Suk-Jin; Wei, Na
- Trends in Biotechnology, Vol. 30, Issue 5
Comparative kinetic analysis of two fungal β-glucosidases
journal, January 2010
- Chauve, Marie; Mathis, Hugues; Huc, Delphine
- Biotechnology for Biofuels, Vol. 3, Issue 1
Synergistic Saccharification, and Direct Fermentation to Ethanol, of Amorphous Cellulose by Use of an Engineered Yeast Strain Codisplaying Three Types of Cellulolytic Enzyme
journal, February 2004
- Fujita, Y.; Ito, J.; Ueda, M.
- Applied and Environmental Microbiology, Vol. 70, Issue 2, p. 1207-1212
Consolidated bioprocessing of cellulosic biomass: an update
journal, October 2005
- Lynd, Lee R.; van Zyl, Willem H.; McBride, John E.
- Current Opinion in Biotechnology, Vol. 16, Issue 5, p. 577-583
Plasmid stability in recombinant Saccharomyces cerevisiae
journal, January 1996
- Zhang, Z.; Moo-Young, M.; Chisti, Y.
- Biotechnology Advances, Vol. 14, Issue 4
Direct and Efficient Production of Ethanol from Cellulosic Material with a Yeast Strain Displaying Cellulolytic Enzymes
journal, October 2002
- Fujita, Y.; Takahashi, S.; Ueda, M.
- Applied and Environmental Microbiology, Vol. 68, Issue 10
Development of yeast cell factories for consolidated bioprocessing of lignocellulose to bioethanol through cell surface engineering
journal, November 2012
- Hasunuma, Tomohisa; Kondo, Akihiko
- Biotechnology Advances, Vol. 30, Issue 6
Co-expression of a cellobiose phosphorylase and lactose permease enables intracellular cellobiose utilisation by Saccharomyces cerevisiae
journal, February 2011
- Sadie, Christa J.; Rose, Shaunita H.; den Haan, Riaan
- Applied Microbiology and Biotechnology, Vol. 90, Issue 4
Functional Display of Complex Cellulosomes on the Yeast Surface via Adaptive Assembly
journal, July 2012
- Tsai, Shen-Long; DaSilva, Nancy A.; Chen, Wilfred
- ACS Synthetic Biology, Vol. 2, Issue 1
Advanced Technology Paths to Global Climate Stability: Energy for a Greenhouse Planet
journal, November 2002
- Hoffert, M. I.; Caldeira, K.; Benford, G.
- Science, Vol. 298, Issue 5595
Combined cell-surface display- and secretion-based strategies for production of cellulosic ethanol with Saccharomyces cerevisiae
journal, September 2015
- Liu, Zhuo; Inokuma, Kentaro; Ho, Shih-Hsin
- Biotechnology for Biofuels, Vol. 8, Issue 1
Functional Assembly of Minicellulosomes on the Saccharomyces cerevisiae Cell Surface for Cellulose Hydrolysis and Ethanol Production
journal, August 2009
- Tsai, S. -L.; Oh, J.; Singh, S.
- Applied and Environmental Microbiology, Vol. 75, Issue 19
Simultaneous saccharification and fermentation by engineered Saccharomyces cerevisiae without supplementing extracellular β-glucosidase
journal, September 2013
- Lee, Won-Heong; Nan, Hong; Kim, Hyo Jin
- Journal of Biotechnology, Vol. 167, Issue 3
A new laboratory evolution approach to select for constitutive acetic acid tolerance in Saccharomyces cerevisiae and identification of causal mutations
journal, August 2016
- González-Ramos, Daniel; Gorter de Vries, Arthur R.; Grijseels, Sietske S.
- Biotechnology for Biofuels, Vol. 9, Issue 1
Co-fermentation of xylose and cellobiose by an engineered Saccharomyces cerevisiae
journal, August 2012
- Aeling, Kimberly A.; Salmon, Kirsty A.; Laplaza, José M.
- Journal of Industrial Microbiology & Biotechnology, Vol. 39, Issue 11
Development of an industrial ethanol-producing yeast strain for efficient utilization of cellobiose
journal, June 2011
- Guo, Zhong-peng; Zhang, Liang; Ding, Zhong-yang
- Enzyme and Microbial Technology, Vol. 49, Issue 1
Energetic benefits and rapid cellobiose fermentation by Saccharomyces cerevisiae expressing cellobiose phosphorylase and mutant cellodextrin transporters
journal, January 2013
- Ha, Suk-Jin; Galazka, Jonathan M.; Joong Oh, Eun
- Metabolic Engineering, Vol. 15
Display of cellulases on the cell surface of Saccharomyces cerevisiae for high yield ethanol production from high-solid lignocellulosic biomass
journal, March 2012
- Matano, Yuki; Hasunuma, Tomohisa; Kondo, Akihiko
- Bioresource Technology, Vol. 108
Increasing Anaerobic Acetate Consumption and Ethanol Yields in Saccharomyces cerevisiae with NADPH-Specific Alcohol Dehydrogenase
journal, September 2015
- Henningsen, Brooks M.; Hon, Shuen; Covalla, Sean F.
- Applied and Environmental Microbiology, Vol. 81, Issue 23
Gene Amplification on Demand Accelerates Cellobiose Utilization in Engineered Saccharomyces cerevisiae
journal, April 2016
- Oh, Eun Joong; Skerker, Jeffrey M.; Kim, Soo Rin
- Applied and Environmental Microbiology, Vol. 82, Issue 12
Regulation of the Display Ratio of Enzymes on the Saccharomyces cerevisiae Cell Surface by the Immunoglobulin G and Cellulosomal Enzyme Binding Domains
journal, May 2009
- Ito, J.; Kosugi, A.; Tanaka, T.
- Applied and Environmental Microbiology, Vol. 75, Issue 12
Cellobiose Consumption Uncouples Extracellular Glucose Sensing and Glucose Metabolism in Saccharomyces cerevisiae
journal, August 2017
- Chomvong, Kulika; Benjamin, Daniel I.; Nomura, Daniel K.
- mBio, Vol. 8, Issue 4
Cellulosic alcoholic fermentation using recombinant Saccharomyces cerevisiae engineered for the production of Clostridium cellulovorans endoglucanase and Saccharomycopsis fibuligera β-glucosidase
journal, December 2009
- Jeon, Eugene; Hyeon, Jeong eun; Sung Eun, Lee
- FEMS Microbiology Letters, Vol. 301, Issue 1
Progress and challenges in the engineering of non-cellulolytic microorganisms for consolidated bioprocessing
journal, June 2015
- den Haan, Riaan; van Rensburg, Eugéne; Rose, Shaunita H.
- Current Opinion in Biotechnology, Vol. 33
Enhanced Bioconversion of Cellobiose by Industrial Saccharomyces cerevisiae Used for Cellulose Utilization
journal, March 2016
- Hu, Meng-Long; Zha, Jian; He, Lin-Wei
- Frontiers in Microbiology, Vol. 7
Engineering yeast for efficient cellulose degradation
journal, January 1998
- Van Rensburg, Pierre; Van Zyl, Willem H.; Pretorius, Isak S.
- Yeast, Vol. 14, Issue 1, p. 67-76
Enhanced biofuel production through coupled acetic acid and xylose consumption by engineered yeast
journal, October 2013
- Wei, Na; Quarterman, Josh; Kim, Soo Rin
- Nature Communications, Vol. 4, Issue 1
Efficient direct ethanol production from cellulose by cellulase- and cellodextrin transporter-co-expressing Saccharomyces cerevisiae
journal, January 2013
- Yamada, Ryosuke; Nakatani, Yuki; Ogino, Chiaki
- AMB Express, Vol. 3, Issue 1
Cofermentation of Cellobiose and Galactose by an Engineered Saccharomyces cerevisiae Strain
journal, June 2011
- Ha, Suk-Jin; Wei, Qiaosi; Kim, Soo Rin
- Applied and Environmental Microbiology, Vol. 77, Issue 16
Self-surface assembly of cellulosomes with two miniscaffoldins on Saccharomyces cerevisiae for cellulosic ethanol production
journal, August 2012
- Fan, L. -H.; Zhang, Z. -J.; Yu, X. -Y.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 33
Deglycosylation of cellulosomal enzyme enhances cellulosome assembly in Saccharomyces cerevisiae
journal, January 2012
- Suzuki, Hiroaki; Imaeda, Takao; Kitagawa, Takao
- Journal of Biotechnology, Vol. 157, Issue 1
Yeast Surface Display of Trifunctional Minicellulosomes for Simultaneous Saccharification and Fermentation of Cellulose to Ethanol
journal, December 2009
- Wen, F.; Sun, J.; Zhao, H.
- Applied and Environmental Microbiology, Vol. 76, Issue 4
Cellulosomes: plant-cell-wall-degrading enzyme complexes
journal, July 2004
- Doi, Roy H.; Kosugi, Akihiko
- Nature Reviews Microbiology, Vol. 2, Issue 7
Surface Display of a Functional Minicellulosome by Intracellular Complementation Using a Synthetic Yeast Consortium and Its Application to Cellulose Hydrolysis and Ethanol Production
journal, October 2010
- Tsai, S. -L.; Goyal, G.; Chen, W.
- Applied and Environmental Microbiology, Vol. 76, Issue 22
Engineering cellulolytic ability into bioprocessing organisms
journal, May 2010
- la Grange, Daniel C.; den Haan, Riaan; van Zyl, Willem H.
- Applied Microbiology and Biotechnology, Vol. 87, Issue 4
Microbial Cellulose Utilization: Fundamentals and Biotechnology
journal, September 2002
- Lynd, L. R.; Weimer, P. J.; van Zyl, W. H.
- Microbiology and Molecular Biology Reviews, Vol. 66, Issue 3, p. 506-577
Simultaneous cell growth and ethanol production from cellulose by an engineered yeast consortium displaying a functional mini-cellulosome
journal, January 2011
- Goyal, Garima; Tsai, Shen-Long; Madan, Bhawna
- Microbial Cell Factories, Vol. 10, Issue 1
Intracellular cellobiose metabolism and its applications in lignocellulose-based biorefineries
journal, September 2017
- Parisutham, Vinuselvi; Chandran, Sathesh-Prabu; Mukhopadhyay, Aindrila
- Bioresource Technology, Vol. 239
Evolutionary engineering of Saccharomyces cerevisiae for enhanced tolerance to hydrolysates of lignocellulosic biomass : Adaptive Evolution for Hydrolysates Tolerance in Yeast
journal, July 2013
- Almario, María P.; Reyes, Luis H.; Kao, Katy C.
- Biotechnology and Bioengineering, Vol. 110, Issue 10
Improved Acetic Acid Resistance in Saccharomyces cerevisiae by Overexpression of the WHI2 Gene Identified through Inverse Metabolic Engineering
journal, January 2016
- Chen, Yingying; Stabryla, Lisa; Wei, Na
- Applied and Environmental Microbiology, Vol. 82, Issue 7
Enhancement of Acetic Acid Tolerance in Saccharomyces cerevisiae by Overexpression of the HAA1 Gene, Encoding a Transcriptional Activator
journal, September 2012
- Tanaka, Koichi; Ishii, Yukari; Ogawa, Jun
- Applied and Environmental Microbiology, Vol. 78, Issue 22
Features of promising technologies for pretreatment of lignocellulosic biomass
journal, April 2005
- Mosier, Nathan; Wyman, Charles; Dale, Bruce
- Bioresource Technology, Vol. 96, Issue 6, p. 673-686
Overexpression of RCK1 improves acetic acid tolerance in Saccharomyces cerevisiae
journal, February 2019
- Oh, Eun Joong; Wei, Na; Kwak, Suryang
- Journal of Biotechnology, Vol. 292
Directed evolution of a cellobiose utilization pathway in Saccharomyces cerevisiae by simultaneously engineering multiple proteins
journal, January 2013
- Eriksen, Dawn T.; Hsieh, Pei Chiun; Lynn, Patrick
- Microbial Cell Factories, Vol. 12, Issue 1
Bio-ethanol – the fuel of tomorrow from the residues of today
journal, December 2006
- Hahn-Hägerdal, B.; Galbe, M.; Gorwa-Grauslund, M. F.
- Trends in Biotechnology, Vol. 24, Issue 12, p. 549-556
Synergetic effect of yeast cell-surface expression of cellulase and expansin-like protein on direct ethanol production from cellulose
journal, January 2013
- Nakatani, Yuki; Yamada, Ryosuke; Ogino, Chiaki
- Microbial Cell Factories, Vol. 12, Issue 1
Direct ethanol production from cellulosic materials using a diploid strain of Saccharomyces cerevisiae with optimized cellulase expression
journal, January 2011
- Yamada, Ryosuke; Taniguchi, Naho; Tanaka, Tsutomu
- Biotechnology for Biofuels, Vol. 4, Issue 1
Ethanol production from cellulosic materials using cellulase-expressing yeast
journal, March 2010
- Yanase, Shuhei; Yamada, Ryosuke; Kaneko, Shohei
- Biotechnology Journal, Vol. 5, Issue 5
Continuous co-fermentation of cellobiose and xylose by engineered Saccharomyces cerevisiae
journal, December 2013
- Ha, Suk-Jin; Kim, Soo Rin; Kim, Heejin
- Bioresource Technology, Vol. 149
Engineered Saccharomyces cerevisiae capable of simultaneous cellobiose and xylose fermentation
journal, December 2010
- Ha, S. -J.; Galazka, J. M.; Rin Kim, S.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 2
Hydrolysis and fermentation of amorphous cellulose by recombinant Saccharomyces cerevisiae
journal, January 2007
- Den Haan, Riaan; Rose, Shaunita H.; Lynd, Lee R.
- Metabolic Engineering, Vol. 9, Issue 1, p. 87-94
Improvement of ethanol production from crystalline cellulose via optimizing cellulase ratios in cellulolytic Saccharomyces cerevisiae : Higher Ethanol Yield in Cellulase-Displaying Yeast
journal, March 2017
- Liu, Zhuo; Inokuma, Kentaro; Ho, Shih-Hsin
- Biotechnology and Bioengineering, Vol. 114, Issue 6
Improvement of yeast tolerance to acetic acid through Haa1 transcription factor engineering: towards the underlying mechanisms
journal, January 2017
- Swinnen, Steve; Henriques, Sílvia F.; Shrestha, Ranjan
- Microbial Cell Factories, Vol. 16, Issue 1
Cocktail δ-integration: a novel method to construct cellulolytic enzyme expression ratio-optimized yeast strains
journal, January 2010
- Yamada, Ryosuke; Taniguchi, Naho; Tanaka, Tsutomu
- Microbial Cell Factories, Vol. 9, Issue 1
Simultaneous improvement of saccharification and ethanol production from crystalline cellulose by alleviation of irreversible adsorption of cellulase with a cell surface-engineered yeast strain
journal, November 2012
- Matano, Yuki; Hasunuma, Tomohisa; Kondo, Akihiko
- Applied Microbiology and Biotechnology, Vol. 97, Issue 5
The emergence of adaptive laboratory evolution as an efficient tool for biological discovery and industrial biotechnology
journal, December 2019
- Sandberg, Troy E.; Salazar, Michael J.; Weng, Liam L.
- Metabolic Engineering, Vol. 56
Recent Advances in Production of Bioethanol from Lignocellulosic Biomass
journal, April 2009
- Kumar, S.; Singh, S. P.; Mishra, I. M.
- Chemical Engineering & Technology, Vol. 32, Issue 4
Relief of Xylose Binding to Cellobiose Phosphorylase by a Single Distal Mutation
journal, October 2016
- Chomvong, Kulika; Lin, Eric; Blaisse, Michael
- ACS Synthetic Biology, Vol. 6, Issue 2
Improved ethanol production by engineered Saccharomyces cerevisiae expressing a mutated cellobiose transporter during simultaneous saccharification and fermentation
journal, March 2017
- Lee, Won-Heong; Jin, Yong-Su
- Journal of Biotechnology, Vol. 245
Investigate the Metabolic Reprogramming of Saccharomyces cerevisiae for Enhanced Resistance to Mixed Fermentation Inhibitors via 13C Metabolic Flux Analysis
journal, August 2016
- Guo, Weihua; Chen, Yingying; Wei, Na
- PLOS ONE, Vol. 11, Issue 8
Role and significance of beta-glucosidases in the hydrolysis of cellulose for bioethanol production
journal, January 2013
- Singhania, Reeta Rani; Patel, Anil Kumar; Sukumaran, Rajeev K.
- Bioresource Technology, Vol. 127
Directed evolution of a highly efficient cellobiose utilizing pathway in an industrial Saccharomyces cerevisiae strain : Directed Evolution of a Cellobiose Pathway
journal, June 2013
- Yuan, Yongbo; Zhao, Huimin
- Biotechnology and Bioengineering, Vol. 110, Issue 11
Evolutionary engineering strategies to enhance tolerance of xylose utilizing recombinant yeast to inhibitors derived from spruce biomass
journal, January 2012
- Koppram, Rakesh; Albers, Eva; Olsson, Lisbeth
- Biotechnology for Biofuels, Vol. 5, Issue 1
Cell recycle batch fermentation of high-solid lignocellulose using a recombinant cellulase-displaying yeast strain for high yield ethanol production in consolidated bioprocessing
journal, May 2013
- Matano, Yuki; Hasunuma, Tomohisa; Kondo, Akihiko
- Bioresource Technology, Vol. 135
Development of a D-xylose fermenting and inhibitor tolerant industrial Saccharomyces cerevisiae strain with high performance in lignocellulose hydrolysates using metabolic and evolutionary engineering
journal, January 2013
- Demeke, Mekonnen M.; Dietz, Heiko; Li, Yingying
- Biotechnology for Biofuels, Vol. 6, Issue 1
Molecular cloning and expression of fungal cellobiose transporters and β-glucosidases conferring efficient cellobiose fermentation in Saccharomyces cerevisiae
journal, January 2014
- Bae, Yi-Hyun; Kang, Kyeong-Hyeon; Jin, Yong-Su
- Journal of Biotechnology, Vol. 169