skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: A Click‐Chemistry‐Based Enrichable Crosslinker for Structural and Protein Interaction Analysis by Mass Spectrometry

Authors:
ORCiD logo [1]; ORCiD logo [2];  [2]; ORCiD logo [3]; ORCiD logo [2]
  1. Department of ChemistryLudwig-Maximilians-Universität München Butenandtstrasse 5–13 81377 Munich Germany, Department of Molecular Biology and theLewis-Sigler Institute for Integrative GenomicsPrinceton University Washington Road Princeton NJ 08544 USA
  2. Department of ChemistryLudwig-Maximilians-Universität München Butenandtstrasse 5–13 81377 Munich Germany
  3. Department of Molecular Biology and theLewis-Sigler Institute for Integrative GenomicsPrinceton University Washington Road Princeton NJ 08544 USA
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1592509
Alternate Identifier(s):
OSTI ID: 1592510
Grant/Contract Number:  
SC0018260
Resource Type:
Published Article
Journal Name:
ChemBioChem: a European journal of chemical biology
Additional Journal Information:
Journal Name: ChemBioChem: a European journal of chemical biology Journal Volume: 21 Journal Issue: 1-2; Journal ID: ISSN 1439-4227
Publisher:
Wiley Blackwell (John Wiley & Sons)
Country of Publication:
France
Language:
English

Citation Formats

Stadlmeier, Michael, Runtsch, Leander Simon, Streshnev, Filipp, Wühr, Martin, and Carell, Thomas. A Click‐Chemistry‐Based Enrichable Crosslinker for Structural and Protein Interaction Analysis by Mass Spectrometry. France: N. p., 2019. Web. doi:10.1002/cbic.201900611.
Stadlmeier, Michael, Runtsch, Leander Simon, Streshnev, Filipp, Wühr, Martin, & Carell, Thomas. A Click‐Chemistry‐Based Enrichable Crosslinker for Structural and Protein Interaction Analysis by Mass Spectrometry. France. doi:10.1002/cbic.201900611.
Stadlmeier, Michael, Runtsch, Leander Simon, Streshnev, Filipp, Wühr, Martin, and Carell, Thomas. Thu . "A Click‐Chemistry‐Based Enrichable Crosslinker for Structural and Protein Interaction Analysis by Mass Spectrometry". France. doi:10.1002/cbic.201900611.
@article{osti_1592509,
title = {A Click‐Chemistry‐Based Enrichable Crosslinker for Structural and Protein Interaction Analysis by Mass Spectrometry},
author = {Stadlmeier, Michael and Runtsch, Leander Simon and Streshnev, Filipp and Wühr, Martin and Carell, Thomas},
abstractNote = {},
doi = {10.1002/cbic.201900611},
journal = {ChemBioChem: a European journal of chemical biology},
number = 1-2,
volume = 21,
place = {France},
year = {2019},
month = {11}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1002/cbic.201900611

Save / Share:

Works referenced in this record:

Structural prediction of protein models using distance restraints derived from cross-linking mass spectrometry data
journal, February 2018

  • Orbán-Németh, Zsuzsanna; Beveridge, Rebecca; Hollenstein, David M.
  • Nature Protocols, Vol. 13, Issue 3
  • DOI: 10.1038/nprot.2017.146

Orchestrating the Biosynthesis of an Unnatural Pyrrolysine Amino Acid for Its Direct Incorporation into Proteins Inside Living Cells
journal, April 2015

  • Ehrlich, Michael; Gattner, Michael J.; Viverge, Bastien
  • Chemistry - A European Journal, Vol. 21, Issue 21
  • DOI: 10.1002/chem.201500971

xVis : a web server for the schematic visualization and interpretation of crosslink-derived spatial restraints
journal, May 2015

  • Grimm, Maximilian; Zimniak, Tomasz; Kahraman, Abdullah
  • Nucleic Acids Research, Vol. 43, Issue W1
  • DOI: 10.1093/nar/gkv463

Chances and pitfalls of chemical cross-linking with amine-reactive N-hydroxysuccinimide esters
journal, July 2008


eXL-MS: An Enhanced Cross-Linking Mass Spectrometry Workflow To Study Protein Complexes
journal, August 2018


The amyloid precursor protein (APP) binds the PIKfyve complex and modulates its function
journal, February 2016

  • Currinn, Heather; Wassmer, Thomas
  • Biochemical Society Transactions, Vol. 44, Issue 1
  • DOI: 10.1042/BST20150179

The PRIDE database and related tools and resources in 2019: improving support for quantification data
journal, November 2018

  • Perez-Riverol, Yasset; Csordas, Attila; Bai, Jingwen
  • Nucleic Acids Research, Vol. 47, Issue D1
  • DOI: 10.1093/nar/gky1106

A novel mass spectrometry-cleavable, phosphate-based enrichable and multi-targeting protein cross-linker
journal, January 2019

  • Huang, Rong; Zhu, Wei; Wu, Yue
  • Chemical Science, Vol. 10, Issue 26
  • DOI: 10.1039/C9SC00893D

Efficient and robust proteome-wide approaches for cross-linking mass spectrometry
journal, November 2018


Automated Assignment of MS/MS Cleavable Cross-Links in Protein 3D-Structure Analysis
journal, September 2014

  • Götze, Michael; Pettelkau, Jens; Fritzsche, Romy
  • Journal of The American Society for Mass Spectrometry, Vol. 26, Issue 1
  • DOI: 10.1007/s13361-014-1001-1

Polycomb Group Genes: Keeping Stem Cell Activity in Balance
journal, April 2008


Probing Native Protein Structures by Chemical Cross-linking, Mass Spectrometry, and Bioinformatics
journal, March 2010

  • Leitner, Alexander; Walzthoeni, Thomas; Kahraman, Abdullah
  • Molecular & Cellular Proteomics, Vol. 9, Issue 8
  • DOI: 10.1074/mcp.R000001-MCP201

Cross-linking mass spectrometry: methods and applications in structural, molecular and systems biology
journal, October 2018

  • O’Reilly, Francis J.; Rappsilber, Juri
  • Nature Structural & Molecular Biology, Vol. 25, Issue 11
  • DOI: 10.1038/s41594-018-0147-0

PhoX: An IMAC-Enrichable Cross-Linking Reagent
journal, August 2019

  • Steigenberger, Barbara; Pieters, Roland J.; Heck, Albert J. R.
  • ACS Central Science, Vol. 5, Issue 9
  • DOI: 10.1021/acscentsci.9b00416

Crosslinking and Mass Spectrometry: An Integrated Technology to Understand the Structure and Function of Molecular Machines
journal, January 2016

  • Leitner, Alexander; Faini, Marco; Stengel, Florian
  • Trends in Biochemical Sciences, Vol. 41, Issue 1
  • DOI: 10.1016/j.tibs.2015.10.008

Cleavable Cross-Linker for Protein Structure Analysis: Reliable Identification of Cross-Linking Products by Tandem MS
journal, August 2010

  • Müller, Mathias Q.; Dreiocker, Frank; Ihling, Christian H.
  • Analytical Chemistry, Vol. 82, Issue 16
  • DOI: 10.1021/ac101241t

NURD, a Novel Complex with Both ATP-Dependent Chromatin-Remodeling and Histone Deacetylase Activities
journal, December 1998


A new cross-linking strategy: protein interaction reporter (PIR) technology for protein–protein interaction studies
journal, January 2010

  • Tang, Xiaoting; Bruce, James E.
  • Molecular BioSystems, Vol. 6, Issue 6
  • DOI: 10.1039/b920876c

Optimized fragmentation schemes and data analysis strategies for proteome-wide cross-link identification
journal, May 2017

  • Liu, Fan; Lössl, Philip; Scheltema, Richard
  • Nature Communications, Vol. 8, Issue 1
  • DOI: 10.1038/ncomms15473

Development of a Novel Cross-linking Strategy for Fast and Accurate Identification of Cross-linked Peptides of Protein Complexes
journal, August 2010

  • Kao, Athit; Chiu, Chi-li; Vellucci, Danielle
  • Molecular & Cellular Proteomics, Vol. 10, Issue 1
  • DOI: 10.1074/mcp.M110.002212

Synthesis of two new enrichable and MS-cleavable cross-linkers to define protein–protein interactions by mass spectrometry
journal, January 2015

  • Burke, Anthony M.; Kandur, Wynne; Novitsky, Eric J.
  • Organic & Biomolecular Chemistry, Vol. 13, Issue 17
  • DOI: 10.1039/C5OB00488H

Structures of bovine, equine and leporine serum albumin
journal, September 2012


Chemical cross-linking with mass spectrometry: a tool for systems structural biology
journal, February 2019


Protein function machinery: from basic structural units to modulation of activity
journal, February 2017

  • Berezovsky, Igor N.; Guarnera, Enrico; Zheng, Zejun
  • Current Opinion in Structural Biology, Vol. 42
  • DOI: 10.1016/j.sbi.2016.10.021

A Simple Cross-Linking/Mass Spectrometry Workflow for Studying System-wide Protein Interactions
journal, July 2019