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Title: Salivary mycobiome dysbiosis and its potential impact on bacteriome shifts and host immunity in oral lichen planus

Abstract

The biodiversity of the mycobiome, an important component of the oral microbial community, and the roles of fungal–bacterial and fungal–immune system interactions in the pathogenesis of oral lichen planus (OLP) remain largely uncharacterized. In this study, we sequenced the salivary mycobiome and bacteriome associated with OLP. First, we described the dysbiosis of the microbiome in OLP patients, which exhibits lower levels of fungi and higher levels of bacteria. Significantly higher abundances of the fungi Candida and Aspergillus in patients with reticular OLP and of Alternaria and Sclerotiniaceae_unidentified in patients with erosive OLP were observed compared to the healthy controls. Aspergillus was identified as an “OLP-associated” fungus because of its detection at a higher frequency than in the healthy controls. Second, the co-occurrence patterns of the salivary mycobiome–bacteriome demonstrated negative associations between specific fungal and bacterial taxa identified in the healthy controls, which diminished in the reticular OLP group and even became positive in the erosive OLP group. Moreover, the oral cavities of OLP patients were colonized by dysbiotic oral flora with lower ecological network complexity and decreased fungal–Firmicutes and increased fungal–Bacteroidetes sub-networks. Third, several keystone fungal genera (Bovista, Erysiphe, Psathyrella, etc.) demonstrated significant correlations with clinical scores and IL-17 levels.more » Thus, we established that fungal dysbiosis is associated with the aggravation of OLP. Fungal dysbiosis could alter the salivary bacteriome or may reflect a direct effect of host immunity, which participates in OLP pathogenesis.« less

Authors:
ORCiD logo [1];  [1];  [1];  [2];  [1];  [1];  [1];  [1];  [1]; ORCiD logo [3]; ORCiD logo [3];  [4];  [1];  [5];  [1]
  1. Sichuan Univ., Chengdu (China). West China Hospital of Stomatology, National Clinical Research Center for Oral Diseases, State Key Lab. of Oral Diseases
  2. Shandong Univ., Qingdao (China). Inst. of Marine Science and Technology; Univ. of Oklahoma, Norman, OK (United States). Inst. for Environmental Genomics
  3. Univ. of Oklahoma, Norman, OK (United States). Inst. for Environmental Genomics
  4. The Forsyth Inst., Cambridge, MA (United States)
  5. Sichuan Univ., Chengdu (China). West China School of Basic Medical and Forensic Medicine
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC); National Key Research and Development Program of China; National Natural Science Foundation of China (NNSFC)
OSTI Identifier:
1582043
Grant/Contract Number:  
AC02-05CH11231; 81771085; 81430011; 81600858; 81600874; 16ZD021
Resource Type:
Accepted Manuscript
Journal Name:
International Journal of Oral Science
Additional Journal Information:
Journal Volume: 11; Journal Issue: 2; Journal ID: ISSN 1674-2818
Publisher:
Springer Nature
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Li, Yan, Wang, Kun, Zhang, Bo, Tu, Qichao, Yao, Yufei, Cui, Bomiao, Ren, Biao, He, Jinzhi, Shen, Xin, Van Nostrand, Joy D., Zhou, Jizhong, Shi, Wenyuan, Xiao, Liying, Lu, Changqing, and Zhou, Xuedong. Salivary mycobiome dysbiosis and its potential impact on bacteriome shifts and host immunity in oral lichen planus. United States: N. p., 2019. Web. doi:10.1038/s41368-019-0045-2.
Li, Yan, Wang, Kun, Zhang, Bo, Tu, Qichao, Yao, Yufei, Cui, Bomiao, Ren, Biao, He, Jinzhi, Shen, Xin, Van Nostrand, Joy D., Zhou, Jizhong, Shi, Wenyuan, Xiao, Liying, Lu, Changqing, & Zhou, Xuedong. Salivary mycobiome dysbiosis and its potential impact on bacteriome shifts and host immunity in oral lichen planus. United States. doi:10.1038/s41368-019-0045-2.
Li, Yan, Wang, Kun, Zhang, Bo, Tu, Qichao, Yao, Yufei, Cui, Bomiao, Ren, Biao, He, Jinzhi, Shen, Xin, Van Nostrand, Joy D., Zhou, Jizhong, Shi, Wenyuan, Xiao, Liying, Lu, Changqing, and Zhou, Xuedong. Tue . "Salivary mycobiome dysbiosis and its potential impact on bacteriome shifts and host immunity in oral lichen planus". United States. doi:10.1038/s41368-019-0045-2. https://www.osti.gov/servlets/purl/1582043.
@article{osti_1582043,
title = {Salivary mycobiome dysbiosis and its potential impact on bacteriome shifts and host immunity in oral lichen planus},
author = {Li, Yan and Wang, Kun and Zhang, Bo and Tu, Qichao and Yao, Yufei and Cui, Bomiao and Ren, Biao and He, Jinzhi and Shen, Xin and Van Nostrand, Joy D. and Zhou, Jizhong and Shi, Wenyuan and Xiao, Liying and Lu, Changqing and Zhou, Xuedong},
abstractNote = {The biodiversity of the mycobiome, an important component of the oral microbial community, and the roles of fungal–bacterial and fungal–immune system interactions in the pathogenesis of oral lichen planus (OLP) remain largely uncharacterized. In this study, we sequenced the salivary mycobiome and bacteriome associated with OLP. First, we described the dysbiosis of the microbiome in OLP patients, which exhibits lower levels of fungi and higher levels of bacteria. Significantly higher abundances of the fungi Candida and Aspergillus in patients with reticular OLP and of Alternaria and Sclerotiniaceae_unidentified in patients with erosive OLP were observed compared to the healthy controls. Aspergillus was identified as an “OLP-associated” fungus because of its detection at a higher frequency than in the healthy controls. Second, the co-occurrence patterns of the salivary mycobiome–bacteriome demonstrated negative associations between specific fungal and bacterial taxa identified in the healthy controls, which diminished in the reticular OLP group and even became positive in the erosive OLP group. Moreover, the oral cavities of OLP patients were colonized by dysbiotic oral flora with lower ecological network complexity and decreased fungal–Firmicutes and increased fungal–Bacteroidetes sub-networks. Third, several keystone fungal genera (Bovista, Erysiphe, Psathyrella, etc.) demonstrated significant correlations with clinical scores and IL-17 levels. Thus, we established that fungal dysbiosis is associated with the aggravation of OLP. Fungal dysbiosis could alter the salivary bacteriome or may reflect a direct effect of host immunity, which participates in OLP pathogenesis.},
doi = {10.1038/s41368-019-0045-2},
journal = {International Journal of Oral Science},
number = 2,
volume = 11,
place = {United States},
year = {2019},
month = {7}
}

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