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Title: Persistent in vivo cytogenetic effects of radioiodine therapy: a 21-year follow-up study using multicolor FISH

Abstract

Abstract Our previous studies demonstrated the cytogenetic effects in the peripheral blood lymphocytes of a 34-year-old male patient who received ablative radioactive 131iodine therapy (RIT) on two different occasions in 1992 and 1994. Assessment of RIT-induced chromosomal damage by the cytokinesis-blocked micronucleus assay (CBMN) showed the persistence of elevated micronucleus frequency in this patient for more than two decades since the first RIT. Subsequent cytogenetic analysis performed in 2012 revealed both stable and unstable aberrations, whose frequencies were higher than the baseline reported in the literature. Here, we report the findings of our recent cytogenetic analysis peformed in 2015 on this patient using the multicolor fluorescence in situ hybridization (mFISH) technique. Our results showed that both reciprocal and non-reciprocal translocations persisted at higher frequencies in the patient than those reported in 2012. Persistence of structural aberrations for more than two decades indicate that these aberrations might have originated from long-lived T-lymphocytes or hematopoietic stem cells. Our study suggests that the long-term persistence of chromosome translocations in circulating lymphocytes can be useful for monitoring the extent of RIT-induced chromosomal instability several years after exposure and for estimating the cumulative absorbed dose after multiple RITs for retrospective biodosimetry purposes. This is perhapsmore » the first and longest follow-up study documenting the persistence of cytogenetic damage for 21 years after internal radiation exposure.« less

Authors:
 [1];  [1];  [2];  [1]
  1. Oak Ridge Associated Univ., Oak Ridge, TN (United States). Radiation Emergency Assistance Center and Training Site, Cytogenetic Biodosimetry Lab.
  2. Indiana Univ. Health, Ball Memorial Hospital, Muncie, IN (United States)
Publication Date:
Research Org.:
Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1581830
Alternate Identifier(s):
OSTI ID: 1580743
Grant/Contract Number:  
SC0014664; AC05-06OR23100
Resource Type:
Published Article
Journal Name:
Journal of Radiation Research
Additional Journal Information:
Journal Volume: 59; Journal Issue: 1; Journal ID: ISSN 0449-3060
Publisher:
Oxford University Press
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; in vivo exposure; 131Iodine; multicolor fluorescence in situ hybridization; chromosomal translocations; micronuclei; telomere and centromere FISH analysis

Citation Formats

Livingston, Gordon K., Escalona, Maria, Foster, Alvis, and Balajee, Adayabalam S. Persistent in vivo cytogenetic effects of radioiodine therapy: a 21-year follow-up study using multicolor FISH. United States: N. p., 2017. Web. doi:10.1093/jrr/rrx049.
Livingston, Gordon K., Escalona, Maria, Foster, Alvis, & Balajee, Adayabalam S. Persistent in vivo cytogenetic effects of radioiodine therapy: a 21-year follow-up study using multicolor FISH. United States. doi:10.1093/jrr/rrx049.
Livingston, Gordon K., Escalona, Maria, Foster, Alvis, and Balajee, Adayabalam S. Tue . "Persistent in vivo cytogenetic effects of radioiodine therapy: a 21-year follow-up study using multicolor FISH". United States. doi:10.1093/jrr/rrx049.
@article{osti_1581830,
title = {Persistent in vivo cytogenetic effects of radioiodine therapy: a 21-year follow-up study using multicolor FISH},
author = {Livingston, Gordon K. and Escalona, Maria and Foster, Alvis and Balajee, Adayabalam S.},
abstractNote = {Abstract Our previous studies demonstrated the cytogenetic effects in the peripheral blood lymphocytes of a 34-year-old male patient who received ablative radioactive 131iodine therapy (RIT) on two different occasions in 1992 and 1994. Assessment of RIT-induced chromosomal damage by the cytokinesis-blocked micronucleus assay (CBMN) showed the persistence of elevated micronucleus frequency in this patient for more than two decades since the first RIT. Subsequent cytogenetic analysis performed in 2012 revealed both stable and unstable aberrations, whose frequencies were higher than the baseline reported in the literature. Here, we report the findings of our recent cytogenetic analysis peformed in 2015 on this patient using the multicolor fluorescence in situ hybridization (mFISH) technique. Our results showed that both reciprocal and non-reciprocal translocations persisted at higher frequencies in the patient than those reported in 2012. Persistence of structural aberrations for more than two decades indicate that these aberrations might have originated from long-lived T-lymphocytes or hematopoietic stem cells. Our study suggests that the long-term persistence of chromosome translocations in circulating lymphocytes can be useful for monitoring the extent of RIT-induced chromosomal instability several years after exposure and for estimating the cumulative absorbed dose after multiple RITs for retrospective biodosimetry purposes. This is perhaps the first and longest follow-up study documenting the persistence of cytogenetic damage for 21 years after internal radiation exposure.},
doi = {10.1093/jrr/rrx049},
journal = {Journal of Radiation Research},
number = 1,
volume = 59,
place = {United States},
year = {2017},
month = {9}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1093/jrr/rrx049

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Cited by: 2 works
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