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Title: Cost minimization of deoxygenation for control of Vampirovibrio chlorellavorus in Chlorella sorokiniana cultures

Abstract

Deoxygenation is a strategy for preventing Vampirovibrio chlorellavorus infection of Chlorella sorokiniana cultures. Deoxygenation cost was minimized by sparging with nitrogen gas for only one hour at the onset of night and relying on naturally-occurring dark-respiration to maintain low oxygen concentrations throughout the night. This technique substantially reduced V. chlorellavorus infection in laboratory co-cultures. The cost of the approach was evaluated in outdoor experiments with one pure water and one pathogen-free C. sorokiniana culture, grown in a 150-L sealed, vented, translucent-polyethylene reactor. Unlike the small laboratory reactors, which were also sealed and vented, the much larger outdoor reactor maintained the dissolved oxygen concentration in the C. sorokiniana culture at near zero concentration throughout the night cycle. This demonstrates that large covered tanks in commercial applications would keep oxygen concentration near zero for the entire night. The total nitrogen sparged, per night, per liter of algal culture, was determined for the 150-L reactor, and used to estimate the cost per m2 for large scale raceways. Onsite nitrogen generators are the most cost-effective method to supply a high flow of nitrogen gas to commercial scale raceways. The cost of deoxygenation treatment ranged from $16/ton AFDW algae for a shallow (2 cm) gravitymore » flow system with 75% harvest prior to deoxygenation to over $1,300/ton for a 20 cm depth raceway with plastic tanks.« less

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1]
  1. Univ. of Arizona, Tucson, AZ (United States)
Publication Date:
Research Org.:
Univ. of Arizona, Tucson, AZ (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
OSTI Identifier:
1581780
Alternate Identifier(s):
OSTI ID: 1543355
Grant/Contract Number:  
ee0006269
Resource Type:
Accepted Manuscript
Journal Name:
Algal Research
Additional Journal Information:
Journal Volume: 42; Journal Issue: C; Journal ID: ISSN 2211-9264
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Aeration; Anoxic; Algal biomass; Bacterial infection; Economic feasibility; Nitrogen sparging

Citation Formats

Attalah, S., Waller, P., Steichen, S., Brown, C. C., Mehdipour, Y., Ogden, K., and Brown, J. K. Cost minimization of deoxygenation for control of Vampirovibrio chlorellavorus in Chlorella sorokiniana cultures. United States: N. p., 2019. Web. doi:10.1016/j.algal.2019.101615.
Attalah, S., Waller, P., Steichen, S., Brown, C. C., Mehdipour, Y., Ogden, K., & Brown, J. K. Cost minimization of deoxygenation for control of Vampirovibrio chlorellavorus in Chlorella sorokiniana cultures. United States. doi:10.1016/j.algal.2019.101615.
Attalah, S., Waller, P., Steichen, S., Brown, C. C., Mehdipour, Y., Ogden, K., and Brown, J. K. Sun . "Cost minimization of deoxygenation for control of Vampirovibrio chlorellavorus in Chlorella sorokiniana cultures". United States. doi:10.1016/j.algal.2019.101615.
@article{osti_1581780,
title = {Cost minimization of deoxygenation for control of Vampirovibrio chlorellavorus in Chlorella sorokiniana cultures},
author = {Attalah, S. and Waller, P. and Steichen, S. and Brown, C. C. and Mehdipour, Y. and Ogden, K. and Brown, J. K.},
abstractNote = {Deoxygenation is a strategy for preventing Vampirovibrio chlorellavorus infection of Chlorella sorokiniana cultures. Deoxygenation cost was minimized by sparging with nitrogen gas for only one hour at the onset of night and relying on naturally-occurring dark-respiration to maintain low oxygen concentrations throughout the night. This technique substantially reduced V. chlorellavorus infection in laboratory co-cultures. The cost of the approach was evaluated in outdoor experiments with one pure water and one pathogen-free C. sorokiniana culture, grown in a 150-L sealed, vented, translucent-polyethylene reactor. Unlike the small laboratory reactors, which were also sealed and vented, the much larger outdoor reactor maintained the dissolved oxygen concentration in the C. sorokiniana culture at near zero concentration throughout the night cycle. This demonstrates that large covered tanks in commercial applications would keep oxygen concentration near zero for the entire night. The total nitrogen sparged, per night, per liter of algal culture, was determined for the 150-L reactor, and used to estimate the cost per m2 for large scale raceways. Onsite nitrogen generators are the most cost-effective method to supply a high flow of nitrogen gas to commercial scale raceways. The cost of deoxygenation treatment ranged from $16/ton AFDW algae for a shallow (2 cm) gravity flow system with 75% harvest prior to deoxygenation to over $1,300/ton for a 20 cm depth raceway with plastic tanks.},
doi = {10.1016/j.algal.2019.101615},
journal = {Algal Research},
number = C,
volume = 42,
place = {United States},
year = {2019},
month = {9}
}

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