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Title: Application of deoxygenation-aeration cycling to control the predatory bacterium Vampirovibrio chlorellavorus in Chlorella sorokiniana cultures

Abstract

A previously untested approach was evaluated to enable management of the predatory bacterium, Vampirovibrio chlorellavorus, a pathogen of Chlorella sorokiniana, in suspension cultures grown in a laboratory test reactor. Because V. chlorellavorus is an obligate aerobic bacterium, whereas C. sorokiniana grows under aerobic and anaerobic conditions, deoxygenation of the culture was expected to be detrimental to the pathogen, but not to the algal host. The effect of deoxygenation on the uninfected (healthy) C. sorokiniana suspension cells, compared to the C. sorokiniana-V. chlorellavorus co-culture, was studied in relation to biomass, dissolved oxygen, ratio of C. sorokiniana to V. chlorellavorus DNA, and visual and light microscopic observations. Preliminary experiments were conducted to test the effects of different deoxygenation-aeration cycling regimes on performance of V. chlorellavorus-free C. sorokiniana cultures. To an aerobic culture, pure nitrogen gas was introduced to create anoxic conditions, followed by the injection of ambient air to re-establish an aerobic environment. Under this repeated cycling regime, C. sorokiniana was shown to tolerate the anoxic conditions for extended timespans that ranged from 2 to 8 hours over a 5-day test period. The analogous aerobic-anoxic cycling with the C. sorokiniana-V. chlorellavorus co-cultures resulted in ‘near-normal’ growth cycle and harvestable biomass, whereas themore » continuously-aerated (aerobic) co-cultures that were grown without the deoxygenation step in the cycle collapsed in 3 days. Visual and light microscopic observations revealed intact C. sorokiniana cells were present in the deoxygenated cultures, compared to the aerobically-grown, brown-colored algal cultures consisting of collapsed cells. Quantitative polymerase chain reaction analysis showed continuous increases in the ratio of V. chlorellavorus (16S rDNA) to C. sorokiniana (18S rDNA) DNA in the aerated co-cultures, with greater increases during dark periods, while the pathogen-to-host DNA ratio in the deoxygenated co-cultures was relatively low and algal cells did not collapse, as would be expected following pathogen attack.« less

Authors:
 [1];  [1];  [1];  [2];  [1];  [1];  [1]
  1. Univ. of Arizona, Tucson, AZ (United States)
  2. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Publication Date:
Research Org.:
Univ. of Arizona, Tucson, AZ (United States); Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office
OSTI Identifier:
1581775
Grant/Contract Number:  
ee0006269
Resource Type:
Accepted Manuscript
Journal Name:
Algal Research
Additional Journal Information:
Journal Volume: 39; Journal Issue: C; Journal ID: ISSN 2211-9264
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Algae; Anoxic; Biomass; Infection; Nitrogen; Quantitative polymerase chain reaction

Citation Formats

Attalah, Said, Waller, Peter, Steichen, Seth, Gao, Song, Brown, Caitlin, Ogden, Kimberly, and Brown, Judy. Application of deoxygenation-aeration cycling to control the predatory bacterium Vampirovibrio chlorellavorus in Chlorella sorokiniana cultures. United States: N. p., 2019. Web. https://doi.org/10.1016/j.algal.2019.101427.
Attalah, Said, Waller, Peter, Steichen, Seth, Gao, Song, Brown, Caitlin, Ogden, Kimberly, & Brown, Judy. Application of deoxygenation-aeration cycling to control the predatory bacterium Vampirovibrio chlorellavorus in Chlorella sorokiniana cultures. United States. https://doi.org/10.1016/j.algal.2019.101427
Attalah, Said, Waller, Peter, Steichen, Seth, Gao, Song, Brown, Caitlin, Ogden, Kimberly, and Brown, Judy. Wed . "Application of deoxygenation-aeration cycling to control the predatory bacterium Vampirovibrio chlorellavorus in Chlorella sorokiniana cultures". United States. https://doi.org/10.1016/j.algal.2019.101427. https://www.osti.gov/servlets/purl/1581775.
@article{osti_1581775,
title = {Application of deoxygenation-aeration cycling to control the predatory bacterium Vampirovibrio chlorellavorus in Chlorella sorokiniana cultures},
author = {Attalah, Said and Waller, Peter and Steichen, Seth and Gao, Song and Brown, Caitlin and Ogden, Kimberly and Brown, Judy},
abstractNote = {A previously untested approach was evaluated to enable management of the predatory bacterium, Vampirovibrio chlorellavorus, a pathogen of Chlorella sorokiniana, in suspension cultures grown in a laboratory test reactor. Because V. chlorellavorus is an obligate aerobic bacterium, whereas C. sorokiniana grows under aerobic and anaerobic conditions, deoxygenation of the culture was expected to be detrimental to the pathogen, but not to the algal host. The effect of deoxygenation on the uninfected (healthy) C. sorokiniana suspension cells, compared to the C. sorokiniana-V. chlorellavorus co-culture, was studied in relation to biomass, dissolved oxygen, ratio of C. sorokiniana to V. chlorellavorus DNA, and visual and light microscopic observations. Preliminary experiments were conducted to test the effects of different deoxygenation-aeration cycling regimes on performance of V. chlorellavorus-free C. sorokiniana cultures. To an aerobic culture, pure nitrogen gas was introduced to create anoxic conditions, followed by the injection of ambient air to re-establish an aerobic environment. Under this repeated cycling regime, C. sorokiniana was shown to tolerate the anoxic conditions for extended timespans that ranged from 2 to 8 hours over a 5-day test period. The analogous aerobic-anoxic cycling with the C. sorokiniana-V. chlorellavorus co-cultures resulted in ‘near-normal’ growth cycle and harvestable biomass, whereas the continuously-aerated (aerobic) co-cultures that were grown without the deoxygenation step in the cycle collapsed in 3 days. Visual and light microscopic observations revealed intact C. sorokiniana cells were present in the deoxygenated cultures, compared to the aerobically-grown, brown-colored algal cultures consisting of collapsed cells. Quantitative polymerase chain reaction analysis showed continuous increases in the ratio of V. chlorellavorus (16S rDNA) to C. sorokiniana (18S rDNA) DNA in the aerated co-cultures, with greater increases during dark periods, while the pathogen-to-host DNA ratio in the deoxygenated co-cultures was relatively low and algal cells did not collapse, as would be expected following pathogen attack.},
doi = {10.1016/j.algal.2019.101427},
journal = {Algal Research},
number = C,
volume = 39,
place = {United States},
year = {2019},
month = {5}
}

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Works referenced in this record:

Influence of irradiance, dissolved oxygen concentration, and temperature on the growth of Chlorella sorokiniana
journal, June 2007


The algae raceway integrated design for optimal temperature management
journal, November 2012


Real-time quantitative detection of Vampirovibrio chlorellavorus, an obligate bacterial pathogen of Chlorella sorokiniana
journal, October 2018


Incorporation of salinity, nitrogen, and shading stress factors into the Huesemann Algae Biomass Growth model
journal, November 2018


Effects of Light and Temperature on Fatty Acid Production in Nannochloropsis Salina
journal, March 2012

  • Van Wagenen, Jon; Miller, Tyler W.; Hobbs, Sam
  • Energies, Vol. 5, Issue 3
  • DOI: 10.3390/en5030731

Potential of nitrogen gas (N2) to control psychrotrophs and mesophiles in raw milk
journal, February 2010

  • Munsch-Alatossava, Patricia; Gursoy, Oguz; Alatossava, Tapani
  • Microbiological Research, Vol. 165, Issue 2
  • DOI: 10.1016/j.micres.2009.02.002

Review of the cultivation program within the National Alliance for Advanced Biofuels and Bioproducts
journal, March 2017


Ultrahigh-cell-density culture of a marine green alga Chlorococcum littorale in a flat-plate photobioreactor
journal, June 1998

  • Hu, Q.; Kurano, N.; Kawachi, M.
  • Applied Microbiology and Biotechnology, Vol. 49, Issue 6
  • DOI: 10.1007/s002530051228

Effect of biomass concentration on the productivity of Tetraselmis suecica in a pilot-scale tubular photobioreactor using natural sunlight
journal, April 2014


Antagonistic association of the chlorellavorus bacterium (“Bdellovibrio” chlorellavorus) withChlorella vulgaris
journal, January 1978

  • Coder, David M.; Starr, Mortimer P.
  • Current Microbiology, Vol. 1, Issue 1
  • DOI: 10.1007/BF02601710

Night biomass loss and changes in biochemical composition of cells during light/dark cyclic culture of Chlorella pyrenoidosa
journal, January 1996


Parasites in algae mass culture
journal, June 2014


A Novel Treatment Protects Chlorella at Commercial Scale from the Predatory Bacterium Vampirovibrio chlorellavorus
journal, June 2016

  • Ganuza, Eneko; Sellers, Charles E.; Bennett, Braden W.
  • Frontiers in Microbiology, Vol. 7
  • DOI: 10.3389/fmicb.2016.00848

Phlyctidium scenedesmi spec. nova, a new chytrid destroying mass cultures of algae
journal, January 1967


Industrial-strength ecology: trade-offs and opportunities in algal biofuel production
journal, September 2013

  • Shurin, Jonathan B.; Abbott, Rachel L.; Deal, Michael S.
  • Ecology Letters, Vol. 16, Issue 11
  • DOI: 10.1111/ele.12176

Fungicide effect of Triton-N on Phlyctidium
journal, May 1993

  • Benderliev, K. M.; Pouneva, I. D.; Ivanova, N. I.
  • Biotechnology Techniques, Vol. 7, Issue 5
  • DOI: 10.1007/BF00152537

Controlling wildlife fungal disease spread: in vitro efficacy of disinfectants against Batrachochytrium dendrobatidis and Mucor amphibiorum
journal, June 2012

  • Webb, R.; Philips, A.; Speare, R.
  • Diseases of Aquatic Organisms, Vol. 99, Issue 2
  • DOI: 10.3354/dao02461

Discovery of Bioactive Metabolites in Biofuel Microalgae That Offer Protection against Predatory Bacteria
journal, April 2016

  • Bagwell, Christopher E.; Abernathy, Amanda; Barnwell, Remy
  • Frontiers in Microbiology, Vol. 7
  • DOI: 10.3389/fmicb.2016.00516

Sonication of bacteria, phytoplankton and zooplankton: Application to treatment of ballast water
journal, June 2008


Selection, breeding and engineering of microalgae for bioenergy and biofuel production
journal, April 2012


Advances in genetic engineering of marine algae
journal, November 2012


Morphology, composition, production, processing and applications of Chlorella vulgaris: A review
journal, July 2014


Microbial removal of hazardous organic compounds
journal, March 1982

  • Kobayashi, Hester; Rittmann, Bruce E.
  • Environmental Science & Technology, Vol. 16, Issue 3
  • DOI: 10.1021/es00097a002

The host Range of the Chlorellavorous Bacterium ("Vampirovibrio Chlorellvorus")
journal, December 1986


Electron microscope study of the interaction of epibiontic bacteria withChromatium minus in natural habitats
journal, April 1983

  • Esteve, I.; Guerrero, R.; Montesinos, E.
  • Microbial Ecology, Vol. 9, Issue 1
  • DOI: 10.1007/BF02011580

Back from the dead; the curious tale of the predatory cyanobacterium Vampirovibrio chlorellavorus
journal, January 2015

  • Soo, Rochelle M.; Woodcroft, Ben J.; Parks, Donovan H.
  • PeerJ, Vol. 3
  • DOI: 10.7717/peerj.968

An Improved Gas-Stripping Column for Deoxygenating Water
journal, June 1995

  • Barnhart, M. Christopher
  • Journal of the North American Benthological Society, Vol. 14, Issue 2
  • DOI: 10.2307/1467786

Some Aspects of the Endogenous Metabolism of Bacteria
journal, June 1964


Microbiological Endogenous Processes in Biological Wastewater Treatment Systems
journal, February 2010

  • Hao, Xiao-Di; Wang, Qi-Lin; Zhu, Jing-Yi
  • Critical Reviews in Environmental Science and Technology, Vol. 40, Issue 3
  • DOI: 10.1080/10643380802278901

Breathing life into pathogens: the influence of oxygen on bacterial virulence and host responses in the gastrointestinal tract: Oxygen and intestinal pathogens
journal, December 2010


    Works referencing / citing this record:

    Sensitivity of predatory bacteria to different surfactants and their application to check bacterial predation
    journal, August 2019

    • Cho, Gayoung; Kwon, Jisoo; Soh, Sandrine Mabekou
    • Applied Microbiology and Biotechnology, Vol. 103, Issue 19
    • DOI: 10.1007/s00253-019-10069-w