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Title: Tentacle Probes™: Differentiation of Difficult Single-Nucleotide Polymorphisms and Deletions by Presence or Absence of a Signal in Real-Time PCR

Abstract

Abstract Background: False-positive results are a common problem in real-time PCR identification of DNA sequences that differ from near neighbors by a single-nucleotide polymorphism (SNP) or deletion. Because of a lack of sufficient probe specificity, post-PCR analysis, such as a melting curve, is often required for mutation differentiation. Methods: Tentacle Probes™, cooperative reagents with both a capture and a detection probe based on specific cell-targeting principles, were developed as a replacement for 2 chromosomal TaqMan–minor groove binder (MGB) assays previously developed for Yersinia pestis and Bacillus anthracis detection. We compared TaqMan-MGB probes to Tentacle Probes for SNP and deletion detection based on the presence or absence of a growth curve. Results: With the TaqMan-MGB Y. pestis yp48 assays, false-positive results for Yersinia pseudotuberculosis occurred at every concentration tested, and with the TaqMan-MGB B. anthracis gyrA assays, false-positive results occurred in 21 of 29 boil preps of environmental samples of near neighbors. With Tentacle Probes no false-positive results occurred. Conclusions: The high specificity exhibited by Tentacle Probes may eliminate melting curve analysis for SNP and deletion mutation detection, allowing the diagnostic use of previously difficult targets.

Authors:
 [1];  [2];  [2];  [2];  [3];  [4]
  1. Harrington Department of Bioengineering, Arizona State University, Tempe, AZ, Arcxis Biotechnologies, Pleasanton, CA
  2. United States Army Medical Research Institute of Infectious Diseases, Frederick, MD
  3. Harrington Department of Bioengineering, Arizona State University, Tempe, AZ
  4. Arcxis Biotechnologies, Pleasanton, CA
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1581655
Grant/Contract Number:  
AC05-00OR22750; NBCHC060031
Resource Type:
Published Article
Journal Name:
Clinical Chemistry
Additional Journal Information:
Journal Name: Clinical Chemistry Journal Volume: 53 Journal Issue: 12; Journal ID: ISSN 0009-9147
Publisher:
Oxford University Press
Country of Publication:
United States
Language:
English

Citation Formats

Satterfield, Brent C., Kulesh, David A., Norwood, David A., Wasieloski, Jr, Leonard P., Caplan, Michael R., and West, Jay AA. Tentacle Probes™: Differentiation of Difficult Single-Nucleotide Polymorphisms and Deletions by Presence or Absence of a Signal in Real-Time PCR. United States: N. p., 2020. Web. doi:10.1373/clinchem.2007.091488.
Satterfield, Brent C., Kulesh, David A., Norwood, David A., Wasieloski, Jr, Leonard P., Caplan, Michael R., & West, Jay AA. Tentacle Probes™: Differentiation of Difficult Single-Nucleotide Polymorphisms and Deletions by Presence or Absence of a Signal in Real-Time PCR. United States. doi:10.1373/clinchem.2007.091488.
Satterfield, Brent C., Kulesh, David A., Norwood, David A., Wasieloski, Jr, Leonard P., Caplan, Michael R., and West, Jay AA. Wed . "Tentacle Probes™: Differentiation of Difficult Single-Nucleotide Polymorphisms and Deletions by Presence or Absence of a Signal in Real-Time PCR". United States. doi:10.1373/clinchem.2007.091488.
@article{osti_1581655,
title = {Tentacle Probes™: Differentiation of Difficult Single-Nucleotide Polymorphisms and Deletions by Presence or Absence of a Signal in Real-Time PCR},
author = {Satterfield, Brent C. and Kulesh, David A. and Norwood, David A. and Wasieloski, Jr, Leonard P. and Caplan, Michael R. and West, Jay AA},
abstractNote = {Abstract Background: False-positive results are a common problem in real-time PCR identification of DNA sequences that differ from near neighbors by a single-nucleotide polymorphism (SNP) or deletion. Because of a lack of sufficient probe specificity, post-PCR analysis, such as a melting curve, is often required for mutation differentiation. Methods: Tentacle Probes™, cooperative reagents with both a capture and a detection probe based on specific cell-targeting principles, were developed as a replacement for 2 chromosomal TaqMan–minor groove binder (MGB) assays previously developed for Yersinia pestis and Bacillus anthracis detection. We compared TaqMan-MGB probes to Tentacle Probes for SNP and deletion detection based on the presence or absence of a growth curve. Results: With the TaqMan-MGB Y. pestis yp48 assays, false-positive results for Yersinia pseudotuberculosis occurred at every concentration tested, and with the TaqMan-MGB B. anthracis gyrA assays, false-positive results occurred in 21 of 29 boil preps of environmental samples of near neighbors. With Tentacle Probes no false-positive results occurred. Conclusions: The high specificity exhibited by Tentacle Probes may eliminate melting curve analysis for SNP and deletion mutation detection, allowing the diagnostic use of previously difficult targets.},
doi = {10.1373/clinchem.2007.091488},
journal = {Clinical Chemistry},
number = 12,
volume = 53,
place = {United States},
year = {2020},
month = {1}
}

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