The genetic basis of aneuploidy tolerance in wild yeast
Abstract
Aneuploidy is highly detrimental during development yet common in cancers and pathogenic fungi – what gives rise to differences in aneuploidy tolerance remains unclear. We previously showed that wild isolates of Saccharomyces cerevisiae tolerate chromosome amplification while laboratory strains used as a model for aneuploid syndromes do not. Here, we mapped the genetic basis to Ssd1, an RNA-binding translational regulator that is functional in wild aneuploids but defective in laboratory strain W303. Loss of SSD1 recapitulates myriad aneuploidy signatures previously taken as eukaryotic responses. We show that aneuploidy tolerance is enabled via a role for Ssd1 in mitochondrial physiology, including binding and regulating nuclear-encoded mitochondrial mRNAs, coupled with a role in mitigating proteostasis stress. Recapitulating ssd1Δ defects with combinatorial drug treatment selectively blocked proliferation of wild-type aneuploids compared to euploids. Our work adds to elegant studies in the sensitized laboratory strain to present a mechanistic understanding of eukaryotic aneuploidy tolerance.
- Authors:
-
[7]
- Center for Genomic Science Innovation, University of Wisconsin–Madison, Madison, United States
- Center for Genomic Science Innovation, University of Wisconsin–Madison, Madison, United States, Laboratory of Genetics, University of Wisconsin-Madison, Madison, United States
- Laboratory of Genetics, University of Wisconsin-Madison, Madison, United States
- Center for Genomic Science Innovation, University of Wisconsin–Madison, Madison, United States, Great Lakes Bioenergy Research Center, Madison, United States
- Center for Genomic Science Innovation, University of Wisconsin–Madison, Madison, United States, Great Lakes Bioenergy Research Center, Madison, United States, Department of Biomolecular Chemistry, University of Wisconsin–Madison, Madison, United States, Department of Chemistry, University of Wisconsin–Madison, Madison, United States, Morgridge Institute for Research, Madison, United States
- Center for Genomic Science Innovation, University of Wisconsin–Madison, Madison, United States, Morgridge Institute for Research, Madison, United States
- Center for Genomic Science Innovation, University of Wisconsin–Madison, Madison, United States, Laboratory of Genetics, University of Wisconsin-Madison, Madison, United States, Great Lakes Bioenergy Research Center, Madison, United States
- Publication Date:
- Research Org.:
- Univ. of Wisconsin, Madison, WI (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1581463
- Alternate Identifier(s):
- OSTI ID: 1581464; OSTI ID: 1803406
- Grant/Contract Number:
- SC0018409
- Resource Type:
- Published Article
- Journal Name:
- eLife
- Additional Journal Information:
- Journal Name: eLife Journal Volume: 9; Journal ID: ISSN 2050-084X
- Publisher:
- eLife Sciences Publications, Ltd.
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; chromosomes and gene expression; aneuploidy; natural variation; proteotoxicity; wild strains
Citation Formats
Hose, James, Escalante, Leah E., Clowers, Katie J., Dutcher, H. Auguste, Robinson, DeElegant, Bouriakov, Venera, Coon, Joshua J., Shishkova, Evgenia, and Gasch, Audrey P. The genetic basis of aneuploidy tolerance in wild yeast. United States: N. p., 2020.
Web. doi:10.7554/eLife.52063.
Hose, James, Escalante, Leah E., Clowers, Katie J., Dutcher, H. Auguste, Robinson, DeElegant, Bouriakov, Venera, Coon, Joshua J., Shishkova, Evgenia, & Gasch, Audrey P. The genetic basis of aneuploidy tolerance in wild yeast. United States. https://doi.org/10.7554/eLife.52063
Hose, James, Escalante, Leah E., Clowers, Katie J., Dutcher, H. Auguste, Robinson, DeElegant, Bouriakov, Venera, Coon, Joshua J., Shishkova, Evgenia, and Gasch, Audrey P. Tue .
"The genetic basis of aneuploidy tolerance in wild yeast". United States. https://doi.org/10.7554/eLife.52063.
@article{osti_1581463,
title = {The genetic basis of aneuploidy tolerance in wild yeast},
author = {Hose, James and Escalante, Leah E. and Clowers, Katie J. and Dutcher, H. Auguste and Robinson, DeElegant and Bouriakov, Venera and Coon, Joshua J. and Shishkova, Evgenia and Gasch, Audrey P.},
abstractNote = {Aneuploidy is highly detrimental during development yet common in cancers and pathogenic fungi – what gives rise to differences in aneuploidy tolerance remains unclear. We previously showed that wild isolates of Saccharomyces cerevisiae tolerate chromosome amplification while laboratory strains used as a model for aneuploid syndromes do not. Here, we mapped the genetic basis to Ssd1, an RNA-binding translational regulator that is functional in wild aneuploids but defective in laboratory strain W303. Loss of SSD1 recapitulates myriad aneuploidy signatures previously taken as eukaryotic responses. We show that aneuploidy tolerance is enabled via a role for Ssd1 in mitochondrial physiology, including binding and regulating nuclear-encoded mitochondrial mRNAs, coupled with a role in mitigating proteostasis stress. Recapitulating ssd1Δ defects with combinatorial drug treatment selectively blocked proliferation of wild-type aneuploids compared to euploids. Our work adds to elegant studies in the sensitized laboratory strain to present a mechanistic understanding of eukaryotic aneuploidy tolerance.},
doi = {10.7554/eLife.52063},
journal = {eLife},
number = ,
volume = 9,
place = {United States},
year = {2020},
month = {1}
}
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