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Title: Intrinsically disordered proteins access a range of hysteretic phase separation behaviors

Abstract

The phase separation behavior of intrinsically disordered proteins (IDPs) is thought of as analogous to that of polymers that undergo equilibrium lower or upper critical solution temperature (LCST and UCST, respectively) phase transition. This view, however, ignores possible nonequilibrium properties of protein assemblies. Here, by studying IDP polymers (IDPPs) composed of repeat motifs that encode LCST or UCST phase behavior, we discovered that IDPs can access a wide spectrum of nonequilibrium, hysteretic phase behaviors. Experimentally and through simulations, we show that hysteresis in IDPPs is tunable and that it emerges through increasingly stable interchain interactions in the insoluble phase. To explore the utility of hysteretic IDPPs, we engineer self-assembling nanostructures with tunable stability. These findings shine light on the rich phase separation behavior of IDPs and illustrate hysteresis as a design parameter to program nonequilibrium phase behavior in self-assembling materials.

Authors:
ORCiD logo [1]; ORCiD logo [2]; ORCiD logo [1];  [3]; ORCiD logo [1];  [1]; ORCiD logo [2]; ORCiD logo [1]
  1. Duke Univ., Durham, NC (United States)
  2. North Carolina State Univ., Raleigh, NC (United States)
  3. Univ. of Basel (Switzerland). Swiss Nanoscience Inst.
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC); National Institutes of Health (NIH); National Science Foundation (NSF)
OSTI Identifier:
1580951
Grant/Contract Number:  
AC02-05CH11231; R01 GM061232; MIRA R35GM127042; NSF-DMR-1729671
Resource Type:
Accepted Manuscript
Journal Name:
Science Advances
Additional Journal Information:
Journal Volume: 5; Journal Issue: 10; Journal ID: ISSN 2375-2548
Publisher:
AAAS
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Garcia Quiroz, Felipe, Li, Nan K., Roberts, Stefan, Weber, Patrick, Dzuricky, Michael, Weitzhandler, Isaac, Yingling, Yaroslava G., and Chilkoti, Ashutosh. Intrinsically disordered proteins access a range of hysteretic phase separation behaviors. United States: N. p., 2019. Web. doi:10.1126/sciadv.aax5177.
Garcia Quiroz, Felipe, Li, Nan K., Roberts, Stefan, Weber, Patrick, Dzuricky, Michael, Weitzhandler, Isaac, Yingling, Yaroslava G., & Chilkoti, Ashutosh. Intrinsically disordered proteins access a range of hysteretic phase separation behaviors. United States. doi:https://doi.org/10.1126/sciadv.aax5177
Garcia Quiroz, Felipe, Li, Nan K., Roberts, Stefan, Weber, Patrick, Dzuricky, Michael, Weitzhandler, Isaac, Yingling, Yaroslava G., and Chilkoti, Ashutosh. Fri . "Intrinsically disordered proteins access a range of hysteretic phase separation behaviors". United States. doi:https://doi.org/10.1126/sciadv.aax5177. https://www.osti.gov/servlets/purl/1580951.
@article{osti_1580951,
title = {Intrinsically disordered proteins access a range of hysteretic phase separation behaviors},
author = {Garcia Quiroz, Felipe and Li, Nan K. and Roberts, Stefan and Weber, Patrick and Dzuricky, Michael and Weitzhandler, Isaac and Yingling, Yaroslava G. and Chilkoti, Ashutosh},
abstractNote = {The phase separation behavior of intrinsically disordered proteins (IDPs) is thought of as analogous to that of polymers that undergo equilibrium lower or upper critical solution temperature (LCST and UCST, respectively) phase transition. This view, however, ignores possible nonequilibrium properties of protein assemblies. Here, by studying IDP polymers (IDPPs) composed of repeat motifs that encode LCST or UCST phase behavior, we discovered that IDPs can access a wide spectrum of nonequilibrium, hysteretic phase behaviors. Experimentally and through simulations, we show that hysteresis in IDPPs is tunable and that it emerges through increasingly stable interchain interactions in the insoluble phase. To explore the utility of hysteretic IDPPs, we engineer self-assembling nanostructures with tunable stability. These findings shine light on the rich phase separation behavior of IDPs and illustrate hysteresis as a design parameter to program nonequilibrium phase behavior in self-assembling materials.},
doi = {10.1126/sciadv.aax5177},
journal = {Science Advances},
number = 10,
volume = 5,
place = {United States},
year = {2019},
month = {10}
}

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