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Title: Long-term nitrogen addition modifies microbial composition and functions for slow carbon cycling and increased sequestration in tropical forest soil

Abstract

Nitrogen (N) deposition is a component of global change that has considerable impact on belowground carbon (C) dynamics. Plant growth stimulation and alterations of fungal community composition and functions are the main mechanisms driving soil C gains following N deposition in N-limited temperate forests. In N-rich tropical forests, however, N deposition generally has minor effects on plant growth; consequently, C storage in soil may strongly depend on the microbial processes that drive litter and soil organic matter decomposition. Here, we investigated how microbial functions in old-growth tropical forest soil responded to 13 years of N addition at four rates: 0 (Control), 50 (Low-N), 100 (Medium-N), and 150 (High-N) kg N ha -1 year -1. Soil organic carbon (SOC) content increased under High-N, corresponding to a 33% decrease in CO 2 efflux, and reductions in relative abundances of bacteria as well as genes responsible for cellulose and chitin degradation. A 113% increase in N 2O emission was positively correlated with soil acidification and an increase in the relative abundances of denitrification genes (narG and norB). Soil acidification induced by N addition decreased available P concentrations, and was associated with reductions in the relative abundance of phytase. The decreased relative abundance of bacteria and keymore » functional gene groups for C degradation were related to slower SOC decomposition, indicating the key mechanisms driving SOC accumulation in the tropical forest soil subjected to High-N addition. However, changes in microbial functional groups associated with N and P cycling led to coincidentally large increases in N 2O emissions, and exacerbated soil P deficiency. These two factors partially offset the perceived beneficial effects of N addition on SOC storage in tropical forest soils. Finally, these findings suggest a potential to incorporate microbial community and functions into Earth system models considering their effects on greenhouse gas emission, biogeochemical processes, and biodiversity of tropical ecosystems.« less

Authors:
 [1];  [2];  [3]; ORCiD logo [4];  [2];  [3]; ORCiD logo [3]; ORCiD logo [5];  [6]; ORCiD logo [7]
  1. China Agricultural Univ., Beijing (China); Chinese Academy of Sciences (CAS), Beijing (China). Key Lab. of Ecosystem Network Observation and Modeling, Inst. of Geographic Sciences and Natural Resources Research
  2. Univ. of Exeter, Exeter (United Kingdom)
  3. Chinese Academy of Sciences (CAS), Guangzhou (China). Key Lab. of Vegetation Restoration and Management of Degraded Ecosystems and Guangdong Provincial Key Lab. of Applied Botany
  4. Tsinghua Univ., Beijing (China)
  5. Chinese Academy of Sciences (CAS), Beijing (China). Key Lab. of Ecosystem Network Observation and Modeling, Inst. of Geographic Sciences and Natural Resources Research
  6. Tsinghua Univ., Beijing (China). State Key Joint Lab. of Environment Simulation and Pollution Control; Univ. of Oklahoma, Norman, OK (United States). Inst. for Environmental Genomics; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  7. Univ. of Gottingen, Gottingen (Germany); Central South Univ. of Forestry and Technology, Changsha (China)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC); National Natural Science Foundation of China (NNSFC); National Key R&D Program of China
OSTI Identifier:
1580939
Grant/Contract Number:  
[AC02-05CH11231; 31770560; 41571130041; 41731176; 2017YFA0604803; LENOM2016Q0004]
Resource Type:
Accepted Manuscript
Journal Name:
Global Change Biology
Additional Journal Information:
[ Journal Volume: 25; Journal Issue: 10]; Journal ID: ISSN 1354-1013
Publisher:
Wiley
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; biogeochemical cycling; C and N turnover; global climate change; microbial functional community; N deposition; tropical forest

Citation Formats

Tian, Jing, Dungait, Jennifer A. J., Lu, Xiankai, Yang, Yunfeng, Hartley, Iain P., Zhang, Wei, Mo, Jiangming, Yu, Guirui, Zhou, Jizhong, and Kuzyakov, Yakov. Long-term nitrogen addition modifies microbial composition and functions for slow carbon cycling and increased sequestration in tropical forest soil. United States: N. p., 2019. Web. doi:10.1111/gcb.14750.
Tian, Jing, Dungait, Jennifer A. J., Lu, Xiankai, Yang, Yunfeng, Hartley, Iain P., Zhang, Wei, Mo, Jiangming, Yu, Guirui, Zhou, Jizhong, & Kuzyakov, Yakov. Long-term nitrogen addition modifies microbial composition and functions for slow carbon cycling and increased sequestration in tropical forest soil. United States. doi:10.1111/gcb.14750.
Tian, Jing, Dungait, Jennifer A. J., Lu, Xiankai, Yang, Yunfeng, Hartley, Iain P., Zhang, Wei, Mo, Jiangming, Yu, Guirui, Zhou, Jizhong, and Kuzyakov, Yakov. Fri . "Long-term nitrogen addition modifies microbial composition and functions for slow carbon cycling and increased sequestration in tropical forest soil". United States. doi:10.1111/gcb.14750.
@article{osti_1580939,
title = {Long-term nitrogen addition modifies microbial composition and functions for slow carbon cycling and increased sequestration in tropical forest soil},
author = {Tian, Jing and Dungait, Jennifer A. J. and Lu, Xiankai and Yang, Yunfeng and Hartley, Iain P. and Zhang, Wei and Mo, Jiangming and Yu, Guirui and Zhou, Jizhong and Kuzyakov, Yakov},
abstractNote = {Nitrogen (N) deposition is a component of global change that has considerable impact on belowground carbon (C) dynamics. Plant growth stimulation and alterations of fungal community composition and functions are the main mechanisms driving soil C gains following N deposition in N-limited temperate forests. In N-rich tropical forests, however, N deposition generally has minor effects on plant growth; consequently, C storage in soil may strongly depend on the microbial processes that drive litter and soil organic matter decomposition. Here, we investigated how microbial functions in old-growth tropical forest soil responded to 13 years of N addition at four rates: 0 (Control), 50 (Low-N), 100 (Medium-N), and 150 (High-N) kg N ha-1 year-1. Soil organic carbon (SOC) content increased under High-N, corresponding to a 33% decrease in CO2 efflux, and reductions in relative abundances of bacteria as well as genes responsible for cellulose and chitin degradation. A 113% increase in N2O emission was positively correlated with soil acidification and an increase in the relative abundances of denitrification genes (narG and norB). Soil acidification induced by N addition decreased available P concentrations, and was associated with reductions in the relative abundance of phytase. The decreased relative abundance of bacteria and key functional gene groups for C degradation were related to slower SOC decomposition, indicating the key mechanisms driving SOC accumulation in the tropical forest soil subjected to High-N addition. However, changes in microbial functional groups associated with N and P cycling led to coincidentally large increases in N2O emissions, and exacerbated soil P deficiency. These two factors partially offset the perceived beneficial effects of N addition on SOC storage in tropical forest soils. Finally, these findings suggest a potential to incorporate microbial community and functions into Earth system models considering their effects on greenhouse gas emission, biogeochemical processes, and biodiversity of tropical ecosystems.},
doi = {10.1111/gcb.14750},
journal = {Global Change Biology},
number = [10],
volume = [25],
place = {United States},
year = {2019},
month = {7}
}

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