Hexokinase is necessary for glucose-mediated photosynthesis repression and lipid accumulation in a green alga

Roth, Melissa S.; Westcott, Daniel J.; Iwai, Masakazu; Niyogi, Krishna K.

doi:10.1038/s42003-019-0577-1

Title: Hexokinase is necessary for glucose-mediated photosynthesis repression and lipid accumulation in a green alga

Journal Article · 19 September 2019 · Communications Biology

DOI: https://doi.org/10.1038/s42003-019-0577-1 · OSTI ID:1580873

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Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Global primary production is driven largely by oxygenic photosynthesis, with algae as major contributors. The green alga Chromochloris zofingiensis reversibly switches off photosynthesis in the presence of glucose in the light and augments production of biofuel precursors (triacylglycerols) and the high-value antioxidant astaxanthin. Here we used forward genetics to reveal that this photosynthetic and metabolic switch is mediated by the glycolytic enzyme hexokinase (CzHXK1). In contrast to wild-type, glucose-treated hxk1 mutants do not shut off photosynthesis or accumulate astaxanthin, triacylglycerols, or cytoplasmic lipid droplets. We show that CzHXK1 is critical for the regulation of genes related to photosynthesis, ketocarotenoid synthesis and fatty acid biosynthesis. Sugars play fundamental regulatory roles in gene expression, physiology, metabolism, and growth in plants and animals, and we introduce a relatively simple, emerging model system to investigate conserved eukaryotic sugar sensing and signaling at the base of the green lineage.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); University of California, Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC02-05CH11231; SC0018301

OSTI ID:: 1580873

Alternate ID(s):: OSTI ID: 1562686

Journal Information:: Communications Biology, Vol. 2, Issue 1; ISSN 2399-3642

Publisher:: Springer NatureCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 20 works

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