skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Hyporheic Zone Microbiome Assembly Is Linked to Dynamic Water Mixing Patterns in Snowmelt-Dominated Headwater Catchments

Abstract

Terrestrial and aquatic elemental cycles are tightly linked in upland fluvial networks. Mineral weathering, degradation of organic matter, and anthropogenic influences all result in the movement of solutes (e.g., carbon, metals, nutrients) through these catchments, with implications for downstream water quality. Within the river channel, the region of hyporheic mixing represents a hotspot of microbial activity, exerting significant control over solute cycling. To investigate how snowmelt-driven seasonal changes in river discharge affects the extent of hyporheic mixing, pore water biogeochemistry, and microbial community assembly, depth-resolved pore water samples were recovered from multiple locations around a representative meander on the East River near Crested Butte, CO, USA. Vertical temperature sensor arrays were also installed in the riverbed to enable seepage flux estimates. Snowmelt-driven high river discharge led to an expanding zone of vertical hyporheic mixing and introduced dissolved oxygen into the riverbed that stimulated microbial aerobic respiration. These physicochemical processes contributed to microbial communities undergoing homogenizing selection, in contrast to other ecosystems where lower permeability may limit the extent of mixing. Conversely, lower river discharge conditions led to a greater influence of upwelling groundwater within the riverbed and a decrease in microbial respiration rates. Associated with these processes, microbial communities throughoutmore » the riverbed exhibited increasing dissimilarity between each other, suggesting that the earlier onset of snowmelt and longer periods of baseflow may lead to changes in the composition (and associated function) of riverbed microbiomes, with subsequent implications for the processing and export of solutes from upland catchments.« less

Authors:
ORCiD logo [1];  [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1];  [2]; ORCiD logo [3]; ORCiD logo [4]; ORCiD logo [5]
  1. The Ohio State Univ., Columbus, OH (United States)
  2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  3. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL); Univ. of Arizona, Tucson, AZ (United States)
  4. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Rocky Mountain Biological Lab., Gothic, CO (United States)
  5. The Ohio State Univ., Columbus, OH (United States); Colorado State Univ., Fort Collins, CO (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1580868
Alternate Identifier(s):
OSTI ID: 1573270; OSTI ID: 1735737
Report Number(s):
PNNL-SA-152884
Journal ID: ISSN 2169-8953; ark:/13030/qt7hj7j6t4
Grant/Contract Number:  
AC02-05CH11231; SC0016488; AC05-76RL01830; AC02‐05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Geophysical Research. Biogeosciences
Additional Journal Information:
Journal Volume: 124; Journal Issue: 11; Journal ID: ISSN 2169-8953
Publisher:
American Geophysical Union
Country of Publication:
United States
Language:
English
Subject:
58 GEOSCIENCES; 59 BASIC BIOLOGICAL SCIENCES; seasonal hydrology; seasonal biogeochemistry; hyporheic biogeochemistry

Citation Formats

Saup, C. M., Bryant, S. R., Nelson, A. R., Harris, K. D., Sawyer, A. H., Christensen, J. N., Tfaily, M. M., Williams, K. H., and Wilkins, M. J. Hyporheic Zone Microbiome Assembly Is Linked to Dynamic Water Mixing Patterns in Snowmelt-Dominated Headwater Catchments. United States: N. p., 2019. Web. doi:10.1029/2019jg005189.
Saup, C. M., Bryant, S. R., Nelson, A. R., Harris, K. D., Sawyer, A. H., Christensen, J. N., Tfaily, M. M., Williams, K. H., & Wilkins, M. J. Hyporheic Zone Microbiome Assembly Is Linked to Dynamic Water Mixing Patterns in Snowmelt-Dominated Headwater Catchments. United States. doi:10.1029/2019jg005189.
Saup, C. M., Bryant, S. R., Nelson, A. R., Harris, K. D., Sawyer, A. H., Christensen, J. N., Tfaily, M. M., Williams, K. H., and Wilkins, M. J. Sat . "Hyporheic Zone Microbiome Assembly Is Linked to Dynamic Water Mixing Patterns in Snowmelt-Dominated Headwater Catchments". United States. doi:10.1029/2019jg005189. https://www.osti.gov/servlets/purl/1580868.
@article{osti_1580868,
title = {Hyporheic Zone Microbiome Assembly Is Linked to Dynamic Water Mixing Patterns in Snowmelt-Dominated Headwater Catchments},
author = {Saup, C. M. and Bryant, S. R. and Nelson, A. R. and Harris, K. D. and Sawyer, A. H. and Christensen, J. N. and Tfaily, M. M. and Williams, K. H. and Wilkins, M. J.},
abstractNote = {Terrestrial and aquatic elemental cycles are tightly linked in upland fluvial networks. Mineral weathering, degradation of organic matter, and anthropogenic influences all result in the movement of solutes (e.g., carbon, metals, nutrients) through these catchments, with implications for downstream water quality. Within the river channel, the region of hyporheic mixing represents a hotspot of microbial activity, exerting significant control over solute cycling. To investigate how snowmelt-driven seasonal changes in river discharge affects the extent of hyporheic mixing, pore water biogeochemistry, and microbial community assembly, depth-resolved pore water samples were recovered from multiple locations around a representative meander on the East River near Crested Butte, CO, USA. Vertical temperature sensor arrays were also installed in the riverbed to enable seepage flux estimates. Snowmelt-driven high river discharge led to an expanding zone of vertical hyporheic mixing and introduced dissolved oxygen into the riverbed that stimulated microbial aerobic respiration. These physicochemical processes contributed to microbial communities undergoing homogenizing selection, in contrast to other ecosystems where lower permeability may limit the extent of mixing. Conversely, lower river discharge conditions led to a greater influence of upwelling groundwater within the riverbed and a decrease in microbial respiration rates. Associated with these processes, microbial communities throughout the riverbed exhibited increasing dissimilarity between each other, suggesting that the earlier onset of snowmelt and longer periods of baseflow may lead to changes in the composition (and associated function) of riverbed microbiomes, with subsequent implications for the processing and export of solutes from upland catchments.},
doi = {10.1029/2019jg005189},
journal = {Journal of Geophysical Research. Biogeosciences},
number = 11,
volume = 124,
place = {United States},
year = {2019},
month = {10}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 2 works
Citation information provided by
Web of Science

Figures / Tables:

Figure 1 Figure 1: Location of Meander A and Sampling Locations A–C within the East River near Crested Butte, CO, part of the Upper Colorado River Basin.

Save / Share:

Works referenced in this record:

Geochemical Exports to River From the Intrameander Hyporheic Zone Under Transient Hydrologic Conditions: East River Mountainous Watershed, Colorado
journal, October 2018

  • Dwivedi, Dipankar; Steefel, Carl I.; Arora, Bhavna
  • Water Resources Research, Vol. 54, Issue 10
  • DOI: 10.1029/2018WR023377

Estimating and mapping ecological processes influencing microbial community assembly
journal, May 2015


Modeling Snowmelt Runoff Response to Climate Change in the Animas River Basin,Colorado
journal, January 2013


The use of strontium isotopes in determining groundwater mixing and brine fingering in a playa spring zone, Lake Tyrrell, Australia
journal, May 1995


Formularity: Software for Automated Formula Assignment of Natural and Other Organic Matter from Ultrahigh-Resolution Mass Spectra
journal, November 2017


A hydrogeomorphic river network model predicts where and why hyporheic exchange is important in large basins
journal, September 2014

  • Gomez-Velez, Jesus D.; Harvey, Judson W.
  • Geophysical Research Letters, Vol. 41, Issue 18
  • DOI: 10.1002/2014GL061099

An Ordination of the Upland Forest Communities of Southern Wisconsin
journal, February 1957

  • Bray, J. Roger; Curtis, J. T.
  • Ecological Monographs, Vol. 27, Issue 4
  • DOI: 10.2307/1942268

Quantitative strontium isotope models for weathering, pedogenesis and biogeochemical cycling
journal, February 1998


Microbial Community Cohesion Mediates Community Turnover in Unperturbed Aquifers
journal, July 2018


Deterministic influences exceed dispersal effects on hydrologically-connected microbiomes: Deterministic assembly of hyporheic microbiomes
journal, March 2017

  • Graham, Emily B.; Crump, Alex R.; Resch, Charles T.
  • Environmental Microbiology, Vol. 19, Issue 4
  • DOI: 10.1111/1462-2920.13720

Complete Genome of Ignavibacterium album, a Metabolically Versatile, Flagellated, Facultative Anaerobe from the Phylum Chlorobi
journal, January 2012


Characterization and Description of Anaeromyxobacter dehalogenans gen. nov., sp. nov., an Aryl-Halorespiring Facultative Anaerobic Myxobacterium
journal, February 2002


Thermodynamically controlled preservation of organic carbon in floodplains
journal, May 2017

  • Boye, Kristin; Noël, Vincent; Tfaily, Malak M.
  • Nature Geoscience, Vol. 10, Issue 6
  • DOI: 10.1038/ngeo2940

Biophysical controls on organic carbon fluxes in fluvial networks
journal, January 2008

  • Battin, Tom J.; Kaplan, Louis A.; Findlay, Stuart
  • Nature Geoscience, Vol. 1, Issue 2
  • DOI: 10.1038/ngeo101

Experimental warming causes large and rapid species loss, dampened by simulated grazing, on the Tibetan Plateau
journal, December 2004


Nutrient flux, uptake, and autotrophic limitation in streams and rivers
journal, March 2014

  • King, Sean A.; Heffernan, James B.; Cohen, Matthew J.
  • Freshwater Science, Vol. 33, Issue 1
  • DOI: 10.1086/674383

Stable Isotope Probing for Microbial Iron Reduction in Chocolate Pots Hot Spring, Yellowstone National Park
journal, March 2018

  • Fortney, Nathaniel W.; He, Shaomei; Kulkarni, Ajinkya
  • Applied and Environmental Microbiology, Vol. 84, Issue 11
  • DOI: 10.1128/AEM.02894-17

A multimodel ensemble approach to assessment of climate change impacts on the hydrology and water resources of the Colorado River basin
journal, January 2006

  • Christensen, N.; Lettenmaier, D. P.
  • Hydrology and Earth System Sciences Discussions, Vol. 3, Issue 6
  • DOI: 10.5194/hessd-3-3727-2006

Seasonal hyporheic dynamics control coupled microbiology and geochemistry in Colorado River sediments: COLORADO RIVER SEDIMENT BIOGEOCHEMISTRY
journal, December 2016

  • Danczak, Robert E.; Sawyer, Audrey H.; Williams, Kenneth H.
  • Journal of Geophysical Research: Biogeosciences, Vol. 121, Issue 12
  • DOI: 10.1002/2016JG003527

Hydrology controls dissolved organic matter export and composition in an Alpine stream and its hyporheic zone: Hydrology controls stream and hyporheic DOM
journal, December 2015

  • Fasching, Christina; Ulseth, Amber J.; Schelker, Jakob
  • Limnology and Oceanography, Vol. 61, Issue 2
  • DOI: 10.1002/lno.10232

How, When, and Where Relic DNA Affects Microbial Diversity
journal, June 2018


The East River, Colorado, Watershed: A Mountainous Community Testbed for Improving Predictive Understanding of Multiscale Hydrological–Biogeochemical Dynamics
journal, January 2018

  • Hubbard, Susan S.; Williams, Kenneth Hurst; Agarwal, Deb
  • Vadose Zone Journal, Vol. 17, Issue 1
  • DOI: 10.2136/vzj2018.03.0061

Arsenic geochemistry in a biostimulated aquifer: An aqueous speciation study: Arsenic geochemistry in a biostimulated aquifer
journal, April 2013

  • Stucker, Valerie K.; Williams, Kenneth H.; Robbins, Mark J.
  • Environmental Toxicology and Chemistry, Vol. 32, Issue 6
  • DOI: 10.1002/etc.2155

Impact of dust radiative forcing in snow on accuracy of operational runoff prediction in the Upper Colorado River Basin: DUST IN SNOW IMPACT ON STREAMFLOW
journal, August 2013

  • Bryant, Ann C.; Painter, Thomas H.; Deems, Jeffrey S.
  • Geophysical Research Letters, Vol. 40, Issue 15
  • DOI: 10.1002/grl.50773

Isotopic Studies of Contaminant Transport at the Hanford Site, Washington
journal, January 2007

  • Christensen, John N.; Conrad, Mark E.; DePaolo, Donald J.
  • Vadose Zone Journal, Vol. 6, Issue 4
  • DOI: 10.2136/vzj2006.0158

Hyporheic flow and transport processes: Mechanisms, models, and biogeochemical implications: HYPORHEIC FLOW AND TRANSPORT PROCESSES
journal, October 2014

  • Boano, F.; Harvey, J. W.; Marion, A.
  • Reviews of Geophysics, Vol. 52, Issue 4
  • DOI: 10.1002/2012RG000417

Graphical Method for Analysis of Ultrahigh-Resolution Broadband Mass Spectra of Natural Organic Matter, the Van Krevelen Diagram
journal, October 2003

  • Kim, Sunghwan; Kramer, Robert W.; Hatcher, Patrick G.
  • Analytical Chemistry, Vol. 75, Issue 20
  • DOI: 10.1021/ac034415p

Association of Arsenic and Phosphorus with Iron Nanoparticles between Streams and Aquifers: Implications for Arsenic Mobility
journal, November 2015

  • Hartland, Adam; Larsen, Joshua R.; Andersen, Martin S.
  • Environmental Science & Technology, Vol. 49, Issue 24
  • DOI: 10.1021/acs.est.5b03506

Global assessment of experimental climate warming on tundra vegetation: heterogeneity over space and time: Warming effects on tundra vegetation
journal, December 2011


Quantifying community assembly processes and identifying features that impose them
journal, June 2013

  • Stegen, James C.; Lin, Xueju; Fredrickson, Jim K.
  • The ISME Journal, Vol. 7, Issue 11
  • DOI: 10.1038/ismej.2013.93

Implications of global climate change for snowmelt hydrology in the twenty-first century
journal, March 2009

  • Adam, Jennifer C.; Hamlet, Alan F.; Lettenmaier, Dennis P.
  • Hydrological Processes, Vol. 23, Issue 7
  • DOI: 10.1002/hyp.7201

Denitrification in the Mississippi River network controlled by flow through river bedforms
journal, October 2015

  • Gomez-Velez, Jesus D.; Harvey, Judson W.; Cardenas, M. Bayani
  • Nature Geoscience, Vol. 8, Issue 12
  • DOI: 10.1038/ngeo2567

Colonization Habitat Controls Biomass, Composition, and Metabolic Activity of Attached Microbial Communities in the Columbia River Hyporheic Corridor
journal, June 2017

  • Stern, Noah; Ginder-Vogel, Matthew; Stegen, James C.
  • Applied and Environmental Microbiology, Vol. 83, Issue 16
  • DOI: 10.1128/AEM.00260-17

From mass to structure: an aromaticity index for high-resolution mass data of natural organic matter
journal, February 2006

  • Koch, B. P.; Dittmar, T.
  • Rapid Communications in Mass Spectrometry, Vol. 20, Issue 5
  • DOI: 10.1002/rcm.2386

Using strontium isotopes to evaluate the spatial variation of groundwater recharge
journal, October 2018


Variation in Rising Limb of Colorado River Snowmelt Runoff Hydrograph Controlled by Dust Radiative Forcing in Snow
journal, January 2018

  • Painter, Thomas H.; Skiles, S. McKenzie; Deems, Jeffrey S.
  • Geophysical Research Letters, Vol. 45, Issue 2
  • DOI: 10.1002/2017GL075826

From mass to structure: an aromaticity index for high-resolution mass data of natural organic matter
journal, December 2015

  • Koch, B. P.; Dittmar, T.
  • Rapid Communications in Mass Spectrometry, Vol. 30, Issue 1
  • DOI: 10.1002/rcm.7433

The ecology and biogeochemistry of stream biofilms
journal, March 2016

  • Battin, Tom J.; Besemer, Katharina; Bengtsson, Mia M.
  • Nature Reviews Microbiology, Vol. 14, Issue 4
  • DOI: 10.1038/nrmicro.2016.15

Erratum: Biophysical controls on organic carbon fluxes in fluvial networks
journal, July 2009

  • Battin, Tom J.; Kaplan, Louis A.; Findlay, Stuart
  • Nature Geoscience, Vol. 2, Issue 8
  • DOI: 10.1038/ngeo602

Spatial and Temporal Dynamics of Dissolved Oxygen Concentrations and Bioactivity in the Hyporheic Zone
journal, March 2018

  • Reeder, W. Jeffery; Quick, Annika M.; Farrell, Tiffany B.
  • Water Resources Research, Vol. 54, Issue 3
  • DOI: 10.1002/2017WR021388

Climate warming drives local extinction: Evidence from observation and experimentation
journal, February 2018

  • Panetta, Anne Marie; Stanton, Maureen L.; Harte, John
  • Science Advances, Vol. 4, Issue 2
  • DOI: 10.1126/sciadv.aaq1819

Pathways of arsenic from sediments to groundwater in the hyporheic zone: Evidence from an iron isotope study
journal, April 2014


The strontium isotope budget of the modern ocean
journal, February 1989


Estimating time-variable aerobic respiration in the streambed by combining electrical conductivity and dissolved oxygen time series: Variable Respiration in the Streambed
journal, August 2016

  • Vieweg, Michael; Kurz, Marie J.; Trauth, Nico
  • Journal of Geophysical Research: Biogeosciences, Vol. 121, Issue 8
  • DOI: 10.1002/2016JG003345

Plant Responses to Experimental Warming in a Montane Meadow
journal, March 2001


Response of Colorado River runoff to dust radiative forcing in snow
journal, September 2010

  • Painter, T. H.; Deems, J. S.; Belnap, J.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 40
  • DOI: 10.1073/pnas.0913139107

Degradation of natural organic matter: A thermodynamic analysis
journal, April 2011

  • LaRowe, Douglas E.; Van Cappellen, Philippe
  • Geochimica et Cosmochimica Acta, Vol. 75, Issue 8
  • DOI: 10.1016/j.gca.2011.01.020

    Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.