Conservation of Endophyte Bacterial Community Structure Across Two Panicum Grass Species
Abstract
Panicum represents a large genus of many North American prairie grass species. These include switchgrass (Panicum virgatum), a biofuel crop candidate with wide geographic range, as well as Panicum hallii, a close relative to switchgrass, which serves as a model system for the study of Panicum genetics due to its diploid genome and short growth cycles. For the advancement of switchgrass as a biofuel crop, it is essential to understand host microbiome interactions, which can be impacted by plant genetics and environmental factors inducing ecotype-specific phenotypic traits. We here compared rhizosphere and root endosphere bacterial communities of upland and lowland P. virgatum and P. hallii genotypes planted at two sites in Texas. Our analysis shows that sampling site predominantly contributed to bacterial community variance in the rhizosphere, however, impacted root endosphere bacterial communities much less. Instead we observed a relatively large core endophytic microbiome dominated by ubiquitously root-colonizing bacterial genera Streptomyces, Pseudomonas, and Bradyrhizobium. Endosphere communities displayed comparable diversity and conserved community structures across genotypes of both Panicum species. Functional insights into interactions between P. hallii and its root endophyte microbiome could hence inform testable hypotheses that are relevant for the improvement of switchgrass as a biofuel crop.
- Authors:
-
- USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
- Univ. of Texas, Austin, TX (United States)
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of Texas, Austin, TX (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
- OSTI Identifier:
- 1580863
- Alternate Identifier(s):
- OSTI ID: 1594755
- Grant/Contract Number:
- AC02-05CH11231; SC0014156
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Frontiers in Microbiology
- Additional Journal Information:
- Journal Volume: 10; Journal Issue: SEP; Journal ID: ISSN 1664-302X
- Publisher:
- Frontiers Research Foundation
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; plant microbiome; Panicum; rhizosphere; root endosphere; core microbiome; biofuel
Citation Formats
Singer, Esther, Bonnette, Jason, Woyke, Tanja, and Juenger, Thomas E. Conservation of Endophyte Bacterial Community Structure Across Two Panicum Grass Species. United States: N. p., 2019.
Web. doi:10.3389/fmicb.2019.02181.
Singer, Esther, Bonnette, Jason, Woyke, Tanja, & Juenger, Thomas E. Conservation of Endophyte Bacterial Community Structure Across Two Panicum Grass Species. United States. https://doi.org/10.3389/fmicb.2019.02181
Singer, Esther, Bonnette, Jason, Woyke, Tanja, and Juenger, Thomas E. Fri .
"Conservation of Endophyte Bacterial Community Structure Across Two Panicum Grass Species". United States. https://doi.org/10.3389/fmicb.2019.02181. https://www.osti.gov/servlets/purl/1580863.
@article{osti_1580863,
title = {Conservation of Endophyte Bacterial Community Structure Across Two Panicum Grass Species},
author = {Singer, Esther and Bonnette, Jason and Woyke, Tanja and Juenger, Thomas E.},
abstractNote = {Panicum represents a large genus of many North American prairie grass species. These include switchgrass (Panicum virgatum), a biofuel crop candidate with wide geographic range, as well as Panicum hallii, a close relative to switchgrass, which serves as a model system for the study of Panicum genetics due to its diploid genome and short growth cycles. For the advancement of switchgrass as a biofuel crop, it is essential to understand host microbiome interactions, which can be impacted by plant genetics and environmental factors inducing ecotype-specific phenotypic traits. We here compared rhizosphere and root endosphere bacterial communities of upland and lowland P. virgatum and P. hallii genotypes planted at two sites in Texas. Our analysis shows that sampling site predominantly contributed to bacterial community variance in the rhizosphere, however, impacted root endosphere bacterial communities much less. Instead we observed a relatively large core endophytic microbiome dominated by ubiquitously root-colonizing bacterial genera Streptomyces, Pseudomonas, and Bradyrhizobium. Endosphere communities displayed comparable diversity and conserved community structures across genotypes of both Panicum species. Functional insights into interactions between P. hallii and its root endophyte microbiome could hence inform testable hypotheses that are relevant for the improvement of switchgrass as a biofuel crop.},
doi = {10.3389/fmicb.2019.02181},
journal = {Frontiers in Microbiology},
number = SEP,
volume = 10,
place = {United States},
year = {2019},
month = {9}
}
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