Enhanced crude oil depletion by constructed bacterial consortium comprising bioemulsifier producer and petroleum hydrocarbon degraders

Xia, Mingqian; Fu, Dafang; Chakraborty, Romy; Singh, Rajendra Prasad; Terry, Norman

doi:10.1016/j.biortech.2019.01.131

Title: Enhanced crude oil depletion by constructed bacterial consortium comprising bioemulsifier producer and petroleum hydrocarbon degraders

Journal Article · 29 January 2019 · Bioresource Technology

DOI: https://doi.org/10.1016/j.biortech.2019.01.131 · OSTI ID:1580839

Xia, Mingqian ^[1]; Fu, Dafang ^[2]; Chakraborty, Romy ^[3]; Singh, Rajendra Prasad ^[2]; Terry, Norman ^[4]

Southeast Univ., Nanjing (China); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States)
Southeast Univ., Nanjing (China)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Univ. of California, Berkeley, CA (United States)

The aim of this work was to study the production of bioemulsifier by Rhodococcus erythropolis OSDS1, and the improvement of crude oil depletion efficiency using a consortium of petroleum hydrocarbon degraders and OSDS1. The results showed that R. erythropolis OSDS1 produced highly stable bioemulsifier under various salinity (0-35 g/L NaCl) and pH (5.0-9.0) conditions; more than 90% of the initial emulsification activity was retained after 168 h. Emulsification capacity of the bioemulsifier on different petroleum hydrocarbons was diesel > mineral oil/crude oil > gasoline. A mixed bacterial consortium combining OSDS1 and four other petroleum hydrocarbon degraders was constructed. GC-MS results revealed that the constructed consortium achieved 85.26% depletion efficiency of crude oil in 15 days, which was significantly higher than that of individual strains. During the process, we observed alkane hydroxylase gene (alkB) was successfully amplified from the consortium, confirming presence of crude oil degrading enzymes.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC); China Scholarship Council (CSC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1580839

Journal Information:: Bioresource Technology, Journal Name: Bioresource Technology Journal Issue: C Vol. 282; ISSN 0960-8524

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

References (43)

Emulsifier from a tropical marine yeast, Yarrowia lipolytica NCIM 3589 Zinjarde, Smita S.; Pant, Aditi Journal of Basic Microbiology, Vol. 42, Issue 1 https://doi.org/10.1002/1521-4028(200203)42:1<67::AID-JOBM67>3.0.CO;2-M	journal	March 2002
Biodegradation of Crude Oil by Individual Bacterial Strains and a Mixed Bacterial Consortium Isolated from Hydrocarbon Contaminated Areas Sathishkumar, Muthuswamy; Binupriya, Arthur Raj; Baik, Sang-Ho CLEAN – Soil, Air, Water, Vol. 36, Issue 1 https://doi.org/10.1002/clen.200700042	journal	January 2008
Production and applications of trehalose lipid biosurfactants Franzetti, Andrea; Gandolfi, Isabella; Bestetti, Giuseppina European Journal of Lipid Science and Technology, Vol. 112, Issue 6 https://doi.org/10.1002/ejlt.200900162	journal	April 2010
Production and Quantitative Analysis of Trehalose Lipid Biosurfactants Using High-Performance Liquid Chromatography Patil, Harshada I.; Pratap, Amit P. Journal of Surfactants and Detergents, Vol. 21, Issue 4 https://doi.org/10.1002/jsde.12158	journal	June 2018
Are alkane hydroxylase genes (alkB) relevant to assess petroleum bioremediation processes in chronically polluted coastal sediments? Paisse, Sandrine; Duran, Robert; Coulon, Frédéric Applied Microbiology and Biotechnology, Vol. 92, Issue 4 https://doi.org/10.1007/s00253-011-3381-5	journal	June 2011
Interspecific interactions in mixed microbial cultures in a biodegradation perspective Mikesková, H.; Novotný, Č.; Svobodová, K. Applied Microbiology and Biotechnology, Vol. 95, Issue 4 https://doi.org/10.1007/s00253-012-4234-6	journal	June 2012
Isolation and Characterization of Novel Strains of Pseudomonas aeruginosa and Serratia marcescens Possessing High Efficiency to Degrade Gasoline, Kerosene, Diesel Oil, and Lubricating Oil Wongsa, Patcharaporn; Tanaka, Makiko; Ueno, Akio Current Microbiology, Vol. 49, Issue 6 https://doi.org/10.1007/s00284-004-4347-y	journal	December 2004
Microaerophilic alkane degradation in Pseudomonas extremaustralis : a transcriptomic and physiological approach Tribelli, Paula M.; Rossi, Leticia; Ricardi, Martiniano M. Journal of Industrial Microbiology and Biotechnology, Vol. 45, Issue 1 https://doi.org/10.1007/s10295-017-1987-z	journal	January 2018
Study Potential of Indigenous Pseudomonas aeruginosa and Bacillus subtilis in Bioremediation of Diesel-Contaminated Water Safdari, Mohammad-Saeed; Kariminia, Hamid-Reza; Ghobadi Nejad, Zahra Water, Air, & Soil Pollution, Vol. 228, Issue 1 https://doi.org/10.1007/s11270-016-3220-5	journal	December 2016
High molecular weight bioemulsifiers, main properties and potential environmental and biomedical applications Mnif, Inès; Ghribi, Dhouha World Journal of Microbiology and Biotechnology, Vol. 31, Issue 5 https://doi.org/10.1007/s11274-015-1830-5	journal	March 2015
Characterization of biosurfactants produced by the oil-degrading bacterium Rhodococcus erythropolis S67 at low temperature Luong, T. M.; Ponamoreva, O. N.; Nechaeva, I. A. World Journal of Microbiology and Biotechnology, Vol. 34, Issue 2 https://doi.org/10.1007/s11274-017-2401-8	journal	January 2018
Biosurfactants of Rhodococcus erythropolis IMV Ас-5017: Synthesis Intensification and Practical Application Pirog, Tetyana; Sofilkanych, Anna; Shevchuk, Tetyana Applied Biochemistry and Biotechnology, Vol. 170, Issue 4 https://doi.org/10.1007/s12010-013-0246-7	journal	April 2013
A Comprehensive Review of Aliphatic Hydrocarbon Biodegradation by Bacteria Abbasian, Firouz; Lockington, Robin; Mallavarapu, Megharaj Applied Biochemistry and Biotechnology, Vol. 176, Issue 3 https://doi.org/10.1007/s12010-015-1603-5	journal	May 2015
The Pseudomonas oleovorans alkane hydroxylase gene Kok, M.; Oldenhuis, R.; van der Linden, M. P. G. Journal of Biological Chemistry, Vol. 264, Issue 10 https://doi.org/10.1016/S0021-9258(18)83564-5	journal	April 1989
Biosurfactants and oil bioremediation Ron, Eliora Z.; Rosenberg, Eugene Current Opinion in Biotechnology, Vol. 13, Issue 3 https://doi.org/10.1016/S0958-1669(02)00316-6	journal	June 2002
Towards efficient crude oil degradation by a mixed bacterial consortium Rahman, K. S. M.; Thahira-Rahman, J.; Lakshmanaperumalsamy, P. Bioresource Technology, Vol. 85, Issue 3 https://doi.org/10.1016/S0960-8524(02)00119-0	journal	December 2002
A study on the structure–function relationship of lipopeptide biosurfactants Morikawa, Masaaki; Hirata, Yoshihiko; Imanaka, Tadayuki Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol. 1488, Issue 3 https://doi.org/10.1016/S1388-1981(00)00124-4	journal	November 2000
Production and properties of a surfactant obtained from Bacillus subtilis grown on cassava wastewater Nitschke, Marcia; Pastore, Glaucia Maria Bioresource Technology, Vol. 97, Issue 2 https://doi.org/10.1016/j.biortech.2005.02.044	journal	January 2006
Isolation of an alkane-degrading Alcanivorax sp. strain 2B5 and cloning of the alkB gene Liu, Yi-Chen; Li, Ling-Zhi; Wu, Ying Bioresource Technology, Vol. 101, Issue 1 https://doi.org/10.1016/j.biortech.2009.08.028	journal	January 2010
Microbial degradation of petroleum hydrocarbons Varjani, Sunita J. Bioresource Technology, Vol. 223 https://doi.org/10.1016/j.biortech.2016.10.037	journal	January 2017
Biodegradation of crude oil by a defined co-culture of indigenous bacterial consortium and exogenous Bacillus subtilis Tao, Kaiyun; Liu, Xiaoyan; Chen, Xueping Bioresource Technology, Vol. 224 https://doi.org/10.1016/j.biortech.2016.10.073	journal	January 2017
Improved polycyclic aromatic hydrocarbon degradation in a crude oil by individual and a consortium of bacteria Kumari, Smita; Regar, Raj Kumar; Manickam, Natesan Bioresource Technology, Vol. 254 https://doi.org/10.1016/j.biortech.2018.01.075	journal	April 2018
Characterization of biosurfactants produced by novel strains of Ochrobactrum anthropi HM-1 and Citrobacter freundii HM-2 from used engine oil-contaminated soil Ibrahim, Haytham M. M. Egyptian Journal of Petroleum, Vol. 27, Issue 1 https://doi.org/10.1016/j.ejpe.2016.12.005	journal	March 2018
Simultaneous production of biosurfactant and ULSD (ultra low sulfur diesel) using Rhodococcus sp. in a chemostat Bandyopadhyay, Sujaya; Chowdhury, Ranjana; Bhattacharjee, Chiranjib Fuel, Vol. 113 https://doi.org/10.1016/j.fuel.2013.05.036	journal	November 2013
Biodegradation ability and dioxgenase genes of PAH-degrading Sphingomonas and Mycobacterium strains isolated from mangrove sediments Guo, Chuling; Dang, Zhi; Wong, Yukshan International Biodeterioration & Biodegradation, Vol. 64, Issue 6 https://doi.org/10.1016/j.ibiod.2010.04.008	journal	September 2010
High efficiency degradation of alkanes and crude oil by a salt-tolerant bacterium Dietzia species CN-3 Chen, Weiwei; Li, Junde; Sun, Xiangnan International Biodeterioration & Biodegradation, Vol. 118 https://doi.org/10.1016/j.ibiod.2017.01.029	journal	March 2017
Crude oil depletion by bacterial strains isolated from a petroleum hydrocarbon impacted solid waste management site in California Xia, Mingqian; Liu, Yi; Taylor, Alicia A. International Biodeterioration & Biodegradation, Vol. 123 https://doi.org/10.1016/j.ibiod.2017.06.003	journal	September 2017
A new method for the detection of alkane-monooxygenase homologous genes (alkB) in soils based on PCR-hybridization Kloos, Karin; Munch, Jean Charles; Schloter, Michael Journal of Microbiological Methods, Vol. 66, Issue 3 https://doi.org/10.1016/j.mimet.2006.01.014	journal	September 2006
Production and properties of a bioemulsifier synthesized by phenanthrene-degrading Penicillium sp. Luna-Velasco, M. Antonia; Esparza-García, Fernando; Cañízares-Villanueva, R. Olivia Process Biochemistry, Vol. 42, Issue 3 https://doi.org/10.1016/j.procbio.2006.08.015	journal	March 2007
Effect of micellar solubilization on biodegradation rates of hydrocarbons Bury, Scott J.; Miller, Clarence A. Environmental Science & Technology, Vol. 27, Issue 1 https://doi.org/10.1021/es00038a010	journal	January 1993
Enhanced Biodegradation of Casablanca Crude Oil by A Microbial Consortium in Presence of a Rhamnolipid Produced by Pseudomonas Aeruginosa AT10 Abalos, A.; Viñas, M.; Sabaté, J. Biodegradation, Vol. 15, Issue 4 https://doi.org/10.1023/B:BIOD.0000042915.28757.fb	journal	August 2004
Functional gene diversity of soil microbial communities from five oil-contaminated fields in China Liang, Yuting; Van Nostrand, Joy D.; Deng, Ye The ISME Journal, Vol. 5, Issue 3 https://doi.org/10.1038/ismej.2010.142	journal	September 2010
Bacterial responses to osmotic challenges Wood, Janet M. Journal of General Physiology, Vol. 145, Issue 5 https://doi.org/10.1085/jgp.201411296	journal	April 2015
Rhodococcus sovatensis sp. nov., an actinomycete isolated from the hypersaline and heliothermal Lake Ursu Táncsics, András; Máthé, István; Benedek, Tibor International Journal of Systematic and Evolutionary Microbiology, Vol. 67, Issue 2 https://doi.org/10.1099/ijsem.0.001514	journal	February 2017
Simultaneous hydrocarbon biodegradation and biosurfactant production by oilfield-selected bacteria: Hydrocarbon biodegradation and biosurfactant production Mnif, S.; Chamkha, M.; Labat, M. Journal of Applied Microbiology, Vol. 111, Issue 3 https://doi.org/10.1111/j.1365-2672.2011.05071.x	journal	July 2011
Degradation of alkanes by bacteria Rojo, Fernando Environmental Microbiology, Vol. 11, Issue 10 https://doi.org/10.1111/j.1462-2920.2009.01948.x	journal	October 2009
Anaerobic Degradation of Benzene, Toluene, Ethylbenzene, and Xylene Compounds by Dechloromonas Strain RCB Chakraborty, Romy; O'Connor, Susan M.; Chan, Emily Applied and Environmental Microbiology, Vol. 71, Issue 12 https://doi.org/10.1128/AEM.71.12.8649-8655.2005	journal	December 2005
Bioemulsifier Production in Batch Culture Using Glucose as Carbon Source by Candida lipolytica Sarubbo, Leonie A.; Marçal, Maria Do Carmo; Neves, Maria Luisa C. Applied Biochemistry and Biotechnology, Vol. 95, Issue 1 https://doi.org/10.1385/ABAB:95:1:59	journal	January 2001
Biosurfactant production by Rhodococcus erythropolis and its application to oil removal Pacheco, Graziela Jardim; Ciapina, Elisa Mara Prioli; Gomes, Edelvio de Barros Brazilian Journal of Microbiology, Vol. 41, Issue 3 https://doi.org/10.1590/S1517-83822010000300019	journal	January 2010
Biosurfactant produced by a Rhodococcus erythropolis mutant as an oil spill response agent Cai, Qinhong; Zhang, Baiyu; Chen, Bing Water Quality Research Journal, Vol. 51, Issue 2 https://doi.org/10.2166/wqrjc.2016.025	journal	January 2016
Production and stability studies of the bioemulsifier obtained from a new strain of Candida glabrata UCP 1002 Asfora Sarubbo, Leonie; Moura De Luna, Juliana; De Campos-Takaki, Galba Maria Electronic Journal of Biotechnology, Vol. 9, Issue 4 https://doi.org/10.2225/vol9-issue4-fulltext-6	journal	July 2006
Bioemulsifiers are not biosurfactants and require different screening approaches Uzoigwe, Chibuzo; Burgess, J. Grant; Ennis, Christopher J. Frontiers in Microbiology, Vol. 6 https://doi.org/10.3389/fmicb.2015.00245	journal	April 2015
Construction and Evaluation of a Korean Native Microbial Consortium for the Bioremediation of Diesel Fuel-Contaminated Soil in Korea Lee, Yunho; Jeong, Sang Eun; Hur, Moonsuk Frontiers in Microbiology, Vol. 9 https://doi.org/10.3389/fmicb.2018.02594	journal	October 2018

Cited By (6)

Designer bioemulsifiers based on combinations of different polysaccharides with the novel emulsifying esterase AXE from Bacillus subtilis CICC 20034 Tao, Weiyi; Lin, Junzhang; Wang, Weidong Microbial Cell Factories, Vol. 18, Issue 1 https://doi.org/10.1186/s12934-019-1221-y	journal	October 2019
One Biosurfactant-Producing Bacteria Achromobacter sp. A-8 and Its Potential Use in Microbial Enhanced Oil Recovery and Bioremediation Deng, Zhenshan; Jiang, Yingying; Chen, Kaikai Frontiers in Microbiology, Vol. 11 https://doi.org/10.3389/fmicb.2020.00247	journal	February 2020
Hydrocarbon Removal by Two Differently Developed Microbial Inoculants and Comparing Their Actions with Biostimulation Treatment Brzeszcz, Joanna; Kapusta, Piotr; Steliga, Teresa Molecules, Vol. 25, Issue 3 https://doi.org/10.3390/molecules25030661	journal	February 2020
Crude Oil Biodegradation by Newly Isolated Bacterial Strains and Their Consortium Under Soil Microcosm Experiment Bidja Abena, Marie Thérèse; Sodbaatar, Naranjargal; Li, Tongtong Applied Biochemistry and Biotechnology, Vol. 189, Issue 4 https://doi.org/10.1007/s12010-019-03058-2	journal	June 2019
Assessment of biosurfactant-producing bacteria from oil contaminated soils and their hydrocarbon degradation potential Soltanighias, Tayebeh; Singh, Athoiba E. A.; Satpute, Surekha K. Environmental Sustainability, Vol. 2, Issue 3 https://doi.org/10.1007/s42398-019-00074-0	journal	June 2019
Hydrocarbon Removal by Two Differently Developed Microbial Inoculants and Comparing Their Actions with Biostimulation Treatment Brzeszcz, Joanna; Kapusta, Piotr; Steliga, Teresa Molecules, Vol. 25, Issue 3 https://doi.org/10.3390/molecules25030661	journal	February 2020

Similar Records

Effect of emulsan on biodegradation of crude oil by pure and mixed bacterial cultures

Journal Article · 1989 · Applied and Environmental Microbiology; (USA) · OSTI ID:5028107

Foght, J M; Westlake, D W.S.; Gutnick, D L

Production, Characterization and Bioemulsifying Activity of an Exopolysaccharide Produced by Sphingomonas sp. Isolated from Freshwater

Journal Article · 2017 · Journal of Polymers and the Environment · OSTI ID:22788378

Meneghine, Aylan Kener; Moretto, Cristiane; Castellane, Tereza Cristina Luque

Morphological and biochemical characteristics of bacterial isolates degrading crude oil

Journal Article · 1993 · Journal of Environmental Science and Health, Part A: Environmental Science and Engineering; (United States) · OSTI ID:5752901

Janiyani, K L; Wate, S R; Joshi, S R

Related Subjects

02 PETROLEUM
54 ENVIRONMENTAL SCIENCES
59 BASIC BIOLOGICAL SCIENCES
Rhodococcus erythropolis
alkB gene
bioemulsifier
constructed bacterial consortium
crude oil depletion

Title: Enhanced crude oil depletion by constructed bacterial consortium comprising bioemulsifier producer and petroleum hydrocarbon degraders

Citation Formats

References (43)

Cited By (6)

Similar Records

Related Subjects